Micromonospora viridifaciens (DSM 43909, ATCC 31146, CIP 108949)

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

• @ref:	11260
  multimedia content:	DSM_43909-1.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_43909-1.jpg
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

• @ref:	11260
  multimedia content:	DSM_43909.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_43909.jpg
  caption:	Medium 554 28°C
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
11260	N-Z-AMINE-MEDIUM (DSMZ Medium 554)		Medium recipe at MediaDive	Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
11260	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)		Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19977	ISP 2			Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19977	ISP 3			Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19977	ISP 5			Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19977	ISP 6			Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19977	ISP 7			Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
123864	CIP Medium 57		Medium recipe at CIP

Culture temp

Oxygen tolerance

Compound production

Observation

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
19977	22599 ChEBI	arabinose	+/-
19977	62968 ChEBI	cellulose	+/-
19977	28757 ChEBI	fructose	+/-
19977	17234 ChEBI	glucose	+/-
19977	29864 ChEBI	mannitol	+/-
19977	17268 ChEBI	myo-inositol	+/-
123864	17632 ChEBI	nitrate	-	reduction
123864	16301 ChEBI	nitrite	-	reduction
19977	16634 ChEBI	raffinose	+/-
19977	26546 ChEBI	rhamnose	+/-
19977	17992 ChEBI	sucrose	+/-
19977	18222 ChEBI	xylose	+/-

Metabolite production

@ref	Chebi-ID	Metabolite	Production
123864	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
123864	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123864	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
123864	lysine decarboxylase	-	4.1.1.18
123864	ornithine decarboxylase	-	4.1.1.17
123864	oxidase	+
68382	trypsin	+	3.4.21.4	from API zym
123864	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

Isolation source categories

Isolation

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	IMG-taxon 2636416073 annotated assembly for Micromonospora viridifaciens DSM 43909	chromosome	GCA_900091545	1881.4	2636416073	1881	83.84

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
11260	Micromonospora viridifaciens 16S rRNA gene, type strain DSM 43909	X92623	1479		1881

