Micromonospora chersina (DSM 44151, ATCC 53710, JCM 9459)

Name: Micromonospora chersina (DSM 44151, ATCC 53710, JCM 9459)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7981

Type strain

Culture col. no. DSM 44151 ATCC 53710 JCM 9459 IFO 15963 IMSNU 22023 1 more

NCBI tax ID(s) 47854

Special Collections DSMZ JCM Wink compendium

Links

Micromonospora chersina DSM 44151 is a mesophilic prokaryote that builds an aerial mycelium and was isolated from soil.

mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 45341

ATCC 53710,DSM 44151,JCM 9459,IFO 15963,NBRC 15963

@ref 20215

Last LPSN update 2025-12-16 09:48:43

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Micromonosporales

Family Micromonosporaceae

Genus Micromonospora

Species Micromonospora chersina

Full scientific name Micromonospora chersina Tomita et al. 1992

Morphology

Cell morphology

@ref	Gram stain	Confidence
125438	positive	92.089
125439	positive	98.6

Colony morphology

@ref	Colony color	Incubation period	Medium used
19635	Orange brown	10-14 days	ISP 2
19635	Orange brown	10-14 days	ISP 3
19635	Orange brown	10-14 days	ISP 4
19635	Orange brown	10-14 days	ISP 5
19635	Signal orange	10-14 days	ISP 6
19635	Orange brown	10-14 days	ISP 7

Multicellular morphology

@ref	Complex name	Complex color	Medium name
19635			ISP 2
19635			ISP 3
19635			ISP 4
19635	Aerial Mycelium	White	ISP 5
19635			ISP 6
19635			ISP 7

Multimedia

@ref:	11551
multimedia content:	DSM_44151.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44151.jpg
caption:	Medium 65 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11551	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19635	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19635	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19635	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19635	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19635	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19635	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
11551	N-Z-AMINE-MEDIUM (DSMZ Medium 554)	Medium recipe at MediaDive	Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
11551	GPHF-MEDIUM (DSMZ Medium 553)	Medium recipe at MediaDive	Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
11551	positive	growth	28	mesophilic
19635	positive	optimum	28	mesophilic
67770	positive	growth	28	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125438	aerobe	90.695
125439	obligate aerobe	99.6

Spore formation

@ref	Spore formation	Confidence
125438		93.046

Halophily

@ref	Salt	Growth	Tested relation	Concentration
19635	NaCl	positive	maximum	2.5 %

Observation

67770

Observationquinones: MK-9(H₄), MK-9(H₆), MK-10(H₄), MK-10(H₆)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
68368	25094 ChEBI	lysine	-	degradation	from API 20E
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
67770		dynemicin
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	-		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68382	beta-galactosidase	+	3.2.1.23	from API zym
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	+	3.2.1.31	from API zym
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68368	gelatinase	+		from API 20E
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
68382	trypsin	+	3.4.21.4	from API zym
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	-	3.5.1.5	from API 20E
68382	valine arylamidase	+		from API zym

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
19635	-	-	-	-	+	-	-	-	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
19635	not determinedn.d.	+	+	+	-	+	+	+	+	+	+	+	+	+	+	+	+	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
11551	soil	Gujarat State	India	IND	Asia
67770	Soil	Gujarat State	India	IND	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11551	1	Risk group (German classification) According to TRBA 466
19635	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	IMG-taxon 2623620611 annotated assembly for Micromonospora chersina DSM 44151	contig	GCA_900091475	47854.6	2623620611	47854	76.66

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
11551	M.chersinia 16S rRNA gene	X92628	1477		47854

GC content

@ref	GC-content (mol%)	Method
67770	72.9	high performance liquid chromatography (HPLC)
67770	73.57	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Micromonospora chersina DSM 44151
NCBI: GCA_900091475

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	60.60	no
125439	motility	BacteriaNetⓘ	no	76.30	no
125439	gram_stain	BacteriaNetⓘ	positive	98.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Micromonospora chersina strain DSM 44151
PATRIC: 47854.6

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	92.09	no
125438	anaerobic	anaerobicⓘ	no	97.35	no
125438	spore-forming	spore-formingⓘ	yes	93.05	no
125438	aerobic	aerobicⓘ	yes	90.70	no
125438	thermophilic	thermophileⓘ	no	96.00	yes
125438	flagellated	motile2+ⓘ	no	87.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	The Impact of Heterologous Regulatory Genes from Lipodepsipeptide Biosynthetic Gene Clusters on the Production of Teicoplanin and A40926.	Zhukrovska K, Binda E, Fedorenko V, Marinelli F, Yushchuk O.	Antibiotics (Basel)	10.3390/antibiotics13020115	2024
	Bioactive Lipodepsipeptides Produced by Bacteria and Fungi.	Evidente A.	Int J Mol Sci	10.3390/ijms232012342	2022
	A discrete intermediate for the biosynthesis of both the enediyne core and the anthraquinone moiety of enediyne natural products.	Bhardwaj M, Cui Z, Daniel Hankore E, Moonschi FH, Saghaeiannejad Esfahani H, Kalkreuter E, Gui C, Yang D, Phillips GN, Thorson JS, Shen B, Van Lanen SG.	Proc Natl Acad Sci U S A	10.1073/pnas.2220468120	2023
Genetics	Genome neighborhood network reveals insights into enediyne biosynthesis and facilitates prediction and prioritization for discovery.	Rudolf JD, Yan X, Shen B.	J Ind Microbiol Biotechnol	10.1007/s10295-015-1671-0	2016
Phylogeny	Rapid PCR amplification of minimal enediyne polyketide synthase cassettes leads to a predictive familial classification model.	Liu W, Ahlert J, Gao Q, Wendt-Pienkowski E, Shen B, Thorson JS.	Proc Natl Acad Sci U S A	10.1073/pnas.2034291100	2003
Metabolism	The crystal structure of DynF from the dynemicin-biosynthesis pathway of Micromonospora chersina.	Kosgei AJ, Miller MD, Bhardwaj M, Xu W, Thorson JS, Van Lanen SG, Phillips GN Jr	Acta Crystallogr F Struct Biol Commun	10.1107/S2053230X21012322	2022
Pathogenicity	[Exploiting bioactive Enediynes from marine microbe based on activity and gene screening].	Pei G, Dai H, Ren B, Liu X, Zhang L	Wei Sheng Wu Xue Bao		2010
Genetics	The biosynthetic genes encoding for the production of the dynemicin enediyne core in Micromonospora chersina ATCC53710.	Gao Q, Thorson JS	FEMS Microbiol Lett	10.1111/j.1574-6968.2008.01112.x	2008
Phylogeny	Micromonospora terminaliae sp. nov., an endophytic actinobacterium isolated from the surface-sterilized stem of the medicinal plant Terminalia mucronata.	Kaewkla O, Thamchaipinet A, Franco CMM	Int J Syst Evol Microbiol	10.1099/ijsem.0.001600	2017
Phylogeny	Cryptoendolithic actinomycetes from antarctic sandstone rock samples: Micromonospora endolithica sp. nov. and two isolates related to Micromonospora coerulea Jensen 1932.	Hirsch P, Mevs U, Kroppenstedt RM, Schumann P, Stackebrandt E	Syst Appl Microbiol	10.1078/072320204322881781	2004

References

#11551	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44151
#19635	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68382	Automatically annotated from API zym .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Micromonospora chersina (DSM 44151, ATCC 53710, JCM 9459)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900091475

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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