Micromonospora cremea (DSM 45599, CECT 7891, CR 30)

Name: Micromonospora cremea (DSM 45599, CECT 7891, CR 30)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 8021

Type strain

Strain Designation CR 30, CR30

Culture col. no. DSM 45599 CECT 7891 CR 30 JCM 31700 CR30

NCBI tax ID(s) 709881

Special Collections DSMZ JCM Wink compendium Literature strains

History <- M. E. Trujillo, Universidad de Salamanca, Spain; CR 30 <- L. Carro CECT 7891 <-- M. E. Trujillo, Univ. Salamanca, Spain; CR30.

Links

Micromonospora cremea CR 30 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from rhizosphere of Pisum sativum.

spore-forming Gram-positive aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 382400

CECT 7891,DSM 45599

@ref 20215

Last LPSN update 2026-02-09 11:19:36

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micromonosporales

Family Micromonosporaceae

Genus Micromonospora

Species Micromonospora cremea

Full scientific name Micromonospora cremea Carro et al. 2012

Morphology

Cell morphology

@ref	Gram stain	Cell width	Confidence
30559	positive	0.4 µm
125438	positive		90.567
125439	positive		99.6

Colony morphology

@ref	Colony color	Medium used
69410	Dahlia yellow (1033)	ISP 4
69410	Dahlia yellow (1033)	suter with tyrosine
69410	Beige (1001)	ISP 7
69410	Ivory/ RAL 1007 daffodil yellow (1014)	ISP 5
69410	Ochre yellow (1024)	suter without tyrosine
69410	Pastel yellow (1034)	ISP 3
69410	Sun yellow (1037)	ISP 6
69410	Yellow orange (2000)	ISP 2

Multicellular morphology

@ref	Complex name	Medium name
69410	Aerial mycelium	ISP 2
69410	Aerial mycelium	ISP 3
69410	Aerial mycelium	ISP 4
69410	Aerial mycelium	ISP 5
69410	Aerial mycelium	ISP 6
69410	Aerial mycelium	ISP 7
69410	Aerial mycelium	suter with tyrosine
69410	Aerial mycelium	suter without tyrosine

Pigmentation

@ref	Production	Color	Name
30559
69410		Ral 8001 ochre brown	soluble pigment

Multimedia

@ref:	69410
multimedia content:	DSM_45599_image3.jpeg
multimedia.multimedia content:	DSM_45599_image3.jpeg
caption:	Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)
intellectual property rights:	Helmholtz-Zentrum für Infektionsforschung GmbH
manual_annotation:	1

@ref:	69410
multimedia content:	DSM_45599_image4.jpeg
multimedia.multimedia content:	DSM_45599_image4.jpeg
caption:	Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)
intellectual property rights:	Helmholtz-Zentrum für Infektionsforschung GmbH
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
17740	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
17740	N-Z-AMINE-MEDIUM (DSMZ Medium 554)	Medium recipe at MediaDive	Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
17740	GPHF-MEDIUM (DSMZ Medium 553)	Medium recipe at MediaDive	Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
17740	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
17740	positive	growth	28
30559	positive	growth	10-37
67770	positive	growth	28

Culture pH

@ref	Ability	Type	PH
30559	positive	growth	07-08

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
30559	aerobe
125438	aerobe	90.849
125439	obligate aerobe	99.7

Spore formation

@ref	Spore formation	Confidence
30559
125438		90.248

Halophily

@ref	Salt	Growth	Tested relation	Concentration
30559	NaCl	positive	growth	1 %
69410	NaCl	positive	growth	0 %