GC content

Predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Metagenomic analysis of soybean endosphere microbiome to reveal signatures of microbes for health and disease.	Chouhan U, Gamad U, Choudhari JK.	J Genet Eng Biotechnol	10.1186/s43141-023-00535-4	2023
	Cloning, expression, and characterization of the Micromonospora viridifaciens neuraminidase gene in Streptomyces lividans.	Sakurada K, Ohta T, Hasegawa M.	J Bacteriol	10.1128/jb.174.21.6896-6903.1992	1992
Metabolism	Structurally homologous sialidases exhibit a commonality in reactivity: Glycoside hydrolase-catalyzed hydrolysis of Kdn-thioglycosides.	Nejatie A, Akintola O, Steves E, Shamsi Kazem Abadi S, Moore MM, Bennet AJ.	Bioorg Chem	10.1016/j.bioorg.2020.104484	2021
Enzymology	Directed evolution of a remarkably efficient Kdnase from a bacterial neuraminidase.	Shamsi Kazem Abadi S, Deen MC, Watson JN, Shidmoossavee FS, Bennet AJ.	Glycobiology	10.1093/glycob/cwz099	2020
Enzymology	Mutants of Micromonospora viridifaciens sialidase have highly variable activities on natural and non-natural substrates.	Jers C, Guo Y, Kepp KP, Mikkelsen JD.	Protein Eng Des Sel	10.1093/protein/gzu054	2015
	Structural and enzymatic characterization of the sialidase SiaPG from Porphyromonas gingivalis.	Dong WB, Jiang YL, Zhu ZL, Zhu J, Li Y, Xia R, Zhou K.	Acta Crystallogr F Struct Biol Commun	10.1107/s2053230x23001735	2023
Enzymology	Neuraminidase substrate promiscuity permits a mutant Micromonospora viridifaciens enzyme to synthesize artificial carbohydrates.	Cheng LL, Shidmoossavee FS, Bennet AJ.	Biochemistry	10.1021/bi500203p	2014
	Structural and functional characterization of a multi-domain GH92 alpha-1,2-mannosidase from Neobacillus novalis.	Kolaczkowski BM, Moroz OV, Blagova E, Davies GJ, Moller MS, Meyer AS, Westh P, Jensen K, Wilson KS, Krogh KBRM.	Acta Crystallogr D Struct Biol	10.1107/s2059798323001663	2023
Pathogenicity	Chemical insight into the emergence of influenza virus strains that are resistant to Relenza.	Shidmoossavee FS, Watson JN, Bennet AJ.	J Am Chem Soc	10.1021/ja405916q	2013
Metabolism	Kinetic and structural evaluation of selected active site mutants of the Aspergillus fumigatus KDNase (sialidase).	Yeung JH, Telford JC, Shidmoossavee FS, Bennet AJ, Taylor GL, Moore MM.	Biochemistry	10.1021/bi401166f	2013
Enzymology	Turnover is rate-limited by deglycosylation for Micromonospora viridifaciens sialidase-catalyzed hydrolyses: conformational implications for the Michaelis complex.	Chan J, Lu A, Bennet AJ.	J Am Chem Soc	10.1021/ja109199p	2011
Enzymology	Bacterial and viral sialidases: contribution of the conserved active site glutamate to catalysis.	Chan J, Watson JN, Lu A, Cerda VC, Borgford TJ, Bennet AJ.	Biochemistry	10.1021/bi201019n	2012
Enzymology	Safe Sialidase Production by the Saprophyte Oerskovia paurometabola: Gene Sequence and Enzyme Purification.	Eneva R, Engibarov S, Gocheva Y, Mitova S, Arsov A, Petrov K, Abrashev R, Lazarkevich I, Petrova P.	Molecules	10.3390/molecules27248922	2022
Metabolism	Brønsted analysis of an enzyme-catalyzed pseudo-deglycosylation reaction: mechanism of desialylation in sialidases.	Shidmoossavee FS, Cheng L, Watson JN, Bennet AJ.	Biochemistry	10.1021/bi100513u	2010
Metabolism	Two nucleophilic mutants of the Micromonospora viridifaciens sialidase operate with retention of configuration by two different mechanisms.	Watson JN, Newstead S, Narine AA, Taylor G, Bennet AJ.	Chembiochem	10.1002/cbic.200500114	2005
Metabolism	Contribution of the active site aspartic acid to catalysis in the bacterial neuraminidase from Micromonospora viridifaciens.	Watson JN, Newstead S, Dookhun V, Taylor G, Bennet AJ.	FEBS Lett	10.1016/j.febslet.2004.10.016	2004
Metabolism	It All Starts with a Sandwich: Identification of Sialidases with Trans-Glycosylation Activity.	Nordvang RT, Nyffenegger C, Holck J, Jers C, Zeuner B, Sundekilde UK, Meyer AS, Mikkelsen JD.	PLoS One	10.1371/journal.pone.0158434	2016
