Name: Middlebrook 7H9 (MB7H9) Broth Base
Middelbrook 7H9 Broth Base 4.7 g/l
Glycerol 2.0 g/l
Casaminoacids 11.0 g/l
Yeast extract 5.5 g/l
Tween80 (20%) 2.5 g/l
OADC-Enrichment 100 ml
Agar 15.0 g/l
Preparation: Dissolve in Milli-Q-H2O. Before Use add 10% OADC stock solution
Sterilisation: 20 minutes at 121°C
pH before sterilisation:
Maintenance and revitalization
OADC stock solution
old broth-name: OADC-Stock
Bovine Serum Albumin, fraction V 5 %
1% Oleic Acid in 0.2N NaOH 5ml
Glucose 2 %
NaCl 0.85 %
Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this.
Sterilisation: 20 minutes Temperature:121°C
Biological details Culture type:
suitable for: Mycobacteria
Multicenter evaluation of mycobacteria identification by PCR restriction enzyme analysis in laboratories from Latin America and the Caribbean.
Leao SC, Bernardelli A, Cataldi A, Zumarraga M, Robledo J, Realpe T, Mejia GI, da Silva Telles MA, Chimara E, Velazco M, Fernandez J, Rodrigues PA, Guerrero MI, Leon CI, Porras TB, Rastogi N, Goh KS, Suffys P, da Silva Rocha A, dos Santos Netto D, Ritacco V, Lopez B, Barrera L, Palomino JC, Martin A, Portaels F
* These data were automatically processed and therefore are not curated
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