Micromonospora coerulea (DSM 43143, ATCC 27008, IFO 13504)

Name: Micromonospora coerulea (DSM 43143, ATCC 27008, IFO 13504)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7889

Type strain

Strain Designation 36

Culture col. no. DSM 43143 ATCC 27008 IFO 13504 KCC A-0175 NBRC 13504 10 more

NCBI tax ID(s) 47856

Special Collections DSMZ JCM KCTC Wink compendium

History <- KCC <- G.M. Luedemann, 36 KCC A-0175 <-- IFO 13504 <-- ATCC 27008 <-- G. M. Luedemann 36.

Links

Micromonospora coerulea 36 is a bacterium that builds an aerial mycelium and was isolated from soil.

genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 92510

ATCC 27008,DSM 43143,IFO 13504,JCM 3175,IMET 8210,NCIMB 12665,VKM Ac-661,CBS 875.71,KCTC 9368,NBRC 13504

@ref 20215

Last LPSN update 2026-02-09 11:19:35

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micromonosporales

Family Micromonosporaceae

Genus Micromonospora

Species Micromonospora coerulea

Full scientific name Micromonospora coerulea Jensen 1932 (Approved Lists 1980)

BacDive ID	Other strains from **Micromonospora coerulea** (3)	Type strain
7890	M. coerulea AA-319, DSM 44396, IFAM AA-319
7891	M. coerulea AA-321, DSM 44397, IFAM AA-321
7892	M. coerulea DSM 46121, ATCC 27359, IMET 8217, INA 510

Morphology

Colony morphology

@ref	Colony color	Incubation period	Medium used
19545	Black red	10-14 days	ISP 2
19545	Fir green	10-14 days	ISP 3
19545	Salmon orange	10-14 days	ISP 4
19545	Fir green	10-14 days	ISP 5
19545		10-14 days	ISP 6
19545	Fir green	10-14 days	ISP 7

Multicellular morphology

@ref	Complex name	Complex color	Medium name
19545			ISP 2
19545			ISP 3
19545			ISP 4
19545	Aerial Mycelium	White	ISP 5
19545			ISP 6
19545			ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
19545	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19545	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19545	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19545	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19545	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19545	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
10814	GYM+S MEDIUM (DSMZ Medium 214)	Medium recipe at MediaDive	Name: GYM+S MEDIUM (DSMZ Medium 214) Composition: Starch 20.0 g/l Agar 12.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
10814	BHI MEDIUM WITH ADDITIONAL GLUCOSE (DSMZ Medium 215b)	Medium recipe at MediaDive	Name: BHI MEDIUM WITH ADDITIONAL GLUCOSE (DSMZ Medium 215b) Composition: Brain heart infusion 37.0 g/l Glucose 5.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
10814	positive	growth	28
19545	positive	optimum	28
67770	positive	growth	28

Physiology and metabolism

Halophily

@ref	Salt	Growth	Tested relation	Concentration
19545	NaCl	positive	maximum	5 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
19545	22599 ChEBI	arabinose	+
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
19545	62968 ChEBI	cellulose	-
68368	16947 ChEBI	citrate	-	assimilation	from API 20E
19545	28757 ChEBI	fructose	+
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
19545	17234 ChEBI	glucose	+
68368	25094 ChEBI	lysine	+	degradation	from API 20E
19545	29864 ChEBI	mannitol	+
19545	17268 ChEBI	myo-inositol	+
68368	18257 ChEBI	ornithine	+	degradation	from API 20E
19545	16634 ChEBI	raffinose	+
19545	26546 ChEBI	rhamnose	+
19545	17992 ChEBI	sucrose	+
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E
19545	18222 ChEBI	xylose	+

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	+		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68368	gelatinase	+		from API 20E
68368	lysine decarboxylase	+	4.1.1.18	from API 20E
68368	ornithine decarboxylase	+	4.1.1.17	from API 20E
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	-	3.5.1.5	from API 20E

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
19545	+	+	+	+	-	-	-	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
10814	soil	Hawaii	USA	USA	North America
67770	Soil, Mt. Heleakala	Maui Island, HI	USA	USA	North America

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
19545	1	Risk group (German classification) According to TRBA 466
10814	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM3954173v1 assembly for Micromonospora coerulea JCM 3175	scaffold	GCA_039541735	47856	44.14

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
10814	M.coerulea 16S rRNA gene	X92598	1476		47856

GC content

@ref	GC-content (mol%)	Method
67770	71.7	high performance liquid chromatography (HPLC)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Examining the fish microbiome: vertebrate-derived bacteria as an environmental niche for the discovery of unique marine natural products.	Sanchez LM, Wong WR, Riener RM, Schulze CJ, Linington RG.	PLoS One	10.1371/journal.pone.0035398	2012
Genetics	Genome-based reclassification of Micromonospora veneta Kaewkla et al. 2022 as a later heterotypic synonym of Micromonospora coerulea Jensen 1932 (Approved lists 1980).	Zhang X, Chen X, Wu X, Liu Y, Wei Y.	Sci Rep	10.1038/s41598-025-13676-y	2025
Pathogenicity	Isolation, antifungal activity, and structure elucidation of the glutarimide antibiotic, streptimidone, produced by Micromonospora coerulea.	Kim BS, Moon SS, Hwang BK.	J Agric Food Chem	10.1021/jf981259s	1999
Phylogeny	Intrageneric relationships among Micromonospora species deduced from gyrB-based phylogeny and DNA relatedness.	Kasai H, Tamura T, Harayama S.	Int J Syst Evol Microbiol	10.1099/00207713-50-1-127	2000
Metabolism	Intraspecific variation of unusual phospholipids from Corynebacterium spp. containing a novel fatty acid.	Niepel T, Meyer H, Wray V, Abraham WR.	J Bacteriol	10.1128/jb.180.17.4650-4657.1998	1998
Phylogeny	Micromonospora rubida sp. nov., a novel actinobacterium isolated from soil of Harbin.	Sun X, Qiu S, Luo X, Jin P, Zhao J, Wu X, Yang J, Wang X, Song J, Xiang W	Antonie Van Leeuwenhoek	10.1007/s10482-021-01550-6	2021
Phylogeny	Micromonospora kangleipakensis sp. nov., isolated from a sample of limestone quarry.	Nimaichand S, Zhang YG, Cheng J, Li L, Zhang DF, Zhou EM, Dong L, Ningthoujam DS, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.052746-0	2013
Phylogeny	Cryptoendolithic actinomycetes from antarctic sandstone rock samples: Micromonospora endolithica sp. nov. and two isolates related to Micromonospora coerulea Jensen 1932.	Hirsch P, Mevs U, Kroppenstedt RM, Schumann P, Stackebrandt E	Syst Appl Microbiol	10.1078/072320204322881781	2004

References

#10814	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43143
#19545	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Micromonospora coerulea (DSM 43143, ATCC 27008, IFO 13504)

Name and taxonomic classification

Morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_039541735

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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