Actinoplanes campanulatus (DSM 43148, ATCC 15348, CBS 190.64)

Name: Actinoplanes campanulatus (DSM 43148, ATCC 15348, CBS 190.64)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7801

Type strain

Strain Designation UNCC 65

Culture col. no. DSM 43148 ATCC 15348 CBS 190.64 IFO 12511 KCC A-0059 13 more

NCBI tax ID(s) 113559

Special Collections DSMZ JCM KCTC Wink compendium

History <- KCC <- CBS <- J.N. Couch, UNCC, 65 KCC A-0059 <-- CBS 190.64 <-- J. N. Couch UNCC 65.

Links

Actinoplanes campanulatus UNCC 65 is a spore-forming, mesophilic prokaryote that was isolated from soil.

spore-forming mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 50190

MTCC 1981,DSM 43148,ATCC 15348,IFO 12511,JCM 3059,CCRC 12545,IMET 9244,NCIMB 12889,NRRL B-3344,VKM Ac-1319,KCTC 9589,CBS 190.64,CECT 3287,NBRC 12511,BCRC 12545

@ref 20215

Last LPSN update 2026-02-09 11:19:33

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micromonosporales

Family Micromonosporaceae

Genus Actinoplanes

Species Actinoplanes campanulatus

Full scientific name Actinoplanes campanulatus (Couch 1963) Stackebrandt and Kroppenstedt 1988

Synonyms (3)

"Ampullaria campanulata"
Ampullariella campanulata
Actinoplanes capillaceus

BacDive ID	Other strains from **Actinoplanes campanulatus** (1)	Type strain
128608	A. campanulatus STH00304(ZIMET), HKI 0304

Morphology

Cell morphology

@ref	Gram stain	Confidence
125438	positive	90.647
125439		93.1
125439	positive	99.9

Colony morphology

@ref	Colony color	Incubation period	Medium used
19940	Pastel yellow (1034)	10-14 days	ISP 2
19940	Pastel yellow (1034)	10-14 days	ISP 3
19940	Pastel yellow (1034)	10-14 days	ISP 4
19940	Pastel yellow (1034)	10-14 days	ISP 5
19940	Pastel yellow (1034)	10-14 days	ISP 6
19940	Pastel yellow (1034)	10-14 days	ISP 7

Multicellular morphology

@ref	Forms multicellular complex	Medium name
19940		ISP 2
19940		ISP 3
19940		ISP 4
19940		ISP 5
19940		ISP 6
19940		ISP 7

Multimedia

@ref:	10818
multimedia content:	DSM_43148-1.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_43148-1.jpg
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

@ref:	10818
multimedia content:	DSM_43148.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_43148.jpg
caption:	Medium 553 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
19940	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19940	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19940	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19940	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19940	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19940	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
10818	GPHF-MEDIUM (DSMZ Medium 553)	Medium recipe at MediaDive	Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
10818	CZAPEK PEPTONE AGAR (DSMZ Medium 83)	Medium recipe at MediaDive	Name: CZAPEK PEPTONE AGAR (DSMZ Medium 83) Composition: Sucrose 30.0 g/l Agar 20.0 g/l Peptone 5.0 g/l NaNO3 3.0 g/l Yeast extract 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 0.5 g/l KCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
10818	positive	growth	28	mesophilic
19940	positive	optimum	28	mesophilic
67770	positive	growth	28	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	98.9

Spore formation

@ref	Spore description	Spore formation	Confidence
19940	Formation of sporangia, spore surface smooth
125439			94.8

Halophily

@ref	Salt	Growth	Tested relation	Concentration
19940	NaCl	positive	growth	0 %

Observation

67770

Observationquinones: MK-9(H₄), MK-10(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
19940	22599 ChEBI	arabinose	-
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
19940	62968 ChEBI	cellulose	-
68368	16947 ChEBI	citrate	-	assimilation	from API 20E
19940	28757 ChEBI	fructose	-
68368	5291 ChEBI	gelatin	-	hydrolysis	from API 20E
19940	17234 ChEBI	glucose	+
68368	25094 ChEBI	lysine	+	degradation	from API 20E
19940	29864 ChEBI	mannitol	-
19940	17268 ChEBI	myo-inositol	-
68368	18257 ChEBI	ornithine	+	degradation	from API 20E
19940	16634 ChEBI	raffinose	-
19940	26546 ChEBI	rhamnose	+
19940	17992 ChEBI	sucrose	+
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E
19940	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	+		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68368	gelatinase	-		from API 20E
68368	lysine decarboxylase	+	4.1.1.18	from API 20E
68368	ornithine decarboxylase	+	4.1.1.17	from API 20E
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	+	3.5.1.5	from API 20E

