Micromonospora pisi (DSM 45175, LMG 24546, GUI 15)

Name: Micromonospora pisi (DSM 45175, LMG 24546, GUI 15)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 8015

Type strain

Culture col. no. DSM 45175 LMG 24546 GUI 15 JCM 17025

NCBI tax ID(s) 589240

Special Collections DSMZ JCM Wink compendium Literature strains

History DSMZ <- M. E. Trujillo, Depart. Microbiol. y Gen., Univ. Salamanca, Spain; GUI 15 <- L. Carro JCM <-- LMG 24546 <-- M. E. Trujillo GUI 15.

Links

Micromonospora pisi DSM 45175 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from root nodules of Pisum sativum.

spore-forming Gram-positive aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 368580

DSM 45175,LMG 24546,JCM 17025

@ref 20215

Last LPSN update 2026-05-29 08:26:47

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micromonosporales

Family Micromonosporaceae

Genus Micromonospora

Species Micromonospora pisi

Full scientific name Micromonospora pisi Garcia et al. 2010

Morphology

Cell morphology

@ref	Gram stain	Confidence
29417	positive
125438	positive	92.195
125439		95.143
125439	positive	98.935

Colony morphology

@ref	Colony color	Incubation period	Medium used
21389	Broom yellow (1032)	10-14 days	ISP 2
21389		10-14 days	ISP 3
21389	Ivory (1014)	10-14 days	ISP 4
21389	Light ivory (1015)	10-14 days	ISP 5
21389	Light ivory (1015)	10-14 days	ISP 7
21389	Sand yellow (1002) - Brown beige (8024)	10-14 days	Suter with tyrosine
21389	Ivory (1014)	10-14 days	Suter without tyrosine

Multicellular morphology

@ref	Forms multicellular complex	Medium name
21389		ISP 2
21389		ISP 3
21389		ISP 4
21389		ISP 5
21389		ISP 7
21389		Suter with tyrosine
21389		Suter without tyrosine

Pigmentation

29417

Productionyes

Multimedia

@ref:	15639
multimedia content:	DSM_45175.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_45175.jpg
caption:	Medium 553 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
15639	GPHF-MEDIUM (DSMZ Medium 553)	Medium recipe at MediaDive	Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
21389	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
21389	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
21389	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
21389	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
21389	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
21389	Suter with tyrosine		Name: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes
21389	Suter without tyrosine		Name: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes
15639	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
15639	ISP2 MEDIUM (DSMZ Medium 987)	Medium recipe at MediaDive	Name: ISP 2 MEDIUM (DSMZ Medium 987) Composition: Agar 20.0 g/l Malt extract 10.0 g/l Dextrose 4.0 g/l Yeast extract 4.0 g/l Distilled water
15639	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
15639	positive	growth	28
29417	positive	growth	20-37
29417	positive	optimum	28
67770	positive	growth	28

Culture pH

@ref	Ability	Type	PH	PH range
29417	positive	growth	07-09	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
29417	aerobe
125438	aerobe	92.558
125439	obligate aerobe	98.667

