Kocuria rhizophila TA68 is an aerobe, Gram-positive, coccus-shaped bacterium that was isolated from rhizosphere of Typha angustiflora.
Gram-positive coccus-shaped aerobe genome sequence 16S sequence Bacteria| @ref 20215 |
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| Domain Bacteria |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Micrococcales |
| Family Micrococcaceae |
| Genus Kocuria |
| Species Kocuria rhizophila |
| Full scientific name Kocuria rhizophila Kovács et al. 1999 |
| @ref | Colony color | Incubation period | Medium used | Hemolysis ability | |
|---|---|---|---|---|---|
| 18361 | Sulfur yellow (1016) | 10-14 days | ISP 2 | ||
| 18361 | Sulfur yellow (1016) | 10-14 days | ISP 3 | ||
| 18361 | Sulfur yellow (1016) | 10-14 days | ISP 4 | ||
| 18361 | Sulfur yellow (1016) | 10-14 days | ISP 5 | ||
| 18361 | Sulfur yellow (1016) | 10-14 days | ISP 6 | ||
| 18361 | Sulfur yellow (1016) | 10-14 days | ISP 7 | ||
| 122201 | 0 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 4541 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | Medium recipe at MediaDive | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water | ||
| 18361 | ISP 2 | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |||
| 18361 | ISP 7 | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 18361 | ISP 6 | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 18361 | ISP 5 | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |||
| 18361 | ISP 4 | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |||
| 18361 | ISP 3 | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |||
| 41794 | MEDIUM 307 - for Kocuria rhizophila | Distilled water make up to (1000.000 ml);Yeast extract (3.000 g);Tryptocasein soy agar (40.000 g) | |||
| 122201 | CIP Medium 72 | Medium recipe at CIP | |||
| 122201 | CIP Medium 307 | Medium recipe at CIP |
| @ref | Salt | Growth | Tested relation | Concentration | |
|---|---|---|---|---|---|
| 122201 | NaCl | positive | growth | 6.5 % |
| @ref | Murein short key | Type | |
|---|---|---|---|
| 4541 | A11.06 | A3alpha L-Lys-L-Ala3 |
| 67770 | Observationquinones: MK-7(H2), MK-8(H2) |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 68379 | 17634 ChEBI | D-glucose | + | fermentation | from API Coryne |
| 68379 | 16899 ChEBI | D-mannitol | - | fermentation | from API Coryne |
| 68379 | 16988 ChEBI | D-ribose | - | fermentation | from API Coryne |
| 68379 | 65327 ChEBI | D-xylose | - | fermentation | from API Coryne |
| 68379 | 4853 ChEBI | esculin | - | hydrolysis | from API Coryne |
| 68379 | 5291 ChEBI | gelatin | - | hydrolysis | from API Coryne |
| 68379 | 28087 ChEBI | glycogen | - | fermentation | from API Coryne |
| 122201 | 606565 ChEBI | hippurate | - | hydrolysis | |
| 68379 | 17716 ChEBI | lactose | - | fermentation | from API Coryne |
| 68379 | 17306 ChEBI | maltose | + | fermentation | from API Coryne |
| 68379 | 17632 ChEBI | nitrate | - | reduction | from API Coryne |
| 122201 | 17632 ChEBI | nitrate | - | reduction | |
| 122201 | 16301 ChEBI | nitrite | - | reduction | |
| 68379 | 17992 ChEBI | sucrose | - | fermentation | from API Coryne |
| 68379 | 16199 ChEBI | urea | + | hydrolysis | from API Coryne |
| @ref | Metabolite | Is sensitive | Is resistant | |
|---|---|---|---|---|
| 122201 | 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate) |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 122201 | alcohol dehydrogenase | - | 1.1.1.1 | |
| 68379 | alkaline phosphatase | - | 3.1.3.1 | from API Coryne |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | - | 3.2.1.22 | from API zym |
| 68382 | alpha-glucosidase | + | 3.2.1.20 | from API zym |
