Mycobacterium diernhoferi (DSM 43524, ATCC 19340, JCM 6371)

Name: Mycobacterium diernhoferi (DSM 43524, ATCC 19340, JCM 6371)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 8226

Type strain

Strain Designation 14 117 0001

Culture col. no. DSM 43524 ATCC 19340 JCM 6371 SN 1418 BCRC 16395 8 more

NCBI tax ID(s) 1801

Special Collections DSMZ JCM KCTC CIP

History DSMZ <- I. Tarnok, SN 1418 <- K. Diernhofer <- M. Tsukamura, 41001 JCM <-- M. Tsukamura 41001 <-- ATCC 19340 <-- R. Bonicke SN 1418 <-- K. Diernhofer. CIP <- 1998, V. Vincent, Inst. Pasteur, Paris, France: strain 14 117 0001 <- ATCC <- R. Bonicke: strain SN 1418 <- K. Diernhofer

Links

Mycobacterium diernhoferi 14 117 0001 is an obligate aerobe, rod-shaped bacterium that was isolated from drinking trough for cows.

rod-shaped obligate aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 92857

CT1967-00993,ATCC 19340,CIP 105384,ATCC 41001,DSM 43524,JCM 6371,IFO 14756,KCTC 9506,NBRC 14756,HAMBI 2269,BCRC 16395,CCRC 16395,CGMCC 4.1179,CNCTC 7265

@ref 20215

Last LPSN update 2026-05-29 08:26:49

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Mycobacteriales

Family Mycobacteriaceae

Genus Mycobacterium

Species Mycobacterium diernhoferi

Full scientific name Mycobacterium diernhoferi (ex Bönicke and Juhasz 1965) Tsukamura et al. 1983

Synonyms (1)

Mycolicibacterium diernhoferi

BacDive ID	Other strains from **Mycobacterium diernhoferi** (8)	Type strain
8220	M. diernhoferi Nr. 61028/I, DSM 43218, SN 1403
8221	M. diernhoferi DSM 43492, SN 291
8222	M. diernhoferi DSM 43520, IMET 10674, SN 1401
8223	M. diernhoferi 61214, DSM 43521, IMET 10675, SN 1406
8224	M. diernhoferi 61028/I, 62003/II Ba, DSM 43522, IMET 10676, ...
8225	M. diernhoferi DSM 43523, SN 1413
8227	M. diernhoferi DSM 43525, ATCC 19342, SN 1421
146813	M. diernhoferi CCUG 32168, TMC 1540

Morphology

Cell morphology

@ref	Cell shape	Gram stain	Confidence
118931	rod-shaped
125439		positive	93.088
125438			90

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11112	LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354)	Medium recipe at MediaDive	Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water
36369	MEDIUM 55 - for Mycobacterium
118931	CIP Medium 55	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
11112	positive	growth	37
36369	positive	growth	37
67770	positive	growth	37
118931	positive	growth	22-37
118931	negative	growth	10
118931	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
118931	obligate aerobe
125439	obligate aerobe	98.628

Observation

67770

Observationquinones: MK-9(H₂)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
118931	606565 ChEBI	hippurate	-	hydrolysis
68369	25115 ChEBI	malate	-	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
118931	17632 ChEBI	nitrate	+	reduction
118931	17632 ChEBI	nitrate	-	respiration
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
118931	16301 ChEBI	nitrite	-	reduction
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	+	hydrolysis	from API 20NE

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE
118931	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
118931	amylase	-
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
118931	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
118931	caseinase	-	3.4.21.50
118931	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
118931	DNase	-
68382	esterase lipase (C 8)	+		from API zym
118931	gamma-glutamyltransferase	-	2.3.2.2
118931	gelatinase	+/-
68369	gelatinase	-		from API 20NE
118931	lecithinase	+
68382	leucine arylamidase	+	3.4.11.1	from API zym
118931	lipase	-
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
118931	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
118931	tween esterase	-
118931	urease	+	3.5.1.5
68369	urease	+	3.5.1.5	from API 20NE
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
11112	-	-	+/-	+	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-
11112	-	-	+/-	+	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-
118931	-	-	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
11112	+	-	-	-	+	+/-	-	-	+/-	+/-	-	+/-	-	-	+/-	-	+/-	-	+/-	-	not determinedn.d.
11112	+	-	-	-	+	+	-	-	+/-	+/-	-	+	-	-	+	-	-	-	+/-	-	not determinedn.d.

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
118931	not determinedn.d.	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	-	-	+	-	-	-	-	-	+	-	-	-	+	-	-	+	-	-	-	-	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Mammals	#Bovinae (Cow, Cattle)

Isolation

@ref	Sample type
11112	drinking trough for cows
67770	Drinking trough for cows
118931	Drinking trough for cows

Taxonmaps

69479

Global distribution of 16S sequence AF480599 (>99% sequence identity) for Mycolicibacterium from Microbeatlas

Aquatic Samples	26
Soil Samples	22
Animal Samples	16
Plant Samples	6
Total Samples	70

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11112	1	Risk group (German classification) According to TRBA 466
118931	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1945665v1 assembly for Mycolicibacterium diernhoferi ATCC 19340	complete	GCA_019456655	1801.9	8111848642	1801	98.53
66792	ASM2582238v1 assembly for Mycolicibacterium diernhoferi DSM 43524	contig	GCA_025822385	1801.12	8115292048	1801	66.86
124043	ASM3953320v1 assembly for Mycolicibacterium diernhoferi JCM 6371	scaffold	GCA_039533205			1801	58.64

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Mycobacterium diernhoferi 16S ribosomal RNA gene, partial sequence	AF480599	1458	1801
20218	M.diernhoferi 16S ribosomal RNA, part	X55593	1458	1801
20218	Mycobacterium diernhoferi strain CIP 105384 16S ribosomal RNA gene, partial sequence	AF547916	540	1801

