Micromonospora inyonensis (DSM 46123, ATCC 27600, CIP 108951)

Name: Micromonospora inyonensis (DSM 46123, ATCC 27600, CIP 108951)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7993

Type strain

Strain Designation INA

Culture col. no. DSM 46123 ATCC 27600 CIP 108951 IFO 13156 IMET 8306 12 more

NCBI tax ID(s) 47866

Special Collections DSMZ JCM KCTC CIP Wink compendium

Links

Micromonospora inyonensis INA is a mesophilic, Gram-positive, rod-shaped prokaryote that produces antibiotic compounds and was isolated from soil.

antibiotic compound production Gram-positive rod-shaped mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 39080

ATCC 27600,NRRL 3292,DSM 46123,IFO 13156,IMET 8306,JCM 3188,CCRC 13363,NCIMB 12731,HUT 6602,CECT 3295,KCTC 1055,NBRC 13156,BCRC 13363,KACC 20104,CIP 108951

@ref 20215

Last LPSN update 2025-12-16 09:48:43

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Micromonosporales

Family Micromonosporaceae

Genus Micromonospora

Species Micromonospora inyonensis

Full scientific name Micromonospora inyonensis Kroppenstedt et al. 2005

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
120302	positive	rod-shaped
125438			91.5
125438	positive		93.539
125439	positive		99.9
125439			94.5

Colony morphology

@ref	Colony color	Incubation period	Medium used
20191	Yellow olive (6014)	10-14 days	ISP 2
20191	Red green (6013)	10-14 days	ISP 3
20191	Copper brown (8004)	10-14 days	ISP 4
20191	Colorless	10-14 days	ISP 5
20191	Broom yellow (1032)	10-14 days	ISP 6
20191	Dahlia yellow (1033)	10-14 days	ISP 7

Multicellular morphology

@ref	Forms multicellular complex	Medium name
20191		ISP 2
20191		ISP 3
20191		ISP 4
20191		ISP 5
20191		ISP 6
20191		ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
12449	N-Z-AMINE-MEDIUM (DSMZ Medium 554)	Medium recipe at MediaDive	Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
12449	GYM+S MEDIUM (DSMZ Medium 214)	Medium recipe at MediaDive	Name: GYM+S MEDIUM (DSMZ Medium 214) Composition: Starch 20.0 g/l Agar 12.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
20191	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20191	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20191	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20191	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20191	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20191	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
120302	CIP Medium 57	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
12449	positive	growth	28	mesophilic
67770	positive	growth	28	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125438	aerobe	90.772
125439	obligate aerobe	98.4

Spore formation

@ref	Spore formation	Confidence
125439		97.2

Compound production

12449

Compoundsisomycin

Observation

67770

Observationquinones: MK-10(H₄), MK-10(H₆), MK-9(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
20191	22599 ChEBI	arabinose	+/-
20191	62968 ChEBI	cellulose	+/-
20191	28757 ChEBI	fructose	+/-
20191	17234 ChEBI	glucose	+/-
20191	29864 ChEBI	mannitol	+/-
20191	17268 ChEBI	myo-inositol	+/-
120302	17632 ChEBI	nitrate	-	reduction
120302	16301 ChEBI	nitrite	-	reduction
20191	16634 ChEBI	raffinose	+/-
20191	26546 ChEBI	rhamnose	+/-
20191	17992 ChEBI	sucrose	+/-
20191	18222 ChEBI	xylose	+/-

Metabolite production

@ref	Chebi-ID	Metabolite	Production
120302	35581 ChEBI	indole
67770	9169 ChEBI	sisomycin

Enzymes

@ref	Value	Activity	Ec
120302	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
120302	catalase	+	1.11.1.6
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
120302	lysine decarboxylase	-	4.1.1.18
120302	ornithine decarboxylase	-	4.1.1.17
120302	oxidase	-
68382	trypsin	+	3.4.21.4	from API zym
120302	urease	-	3.5.1.5

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
20191	not determinedn.d.	+	+	+	-	+	+	+	+	+	+	+	-	-	-	+	+	+	-	-
120302	-	+	+	+	-	+	-	-	+	-	-	-	-	-	-	+	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
12449	soil		USA	USA	North America
67770	Soil	Inyo National Forest, CA	USA	USA	North America
120302	Environment, Soil		United States of America	USA	North America