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
19940	-	+	+	+	-	-	+	-	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
10818	soil
67770	Soil	Douglas, KS	USA	USA	North America

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
19940	1	German classification According to TRBA 466
10818	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1419193v1 assembly for Actinoplanes campanulatus CECT 3287	scaffold	GCA_014191935	113559.4	2824317532	113559	61.53
66792	ASM1464779v1 assembly for Actinoplanes campanulatus JCM 3059	contig	GCA_014647795	113559.5	8068324054	113559	53.08
66792	ASM1686203v1 assembly for Actinoplanes campanulatus NBRC 12511	contig	GCA_016862035	113559.6	8068333511	113559	47.26

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Actinoplanes campanulatus gene for 16S rRNA, partial sequence	AB036995	1472		113559
124043	Actinoplanes campanulatus strain JCM 3059 16S ribosomal RNA gene, partial sequence.	MT760393	972		113559

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Actinoplanes campanulatus CECT 3287
NCBI: GCA_014191935

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.90	no
125439	gram_stain	BacteriaNetⓘ	positive	99.90	no
125439	motility	BacteriaNetⓘ	no	93.10	no
125439	spore_formation	BacteriaNetⓘ	yes	94.80	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Actinoplanes campanulatus CECT 3287
NCBI: GCA_014191935.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	90.65	no
125438	anaerobic	anaerobicⓘ	no	88.32	no
125438	aerobic	aerobicⓘ	yes	87.86	no
125438	spore-forming	spore-formingⓘ	yes	85.66	yes
125438	thermophilic	thermophileⓘ	no	94.75	yes
125438	flagellated	motile2+ⓘ	no	61.33	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Performance of three endophytic actinomycetes in relation to plant growth promotion and biological control of Pythium aphanidermatum, a pathogen of cucumber under commercial field production conditions in the United Arab Emirates	El-Tarabily KA, St. J. Hardy GE, Sivasithamparam K.	Eur J Plant Pathol	10.1007/s10658-010-9689-7	2010
	Plant growth promotion and biological control of Pythium aphanidermatum, a pathogen of cucumber, by endophytic actinomycetes.	El-Tarabily KA, Nassar AH, Hardy GE, Sivasithamparam K.	J Appl Microbiol	10.1111/j.1365-2672.2008.03926.x	2009
	Plant Growth Promoting and Biocontrol Activity of Streptomyces spp. as Endophytes.	Vurukonda SSKP, Giovanardi D, Stefani E.	Int J Mol Sci	10.3390/ijms19040952	2018
	Biocontrol Potential of Endophytic Actinobacteria against Fusarium solani, the Causal Agent of Sudden Decline Syndrome on Date Palm in the UAE.	Alblooshi AA, Purayil GP, Saeed EE, Ramadan GA, Tariq S, Altaee AS, El-Tarabily KA, AbuQamar SF.	J Fungi (Basel)	10.3390/jof8010008	2021
	Aspergillus terreus Inhibits Growth and Induces Morphological Abnormalities in Pythium aphanidermatum and Suppresses Pythium-Induced Damping-Off of Cucumber.	Halo BA, Al-Yahyai RA, Al-Sadi AM.	Front Microbiol	10.3389/fmicb.2018.00095	2018
	Biological Control of Mango Dieback Disease Caused by Lasiodiplodia theobromae Using Streptomycete and Non-streptomycete Actinobacteria in the United Arab Emirates.	Kamil FH, Saeed EE, El-Tarabily KA, AbuQamar SF.	Front Microbiol	10.3389/fmicb.2018.00829	2018
Phylogeny	Actinoplanes sichuanensis sp. nov. and Actinoplanes xinjiangensis sp. nov.	Sun W, Dong GX, Zhang YQ, Wei YZ, Li QP, Yu LY, Klenk HP, Zhang YQ.	Int J Syst Evol Microbiol	10.1099/ijs.0.010561-0	2009
Phylogeny	Actinoplanes flavus sp. nov., a novel cellulase-producing actinobacterium isolated from coconut palm rhizosphere soil.	Luo X, Sun X, Huang Z, He C, Zhao J, Xiang W, Song J, Wang X	Int J Syst Evol Microbiol	10.1099/ijsem.0.004990	2021
Phylogeny	Actinoplanes hulinensis sp. nov., a novel actinomycete isolated from soybean root (Glycine max (L.) Merr).	Shen Y, Liu C, Wang X, Zhao J, Jia F, Zhang Y, Wang L, Yang D, Xiang W	Antonie Van Leeuwenhoek	10.1007/s10482-012-9809-9	2012

References

#10818	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43148
#19940	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Actinoplanes campanulatus (DSM 43148, ATCC 15348, CBS 190.64)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_014191935

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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