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM1945665v1 assembly for Mycolicibacterium diernhoferi ATCC 19340
BV-BRC: 1801.9

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.63	no
125439	gram_stain	BacteriaNetⓘ	positive	93.09	no
125439	motility	BacteriaNetⓘ	no	86.42	no
125439	spore_formation	BacteriaNetⓘ	no	84.58	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Mycobacterium diernhoferi ATCC 19340
NCBI: GCA_019456655.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	89.09	no
125438	anaerobic	anaerobicⓘ	no	97.76	yes
125438	aerobic	aerobicⓘ	yes	89.31	yes
125438	spore-forming	spore-formingⓘ	no	59.77	no
125438	thermophilic	thermophileⓘ	no	96.00	yes
125438	flagellated	motile2+ⓘ	no	90.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Peptide aMptD-mediated capture PCR for detection of Mycobacterium avium subsp. paratuberculosis in bulk milk samples.	Stratmann J, Dohmann K, Heinzmann J, Gerlach GF.	Appl Environ Microbiol	10.1128/aem.00590-06	2006
Phylogeny	Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera.	Gupta RS, Lo B, Son J.	Front Microbiol	10.3389/fmicb.2018.00067	2018
	Complete Genome Sequences of 14 Nontuberculous Mycobacteria Type Strains.	Igarashi Y, Osugi A, Murase Y, Chikamatsu K, Shimomura Y, Hosoya M, Aono A, Morishige Y, Yamada H, Takaki A, Mitarai S.	Microbiol Resour Announc	10.1128/mra.01214-22	2023
	In Vitro Antimicrobial Activities of Tigecycline, Eravacycline, Omadacycline, and Sarecycline against Rapidly Growing Mycobacteria.	Zhang T, Du J, Dong L, Wang F, Zhao L, Jia J, Wang C, Cheng M, Yu X, Huang H.	Microbiol Spectr	10.1128/spectrum.03238-22	2023
	In Vitro Activity of the Sudapyridine (WX-081) against Non-Tuberculous Mycobacteria Isolated in Beijing, China.	Zhu R, Shang Y, Chen S, Xiao H, Ren R, Wang F, Xue Y, Li L, Li Y, Chu N, Huang H.	Microbiol Spectr	10.1128/spectrum.01372-22	2022
	In vitro and intracellular inhibitory activities of nosiheptide against Mycobacterium abscessus.	Zhu R, Yu X, Zhang T, Kong Y, Wang F, Jia J, Xue Y, Huang H.	Front Microbiol	10.3389/fmicb.2022.926361	2022
Pathogenicity	In Vitro Activities of Bedaquiline and Delamanid against Nontuberculous Mycobacteria Isolated in Beijing, China.	Yu X, Gao X, Li C, Luo J, Wen S, Zhang T, Ma Y, Dong L, Wang F, Huang H.	Antimicrob Agents Chemother	10.1128/aac.00031-19	2019
Phylogeny	A study of the relationship between Nocardia and Mycobacterium diernhoferi--a typical fast growing Mycobacterium.	Stanford JL, Wong JK.	Br J Exp Pathol		1974
Phylogeny	Biochip system for rapid and accurate identification of mycobacterial species from isolates and sputum.	Zhu L, Jiang G, Wang S, Wang C, Li Q, Yu H, Zhou Y, Zhao B, Huang H, Xing W, Mitchelson K, Cheng J, Zhao Y, Guo Y.	J Clin Microbiol	10.1128/jcm.00158-10	2010
Enzymology	Reverse transcription-PCR detection of Mycobacterium leprae in clinical specimens.	Kurabachew M, Wondimu A, Ryon JJ.	J Clin Microbiol	10.1128/jcm.36.5.1352-1356.1998	1998
	Identification and characterization of a 29-kilodalton protein from Mycobacterium tuberculosis culture filtrate recognized by mouse memory effector cells.	Rosenkrands I, Rasmussen PB, Carnio M, Jacobsen S, Theisen M, Andersen P.	Infect Immun	10.1128/iai.66.6.2728-2735.1998	1998
	Growth of group IV mycobacteria on medium containing various saturated and unsaturated fatty acids.	Saito H, Tomioka H, Yoneyama T.	Antimicrob Agents Chemother	10.1128/aac.26.2.164	1984
Metabolism	Purification and characterization of the tween-hydrolyzing esterase of Mycobacterium smegmatis.	Tomioka H.	J Bacteriol	10.1128/jb.155.3.1249-1259.1983	1983
	Compilation of small ribosomal subunit RNA structures.	Neefs JM, Van de Peer Y, De Rijk P, Chapelle S, De Wachter R.	Nucleic Acids Res	10.1093/nar/21.13.3025	1993
Metabolism	Two new Mycobacterium strains and their role in toluene degradation in a contaminated stream.	Tay ST, Hemond HF, Polz MF, Polz MF, Cavanaugh CM, Dejesus I, Krumholz LR.	Appl Environ Microbiol	10.1128/aem.64.5.1715-1720.1998	1998
Pathogenicity	Mechanism of mycobacteriocin and Tween 80 mediated antimycobacterial activity.	Tomioka H, Saito H	J Gen Microbiol	10.1099/00221287-130-12-3085	1984
Metabolism	Characterization of binding sites for a bacteriocin produced by Mycobacterium smegmatis.	Watanabe T, Saito H	J Bacteriol	10.1128/jb.144.1.428-431.1980	1980

References

#11112	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43524
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#36369	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#118931	Collection of Institut Pasteur ; Curators of the CIP; CIP 105384
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Mycobacterium diernhoferi (DSM 43524, ATCC 19340, JCM 6371)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 20NE

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_019456655

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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