Name: Middlebrook 7H9 (MB7H9) Broth Base
Middelbrook 7H9 Broth Base 4.7 g/l
Glycerol 2.0 g/l
Casaminoacids 11.0 g/l
Yeast extract 5.5 g/l
Tween80 (20%) 2.5 g/l
OADC-Enrichment 100 ml
Agar 15.0 g/l
Preparation: Dissolve in Milli-Q-H2O. Before Use add 10% OADC stock solution
Sterilisation: 20 minutes at 121°C
pH before sterilisation:
Maintenance and revitalization
OADC stock solution
old broth-name: OADC-Stock
Bovine Serum Albumin, fraction V 5 %
1% Oleic Acid in 0.2N NaOH 5ml
Glucose 2 %
NaCl 0.85 %
Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this.
Sterilisation: 20 minutes Temperature:121°C
Biological details Culture type:
suitable for: Mycobacteria
Japan Collection of Microorganism (JCM) ; Curators of the JCM;
Automatically annotated from API Coryne .
Automatically annotated from API zym .
* These data were automatically processed and therefore are not curated
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