Observation

67770

Observationquinones: MK-10(H₄), MK-9(H₄), MK-9(H₆), MK-10(H₆)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
30559	22599 ChEBI	arabinose	+	carbon source
69410	22599 ChEBI	arabinose	+	growth
30559	29016 ChEBI	arginine	+	carbon source
69410	62968 ChEBI	cellulose	-	growth
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	-	hydrolysis	from API Coryne
30559	28757 ChEBI	fructose	+	carbon source
69410	28757 ChEBI	fructose	-	growth
68379	5291 ChEBI	gelatin	+	hydrolysis	from API Coryne
30559	17234 ChEBI	glucose	+	carbon source
69410	17234 ChEBI	glucose	+	growth
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
69410	17268 ChEBI	inositol	+	growth
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
30559	17306 ChEBI	maltose	+	carbon source
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
30559	37684 ChEBI	mannose	+	carbon source
69410	37684 ChEBI	mannose	-	growth
30559	17632 ChEBI	nitrate	+	reduction
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
30559	16634 ChEBI	raffinose	+	carbon source
69410	16634 ChEBI	raffinose	-	growth
69410	26546 ChEBI	rhamnose	+	growth
30559	17814 ChEBI	salicin	+	carbon source
30559	17822 ChEBI	serine	+	carbon source
30559	17992 ChEBI	sucrose	+	carbon source
68379	17992 ChEBI	sucrose	-	fermentation	from API Coryne
69410	17992 ChEBI	sucrose	-	growth
68379	16199 ChEBI	urea	-	hydrolysis	from API Coryne
69410	18222 ChEBI	xylose	-	growth

Enzymes

@ref	Value	Activity	Ec
30559	acid phosphatase	+	3.1.3.2
68382	acid phosphatase	+	3.1.3.2	from API zym
30559	alkaline phosphatase	+	3.1.3.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68379	alkaline phosphatase	+	3.1.3.1	from API Coryne
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68379	beta-glucosidase	-	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
30559	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
30559	cytochrome oxidase	+	1.9.3.1
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
30559	gelatinase	+
68379	gelatinase	+		from API Coryne
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
30559	pyrazinamidase	+	3.5.1.B15
68379	pyrazinamidase	-	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
68382	trypsin	-	3.4.21.4	from API zym
68379	urease	-	3.5.1.5	from API Coryne
68382	valine arylamidase	+		from API zym

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
69410	-	-	-	+	-	+/-	+	+	-	-	+	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
69410	not determinedn.d.	+	+	+	-	+	+	+	-	+/-	+	+	-	+/-	-	+	+/-	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Rhizosphere

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude
17740	rhizosphere of Pisum sativum	Pisum sativum	Zamora province, Canizal (41° 49' 00'' N 6° 13' 00'' W)	Spain	ESP	Europe	41.8167	-6.2167 41.8167/-6.2167
67770	Rhizosphere of a Pisum sativum plant	Pisum sativum	Zamora	Spain	ESP	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
17740	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	IMG-taxon 2630968262 annotated assembly for Micromonospora cremea DSM 45599	contig	GCA_900143515	709881.3	2630968262	709881	75.44

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
17740	Micromonospora cremea partial 16S rRNA gene, type strain CR30T	FN658654	1452		709881

GC content

@ref	GC-content (mol%)	Method
17740	72.4	thermal denaturation, midpoint method (Tm)
30559	72.4
67770	71.47	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Micromonospora cremea DSM 45599
NCBI: GCA_900143515

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	69.90	no
125439	motility	BacteriaNetⓘ	no	83.10	no
125439	gram_stain	BacteriaNetⓘ	positive	99.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Micromonospora cremea strain DSM 45599
PATRIC: 709881.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	90.57	no
125438	anaerobic	anaerobicⓘ	no	95.87	no
125438	spore-forming	spore-formingⓘ	yes	90.25	yes
125438	aerobic	aerobicⓘ	yes	90.85	yes
125438	thermophilic	thermophileⓘ	no	95.50	no
125438	flagellated	motile2+ⓘ	no	88.00	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Diversity of Culturable Bacteria Isolated from Highland Barley Cultivation Soil in Qamdo, Tibet Autonomous Region.	Pan HU, Zhou J, Dawa Z, Dai Y, Zhang Y, Yang H, Wang C, Liu H, Zhou H, Lu X, Tian Y.	Pol J Microbiol	10.33073/pjm-2021-008	2021
Phylogeny	Micromonospora cremea sp. nov. and Micromonospora zamorensis sp. nov., isolated from the rhizosphere of Pisum sativum.	Carro L, Pukall R, Sproer C, Kroppenstedt RM, Trujillo ME	Int J Syst Evol Microbiol	10.1099/ijs.0.038695-0	2012

References

#17740	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45599
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#26890	IJSEM 2971 2012 ( DOI 10.1099/ijs.0.038695-0 , PubMed 22286910 )
#30559	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26890
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69410	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Micromonospora cremea (DSM 45599, CECT 7891, CR 30)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Pigmentation

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Enzymes

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900143515

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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