Metabolism	Natural sialoside analogues for the determination of enzymatic rate constants.	Indurugalla D, Watson JN, Bennet AJ.	Org Biomol Chem	10.1039/b613909d	2006
Metabolism	The hydrolase and transferase activity of an inverting mutant sialidase using non-natural beta-sialoside substrates.	Watson JN, Indurugalla D, Cheng LL, Narine AA, Bennet AJ.	Biochemistry	10.1021/bi061489x	2006
Metabolism	Galactose recognition by the carbohydrate-binding module of a bacterial sialidase.	Newstead SL, Watson JN, Bennet AJ, Taylor G.	Acta Crystallogr D Biol Crystallogr	10.1107/s0907444905026132	2005
Metabolism	Structure and role of sialic acids on the surface of Aspergillus fumigatus conidiospores.	Warwas ML, Watson JN, Bennet AJ, Moore MM.	Glycobiology	10.1093/glycob/cwl085	2007
Metabolism	Mechanistic requirements for the efficient enzyme-catalyzed hydrolysis of thiosialosides.	Narine AA, Watson JN, Bennet AJ.	Biochemistry	10.1021/bi0607507	2006
Metabolism	Mismatched hemagglutinin and neuraminidase specificities in recent human H3N2 influenza viruses.	Gulati U, Wu W, Gulati S, Kumari K, Waner JL, Air GM.	Virology	10.1016/j.virol.2005.05.009	2005
Enzymology	Structure and mechanism of action of an inverting mutant sialidase.	Newstead S, Watson JN, Knoll TL, Bennet AJ, Taylor G.	Biochemistry	10.1021/bi050517t	2005
Metabolism	Influence of a mannan binding family 32 carbohydrate binding module on the activity of the appended mannanase.	Mizutani K, Fernandes VO, Karita S, Luis AS, Sakka M, Kimura T, Jackson A, Zhang X, Fontes CM, Gilbert HJ, Sakka K.	Appl Environ Microbiol	10.1128/aem.07457-11	2012
	A glyco-engineering approach for site-specific conjugation to Fab glycans.	Jaramillo ML, Sulea T, Durocher Y, Acchione M, Schur MJ, Robotham A, Kelly JF, Goneau MF, Robert A, Cepero-Donates Y, Gilbert M.	MAbs	10.1080/19420862.2022.2149057	2023
Metabolism	Mutagenesis of the conserved active-site tyrosine changes a retaining sialidase into an inverting sialidase.	Watson JN, Dookhun V, Borgford TJ, Bennet AJ.	Biochemistry	10.1021/bi035396g	2003
	7-Fluorosialyl Glycosides Are Hydrolysis Resistant but Readily Assembled by Sialyltransferases Providing Easy Access to More Metabolically Stable Glycoproteins.	Geissner A, Baumann L, Morley TJ, Wong AKO, Sim L, Rich JR, So PPL, Dullaghan EM, Lessard E, Iqbal U, Moreno M, Wakarchuk WW, Withers SG.	ACS Cent Sci	10.1021/acscentsci.0c01589	2021
	Structure and ligand binding of carbohydrate-binding module CsCBM6-3 reveals similarities with fucose-specific lectins and "galactose-binding" domains.	Boraston AB, Notenboom V, Warren RA, Kilburn DG, Rose DR, Davies G.	J Mol Biol	10.1016/s0022-2836(03)00152-9	2003
Metabolism	Characterization of a Novel cis-3-Hydroxy-l-Proline Dehydratase and a trans-3-Hydroxy-l-Proline Dehydratase from Bacteria.	Watanabe S, Fukumori F, Miyazaki M, Tagami S, Watanabe Y.	J Bacteriol	10.1128/jb.00255-17	2017
Metabolism	Characterization of a phospholipid-regulated beta-galactosidase from Akkermansia muciniphila involved in mucin degradation.	Kosciow K, Deppenmeier U.	Microbiologyopen	10.1002/mbo3.796	2019
	Sialidases and fucosidases of Akkermansia muciniphila are crucial for growth on mucin and nutrient sharing with mucus-associated gut bacteria.	Shuoker B, Pichler MJ, Jin C, Sakanaka H, Wu H, Gascuena AM, Liu J, Nielsen TS, Holgersson J, Nordberg Karlsson E, Juge N, Meier S, Morth JP, Karlsson NG, Abou Hachem M.	Nat Commun	10.1038/s41467-023-37533-6	2023
Enzymology	Crystal structures of aconitase X enzymes from bacteria and archaea provide insights into the molecular evolution of the aconitase superfamily.	Watanabe S, Murase Y, Watanabe Y, Sakurai Y, Tajima K.	Commun Biol	10.1038/s42003-021-02147-5	2021
	Same but not alike: Structure, flexibility and energetics of domains in multi-domain proteins are influenced by the presence of other domains.	Vishwanath S, de Brevern AG, Srinivasan N.	PLoS Comput Biol	10.1371/journal.pcbi.1006008	2018
