Micromonospora mirobrigensis (DSM 44830, LMG 22229, NBRC 101890)

Name: Micromonospora mirobrigensis (DSM 44830, LMG 22229, NBRC 101890)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7985

Type strain

Culture col. no. DSM 44830 LMG 22229 NBRC 101890 NRRL B-24769 WA 201 4 more

NCBI tax ID(s) 262898

Special Collections DSMZ JCM CIP Wink compendium Literature strains JMRC

History DSMZ <- M. E. Trujillo, Universidad de Salamanca, Spain; WA201 CIP <- 2005, M.E. Trujillo, Salamanca Univ., Salamanca, Spain: WA201 JCM <-- DSM 44830 <-- M. E. Trujillo WA201. CIP <- 2005, M.E. Trujillo, Salamanca Univ., Salamanca, Spain: WA201

Links

Micromonospora mirobrigensis DSM 44830 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from water.

spore-forming Gram-positive aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 129610

LMG 22229,DSM 44830,JCM 13240,NBRC 101890,CIP 108755

@ref 20215

Last LPSN update 2026-05-29 08:26:46

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micromonosporales

Family Micromonosporaceae

Genus Micromonospora

Species Micromonospora mirobrigensis

Full scientific name Micromonospora mirobrigensis Trujillo et al. 2005

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
31344	positive
120973	positive	rod-shaped
125438	positive		90.944
125439	positive		98.861

Colony morphology

@ref	Colony color	Incubation period	Medium used	Hemolysis ability
20129	Deep orange (2011)	10-14 days	ISP 2
20129	Yellow orange (2000)	10-14 days	ISP 3
20129	Yellow orange (2000)	10-14 days	ISP 6
20129	Yellow orange (2000)	10-14 days	ISP 7
120973				0

Multicellular morphology

@ref	Forms multicellular complex	Medium name
20129		ISP 2
20129		ISP 3
20129		ISP 4
20129		ISP 5
20129		ISP 6
20129		ISP 7

Pigmentation

31344

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
12012	N-Z-AMINE-MEDIUM (DSMZ Medium 554)	Medium recipe at MediaDive	Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
20129	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20129	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20129	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20129	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
36416	MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulum		Distilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
12012	BENNETT'S AGAR (DSMZ Medium 548)	Medium recipe at MediaDive	Name: BENNETT'S AGAR (DSMZ Medium 548) Composition: Agar 15.0 g/l Glucose 10.0 g/l N-Z amine 2.0 g/l Yeast extract 1.0 g/l Beef extract 1.0 g/l Distilled water
12012	GPHF-MEDIUM (DSMZ Medium 553)	Medium recipe at MediaDive	Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
120973	CIP Medium 57	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
12012	positive	growth	28
20129	positive	optimum	28
31344	positive	growth	20-37
31344	positive	optimum	28
36416	positive	growth	30
67770	positive	growth	28
120973	positive	growth	25-41
120973	negative	growth	10
120973	negative	growth	45

Culture pH

@ref	Ability	Type	PH
31344	positive	optimum	7

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
31344	aerobe
120973	obligate aerobe
125439	obligate aerobe	99.775

Spore formation

@ref	Spore formation	Confidence
31344
125438		93.9
120973

Halophily

@ref	Salt	Growth	Tested relation	Concentration
31344	NaCl	positive	growth	<3 %
31344	NaCl	positive	optimum	1.5 %
120973	NaCl	positive	growth	0 %
120973	NaCl		growth	2 %
120973	NaCl		growth	4 %
120973	NaCl		growth	6 %
120973	NaCl		growth	8 %
120973	NaCl		growth	10 %

Observation

67770

Observationquinones: MK-10(H₄), MK-10(H₆), MK-9(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
20129	22599 ChEBI	arabinose	+/-
31344	22599 ChEBI	arabinose	+	carbon source
31344	17057 ChEBI	cellobiose	+	carbon source
20129	62968 ChEBI	cellulose	+/-
120973	16947 ChEBI	citrate	-	carbon source
31344	4853 ChEBI	esculin	+	hydrolysis
120973	4853 ChEBI	esculin	+	hydrolysis
20129	28757 ChEBI	fructose	-
31344	28260 ChEBI	galactose	+	carbon source
20129	17234 ChEBI	glucose	+/-
31344	17234 ChEBI	glucose	+	carbon source
120973	606565 ChEBI	hippurate	+	hydrolysis
31344	27570 ChEBI	histidine	+	carbon source
31344	17306 ChEBI	maltose	+	carbon source
20129	29864 ChEBI	mannitol	+/-
31344	37684 ChEBI	mannose	+	carbon source
31344	28053 ChEBI	melibiose	+	carbon source
20129	17268 ChEBI	myo-inositol	+/-
120973	17632 ChEBI	nitrate	-	reduction
120973	17632 ChEBI	nitrate	-	respiration
120973	16301 ChEBI	nitrite	-	reduction
20129	16634 ChEBI	raffinose	+/-
31344	16634 ChEBI	raffinose	+	carbon source
20129	26546 ChEBI	rhamnose	+/-
20129	17992 ChEBI	sucrose	+/-
31344	17992 ChEBI	sucrose	+	carbon source
31344	27082 ChEBI	trehalose	+	carbon source
20129	18222 ChEBI	xylose	+/-

