Name: Nesterenkonia halotolerans DSM 15474
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 7764

Type strain:

Species: Nesterenkonia halotolerans

Culture col. no.: DSM 15474, CCTCC AA 001022, YIM 70084, JCM 13018, KCTC 19014

Strain history: KCTC 19014 <-- W.-J. Li YIM 70084.

NCBI tax ID(s): 225325 (species)

SI-ID 132022

DSM 15474, JCM 13018, KCTC 19014

Special Collections

Section

Nesterenkonia halotolerans DSM 15474 is a Gram-positive, motile, coccus-shaped bacterium that was isolated from soil.

coccus-shaped
Gram-positive
motile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Micrococcaceae*
	Genus	*Nesterenkonia*
	Species	**Nesterenkonia halotolerans**
	Full Scientific Name (LPSN)	Nesterenkonia halotolerans Li et al. 2004

Information on morphological and physiological properties Morphology

[Ref.: #30096]	Gram stain	positive

[Ref.: #30096]	Cell shape	coccus-shaped

[Ref.: #30096]	Motility	yes

[Ref.: #30096]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5944]

Culture medium

NESTERENKONIA MODIFIED MEDIUM (DSMZ Medium 993a)

[Ref.: #5944]

Culture medium growth

[Ref.: #5944]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5944]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #5944]	growth	28 °C
[Ref.: #30096]	growth	04-40 °C
[Ref.: #30096]	optimum	28 °C
[Ref.: #67770]	growth	28 °C

	pH	Kind of pH		pH
[Ref.: #30096]			growth	07-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30096]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30096]		NaCl		growth	0-25	%
[Ref.: #30096]		NaCl		optimum	10	%

[Ref.: #5944]	Murein short key	A11.42
[Ref.: #5944]	Murein types	A4alpha L-Lys-Gly-D-Asp

[Ref.: #67770]

Observation

quinones: MK-7, MK-8

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30096]	dextrin ChEBI	+	carbon source
[Ref.: #30096]	fructose ChEBI	+	carbon source
[Ref.: #30096]	galactose ChEBI	+	carbon source
[Ref.: #30096]	gelatin ChEBI	+	carbon source
[Ref.: #30096]	glucose ChEBI	+	carbon source
[Ref.: #30096]	lactose ChEBI	+	carbon source
[Ref.: #30096]	maltose ChEBI	+	carbon source
[Ref.: #30096]	mannose ChEBI	+	carbon source
[Ref.: #30096]	sucrose ChEBI	+	carbon source
[Ref.: #30096]	urea ChEBI	+	carbon source

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #30096]		gelatinase	+
[Ref.: #30096]		urease	+	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5944]	Sample type/isolated from	soil
[Ref.: #5944]	Geographic location (country and/or sea, region)	Xijiang
[Ref.: #5944]	Country	China
[Ref.: #5944]	Country ISO 3 Code	CHN
[Ref.: #5944]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Hypersaline soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	Xinjang Province
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY226508 (>99% sequence identity) for Nesterenkonia from Microbeatlas

Aquatic Samples	600
Soil Samples	791
Animal Samples	2130
Plant Samples	449
Total Samples	3970

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5944]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5944]	Nesterenkonia sp. YIM 70084 16S ribosomal RNA gene, partial sequence	AY226508	1490	GenBank ENA	225325 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Nesterenkonia halotolerans DSM 15474	GCA_014874065	contig	GenBank ENA	225325 tax ID	*
[Ref.: #66792]	Nesterenkonia halotolerans strain DSM 15474	225325.3	wgs	PATRIC	225325 tax ID	*
[Ref.: #66792]	Nesterenkonia halotolerans DSM 15474	2870876733	draft	JGI IMG/M	225325 tax ID	*

[Ref.: #30096]	GC-content	64.4 mol%
[Ref.: #67770]	GC-content	64.4 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Nesterenkonia halotolerans strain DSM 15474 PATRIC: 225325.3
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Nesterenkonia halotolerans strain DSM 15474 PATRIC: 225325.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	88.342	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	97.515	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	77.774	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	91.514	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	89.166	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	75.5	no

Availability in culture collections External links

[Ref.: #5944]

Culture collection no.

DSM 15474, CCTCC AA 001022, YIM 70084, JCM 13018, KCTC 19014

[Ref.: #77207]

SI-ID 132022

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nesterenkonia aurantiaca sp. nov., an alkaliphilic actinobacterium isolated from Antarctica.	Finore I, Orlando P, Di Donato P, Leone L, Nicolaus B, Poli A	Int J Syst Evol Microbiol	10.1099/ijsem.0.000917	2016	*
Phylogeny	Nesterenkonia halotolerans sp. nov. and Nesterenkonia xinjiangensis sp. nov., actinobacteria from saline soils in the west of China.	Li WJ, Chen HH, Zhang YQ, Schumann P, Stackebrandt E, Xu LH, Jiang CL	Int J Syst Evol Microbiol	10.1099/ijs.0.02935-0	2004	*

References

#5944

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15474

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30096

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26452 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#77207

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID132022.1 )

#26452

IJSEM 837 2004 ( DOI 10.1099/ijs.0.02935-0 , PubMed 15143032 )

* These data were automatically processed and therefore are not curated

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Nesterenkonia halotolerans strain DSM 15474

Nesterenkonia halotolerans strain DSM 15474

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