Name: Psychrobacter arcticus 273-4
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 8203

Type strain:

Species: Psychrobacter arcticus

Strain Designation: 273-4

Culture col. no.: DSM 17307, CIP 109905, VKM B-2377

Strain history: <- C. Bakermans, Center for Microbial Ecol. and NASA Astrobiol. Inst., Michigan State Univ., USA; 273-4 <- T. Vishnivetskaya, Dept. Food Sci., North Carolina State Univ., USA

NCBI tax ID(s): 334543 (species)

Section

Psychrobacter arcticus 273-4 is an aerobe, psychrophilic, Gram-negative bacterium that was isolated from permafrost sediment cores.

Gram-negative
ovoid-shaped
aerobe
psychrophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Moraxellaceae*
	Genus	*Psychrobacter*
	Species	**Psychrobacter arcticus**
	Full Scientific Name (PNU)	Psychrobacter arcticus Bakermans et al. 2006

Information on morphological and physiological properties Morphology

[Ref.: #31679]	Gram stain	negative
[Ref.: #31679]	Cell length	1.62 µm
[Ref.: #31679]	Cell width	0.73 µm
[Ref.: #31679]	Cell shape	ovoid-shaped
[Ref.: #31679]	Motility	no

[Ref.: #31679]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6889]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #6889]

Culture medium growth

[Ref.: #6889]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #6889]

Culture medium composition

expand / minimize

[Ref.: #41745]

Culture medium

Marine agar (MA)

[Ref.: #41745]

Culture medium growth

[Ref.: #41745]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #6889]	growth	20 °C
[Ref.: #31679]	optimum	22 °C
[Ref.: #41745]	growth	20 °C

[Ref.: #6889]	Temperature range	psychrophilic
[Ref.: #31679]	Temperature range	psychrophilic
[Ref.: #41745]	Temperature range	psychrophilic

	pH	Kind of pH		pH	pH range
[Ref.: #31679]			optimum	07-09
[Ref.: #31679]			growth	07-09	alkaliphile

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31679]

Oxygen tolerance

aerobe

[Ref.: #31679]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31679]		NaCl		growth	7.25	%
[Ref.: #31679]		NaCl		optimum	7.25	%

[Ref.: #31679]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31679]	acetate ChEBI	+	carbon source
[Ref.: #31679]	glutamate ChEBI	+	carbon source
[Ref.: #31679]	lactate ChEBI	+	carbon source
[Ref.: #31679]	leucine ChEBI	+	carbon source
[Ref.: #31679]	pyruvate ChEBI	+	carbon source

	Enzyme	Enzyme activity	EC number
[Ref.: #31679]	alkaline phosphatase	+	3.1.3.1
[Ref.: #31679]	catalase	+	1.11.1.6
[Ref.: #31679]	cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6889]	Sample type/isolated from	permafrost sediment cores
[Ref.: #6889]	Geographic location (country and/or sea, region)	Siberia, Kolyma lowland
[Ref.: #6889]	Country	Russia
[Ref.: #6889]	Country ISO 3 Code	RUS
[Ref.: #6889]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Sediment
#Environmental	#Terrestrial	#Core sample
#Environmental	#Terrestrial	#Permafrost

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY444822 (>99% sequence identity) for Psychrobacter from Microbeatlas

Aquatic Samples	3187
Soil Samples	925
Animal Samples	3350
Plant Samples	172
Total Samples	7634

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6889]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6889]	Psychrobacter arcticus 273-4 small subunit ribosomal RNA (rrn) gene, partial sequence	AY444822	1504	ENA	259536 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Psychrobacter arcticus 273-4 273-4	GCA_000012305	complete	GenBank	259536 tax ID	*
[Ref.: #66792]	Psychrobacter arcticus 273-4	259536.19	complete	PATRIC	259536 tax ID	*
[Ref.: #66792]	Psychrobacter arcticus 273-4	637000226	complete	JGI IMG/M	259536 tax ID	*
[Ref.: #66792]	Klebsiella pneumoniae TRqt-38	GCA_002751425	scaffold	PATRIC	573 tax ID	*

[Ref.: #31679]

GC-content

42.7 mol%

Availability in culture collections External links

[Ref.: #6889]

Culture collection no.

DSM 17307, CIP 109905, VKM B-2377

[Ref.: #20218]

