Micromonospora lupini (DSM 44874, LMG 24055, NRRL B-24771)

Name: Micromonospora lupini (DSM 44874, LMG 24055, NRRL B-24771)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 8005

Type strain

Culture col. no. DSM 44874 LMG 24055 NRRL B-24771 Lupac 14N NBRC 106141 1 more

NCBI tax ID(s) 285679

Special Collections DSMZ JCM Wink compendium Literature strains

History <- M. E. Trujillo; Lupac 14N LMG 24055 <-- M. E. Trujillo strain Lupac 14N.

Links

Micromonospora lupini DSM 44874 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from root nodules of Lupinus angustifolius.

spore-forming Gram-positive aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 297448

DSM 44874,LMG 24055,JCM 16031,NBRC 106141

@ref 20215

Last LPSN update 2026-02-09 11:19:36

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micromonosporales

Family Micromonosporaceae

Genus Micromonospora

Species Micromonospora lupini

Full scientific name Micromonospora lupini Trujillo et al. 2007

Synonyms (1)

"Micromonospora lupini"

BacDive ID	Other strains from **Micromonospora lupini** (2)	Type strain
8003	M. lupini DSM 44870, Lupac 08
8004	M. lupini DSM 44873, Lupac 13

Morphology

Cell morphology

32250

Gram stainpositive

Colony morphology

@ref	Colony color	Incubation period	Medium used
20133	Signal orange (2010)	10-14 days	ISP 2
20133	Pastel orange (2003)	10-14 days	ISP 3
20133	Signal orange (2010)	10-14 days	ISP 4
20133		10-14 days	ISP 5
20133	Signal orange (2010)	10-14 days	ISP 6
20133	Signal orange (2010)	10-14 days	ISP 7

Multicellular morphology

@ref	Forms multicellular complex	Medium name
20133		ISP 2
20133		ISP 3
20133		ISP 4
20133		ISP 6
20133		ISP 7

Pigmentation

32250

Productionno

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
12161	N-Z-AMINE-MEDIUM (DSMZ Medium 554)	Medium recipe at MediaDive	Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
12161	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 20.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
20133	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20133	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20133	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20133	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20133	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20133	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

@ref	Growth	Type	Temperature (°C)
12161	positive	growth	28
20133	positive	optimum	28
32250	positive	growth	20-37
32250	positive	optimum	28
67770	positive	growth	28

Physiology and metabolism

Oxygen tolerance

32250

Oxygen toleranceaerobe

Spore formation

32250

Spore formationyes

Halophily

@ref	Salt	Growth	Tested relation	Concentration
32250	NaCl	positive	optimum	1 %

Observation

67770

Observationquinones: MK-10(H₄), MK-10(H₆), MK-9(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
32250	16449 ChEBI	alanine	+	carbon source
20133	22599 ChEBI	arabinose	+
32250	17057 ChEBI	cellobiose	+	carbon source
20133	62968 ChEBI	cellulose	-
32250	4853 ChEBI	esculin	+	hydrolysis
20133	28757 ChEBI	fructose	+
32250	28260 ChEBI	galactose	+	carbon source
20133	17234 ChEBI	glucose	+
32250	17306 ChEBI	maltose	+	carbon source
20133	29864 ChEBI	mannitol	-
32250	29864 ChEBI	mannitol	+	carbon source
32250	37684 ChEBI	mannose	+	carbon source
32250	28053 ChEBI	melibiose	+	carbon source
20133	17268 ChEBI	myo-inositol	-
20133	16634 ChEBI	raffinose	-
32250	16634 ChEBI	raffinose	+	carbon source
20133	26546 ChEBI	rhamnose	-
20133	17992 ChEBI	sucrose	+
32250	17992 ChEBI	sucrose	+	carbon source
32250	27082 ChEBI	trehalose	+	carbon source
20133	18222 ChEBI	xylose	-

