Name: Nesterenkonia sandarakina DSM 15664
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7767

Type strain:

Species: Nesterenkonia sandarakina

Culture col. no.: DSM 15664, CCTCC AA 203007, CIP 108680, KCTC 19011, YIM 70009, JCM 13020

Strain history: CIP <- 2005, DSMZ <- W.L. Li

NCBI tax ID(s): 272918 (species)

SI-ID 265479

DSM 15664, KCTC 19011, CIP 108680, JCM 13020, CIP 107967

Section

Nesterenkonia sandarakina DSM 15664 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from soil.

coccus-shaped
Gram-positive
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Micrococcaceae*
	Genus	*Nesterenkonia*
	Species	**Nesterenkonia sandarakina**
	Full Scientific Name (LPSN)	Nesterenkonia sandarakina Li et al. 2005

Information on morphological and physiological properties Morphology

[Ref.: #31313]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive
[Ref.: #122412]	Gram stain	negative

[Ref.: #31313]	Cell shape	coccus-shaped
[Ref.: #122412]	Cell shape	oval-shaped

[Ref.: #31313]	Motility	no
[Ref.: #122412]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6019]

Culture medium

ISP2 MEDIUM (DSMZ Medium 987)

[Ref.: #6019]

Culture medium growth

[Ref.: #6019]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6019]

Culture medium composition

expand / minimize

[Ref.: #42043]

Culture medium

Marine agar (MA)

[Ref.: #42043]

Culture medium growth

[Ref.: #42043]

Culture medium composition

expand / minimize

[Ref.: #122412]

Culture medium

CIP Medium 13

[Ref.: #122412]

Culture medium growth

[Ref.: #122412]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #6019]		growth	28 °C
[Ref.: #42043]		growth	30 °C
[Ref.: #67770]		growth	28 °C
[Ref.: #67771]		growth	30 °C
[Ref.: #122412]		growth	10-30 °C
[Ref.: #122412]	no	growth	37 °C
[Ref.: #122412]	no	growth	41 °C

[Ref.: #6019]	Temperature range	mesophilic
[Ref.: #42043]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic
[Ref.: #122412]	Temperature range	mesophilic
[Ref.: #122412]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #31313]			optimum	8.5
[Ref.: #31313]			growth	05-12

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67771]	Oxygen tolerance	aerobe
[Ref.: #122412]	Oxygen tolerance	obligate aerobe

[Ref.: #31313]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31313]		NaCl		growth	01-15	%
[Ref.: #31313]		NaCl		optimum	5	%

[Ref.: #6019]	Murein short key	A11.42
[Ref.: #6019]	Murein types	A4alpha L-Lys-Gly-D-Asp

[Ref.: #67770]	Observation	quinones: MK-7, MK-8
[Ref.: #67771]	Observation	quinones: MK-7, MK-8

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31313]	arabinose ChEBI	+	carbon source
[Ref.: #31313]	cellobiose ChEBI	+	carbon source
[Ref.: #31313]	dextrin ChEBI	+	carbon source
[Ref.: #31313]	fructose ChEBI	+	carbon source
[Ref.: #31313]	galactose ChEBI	+	carbon source
[Ref.: #31313]	glucose ChEBI	+	carbon source
[Ref.: #122412]	hippurate ChEBI	-	hydrolysis
[Ref.: #31313]	maltose ChEBI	+	carbon source
[Ref.: #31313]	mannitol ChEBI	+	carbon source
[Ref.: #31313]	mannose ChEBI	+	carbon source
[Ref.: #122412]	nitrate ChEBI	-	reduction
[Ref.: #122412]	nitrite ChEBI	-	reduction
[Ref.: #31313]	ribose ChEBI	+	carbon source
[Ref.: #31313]	salicin ChEBI	+	carbon source
[Ref.: #31313]	sucrose ChEBI	+	carbon source
[Ref.: #31313]	trehalose ChEBI	+	carbon source
[Ref.: #31313]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #122412]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #122412]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #122412]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #122412]	amylase	-
[Ref.: #122412]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #122412]	caseinase	+	3.4.21.50
[Ref.: #31313]	catalase	+	1.11.1.6
[Ref.: #122412]	catalase	+	1.11.1.6
[Ref.: #122412]	coagulase	-
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #122412]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #122412]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #122412]	gelatinase	+/-
[Ref.: #122412]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #122412]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #122412]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #122412]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #122412]	oxidase	-
[Ref.: #122412]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #122412]	tween esterase	-
[Ref.: #122412]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122412]	11383	-	-	+	+	-	+	+	-	+	-	-	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6019]	Sample type/isolated from	soil
[Ref.: #6019]	Geographic location (country and/or sea, region)	eastern desert
[Ref.: #6019]	Country	Egypt
[Ref.: #6019]	Country ISO 3 Code	EGY
[Ref.: #6019]	Continent	Africa