Associated Passport(s) in StrainInfo

690311, 857673, 690312

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Psychrobacter cryohalolentis sp. nov. and Psychrobacter arcticus sp. nov., isolated from Siberian permafrost.	Bakermans C, Ayala-Del-Rio HL, Ponder MA, Vishnivetskaya T, Gilichinsky D, Thomashow MF, Tiedje JM	Int J Syst Evol Microbiol	10.1099/ijs.0.64043-0	2006	*
Metabolism	Metabolic activity of Siberian permafrost isolates, Psychrobacter arcticus and Exiguobacterium sibiricum, at low water activities.	Ponder MA, Thomashow MF, Tiedje JM	Extremophiles	10.1007/s00792-008-0151-0	2008	*
Metabolism	Psychrobacter arcticus 273-4 uses resource efficiency and molecular motion adaptations for subzero temperature growth.	Bergholz PW, Bakermans C, Tiedje JM	J Bacteriol	10.1128/JB.01377-08	2009	*
Genetics	The genome sequence of Psychrobacter arcticus 273-4, a psychroactive Siberian permafrost bacterium, reveals mechanisms for adaptation to low-temperature growth.	Ayala-del-Rio HL, Chain PS, Grzymski JJ, Ponder MA, Ivanova N, Bergholz PW, Di Bartolo G, Hauser L, Land M, Bakermans C, Rodrigues D, Klappenbach J, Zarka D, Larimer F, Richardson P, Murray A, Thomashow M, Tiedje JM	Appl Environ Microbiol	10.1128/AEM.02101-09	2010	*
Enzymology	Structure and function of the first full-length murein peptide ligase (Mpl) cell wall recycling protein.	Das D, Herve M, Feuerhelm J, Farr CL, Chiu HJ, Elsliger MA, Knuth MW, Klock HE, Miller MD, Godzik A, Lesley SA, Deacon AM, Mengin-Lecreulx D, Wilson IA	PLoS One	10.1371/journal.pone.0017624	2011	*
Biotechnology	Functional expression and characterization of five wax ester synthases in Saccharomyces cerevisiae and their utility for biodiesel production.	Shi S, Valle-Rodriguez JO, Khoomrung S, Siewers V, Nielsen J	Biotechnol Biofuels	10.1186/1754-6834-5-7	2012	*
Metabolism	Toward understanding life under subzero conditions: the significance of exploring psychrophilic "cold-shock" proteins.	Kuhn E	Astrobiology	10.1089/ast.2012.0858	2012	*
Enzymology	Biofilm formation by Psychrobacter arcticus and the role of a large adhesin in attachment to surfaces.	Hinsa-Leasure SM, Koid C, Tiedje JM, Schultzhaus JN	Appl Environ Microbiol	10.1128/AEM.00867-13	2013	*
Enzymology	Branched-chain 2-keto acid decarboxylases derived from Psychrobacter.	Wei J, Timler JG, Knutson CM, Barney BM	FEMS Microbiol Lett	10.1111/1574-6968.12208	2013	*
Metabolism	DNA double-strand break repair at--15{degrees}C.	Dieser M, Battista JR, Christner BC	Appl Environ Microbiol	10.1128/AEM.02845-13	2013	*
Phylogeny	Structural Investigation of the Oligosaccharide Portion Isolated from the Lipooligosaccharide of the Permafrost Psychrophile Psychrobacter arcticus 273-4.	Casillo A, Parrilli E, Filomena S, Lindner B, Lanzetta R, Parrilli M, Tutino ML, Corsaro MM	Mar Drugs	10.3390/md13074539	2015	*
Phylogeny	Psychrobacter glaciei sp. nov., isolated from the ice core of an Arctic glacier.	Zeng YX, Yu Y, Liu Y, Li HR	Int J Syst Evol Microbiol	10.1099/ijsem.0.000939	2016	*
Enzymology	Biochemical characterization of ParI, an orphan C5-DNA methyltransferase from Psychrobacter arcticus 273-4.	Grgic M, Williamson A, Kjaereng Bjerga GE, Altermark B, Leiros I	Protein Expr Purif	10.1016/j.pep.2018.05.012	2018	*
Metabolism	Lipid A structural characterization from the LPS of the Siberian psychro-tolerant Psychrobacter arcticus 273-4 grown at low temperature.	Casillo A, Ziaco M, Lindner B, Parrilli E, Schwudke D, Holgado A, Beyaert R, Lanzetta R, Tutino ML, Corsaro MM	Extremophiles	10.1007/s00792-018-1051-6	2018	*
Enzymology	Cell-wall associated polysaccharide from the psychrotolerant bacterium Psychrobacter arcticus 273-4: isolation, purification and structural elucidation.	Casillo A, Ricciardelli A, Parrilli E, Tutino ML, Corsaro MM	Extremophiles	10.1007/s00792-019-01113-8	2019	*
	Development and use of genetic system to identify genes required for efficient low-temperature growth of Psychrobacter arcticus 273-4.	Bakermans C, Sloup RE, Zarka DG, Tiedje JM, Thomashow MF	Extremophiles	10.1007/s00792-008-0193-3	2008	*
	Physicochemical Approach to Understanding the Structure, Conformation, and Activity of Mannan Polysaccharides.	Casillo A, Fabozzi A, Russo Krauss I, Parrilli E, Biggs CI, Gibson MI, Lanzetta R, Appavou MS, Radulescu A, Tutino ML, Paduano L, Corsaro MM	Biomacromolecules	10.1021/acs.biomac.0c01659	2021	*

References

#6889

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17307

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#31679

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27956 (see below)

#41745 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#27956

IJSEM 1285 2006 ( DOI 10.1099/ijs.0.64043-0 , PubMed 16738105 )

* These data were automatically processed and therefore are not curated

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