Spore formation

@ref	Spore formation	Confidence
29417
125438		90.726

Halophily

@ref	Salt	Growth	Tested relation	Concentration
21389	NaCl	positive	maximum	7.5 %

Observation

67770

Observationquinones: MK-10(H₄), MK-10(H₆), MK-10(H₈)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
29417	16449 ChEBI	alanine	+	carbon source
21389	22599 ChEBI	arabinose	+
29417	22599 ChEBI	arabinose	+	carbon source
29417	29016 ChEBI	arginine	+	carbon source
29417	17057 ChEBI	cellobiose	+	carbon source
21389	62968 ChEBI	cellulose	+
68379	17634 ChEBI	D-glucose	+	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	+	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
29417	4853 ChEBI	esculin	+	hydrolysis
68379	4853 ChEBI	esculin	+	hydrolysis	from API Coryne
21389	28757 ChEBI	fructose	+
29417	28757 ChEBI	fructose	+	carbon source
29417	28260 ChEBI	galactose	+	carbon source
68379	5291 ChEBI	gelatin	+	hydrolysis	from API Coryne
29417	24265 ChEBI	gluconate	+	carbon source
21389	17234 ChEBI	glucose	+
29417	17234 ChEBI	glucose	+	carbon source
68379	28087 ChEBI	glycogen	+	fermentation	from API Coryne
29417	27570 ChEBI	histidine	+	carbon source
68379	17306 ChEBI	maltose	+	fermentation	from API Coryne
21389	37684 ChEBI	mannose	+
29417	37684 ChEBI	mannose	+	carbon source
29417	28053 ChEBI	melibiose	+	carbon source
21389	17268 ChEBI	myo-inositol	+
68379	17632 ChEBI	nitrate	+	reduction	from API Coryne
29417	15361 ChEBI	pyruvate	+	carbon source
21389	16634 ChEBI	raffinose	+
21389	26546 ChEBI	rhamnose	+
29417	26546 ChEBI	rhamnose	+	carbon source
29417	17814 ChEBI	salicin	+	carbon source
21389	17992 ChEBI	sucrose	+
29417	17992 ChEBI	sucrose	+	carbon source
68379	17992 ChEBI	sucrose	+	fermentation	from API Coryne
68379	16199 ChEBI	urea	+	hydrolysis	from API Coryne
21389	18222 ChEBI	xylose	+
29417	18222 ChEBI	xylose	+	carbon source

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68379	alkaline phosphatase	+	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68379	beta-galactosidase	+	3.2.1.23	from API Coryne
68382	beta-glucosidase	+	3.2.1.21	from API zym
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
29417	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
29417	cytochrome oxidase	+	1.9.3.1
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
29417	gelatinase	+
68379	gelatinase	+		from API Coryne
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
68379	pyrazinamidase	-	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	+	3.4.19.3	from API Coryne
68379	urease	+	3.5.1.5	from API Coryne

