Name: Hydrogenibacillus schlegelii MA-48
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 782

Type strain:

Species: Hydrogenibacillus schlegelii

Strain Designation: MA-48

Culture col. no.: DSM 2000, ATCC 43741, CCUG 26017, CIP 106933, LMG 7133

Strain history: CIP <- 2002, CCUG, Bacillus schlegelii <- 1990, LMG <- DSMZ <- M. Aragno: strain MA-48

NCBI tax ID(s): 1484 (species)

SI-ID 1177

LMG 7133, DSM 2000, ATCC 43741, CCUG 26017, NCIMB 13107, CIP 106933, KCTC3900, VTT E-87279

Other strains from Hydrogenibacillus schlegelii

H. schlegelii MA-51, DSM 2001
H. schlegelii Nr. 71 OMT1, DSM 9129
H. schlegelii Nr. 73 OMT4, DSM 9131
H. schlegelii Nr. 74 OMT7, DSM 9132

Section

Hydrogenibacillus schlegelii MA-48 is a facultative anaerobe, spore-forming, thermophilic bacterium that was isolated from mud surface, eutrophic, holomictic lake.

Gram-variable
rod-shaped
spore-forming
facultative anaerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Hydrogenibacillus*
	Species	**Hydrogenibacillus schlegelii**
	Full Scientific Name (LPSN)	Hydrogenibacillus schlegelii (Schenk and Aragno 1981) Kämpfer et al. 2013

Synonym

Bacillus schlegelii

Information on morphological and physiological properties Morphology

[Ref.: #30816]	Gram stain	variable
[Ref.: #122507]	Gram stain	negative
[Ref.: #69480]	Gram stain	positive Confidence in %97.768

[Ref.: #30816]	Cell length	3.75 µm

[Ref.: #30816]	Cell width	0.6 µm

[Ref.: #30816]	Cell shape	rod-shaped
[Ref.: #122507]	Cell shape	rod-shaped

[Ref.: #122507]	Motility	no

[Ref.: #30816]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #948]

Culture medium

MEDIUM FOR CHEMOLITHOTROPHIC GROWTH OF BACILLUS SCHLEGELII (DSMZ Medium 261)

[Ref.: #948]

Culture medium growth

[Ref.: #948]

Culture medium link

Medium recipe at MediaDive

[Ref.: #948]

Culture medium composition

expand / minimize

[Ref.: #948]

Culture medium

BACILLUS SCHLEGELII HETEROTROPHIC MEDIUM (DSMZ Medium 260)

[Ref.: #948]

Culture medium growth

[Ref.: #948]

Culture medium link

Medium recipe at MediaDive

[Ref.: #948]

Culture medium composition

expand / minimize

[Ref.: #37319]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37319]

Culture medium growth

[Ref.: #37319]

Culture medium composition

expand / minimize

[Ref.: #122507]

Culture medium

CIP Medium 72

[Ref.: #122507]

Culture medium growth

[Ref.: #122507]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #948]		growth	65 °C
[Ref.: #30816]		growth	38-79 °C
[Ref.: #30816]		optimum	72.5 °C
[Ref.: #37319]		growth	65 °C
[Ref.: #48341]		growth	°C
[Ref.: #48341]		growth	65 °C
[Ref.: #122507]	no	growth	45 °C
[Ref.: #122507]	no	growth	37 °C
[Ref.: #122507]	no	growth	30 °C
[Ref.: #122507]	no	growth	22 °C
[Ref.: #122507]	no	growth	10 °C
[Ref.: #122507]	no	growth	55 °C

[Ref.: #948]	Temperature range	thermophilic
[Ref.: #30816]	Temperature range	thermophilic
[Ref.: #37319]	Temperature range	thermophilic
[Ref.: #48341]	Temperature range	thermophilic
[Ref.: #122507]	Temperature range	thermophilic
[Ref.: #122507]	Temperature range	mesophilic
[Ref.: #122507]	Temperature range	psychrophilic
[Ref.: #122507]	Temperature range	thermophilic
[Ref.: #69480]	Temperature range	thermophilic Confidence in %99.999

	pH	Kind of pH		pH
[Ref.: #30816]			optimum	6.5
[Ref.: #30816]			growth	4.2-7.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30816]

Oxygen tolerance

facultative anaerobe

[Ref.: #30816]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

[Ref.: #948]

Name of produced compound

hydrogenase

	Salt	Tested relation		Salt conc.
[Ref.: #30816]	NaCl		optimum	3	%
[Ref.: #122507]	NaCl	no	growth	0	%
[Ref.: #122507]	NaCl	no	growth	2	%
[Ref.: #122507]	NaCl	no	growth	4	%
[Ref.: #122507]	NaCl	no	growth	6	%
[Ref.: #122507]	NaCl	no	growth	8	%
[Ref.: #122507]	NaCl	no	growth	10	%

