Name: Acinetobacter lwoffii DSM 2403
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 8058

Type strain:

Species: Acinetobacter lwoffii

Culture col. no.: DSM 2403, ATCC 15309, CIP 644.10, JCM 6840, NCTC 5866, ATCC 17986, CCM 5581, CCUG 12805, CCUG 33984, CECT 453, CIP 64.10, LMG 1029, LMG 1240, NBRC 109760, NCIMB 12456

Strain history: ATCC 15309 <-- NCTC 5866 <-- A. Lwoff.

NCBI tax ID(s): 981327 (strain), 28090 (species)

Other strains from Acinetobacter lwoffii

A. lwoffii 2A, DSM 101862
A. lwoffii ES1-117-3, DSM 30502
A. lwoffii ES1-189-5, DSM 30543
A. lwoffii ES1-189-6, DSM 30544
A. lwoffii ES1-189-7, DSM 30545
A. lwoffii ES1-230-1c, DSM 30554
A. lwoffii ES1-306b, DSM 30571
A. lwoffii ES2-W2a2, DSM 30617
A. lwoffii ES-12, DSM 30754
A. lwoffii Stev48, DSM 103056
A. lwoffii alk 3PO c), DSM 30932
A. lwoffii alk 4PO c), DSM 30935
A. lwoffii STI05065(IMET), Tb2,
A. lwoffii CIP 110447, NIPH 478
A. lwoffii CIP 102725
A. lwoffii CIP 55.102
A. lwoffii CIP 55.113
A. lwoffii CIP 70.20, ATCC 17985
A. lwoffii CIP 55.112
A. lwoffii CIP 101718
A. lwoffii CIP 54.98
A. lwoffii CIP 100598
A. lwoffii CIP 110448, NIPH 715
A. lwoffii CIP 70.36, ATCC 17987, NCTC 5867
A. lwoffii CIP 102136
A. lwoffii CIP 64.7, ATCC 17910, NCTC 10309
A. lwoffii CIP 70.31, ATCC 9957, CCUG 61662, ...
A. lwoffii CIP 101966
A. lwoffii CCUG 156, ATCC 14291
A. lwoffii CCUG 32185
A. lwoffii CCUG 32443
A. lwoffii CCUG 34253
A. lwoffii CCUG 35742 B
A. lwoffii CCUG 37364
A. lwoffii CCUG 56521
A. lwoffii CCUG 57819
A. lwoffii CCUG 57888
A. lwoffii CCUG 58423
A. lwoffii CCUG 59661
A. lwoffii CCUG 59881
A. lwoffii CCUG 59975
A. lwoffii CCUG 60151
A. lwoffii CCUG 60343
A. lwoffii CCUG 60534
A. lwoffii CCUG 61917
A. lwoffii S2/2 B, DSM 110883
A. lwoffii S 58/4, DSM 110938
A. lwoffii GC2, JCM 31073, DSM 26953

Section

Acinetobacter lwoffii DSM 2403 is a mesophilic bacterium that was isolated from Urine.

mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Moraxellaceae*
	Genus	*Acinetobacter*
	Species	**Acinetobacter lwoffii**
	Full Scientific Name (PNU)	Acinetobacter lwoffii (Audureau 1940) Brisou and Prévot 1954 (Approved Lists 1980)

Synonym

"Moraxella lwoffi"

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.972

[Ref.: #69480]	Motility	no Confidence in %94.518

[Ref.: #1076]

Type of hemolysis

gamma

Information on culture and growth conditions Culture and growth conditions

[Ref.: #41624]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #41624]

Culture medium growth

[Ref.: #41624]

Culture medium composition

expand / minimize

[Ref.: #1076]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #1076]

Culture medium growth

[Ref.: #1076]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #1076]

Culture medium composition

expand / minimize

[Ref.: #1076]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #1076]

Culture medium growth

[Ref.: #1076]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #1076]