| 68379 | alpha-glucosidase | + | 3.2.1.20 | from API Coryne |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 122201 | amylase | - | ||
| 68382 | beta-galactosidase | - | 3.2.1.23 | from API zym |
| 122201 | beta-galactosidase | - | 3.2.1.23 | |
| 68379 | beta-galactosidase | - | 3.2.1.23 | from API Coryne |
| 68382 | beta-glucosidase | - | 3.2.1.21 | from API zym |
| 68379 | beta-glucosidase | - | 3.2.1.21 | from API Coryne |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 68379 | beta-glucuronidase | - | 3.2.1.31 | from API Coryne |
| 122201 | caseinase | + | 3.4.21.50 | |
| 122201 | catalase | + | 1.11.1.6 | |
| 122201 | coagulase | - | ||
| 122201 | DNase | - | ||
| 68382 | esterase (C 4) | + | from API zym | |
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 122201 | gamma-glutamyltransferase | + | 2.3.2.2 | |
| 122201 | gelatinase | +/- | ||
| 68379 | gelatinase | - | from API Coryne | |
| 122201 | lecithinase | - | ||
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 122201 | lipase | - | ||
| 68382 | lipase (C 14) | + | from API zym | |
| 122201 | lysine decarboxylase | - | 4.1.1.18 | |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API Coryne |
| 122201 | ornithine decarboxylase | - | 4.1.1.17 | |
| 122201 | oxidase | - | ||
| 122201 | protease | - | ||
| 68379 | pyrazinamidase | - | 3.5.1.B15 | from API Coryne |
| 68379 | pyrrolidonyl arylamidase | + | 3.4.19.3 | from API Coryne |
| 122201 | tween esterase | - | ||
| 122201 | urease | - | 3.5.1.5 | |
| 68379 | urease | + | 3.5.1.5 | from API Coryne |
| Cat1 | Cat2 | Cat3 | |
|---|---|---|---|
| #Host | #Plants | #Herbaceous plants (Grass,Crops) | |
| #Host Body-Site | #Plant | #Rhizosphere |
| @ref | Sample type | Host species | Geographic location | Country | Country ISO 3 Code | Continent | Isolation date | |
|---|---|---|---|---|---|---|---|---|
| 4541 | rhizosphere of Typha angustiflora | Typha angustifolia | Soroksar river (tributary of Danube river) | Hungary | HUN | Europe | ||
| 60716 | Rhizosphere of narrow-leaved cattail | Danube river | Hungary | HUN | Europe | |||
| 67770 | Rhizosphere of narrow-leaved cattail (Typha angustifolia) from a floating mat in the Soroksár tributary of the River Danube | Typha angustifolia | Hungary | HUN | Europe | |||
| 122201 | Environment, Rhizosphere of Typha angustiflora | Soroksar river | Hungary | HUN | Europe | 1995 |
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 67770 | ASM366722v1 assembly for Kocuria rhizophila TA68 | contig | 72000 | 40.24 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 67770 | Kocuria rhizophila 16S rRNA gene | Y16264 | 1471 | 72000 | ||
| 124043 | Kocuria rhizophila strain DSM 11926(T) 16S ribosomal RNA gene, partial sequence. | MN688241 | 525 | 72000 | ||
| 124043 | Kocuria rhizophila strain DSM 11926(T) 16S ribosomal RNA gene, partial sequence. | MN686665 | 533 | 72000 |
| @ref | GC-content (mol%) | Method | |
|---|---|---|---|
| 4541 | 69.4 | high performance liquid chromatography (HPLC) |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positiveⓘ | yes | 94.13 | no |
| 125438 | anaerobic | anaerobicⓘ | no | 96.95 | yes |
| 125438 | aerobic | aerobicⓘ | yes | 84.76 | no |
| 125438 | spore-forming | spore-formingⓘ | no | 77.28 | no |
| 125438 | thermophilic | thermophileⓘ | no | 97.00 | yes |
| 125438 | flagellated | motile2+ⓘ | no | 94.00 | no |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Genetics | The Kocurious case of Noodlococcus: genomic insights into Kocuria rhizophila from characterisation of a laboratory contaminant. | McCallum GE, Ho SFS, Cummins EA, Wildsmith AJ, McInnes RS, Weigel C, Tong LYS, Quick J, van Schaik W, Moran RA. | Microb Genom | 10.1099/mgen.0.001526 | 2025 | |