[Ref.: #30816]

Observation

aggregates in clumps

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #122507]	esculin ChEBI	+	hydrolysis
[Ref.: #122507]	hippurate ChEBI	+	hydrolysis
[Ref.: #30816]	nitrate ChEBI	+	reduction
[Ref.: #122507]	nitrate ChEBI	+	reduction
[Ref.: #122507]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #122507]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #122507]		acetoin ChEBI	-
[Ref.: #122507]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #122507]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #122507]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #30816]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #30816]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #122507]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #122507]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #122507]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #122507]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #122507]	tryptophan deaminase	-
[Ref.: #122507]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122507]	9920	-	-	+	-	-	-	-	-	-	-	-	+	-	-	-	-	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #948]	Sample type/isolated from	mud surface, eutrophic, holomictic lake
[Ref.: #948]	Country	Switzerland
[Ref.: #948]	Country ISO 3 Code	CHE
[Ref.: #948]	Continent	Europe

[Ref.: #48341]	Sample type/isolated from	Eutrophic, holomictic lake, mud surface
[Ref.: #48341]	Country	Switzerland
[Ref.: #48341]	Country ISO 3 Code	CHE
[Ref.: #48341]	Continent	Europe

[Ref.: #122507]	Sample type/isolated from	Environment, Mud surface
[Ref.: #122507]	Country	Switzerland
[Ref.: #122507]	Country ISO 3 Code	CHE
[Ref.: #122507]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Lake (large)
#Environmental	#Terrestrial	#Mud (Sludge)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB042060 (>99% sequence identity) for Hydrogenibacillus schlegelii subclade from Microbeatlas

Aquatic Samples	235
Soil Samples	213
Animal Samples	556
Plant Samples	19
Total Samples	1023

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #948]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122507]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #948]	Bacillus schlegelii gene for 16S ribosomal RNA, partial sequence	AB042060	1515	ENA	1484 tax ID
[Ref.: #20218]	B.schlegelii gene for 16S ribosomal RNA	Z26934	1432	ENA	1484 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Hydrogenibacillus schlegelii DSM 2000	GCA_001543875	scaffold	GenBank	1484 tax ID	*
[Ref.: #66792]	Hydrogenibacillus schlegelii strain DSM 2000	1484.13	wgs	PATRIC	1484 tax ID	*
[Ref.: #66792]	Hydrogenibacillus schlegelii strain MA 48	1484.14	wgs	PATRIC	1484 tax ID	*
[Ref.: #66792]	Hydrogenibacillus schlegelii DSM 2000	2675903597	draft	JGI IMG/M	1484 tax ID	*

[Ref.: #948]	GC-content	66.3 mol%	Buoyant density centrifugation (BD)
[Ref.: #948]	GC-content	64.6 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #30816]	GC-content	67.5 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Hydrogenibacillus schlegelii DSM 2000 NCBI: GCA_001543875.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Hydrogenibacillus schlegelii DSM 2000 NCBI: GCA_001543875.1
[Ref.: #69480]	flagellated	no	73.047	no
[Ref.: #69480]	gram-positive	yes	67.605	no
[Ref.: #69480]	anaerobic	no	80.916	no
[Ref.: #69480]	halophile	no	91.572	no
[Ref.: #69480]	spore-forming	yes	78.108	no
[Ref.: #69480]	thermophile	yes	100	yes
[Ref.: #69480]	glucose-util	yes	78.48	no
[Ref.: #69480]	motile	yes	85.227	no
[Ref.: #69480]	aerobic	no	66.601	yes
[Ref.: #69480]	glucose-ferment	no	90.736	no

Availability in culture collections External links

[Ref.: #948]

Culture collection no.

DSM 2000, ATCC 43741, CCUG 26017, CIP 106933, LMG 7133

[Ref.: #70456]

SI-ID 1177

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Transfer of Bacillus schlegelii to a novel genus and proposal of Hydrogenibacillus schlegelii gen. nov., comb. nov.	Kampfer P, Glaeser SP, Busse HJ	Int J Syst Evol Microbiol	10.1099/ijs.0.045146-0	2012	*

References

#948	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2000

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#30816

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27147 (see below)

#37319 ; Curators of the CIP;
#48341 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 26017

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70456

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID1177.1 )

#122507 Collection of Institut Pasteur ; Curators of the CIP; CIP 106933

#27147

IJSEM 1723 2013 ( DOI 10.1099/ijs.0.045146-0 , PubMed 22922537 )

* These data were automatically processed and therefore are not curated

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Hydrogenibacillus schlegelii DSM 2000

Hydrogenibacillus schlegelii DSM 2000

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