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	starch degradation	100	10 of 10
66794	phenylacetate degradation (aerobic)	100	5 of 5
66794	chorismate metabolism	100	9 of 9
66794	1,4-dihydroxy-6-naphthoate biosynthesis	100	6 of 6
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	daunorubicin biosynthesis	100	9 of 9
66794	cardiolipin biosynthesis	100	7 of 7
66794	threonine metabolism	100	10 of 10
66794	ethanol fermentation	100	2 of 2
66794	4-hydroxymandelate degradation	100	9 of 9
66794	palmitate biosynthesis	100	22 of 22
66794	biotin biosynthesis	100	4 of 4
66794	ppGpp biosynthesis	100	4 of 4
66794	methylglyoxal degradation	100	5 of 5
66794	molybdenum cofactor biosynthesis	100	9 of 9
66794	valine metabolism	100	9 of 9
66794	acetate fermentation	100	4 of 4
66794	glycolate and glyoxylate degradation	100	6 of 6
66794	adipate degradation	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	folate polyglutamylation	100	1 of 1
66794	aerobactin biosynthesis	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	taurine degradation	100	1 of 1
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	coenzyme A metabolism	100	4 of 4
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	formaldehyde oxidation	100	3 of 3
66794	ceramide biosynthesis	100	1 of 1
66794	cellulose degradation	100	5 of 5
66794	NAD metabolism	94.44	17 of 18
66794	vitamin B12 metabolism	94.12	32 of 34
66794	phenylalanine metabolism	92.31	12 of 13
66794	leucine metabolism	92.31	12 of 13
66794	Entner Doudoroff pathway	90	9 of 10
66794	serine metabolism	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	gluconeogenesis	87.5	7 of 8
66794	isoleucine metabolism	87.5	7 of 8
66794	peptidoglycan biosynthesis	86.67	13 of 15
66794	flavin biosynthesis	86.67	13 of 15
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	aclacinomycin biosynthesis	85.71	6 of 7
66794	ubiquinone biosynthesis	85.71	6 of 7
66794	heme metabolism	85.71	12 of 14
66794	photosynthesis	85.71	12 of 14
66794	urea cycle	84.62	11 of 13
66794	histidine metabolism	82.76	24 of 29
66794	glycolysis	82.35	14 of 17
66794	proline metabolism	81.82	9 of 11
66794	pentose phosphate pathway	81.82	9 of 11
66794	tryptophan metabolism	81.58	31 of 38
66794	purine metabolism	80.85	76 of 94
66794	glycogen metabolism	80	4 of 5
66794	metabolism of amino sugars and derivatives	80	4 of 5
66794	factor 420 biosynthesis	80	4 of 5
66794	propionate fermentation	80	8 of 10
66794	citric acid cycle	78.57	11 of 14
66794	glutamate and glutamine metabolism	78.57	22 of 28
66794	allantoin degradation	77.78	7 of 9
66794	methionine metabolism	76.92	20 of 26
66794	degradation of sugar acids	76	19 of 25
66794	alanine metabolism	75.86	22 of 29
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	butanoate fermentation	75	3 of 4
66794	sulfopterin metabolism	75	3 of 4
66794	dTDPLrhamnose biosynthesis	75	6 of 8
66794	cyclohexanol degradation	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	non-pathway related	73.68	28 of 38
66794	metabolism of disaccharids	72.73	8 of 11
66794	degradation of hexoses	72.22	13 of 18
66794	degradation of pentoses	71.43	20 of 28
66794	tetrahydrofolate metabolism	71.43	10 of 14
66794	tyrosine metabolism	71.43	10 of 14
66794	lipid metabolism	70.97	22 of 31
66794	arginine metabolism	70.83	17 of 24
66794	myo-inositol biosynthesis	70	7 of 10
66794	oxidative phosphorylation	69.23	63 of 91
66794	vitamin B1 metabolism	69.23	9 of 13
66794	carotenoid biosynthesis	68.18	15 of 22
66794	pyrimidine metabolism	66.67	30 of 45
66794	octane oxidation	66.67	2 of 3
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	lysine metabolism	66.67	28 of 42
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	d-mannose degradation	66.67	6 of 9
66794	cysteine metabolism	66.67	12 of 18
66794	CO2 fixation in Crenarchaeota	66.67	6 of 9
66794	enterobactin biosynthesis	66.67	2 of 3
66794	glutathione metabolism	64.29	9 of 14
66794	d-xylose degradation	63.64	7 of 11
66794	androgen and estrogen metabolism	62.5	10 of 16
66794	degradation of sugar alcohols	62.5	10 of 16
66794	ketogluconate metabolism	62.5	5 of 8
66794	isoprenoid biosynthesis	61.54	16 of 26
66794	phosphatidylethanolamine bioynthesis	61.54	8 of 13
66794	arachidonate biosynthesis	60	3 of 5
66794	hydrogen production	60	3 of 5
66794	lipoate biosynthesis	60	3 of 5
66794	4-hydroxyphenylacetate degradation	60	6 of 10
66794	bile acid biosynthesis, neutral pathway	58.82	10 of 17
66794	degradation of aromatic, nitrogen containing compounds	58.33	7 of 12
66794	propanol degradation	57.14	4 of 7
66794	ascorbate metabolism	54.55	12 of 22
66794	3-phenylpropionate degradation	53.33	8 of 15
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	grixazone biosynthesis	50	1 of 2
66794	chlorophyll metabolism	50	9 of 18
66794	phenol degradation	50	10 of 20
66794	pantothenate biosynthesis	50	3 of 6
66794	kanosamine biosynthesis II	50	1 of 2
66794	coenzyme M biosynthesis	50	5 of 10
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	selenocysteine biosynthesis	50	3 of 6
66794	toluene degradation	50	2 of 4
66794	CMP-KDO biosynthesis	50	2 of 4
66794	lactate fermentation	50	2 of 4
66794	phenylmercury acetate degradation	50	1 of 2
66794	resorcinol degradation	50	1 of 2
66794	polyamine pathway	47.83	11 of 23
66794	phenylpropanoid biosynthesis	46.15	6 of 13
66794	vitamin B6 metabolism	45.45	5 of 11
66794	cholesterol biosynthesis	45.45	5 of 11
66794	nitrate assimilation	44.44	4 of 9
66794	lipid A biosynthesis	44.44	4 of 9
66794	gallate degradation	40	2 of 5
66794	glycine metabolism	40	4 of 10
66794	D-cycloserine biosynthesis	40	2 of 5
66794	bacilysin biosynthesis	40	2 of 5
66794	elloramycin biosynthesis	40	2 of 5
66794	3-chlorocatechol degradation	40	2 of 5
66794	ethylmalonyl-CoA pathway	40	2 of 5
66794	glycine betaine biosynthesis	40	2 of 5
66794	vitamin K metabolism	40	2 of 5
66794	arachidonic acid metabolism	38.89	7 of 18
66794	sulfate reduction	38.46	5 of 13
66794	carnitine metabolism	37.5	3 of 8
66794	cyanate degradation	33.33	1 of 3
66794	sphingosine metabolism	33.33	2 of 6
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	benzoyl-CoA degradation	28.57	2 of 7
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	alginate biosynthesis	25	1 of 4
66794	catecholamine biosynthesis	25	1 of 4
66794	vitamin E metabolism	25	1 of 4