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
12449	1	Risk group (German classification) According to TRBA 466
20191	1	German classification According to TRBA 466
120302	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	IMG-taxon 2636415978 annotated assembly for Micromonospora inyonensis DSM 46123	contig	GCA_900091415	47866.4	2636415978	47866	74.48

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
12449	Micromonospora inyonensis 16S rRNA gene, type strain DSM 46123	X92629	1468		47866

GC content

@ref	GC-content (mol%)	Method
67770	71.88	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Micromonospora inyonensis DSM 46123
NCBI: GCA_900091415

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	97.20	no
125439	motility	BacteriaNetⓘ	no	94.50	no
125439	gram_stain	BacteriaNetⓘ	positive	99.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.40	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Micromonospora inyonensis strain DSM 46123
PATRIC: 47866.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	93.54	no
125438	anaerobic	anaerobicⓘ	no	95.83	no
125438	spore-forming	spore-formingⓘ	yes	89.75	no
125438	aerobic	aerobicⓘ	yes	90.77	no
125438	thermophilic	thermophileⓘ	no	91.48	yes
125438	flagellated	motile2+ⓘ	no	91.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Marine actinomycete Streptomyces sp. ISP2-49E, a new source of Rhamnolipid.	Yan X, Sims J, Wang B, Hamann MT.	Biochem Syst Ecol	10.1016/j.bse.2014.03.015	2014
	Evolution-Guided Discovery of Antimycobacterial Triculamin-Like Lasso Peptides.	Merrild A, Svenningsen T, Chevrette MG, Torring T.	Angew Chem Int Ed Engl	10.1002/anie.202425134	2025
	Improving activity of GenB3 and GenB4 in gentamicin dideoxygenation biosynthesis by semi-rational engineering.	Zhai H, Yang L, Ye Q, Kong Z, Pei J, Ji Y, Liu B, Chen X, Tian T, Ni X, Xia H, Zhang S.	Microb Cell Fact	10.1186/s12934-025-02678-0	2025
Genetics	Defining the Species Micromonospora saelicesensis and Micromonospora noduli Under the Framework of Genomics.	Riesco R, Carro L, Roman-Ponce B, Prieto C, Blom J, Klenk HP, Normand P, Trujillo ME.	Front Microbiol	10.3389/fmicb.2018.01360	2018
Enzymology	Uncovering the potential of novel micromonosporae isolated from an extreme hyper-arid Atacama Desert soil.	Carro L, Castro JF, Razmilic V, Nouioui I, Pan C, Igual JM, Jaspars M, Goodfellow M, Bull AT, Asenjo JA, Klenk HP.	Sci Rep	10.1038/s41598-019-38789-z	2019
Phylogeny	Genome-based classification of micromonosporae with a focus on their biotechnological and ecological potential.	Carro L, Nouioui I, Sangal V, Meier-Kolthoff JP, Trujillo ME, Montero-Calasanz MDC, Sahin N, Smith DL, Kim KE, Peluso P, Deshpande S, Woyke T, Shapiro N, Kyrpides NC, Klenk HP, Goker M, Goodfellow M.	Sci Rep	10.1038/s41598-017-17392-0	2018
Phylogeny	Eight new species of the genus Micromonospora, Micromonospora citrea sp. nov., Micromonospora echinaurantiaca sp. nov., Micromonospora echinofusca sp. nov. Micromonospora fulviviridis sp. nov., Micromonospora inyonensis sp. nov., Micromonospora peucetia sp. nov., Micromonospora sagamiensis sp. nov., and Micromonospora viridifaciens sp. nov.	Kroppenstedt RM, Mayilraj S, Wink JM, Kallow W, Schumann P, Secondini C, Stackebrandt E	Syst Appl Microbiol	10.1016/j.syapm.2004.12.011	2005

References

#12449	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 46123
#20191	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#120302	Collection of Institut Pasteur ; Curators of the CIP; CIP 108951
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Micromonospora inyonensis (DSM 46123, ATCC 27600, CIP 108951)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Observation

Metabolite utilization

Metabolite production

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900091415

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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