[Ref.: #67770]	Sample type/isolated from	Desert soil
[Ref.: #67770]	Country	Egypt
[Ref.: #67770]	Country ISO 3 Code	EGY
[Ref.: #67770]	Continent	Africa

[Ref.: #67771]	Sample type/isolated from	Desert soil
[Ref.: #67771]	Country	Egypt
[Ref.: #67771]	Country ISO 3 Code	EGY
[Ref.: #67771]	Continent	Africa

[Ref.: #122412]	Sample type/isolated from	Environment, Soil sample
[Ref.: #122412]	Geographic location (country and/or sea, region)	Eastern desert
[Ref.: #122412]	Country	Egypt
[Ref.: #122412]	Country ISO 3 Code	EGY
[Ref.: #122412]	Continent	Africa
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY588277 (>99% sequence identity) for Nesterenkonia from Microbeatlas

Aquatic Samples	600
Soil Samples	791
Animal Samples	2130
Plant Samples	449
Total Samples	3970

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6019]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122412]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6019]	Nesterenkonia sandarakina strain YIM 70009 16S ribosomal RNA gene, partial sequence	AY588277	1461	ENA	272918 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Nesterenkonia sandarakina DSM 15664	GCA_013410215	contig	GenBank	272918 tax ID
[Ref.: #66792]	Nesterenkonia sandarakina strain DSM 15664	272918.6	wgs	PATRIC	272918 tax ID	*
[Ref.: #66792]	Nesterenkonia sandarakina DSM 15664	2856487714	draft	JGI IMG/M	272918 tax ID	*

[Ref.: #6019]	GC-content	64 mol%
[Ref.: #67770]	GC-content	64 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67771]	GC-content	64.0 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #6019]

Culture collection no.

DSM 15664, CCTCC AA 203007, CIP 108680, KCTC 19011, YIM 70009, JCM 13020

[Ref.: #77210]

SI-ID 265479

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nesterenkonia aurantiaca sp. nov., an alkaliphilic actinobacterium isolated from Antarctica.	Finore I, Orlando P, Di Donato P, Leone L, Nicolaus B, Poli A	Int J Syst Evol Microbiol	10.1099/ijsem.0.000917	2016	*
Phylogeny	Nesterenkonia sandarakina sp. nov. and Nesterenkonia lutea sp. nov., novel actinobacteria, and emended description of the genus Nesterenkonia.	Li WJ, Chen HH, Kim CJ, Zhang YQ, Park DJ, Lee JC, Xu LH, Jiang CL	Int J Syst Evol Microbiol	10.1099/ijs.0.63281-0	2005	*

References

#6019

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15664

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31313

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27628 (see below)

#42043 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#77210

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID265479.1 )

#122412 Collection of Institut Pasteur ; Curators of the CIP; CIP 108680

#27628

IJSEM 463 2005 ( DOI 10.1099/ijs.0.63281-0 , PubMed 15653919 )

* These data were automatically processed and therefore are not curated

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