Metabolite production

@ref	Chebi-ID	Metabolite	Production
120973	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
120973	15688 ChEBI	acetoin	-
120973	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
120973	alcohol dehydrogenase	-	1.1.1.1
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
120973	amylase	+
68382	beta-galactosidase	+	3.2.1.23	from API zym
120973	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
120973	caseinase	+	3.4.21.50
31344	catalase	+	1.11.1.6
120973	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
31344	cytochrome oxidase	+	1.9.3.1
120973	DNase	+
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
120973	gamma-glutamyltransferase	-	2.3.2.2
120973	gelatinase	+
120973	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
120973	lipase	-
68382	lipase (C 14)	-		from API zym
120973	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
120973	ornithine decarboxylase	-	4.1.1.17
120973	oxidase	+
120973	phenylalanine ammonia-lyase	-	4.3.1.24
120973	tryptophan deaminase	-
120973	tween esterase	-
31344	urease	+	3.5.1.5
120973	urease	-	3.5.1.5

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
20129	not determinedn.d.	-	+	+	-	+	-	-	-	-	+	+	+	+	-	+	+	+	-	-
120973	-	+	+	+	-	+	+	-	+	-	+	+	+	+	-	+	+	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Environmental	#Aquatic

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
12012	water	Salamaca, Ciudad Rodrigo	Spain	ESP	Europe
67770	Water of a pond located on a former uranium mine	Ciudad Rodrigo	Spain	ESP	Europe
120973	Environment, Water	Ciudad Rodrigo, Salamanca	Spain	ESP	Europe	2003

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
12012	1	Risk group (German classification) According to TRBA 466
20129	1	German classification According to TRBA 466
120973	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	IMG-taxon 2619619047 annotated assembly for Micromonospora mirobrigensis DSM 44830	scaffold	GCA_900091555	262898.5	2619619047	262898	72.39

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
12012	Micromonospora mirobrigensis 16S rRNA gene, type strain WA201	AJ626950	1511		262898

GC content

@ref	GC-content (mol%)	Method
31344	68.6
67770	68.6	thermal denaturation, midpoint method (Tm)
67770	73.34	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: IMG-taxon 2619619047 annotated assembly for Micromonospora mirobrigensis DSM 44830
NCBI: GCA_900091555

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.78	no
125439	gram_stain	BacteriaNetⓘ	positive	98.86	no
125439	motility	BacteriaNetⓘ	no	88.97	no
125439	spore_formation	BacteriaNetⓘ	yes	68.96	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Micromonospora mirobrigensis strain DSM 44830
PATRIC: 262898.5

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	90.94	no
125438	anaerobic	anaerobicⓘ	no	95.33	yes
125438	spore-forming	spore-formingⓘ	yes	93.90	yes
125438	aerobic	aerobicⓘ	yes	89.37	yes
125438	thermophilic	thermophileⓘ	no	96.00	yes
125438	flagellated	motile2+ⓘ	no	89.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Micromonospora mirobrigensis sp. nov.	Trujillo ME, Fernandez-Molinero C, Velazquez E, Kroppenstedt RM, Schumann P, Mateos PF, Martinez-Molina E	Int J Syst Evol Microbiol	10.1099/ijs.0.63361-0	2005

References

#12012	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44830
#20129	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the JMRC: Jena Microbial Resource Collection (JMRC):
#27658	IJSEM 877 2005 ( DOI 10.1099/ijs.0.63361-0 , PubMed 15774678 )
#31344	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27658
#36416	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#120973	Collection of Institut Pasteur ; Curators of the CIP; CIP 108755
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Micromonospora mirobrigensis (DSM 44830, LMG 22229, NBRC 101890)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900091555

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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