Name: Mycobacterium moriokaense DSM 44221
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 8467

Type strain:

Species: Mycobacterium moriokaense

Culture col. no.: DSM 44221, ATCC 43059, JCM 6375, CCUG 37671, CIP 105393, KCTC 9516, VKM Ac-1183

Strain history: M. Tsukamura E11715 (=48504).

NCBI tax ID(s): 39691 (species)

Section

Mycobacterium moriokaense DSM 44221 is an aerobe, mesophilic human pathogen that was isolated from soil.

aerobe
human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Mycobacteriaceae*
	Genus	*Mycobacterium*
	Species	**Mycobacterium moriokaense**
	Full Scientific Name (PNU)	Mycobacterium moriokaense Tsukamura et al. 1986

Synonym

Mycolicibacterium moriokaense

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69480]	Motility	no Confidence in %91.04

[Ref.: #20020]	Colony color	Oyster white (1013)
[Ref.: #20020]	Incubation period	10-14 days
[Ref.: #20020]	Cultivation medium used	5006

[Ref.: #20020]	Colony color	Oyster white (1013)
[Ref.: #20020]	Cultivation medium used	MB7H10

[Ref.: #20020]	Colony color	Oyster white (1013)
[Ref.: #20020]	Incubation period	10-14 days
[Ref.: #20020]	Cultivation medium used	MB7H11

[Ref.: #53798]	Incubation period	2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #11605]

Culture medium

LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354)

[Ref.: #11605]

Culture medium growth

[Ref.: #11605]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #11605]

Culture medium composition

expand / minimize

[Ref.: #11605]

Culture medium

MIDDLEBROOK MEDIUM (DSMZ Medium 645)

[Ref.: #11605]

Culture medium growth

[Ref.: #11605]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #11605]

Culture medium composition

expand / minimize

[Ref.: #20020]

Culture medium

MB7H10

[Ref.: #20020]

Culture medium growth

[Ref.: #20020]

Culture medium composition

expand / minimize

[Ref.: #20020]

Culture medium

MB7H11

[Ref.: #20020]

Culture medium growth

[Ref.: #20020]

Culture medium composition

expand / minimize

[Ref.: #20020]

Culture medium

5006

[Ref.: #20020]

Culture medium growth

[Ref.: #20020]

Culture medium composition

expand / minimize

[Ref.: #36368]

Culture medium

MEDIUM 55 - for Mycobacterium

[Ref.: #36368]

Culture medium growth

		Temperature
[Ref.: #11605]	growth	37 °C
[Ref.: #20020]	optimum	37 °C
[Ref.: #36368]	growth	37 °C
[Ref.: #53798]	growth	30-37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #11605]	Temperature range	mesophilic
[Ref.: #20020]	Temperature range	mesophilic
[Ref.: #36368]	Temperature range	mesophilic
[Ref.: #53798]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #53798]

Oxygen tolerance

aerobe

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.901

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym, API Coryne
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym, API Coryne
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API Coryne
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym, API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #20020]	390	-	+	+	-	-	-	+	-	-	+	-	not determinedn.d.	-	-	-	-	-	-	+	-	not determinedn.d.
[Ref.: #11605]	3986	+	+/-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #20020]	809	not determinedn.d.	-	+	+	-	+	+	+	+	+	+	-	-	-	-	+	+	-	+	-
[Ref.: #11605]	6195	-	-	+/-	+	-	+	-	+/-	+/-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11605]	Sample type/isolated from	soil
[Ref.: #11605]	Country	Japan
[Ref.: #11605]	Country ISO 3 Code	JPN
[Ref.: #11605]	Continent	Asia

[Ref.: #53798]	Sample type/isolated from	Soil

[Ref.: #67770]	Sample type/isolated from	Soil
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11605]	Pathogenicity (human)	yes, in single cases
[Ref.: #11605]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #20020]	Biosafety level	1	German classification

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #11605]	Mycobacterium moriokaense 16S rRNA gene, strain DSM 44221T	AJ429044	1493	ENA	39691 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Mycolicibacterium moriokaense CIP105393	GCA_002086395	contig	GenBank	39691 tax ID
[Ref.: #66792]	Mycolicibacterium moriokaense DSM 44221	GCA_025822185	contig	GenBank	39691 tax ID	*
[Ref.: #66792]	Mycobacterium moriokaense strain CIP105393	39691.4	wgs	PATRIC	39691 tax ID	*
[Ref.: #66792]	Mycobacterium moriokaense strain CIP105393	39691.3	wgs	PATRIC	39691 tax ID	*
[Ref.: #66792]	Mycolicibacterium moriokaense strain DSM 44221	39691.9	wgs	PATRIC	39691 tax ID	*
[Ref.: #66792]	Mycolicibacterium moriokaense strain JCM 6375	39691.13	complete	PATRIC	39691 tax ID	*

[Ref.: #67770]	GC-content	64 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	66 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Mycolicibacterium moriokaense JCM 6375 NCBI: GCA_010726085.1
[Ref.: #69480]	gram-positive	yes	100	no
[Ref.: #69480]	spore-forming	no	99.901	yes
[Ref.: #69480]	anaerobic	no	99.285	no
[Ref.: #69480]	thermophile	no	99.333	no
[Ref.: #69480]	motile	no	91.04	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Mycolicibacterium moriokaense JCM 6375 NCBI: GCA_010726085.1
[Ref.: #69481]	spore-forming	no	86	no

Availability in culture collections External links

[Ref.: #11605]

Culture collection no.

DSM 44221, ATCC 43059, JCM 6375, CCUG 37671, CIP 105393, KCTC 9516, VKM Ac-1183

[Ref.: #20218]

Associated Passport(s) in StrainInfo

153034, 112070, 139549

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Phenotypic and molecular characterisation of a novel species, Mycobacterium hubeiense sp., isolated from the sputum of a patient with secondary tuberculosis in Hubei of China.	Yu X, Zheng H, Zhou F, Hu P, Wang H, Li N, He J, Wang P, Zhang L, Men H, Xiang J, Zhang S	Epidemiol Infect	10.1017/S0950268820000436	2020	*
Phylogeny	Mycolicibacterium stellerae sp. nov., a rapidly growing scotochromogenic strain isolated from Stellera chamaejasme.	Nouioui I, Sangal V, Cortes-Albayay C, Jando M, Igual JM, Klenk HP, Zhang YQ, Goodfellow M	Int J Syst Evol Microbiol	10.1099/ijsem.0.003644	2019	*
Phylogeny	Genome-guided and mass spectrometry investigation of natural products produced by a potential new actinobacterial strain isolated from a mangrove ecosystem in Futian, Shenzhen, China.	Hu D, Gao C, Sun C, Jin T, Fan G, Mok KM, Lee SM	Sci Rep	10.1038/s41598-018-37475-w	2019	*

References

#11605

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44221

#20020

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#36368 ; Curators of the CIP;
#53798 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 37671

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Mycolicibacterium moriokaense JCM 6375

Mycolicibacterium moriokaense JCM 6375

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