Culture medium composition

expand / minimize

[Ref.: #1076]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #1076]

Culture medium growth

[Ref.: #1076]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #1076]

Culture medium composition

expand / minimize

[Ref.: #68368]

Culture medium

MacConkey medium * from API 20E

[Ref.: #68368]

Culture medium growth

* from API 20E

		Temperature
[Ref.: #1076]	growth	30 °C
[Ref.: #41624]	growth	30 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #1076]	Temperature range	mesophilic
[Ref.: #41624]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Test 2

Reference

[Ref.: #1076]
Mueller-Hinton Agar



yes
Inhibition zone diameter in mm
24-26
8
30
34
22
14-16
16
28-30
22
40
32
32
28-30
32
16
24
10
30
30
18
20-22
16
22
18-20
40-42
24
34
26-28
20
18
32
16-18
resistant0
20-22
resistant0
32

[Ref.: #1076]
Mueller-Hinton Agar
30
1
aerob
yes
Inhibition zone diameter in mm
24-26
8
30
34
22
20
16
30
20-22
40-42
30
34-36
26
28-30
14
26
10
32
28
18
20
14
20
18
30
24-26
32-34
28
20
16
30-32
20
resistant0
20
resistant0
26

Only first 15 entries are displayed. Click here to see all.

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.996

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	-	fermentation	* from API 20E
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE, API 20E
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE, API 20E
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68368]	D-mannitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE, API 20E
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68368]	L-arabinose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68368]	melibiose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE, API 20E
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	sucrose ChEBI	-	fermentation	* from API 20E
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE, API 20E
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE, API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite
[Ref.: #68368]	acetoin ChEBI	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	* from API 20E
[Ref.: #68369]	indole ChEBI	* from API 20NE, API 20E
[Ref.: #68368]	nitrite ChEBI	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	-		* from API 20E
[Ref.: #68369]		indole ChEBI		-	* from API 20NE, API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE, API 20E
[Ref.: #68368]	beta-galactosidase	-	3.2.1.23	* from API 20E
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #1076]	catalase	+	1.11.1.6
[Ref.: #68369]	cytochrome oxidase	-	1.9.3.1	* from API 20NE
[Ref.: #1076]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68369]	gelatinase	-		* from API 20NE, API 20E
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE, API 20E
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #1076]	14247	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	+/-	not determinedn.d.	+	not determinedn.d.	not determinedn.d.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #1076]	22089	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	+	-	+	-	+	-
[Ref.: #1076]	20960	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	+	not determinedn.d.
[Ref.: #1076]	20959	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	+	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Urine
* marker position based on {}

[Ref.: #69479]

Global distribution of 16S sequence X81665 (>99% sequence identity) for Acinetobacter from Microbeatlas

Aquatic Samples	2946
Soil Samples	822
Animal Samples	6831
Plant Samples	348
Total Samples	10947

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1076]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #1076]	Acinetobacter lwoffii gene for 16S rRNA, partial sequence, strain: JCM 6840	AB626125	1460	ENA	28090 tax ID
[Ref.: #67770]	A.lwoffii 16S rRNA gene (DSM2403)	X81665	1460	ENA	28090 tax ID
[Ref.: #20218]	Acinetobacter lwoffii 16S rRNA gene, partial	AJ247205	452	ENA	28090 tax ID	*
[Ref.: #20218]	A.lwoffii gene for 16S rRNA	X74894	730	ENA	981327 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Acinetobacter lwoffii NCTC 5866 = CIP 64.10 = NIPH 512 NCTC 5866	GCA_000248355	contig	GenBank	981327 tax ID
[Ref.: #67770]	Acinetobacter lwoffii NBRC 109760	GCA_000836095	contig	GenBank	28090 tax ID
[Ref.: #67770]	Acinetobacter lwoffii NCTC5866	GCA_900699155	contig	GenBank	28090 tax ID
[Ref.: #66792]	Acinetobacter lwoffii NCTC 5866 = CIP 64.10	981327.6	wgs	PATRIC	981327 tax ID	*
[Ref.: #66792]	Acinetobacter lwoffii strain NBRC 109760	28090.13	wgs	PATRIC	28090 tax ID	*
[Ref.: #66792]	Acinetobacter lwoffii strain NCTC5866	28090.96	complete	PATRIC	28090 tax ID	*