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
21389	+	-	+	+	-	+	+	+/-	+	+	+	not determinedn.d.	+	-	-	+	+	+/-	+	+	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
21389	not determinedn.d.	+	+	-	-	+	+/-	-	+/-	-	+	-	+/-	+	-	+	+	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root nodule

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent
15639	root nodules of Pisum sativum	Pisum sativum	Zamora	Spain	ESP	Europe
67770	Root nodule of Pisum sativum	Pisum sativum	Zamora	Spain	ESP	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
15639	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM363368v1 assembly for Micromonospora pisi DSM 45175	contig	GCA_003633685	589240.3	2758568729	589240	71.17

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
15639	Micromonospora sp. GUI 15 16S rRNA gene, type strain GUI 15	AM944497	1452		589240

GC content

@ref	GC-content (mol%)	Method
15639	71	thermal denaturation, midpoint method (Tm)
29417	71

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM363368v1 assembly for Micromonospora pisi DSM 45175
NCBI: GCA_003633685

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.67	no
125439	gram_stain	BacteriaNetⓘ	positive	98.94	no
125439	motility	BacteriaNetⓘ	no	95.14	no
125439	spore_formation	BacteriaNetⓘ	yes	70.13	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Micromonospora pisi strain DSM 45175
PATRIC: 589240.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	92.20	no
125438	anaerobic	anaerobicⓘ	no	94.87	yes
125438	spore-forming	spore-formingⓘ	yes	90.73	no
125438	aerobic	aerobicⓘ	yes	92.56	yes
125438	thermophilic	thermophileⓘ	no	95.50	no
125438	flagellated	motile2+ⓘ	no	88.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Root Nodule Microsymbionts of Native Coriaria myrtifolia in Algeria.	Gueddou A, Sbissi I, Louati M, Ghodhbane-Gtari F, Cherif-Silini H, Gtari M.	Microbiol Insights	10.1177/11786361221133794	2022
Phylogeny	Micromonospora polyrhachis sp. nov., an actinomycete isolated from edible Chinese black ant (Polyrhachis vicina Roger).	Xiang W, Yu C, Liu C, Zhao J, Yang L, Xie B, Li L, Hong K, Wang X	Int J Syst Evol Microbiol	10.1099/ijs.0.055863-0	2013
Phylogeny	Micromonospora pisi sp. nov., isolated from root nodules of Pisum sativum.	C Garcia L, Martinez-Molina E, Trujillo ME	Int J Syst Evol Microbiol	10.1099/ijs.0.012708-0	2009

References

#15639	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45175
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#21389	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#25824	IJSEM 331 2010 ( DOI 10.1099/ijs.0.012708-0 , PubMed 19651739 )
#29417	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25824
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Micromonospora pisi (DSM 45175, LMG 24546, GUI 15)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Pigmentation

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Enzymes

Pathway annotation

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_003633685

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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