Enzymology	Crystallization and preliminary crystallographic study of neuraminidase from Micromonospora viridifaciens.	Taylor G, Dineley L, Glowka M, Laver G.	J Mol Biol	10.1016/0022-2836(92)90112-w	1992
	Evaluation of the potential defensive strategy against Influenza A in cell line models.	Antonova E, Glazova O, Gaponova A, Eremyan A, Grebenkina N, Zvereva S, Volkova N, Volchkov P.	F1000Res	10.12688/f1000research.13496.2	2018
Enzymology	Hemagglutinin specificity and neuraminidase coding capacity of neuraminidase-deficient influenza viruses.	Yang P, Bansal A, Liu C, Air GM.	Virology	10.1006/viro.1996.8421	1997
	Red cells bound to influenza virus N9 neuraminidase are not released by the N9 neuraminidase activity.	Air GM, Laver WG.	Virology	10.1006/viro.1995.1401	1995
Genetics	Isolates from Colonic Spirochetosis in Humans Show High Genomic Divergence and Potential Pathogenic Features but Are Not Detected Using Standard Primers for the Human Microbiota.	Thorell K, Inganas L, Backhans A, Agreus L, Ost A, Walker MM, Talley NJ, Kjellstrom L, Andreasson A, Engstrand L.	J Bacteriol	10.1128/jb.00272-19	2019
	Functional and structural analyses reveal that a dual domain sialidase protects bacteria from complement killing through desialylation of complement factors.	Clark ND, Pham C, Kurniyati K, Sze CW, Coleman L, Fu Q, Zhang S, Malkowski MG, Li C.	PLoS Pathog	10.1371/journal.ppat.1011674	2023
Metabolism	Multiple evolutionary origins reflect the importance of sialic acid transporters in the colonization potential of bacterial pathogens and commensals.	Severi E, Rudden M, Bell A, Palmer T, Juge N, Thomas GH.	Microb Genom	10.1099/mgen.0.000614	2021
Enzymology	The three domains of a bacterial sialidase: a beta-propeller, an immunoglobulin module and a galactose-binding jelly-roll.	Gaskell A, Crennell S, Taylor G.	Structure	10.1016/s0969-2126(01)00255-6	1995
Biotechnology	A synbio approach for selection of highly expressed gene variants in Gram-positive bacteria.	Ferro R, Rennig M, Hernandez-Rollan C, Daley DO, Norholm MHH.	Microb Cell Fact	10.1186/s12934-018-0886-y	2018
	Selection and characterization of a neuraminidase-minus mutant of influenza virus and its rescue by cloned neuraminidase genes.	Liu C, Air GM.	Virology	10.1006/viro.1993.1276	1993
Genetics	The structure of a glycoside hydrolase 29 family member from a rumen bacterium reveals unique, dual carbohydrate-binding domains.	Summers EL, Moon CD, Atua R, Arcus VL.	Acta Crystallogr F Struct Biol Commun	10.1107/s2053230x16014072	2016
Enzymology	Structural analysis of a putative family 32 carbohydrate-binding module from the Streptococcus pneumoniae enzyme EndoD.	Abbott DW, Boraston A.	Acta Crystallogr Sect F Struct Biol Cryst Commun	10.1107/s1744309111001874	2011
Enzymology	Crystal structures of respiratory pathogen neuraminidases.	Hsiao YS, Parker D, Ratner AJ, Prince A, Tong L.	Biochem Biophys Res Commun	10.1016/j.bbrc.2009.01.108	2009
	Novel pH-dependent regulation of human cytosolic sialidase 2 (NEU2) activities by siastatin B and structural prediction of NEU2/siastatin B complex.	Rahman MM, Hirokawa T, Tsuji D, Tsukimoto J, Hitaoka S, Yoshida T, Chuman H, Itoh K.	Biochem Biophys Rep	10.1016/j.bbrep.2015.09.017	2015
Metabolism	Enzymatic Synthesis of 6'-Sialyllactose, a Dominant Sialylated Human Milk Oligosaccharide, by a Novel exo-alpha-Sialidase from Bacteroides fragilis NCTC9343.	Guo L, Chen X, Xu L, Xiao M, Lu L.	Appl Environ Microbiol	10.1128/aem.00071-18	2018
Metabolism	Metabolic inhibition of sialyl-Lewis X biosynthesis by 5-thiofucose remodels the cell surface and impairs selectin-mediated cell adhesion.	Zandberg WF, Kumarasamy J, Pinto BM, Vocadlo DJ.	J Biol Chem	10.1074/jbc.m112.403568	2012
Phylogeny	Genome-based classification of micromonosporae with a focus on their biotechnological and ecological potential.	Carro L, Nouioui I, Sangal V, Meier-Kolthoff JP, Trujillo ME, Montero-Calasanz MDC, Sahin N, Smith DL, Kim KE, Peluso P, Deshpande S, Woyke T, Shapiro N, Kyrpides NC, Klenk HP, Goker M, Goodfellow M.	Sci Rep	10.1038/s41598-017-17392-0	2018