[Ref.: #67770]

GC-content

46 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Acinetobacter lwoffii strain NCTC5866 PATRIC: 28090.96
[Ref.: #69480]	gram-positive	no	99.972	yes
[Ref.: #69480]	spore-forming	no	99.996	yes
[Ref.: #69480]	anaerobic	no	99.709	no
[Ref.: #69480]	thermophile	no	99.942	no
[Ref.: #69480]	motile	no	94.518	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Acinetobacter lwoffii strain NCTC5866 PATRIC: 28090.96
[Ref.: #69481]	spore-forming	no	100	no

Availability in culture collections External links

[Ref.: #1076]

Culture collection no.

DSM 2403, ATCC 15309, CIP 644.10, JCM 6840, NCTC 5866, ATCC 17986, CCM 5581, CCUG 12805, CCUG 33984, CECT 453, CIP 64.10, LMG 1029, LMG 1240, NBRC 109760, NCIMB 12456

[Ref.: #20218]

Associated Passport(s) in StrainInfo

1331, 1322, 182457, 1339

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Strain "Acinetobacter mesopotamicus" GC2 Does Not Represent a Novel Species, but Belongs to the Species Acinetobacter lwoffii as Revealed by Whole-Genome Sequence-Based Analysis.	Nemec A	Curr Microbiol	10.1007/s00284-020-02275-x	2020	*
Phylogeny	The status of the genus Prolinoborus (Pot et al. 1992) and the species Prolinoborus fasciculus (Pot et al. 1992).	Glaeser SP, Pulami D, Blom J, Eisenberg T, Goesmann A, Bender J, Wilharm G, Kampfer P	Int J Syst Evol Microbiol	10.1099/ijsem.0.004404	2020	*
Phylogeny	Acinetobacter mesopotamicus sp. nov., Petroleum-degrading Bacterium, Isolated from Petroleum-Contaminated Soil in Diyarbakir, in the Southeast of Turkey.	Acer O, Guven K, Poli A, Di Donato P, Leone L, Buono L, Guven RG, Nicolaus B, Finore I	Curr Microbiol	10.1007/s00284-020-02134-9	2020	*
Phylogeny	Revising the taxonomy of the Acinetobacter lwoffii group: The description of Acinetobacter pseudolwoffii sp. nov. and emended description of Acinetobacter lwoffii.	Nemec A, Radolfova-Krizova L, Maixnerova M, Nemec M, Clermont D, Bzdil J, Jezek P, Spanelova P	Syst Appl Microbiol	10.1016/j.syapm.2018.10.004	2018	*
Phylogeny	Acinetobacter harbinensis sp. nov., isolated from river water.	Li W, Zhang D, Huang X, Qin W	Int J Syst Evol Microbiol	10.1099/ijs.0.055251-0	2014	*
Enzymology	Genes encoding OXA-134-like enzymes are found in Acinetobacter lwoffii and A. schindleri and can be used for identification.	Turton JF, Hyde R, Martin K, Shah J	J Clin Microbiol	10.1128/JCM.06173-11	2012	*
Enzymology	Taxonomic studies of Acinetobacter species based on the electrophoretic analysis of enzymes.	Nishimura Y, Kanzaki H, Iizuka H	J Basic Microbiol	10.1002/jobm.3620280605	1988	*

References

#1076

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2403

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#41624 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Acinetobacter lwoffii strain NCTC5866

Acinetobacter lwoffii strain NCTC5866

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