Enzymes

@ref	Value	Activity	Ec
32250	acid phosphatase	+	3.1.3.2
68382	acid phosphatase	+	3.1.3.2	from API zym
32250	alkaline phosphatase	+	3.1.3.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
32250	alpha-galactosidase	+	3.2.1.22
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
32250	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
32250	cytochrome oxidase	+	1.9.3.1
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
32250	gelatinase	+
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
32250	pyrazinamidase	+	3.5.1.B15
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
20133	not determinedn.d.	+	+	+	-	+	+	-	-	-	+	+	-	+	-	+	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root nodule

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent
12161	root nodules of Lupinus angustifolius	Lupinus angustifolius	Salamanca	Spain	ESP	Europe
67770	Surface-sterilized nitrogen-fixing nodule of Lupinus angustifolius	Lupinus angustifolius	Salamanca	Spain	ESP	Europe

Taxonmaps

69479

Global distribution of 16S sequence AJ783996 (>99% sequence identity) for Micromonospora from Microbeatlas

Aquatic Samples	161
Soil Samples	1017
Animal Samples	219
Plant Samples	22
Total Samples	1419

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
12161	1	Risk group (German classification) According to TRBA 466
20133	1	German classification According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM4266621v1 assembly for Micromonospora lupini Lupac 14N	scaffold	GCA_042666215	285679	69.4
124043	ASM3953185v1 assembly for Micromonospora lupini JCM 16031	scaffold	GCA_039531855	285679	46.22

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
12161	Micromonospora lupini partial 16S rRNA gene, type strain Lupac 14NT	AJ783996	1457		285679

GC content

@ref	GC-content (mol%)	Method
12161	70.9
67770	70.9	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Induced systemic resistance against Botrytis cinerea by Micromonospora strains isolated from root nodules.	Martinez-Hidalgo P, Garcia JM, Pozo MJ.	Front Microbiol	10.3389/fmicb.2015.00922	2015
Phylogeny	Micromonospora jinlongensis sp. nov., isolated from muddy soil in China and emended description of the genus Micromonospora.	Gao R, Liu C, Zhao J, Jia F, Yu C, Yang L, Wang X, Xiang W.	Antonie Van Leeuwenhoek	10.1007/s10482-013-0074-3	2014
Phylogeny	Micromonospora orduensis sp. nov., isolated from deep marine sediment.	Veyisoglu A, Carro L, Cetin D, Igual JM, Klenk HP, Sahin N	Antonie Van Leeuwenhoek	10.1007/s10482-019-01349-6	2019
Phylogeny	Micromonospora parathelypteridis sp. nov., an endophytic actinomycete with antifungal activity isolated from the root of Parathelypteris beddomei (Bak.) Ching.	Zhao S, Liu C, Zheng W, Ma Z, Cao T, Zhao J, Yan K, Xiang W, Wang X	Int J Syst Evol Microbiol	10.1099/ijsem.0.001614	2017
Phylogeny	Micromonospora zeae sp. nov., a novel endophytic actinomycete isolated from corn root (Zea mays L.).	Shen Y, Zhang Y, Liu C, Wang X, Zhao J, Jia F, Yang L, Yang D, Xiang W	J Antibiot (Tokyo)	10.1038/ja.2014.54	2014
Phylogeny	Micromonospora violae sp. nov., isolated from a root of Viola philippica Car.	Zhang Y, Liu H, Zhang X, Wang S, Liu C, Yu C, Wang X, Xiang W	Antonie Van Leeuwenhoek	10.1007/s10482-014-0184-6	2014
Phylogeny	Micromonospora lupini sp. nov. and Micromonospora saelicesensis sp. nov., isolated from root nodules of Lupinus angustifolius.	Trujillo ME, Kroppenstedt RM, Fernandez-Molinero C, Schumann P, Martinez-Molina E	Int J Syst Evol Microbiol	10.1099/ijs.0.65192-0	2007

References

#12161	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44874
#20133	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#28491	IJSEM 2799 2007 ( DOI 10.1099/ijs.0.65192-0 , PubMed 18048727 )
#32250	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28491
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Micromonospora lupini (DSM 44874, LMG 24055, NRRL B-24771)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_042666215

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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