	Identification and characterization of NanH2 and NanH3, enzymes responsible for sialidase activity in the vaginal bacterium Gardnerella vaginalis.	Robinson LS, Schwebke J, Lewis WG, Lewis AL.	J Biol Chem	10.1074/jbc.ra118.006221	2019
Pathogenicity	Asteropsin A: an unusual cystine-crosslinked peptide from porifera enhances neuronal Ca2+ influx.	Li H, Bowling JJ, Fronczek FR, Hong J, Jabba SV, Murray TF, Ha NC, Hamann MT, Jung JH.	Biochim Biophys Acta	10.1016/j.bbagen.2012.11.015	2013
Metabolism	A Phytase-Based Reporter System for Identification of Functional Secretion Signals in Bifidobacteria.	Osswald A, Westermann C, Sun Z, Riedel CU.	PLoS One	10.1371/journal.pone.0128802	2015
Metabolism	Structural and biochemical characterization of the Cutibacterium acnes exo-beta-1,4-mannosidase that targets the N-glycan core of host glycoproteins.	Reichenbach T, Kalyani D, Gandini R, Svartstrom O, Aspeborg H, Divne C.	PLoS One	10.1371/journal.pone.0204703	2018
	The structure of the cysteine protease and lectin-like domains of Cwp84, a surface layer-associated protein from Clostridium difficile.	Bradshaw WJ, Kirby JM, Thiyagarajan N, Chambers CJ, Davies AH, Roberts AK, Shone CC, Acharya KR.	Acta Crystallogr D Biol Crystallogr	10.1107/s1399004714009997	2014
Metabolism	Elastin-derived peptides are new regulators of insulin resistance development in mice.	Blaise S, Romier B, Kawecki C, Ghirardi M, Rabenoelina F, Baud S, Duca L, Maurice P, Heinz A, Schmelzer CE, Tarpin M, Martiny L, Garbar C, Dauchez M, Debelle L, Durlach V.	Diabetes	10.2337/db13-0508	2013
Enzymology	Amino Groups of Chitosan Are Crucial for Binding to a Family 32 Carbohydrate Binding Module of a Chitosanase from Paenibacillus elgii.	Das SN, Wagenknecht M, Nareddy PK, Bhuvanachandra B, Niddana R, Balamurugan R, Swamy MJ, Moerschbacher BM, Podile AR.	J Biol Chem	10.1074/jbc.m116.721332	2016
Enzymology	Functional analysis of conserved aromatic amino acids in the discoidin domain of Paenibacillus beta-1,3-glucanase.	Cheng YM, Hsieh FC, Meng M.	Microb Cell Fact	10.1186/1475-2859-8-62	2009
Enzymology	The first identification of carbohydrate binding modules specific to chitosan.	Shinya S, Ohnuma T, Yamashiro R, Kimoto H, Kusaoke H, Anbazhagan P, Juffer AH, Fukamizo T.	J Biol Chem	10.1074/jbc.m113.503243	2013
Metabolism	Bifidobacterium longum subsp. infantis ATCC 15697 alpha-fucosidases are active on fucosylated human milk oligosaccharides.	Sela DA, Garrido D, Lerno L, Wu S, Tan K, Eom HJ, Joachimiak A, Lebrilla CB, Mills DA.	Appl Environ Microbiol	10.1128/aem.06762-11	2012
Metabolism	Fragments of bacterial endoglycosidase s and immunoglobulin g reveal subdomains of each that contribute to deglycosylation.	Dixon EV, Claridge JK, Harvey DJ, Baruah K, Yu X, Vesiljevic S, Mattick S, Pritchard LK, Krishna B, Scanlan CN, Schnell JR, Higgins MK, Zitzmann N, Crispin M.	J Biol Chem	10.1074/jbc.m113.532812	2014
Enzymology	The Aspergillus fumigatus sialidase is a 3-deoxy-D-glycero-D-galacto-2-nonulosonic acid hydrolase (KDNase): structural and mechanistic insights.	Telford JC, Yeung JH, Xu G, Kiefel MJ, Watts AG, Hader S, Chan J, Bennet AJ, Moore MM, Taylor GL.	J Biol Chem	10.1074/jbc.m110.207043	2011
Metabolism	Structure of a SusD homologue, BT1043, involved in mucin O-glycan utilization in a prominent human gut symbiont.	Koropatkin N, Martens EC, Gordon JI, Smith TJ.	Biochemistry	10.1021/bi801942a	2009
Metabolism	Fixation of oligosaccharides to a surface may increase the susceptibility to human parainfluenza virus 1, 2, or 3 hemagglutinin-neuraminidase.	Tappert MM, Smith DF, Air GM.	J Virol	10.1128/jvi.05537-11	2011
Enzymology	The Cell Shape-determining Csd6 Protein from Helicobacter pylori Constitutes a New Family of L,D-Carboxypeptidase.	Kim HS, Im HN, An DR, Yoon JY, Jang JY, Mobashery S, Hesek D, Lee M, Yoo J, Cui M, Choi S, Kim C, Lee NK, Kim SJ, Kim JY, Bang G, Han BW, Lee BI, Yoon HJ, Suh SW.	J Biol Chem	10.1074/jbc.m115.658781	2015