| Great Tit (Parus major) Uropygial Gland Microbiomes and Their Potential Defensive Roles. | Bodawatta KH, Schierbech SK, Petersen NR, Sam K, Bos N, Jonsson KA, Poulsen M. | Front Microbiol | 10.3389/fmicb.2020.01735 | 2020 | ||
| Metabolism | An Endophytic Bacterial Consortium modulates multiple strategies to improve Arsenic Phytoremediation Efficacy in Solanum nigrum. | Mukherjee G, Saha C, Naskar N, Mukherjee A, Mukherjee A, Lahiri S, Majumder AL, Seal A. | Sci Rep | 10.1038/s41598-018-25306-x | 2018 | |
| Persistent bloodstream infection with Kocuria rhizophila related to a damaged central catheter. | Moissenet D, Becker K, Merens A, Ferroni A, Dubern B, Vu-Thien H. | J Clin Microbiol | 10.1128/jcm.06038-11 | 2012 | ||
| Metabolism | Antimicrobial peptide expression in a wild tobacco plant reveals the limits of host-microbe-manipulations in the field. | Weinhold A, Karimi Dorcheh E, Li R, Rameshkumar N, Baldwin IT. | Elife | 10.7554/elife.28715 | 2018 | |
| Enzymology | Two cases of peritonitis caused by Kocuria marina in patients undergoing continuous ambulatory peritoneal dialysis. | Lee JY, Kim SH, Jeong HS, Oh SH, Kim HR, Kim YH, Lee JN, Kook JK, Kho WG, Bae IK, Shin JH. | J Clin Microbiol | 10.1128/jcm.00847-09 | 2009 | |
| Development of a Novel Escherichia coli-Kocuria Shuttle Vector Using the Cryptic pKPAL3 Plasmid from K. palustris IPUFS-1 and Its Utilization in Producing Enantiopure (S)-Styrene Oxide. | Toda H, Itoh N. | Front Microbiol | 10.3389/fmicb.2017.02313 | 2017 | ||
| Pathogenicity | Antibiotic-resistant bacteria in the guts of insects feeding on plants: prospects for discovering plant-derived antibiotics. | Ignasiak K, Maxwell A. | BMC Microbiol | 10.1186/s12866-017-1133-0 | 2017 | |
| Phylogeny | Kocuria tytonicola, new bacteria from the preen glands of American barn owls (Tyto furcata). | Braun MS, Wang E, Zimmermann S, Boutin S, Wagner H, Wink M | Syst Appl Microbiol | 10.1016/j.syapm.2018.11.004 | 2018 | |
| Phylogeny | Kocuria tytonis sp. nov., isolated from the uropygial gland of an American barn owl (Tyto furcata). | Braun MS, Wang E, Zimmermann S, Wagner H, Wink M | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003170 | 2019 | |
| Phylogeny | Kocuria polaris sp. nov., an orange-pigmented psychrophilic bacterium isolated from an Antarctic cyanobacterial mat sample. | Reddy GSN, Prakash JSS, Prabahar V, Matsumoto GI, Stackebrandt E, Shivaji S | Int J Syst Evol Microbiol | 10.1099/ijs.0.02336-0 | 2003 | |
| Phylogeny | Kocuria palustris sp. nov. and Kocuria rhizophila sp. nov., isolated from the rhizoplane of the narrow-leaved cattail (Typha angustifolia). | Kovacs G, Burghardt J, Pradella S, Schumann P, Stackebrandt E, Marialigeti K | Int J Syst Bacteriol | 10.1099/00207713-49-1-167 | 1999 |
| #4541 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 11926 |
| #18361 | Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig . |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #41794 | ; Curators of the CIP; |
| #60716 | Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 55752 |
| #67770 | Japan Collection of Microorganism (JCM) ; Curators of the JCM; |
| #68379 | Automatically annotated from API Coryne . |
| #68382 | Automatically annotated from API zym . |
| #122201 | Collection of Institut Pasteur ; Curators of the CIP; CIP 105972 |
| #124043 | Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy . |
| #125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 ) |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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