Metabolism	HA-tagging of putative flagellar proteins in Chlamydomonas reinhardtii identifies a novel protein of intraflagellar transport complex B.	Lechtreck KF, Luro S, Awata J, Witman GB.	Cell Motil Cytoskeleton	10.1002/cm.20369	2009
Metabolism	Functional significance of the hemadsorption activity of influenza virus neuraminidase and its alteration in pandemic viruses.	Uhlendorff J, Matrosovich T, Klenk HD, Matrosovich M.	Arch Virol	10.1007/s00705-009-0393-x	2009
Enzymology	Molecular basis of arabinobio-hydrolase activity in phytopathogenic fungi: crystal structure and catalytic mechanism of Fusarium graminearum GH93 exo-alpha-L-arabinanase.	Carapito R, Imberty A, Jeltsch JM, Byrns SC, Tam PH, Lowary TL, Varrot A, Phalip V.	J Biol Chem	10.1074/jbc.m900439200	2009
	Identification of a second Arcanobacterium pyogenes neuraminidase and involvement of neuraminidase activity in host cell adhesion.	Jost BH, Songer JG, Billington SJ.	Infect Immun	10.1128/iai.70.3.1106-1112.2002	2002
Enzymology	Expression, mutagenesis and kinetic analysis of recombinant K1E endosialidase to define the site of proteolytic processing and requirements for catalysis.	Leggate DR, Bryant JM, Redpath MB, Head D, Taylor PW, Luzio JP.	Mol Microbiol	10.1046/j.1365-2958.2002.02908.x	2002
	Proteomics integrated with Escherichia coli vector-based vaccines and antigen microarrays reveals the immunogenicity of a surface sialidase-like protein of Propionibacterium acnes.	Huang CP, Liu YT, Nakatsuji T, Shi Y, Gallo RR, Lin SB, Huang CM.	Proteomics Clin Appl	10.1002/prca.200780103	2008
Metabolism	Heterodimerization of the sialidase NEU1 with the chaperone protective protein/cathepsin A prevents its premature oligomerization.	Bonten EJ, Campos Y, Zaitsev V, Nourse A, Waddell B, Lewis W, Taylor G, d'Azzo A.	J Biol Chem	10.1074/jbc.m109.031419	2009
Enzymology	Cloning, expression, and characterization of a neuraminidase gene from Arcanobacterium pyogenes.	Jost BH, Songer JG, Billington SJ.	Infect Immun	10.1128/iai.69.7.4430-4437.2001	2001
Enzymology	Intra- and extracellular beta-galactosidases from Bifidobacterium bifidum and B. infantis: molecular cloning, heterologous expression, and comparative characterization.	Moller PL, Jorgensen F, Hansen OC, Madsen SM, Stougaard P.	Appl Environ Microbiol	10.1128/aem.67.5.2276-2283.2001	2001
	Improving NMR protein structure quality by Rosetta refinement: a molecular replacement study.	Ramelot TA, Raman S, Kuzin AP, Xiao R, Ma LC, Acton TB, Hunt JF, Montelione GT, Baker D, Kennedy MA.	Proteins	10.1002/prot.22229	2009
Phylogeny	Melissospora conviva gen. nov., sp. nov., a novel actinobacterial genus isolated from beehive through cross-feeding interactions.	Tellatin D, Cornet L, Snauwaert V, Compere P, Ongena M, Quinton L, Stulanovic N, Ribeiro Monteiro S, Rigolet A, Burguet P, Van Damme P, Carro L, Rigali S.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006868	2025
Phylogeny	Micromonospora yasonensis sp. nov., isolated from a Black Sea sediment.	Veyisoglu A, Carro L, Guven K, Cetin D, Sproer C, Schumann P, Klenk HP, Goodfellow M, Sahin N.	Antonie Van Leeuwenhoek	10.1007/s10482-016-0701-x	2016
Phylogeny	Micromonospora equina sp. nov., isolated from soil from a racecourse.	Everest GJ, Meyers PR	Int J Syst Evol Microbiol	10.1099/ijs.0.042929-0	2012
Phylogeny	Eight new species of the genus Micromonospora, Micromonospora citrea sp. nov., Micromonospora echinaurantiaca sp. nov., Micromonospora echinofusca sp. nov. Micromonospora fulviviridis sp. nov., Micromonospora inyonensis sp. nov., Micromonospora peucetia sp. nov., Micromonospora sagamiensis sp. nov., and Micromonospora viridifaciens sp. nov.	Kroppenstedt RM, Mayilraj S, Wink JM, Kallow W, Schumann P, Secondini C, Stackebrandt E	Syst Appl Microbiol	10.1016/j.syapm.2004.12.011	2005