Name: Kocuria varians G 33
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7642

Type strain:

Species: Kocuria varians

Strain Designation: G 33

Culture col. no.: DSM 20033, ATCC 15306, CCM 884, NCDO 777, NCTC 7564, JCM 7238, BCRC 12152, CCUG 35392, CCUG 3705, CECT 230, CIP 81.73, GIFU 9844, HAMBI 1951, HAMBI 40, IAM 13594, IFO 15358, LMG 14231, NBRC 15358, NCFB 624, NCFB 777, NCIMB 11697, VKM Ac-2215, VKM B-1232, VKM B-1827, VTT E-97857

Strain history: GIFU 9844 <-- ATCC 15306 <-- T. Gibson G33 ("Staphylococcus lactis").

NCBI tax ID(s): 1272 (species)

Other strains from Kocuria varians

K. varians STI09587(IMET), A-6, IMET 9587
K. varians CCUG 30959
K. varians CCUG 37929
K. varians CCUG 51540
K. varians JCM 20145, ATCC 399, CCM 2492, ...
K. varians JCM 20436, ATCC 383, CCM 2490, ...

Section

Kocuria varians G 33 is a mesophilic bacterium that was isolated from milk.

mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Micrococcaceae*
	Genus	*Kocuria*
	Species	**Kocuria varians**
	Full Scientific Name (PNU)	Kocuria varians (Migula 1900) Stackebrandt et al. 1995

Synonym

Micrococcus varians

Information on morphological and physiological properties Morphology

[Ref.: #18619]	Colony color	Lemon yellow (1012)
[Ref.: #18619]	Incubation period	10-14 days
[Ref.: #18619]	Cultivation medium used	5006

[Ref.: #18619]	Colony color	Lemon yellow (1012)
[Ref.: #18619]	Incubation period	10-14 days
[Ref.: #18619]	Cultivation medium used	5425

[Ref.: #18619]	Colony color	Lemon yellow (1012)
[Ref.: #18619]	Incubation period	10-14 days
[Ref.: #18619]	Cultivation medium used	5428

[Ref.: #18619]	Colony color	Lemon yellow (1012)
[Ref.: #18619]	Incubation period	10-14 days
[Ref.: #18619]	Cultivation medium used	5530

[Ref.: #18619]	Colony color	Lemon yellow (1012)
[Ref.: #18619]	Incubation period	10-14 days
[Ref.: #18619]	Cultivation medium used	ISP 2

[Ref.: #18619]	Multicellular complex forming ability	no
[Ref.: #18619]	Medium Name (multicellularity)	ISP 2

[Ref.: #18619]	Multicellular complex forming ability	no
[Ref.: #18619]	Medium Name (multicellularity)	5006

[Ref.: #18619]	Multicellular complex forming ability	no
[Ref.: #18619]	Medium Name (multicellularity)	5425

[Ref.: #18619]	Multicellular complex forming ability	no
[Ref.: #18619]	Medium Name (multicellularity)	5428

[Ref.: #18619]	Multicellular complex forming ability	no
[Ref.: #18619]	Medium Name (multicellularity)	5530

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8441]

Culture medium

CORYNEBACTERIUM AGAR (DSMZ Medium 53)

[Ref.: #8441]

Culture medium growth

[Ref.: #8441]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #8441]

Culture medium composition

expand / minimize

[Ref.: #18619]

Culture medium

ISP 2

[Ref.: #18619]

Culture medium growth

[Ref.: #18619]

Culture medium composition

expand / minimize

[Ref.: #18619]

Culture medium

5006

[Ref.: #18619]

Culture medium growth

[Ref.: #18619]

Culture medium composition

expand / minimize

[Ref.: #18619]

Culture medium

5425

[Ref.: #18619]

Culture medium growth

[Ref.: #18619]

Culture medium composition

expand / minimize

[Ref.: #18619]

Culture medium

5428

[Ref.: #18619]

Culture medium growth

[Ref.: #18619]

Culture medium

5530

[Ref.: #18619]

Culture medium growth

[Ref.: #37627]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #37627]

Culture medium growth

[Ref.: #37627]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #8441]	growth	30 °C
[Ref.: #18619]	optimum	28 °C
[Ref.: #37627]	growth	30 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #8441]	Temperature range	mesophilic
[Ref.: #18619]	Temperature range	mesophilic
[Ref.: #37627]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #8441]	Murein short key	A11.07
[Ref.: #8441]	Murein types	A3alpha L-Lys-L-Ala₄

[Ref.: #67770]

Observation

quinones: MK-7(H_n), MK-7(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68375]	arabinose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	arginine ChEBI	-	hydrolysis	* from API ID32STA
[Ref.: #68375]	cellobiose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	D-fructose ChEBI	+	fermentation	* from API ID32STA
[Ref.: #68375]	D-glucose ChEBI	+	fermentation	* from API ID32STA, API Coryne
[Ref.: #68375]	D-mannitol ChEBI	-	fermentation	* from API ID32STA, API Coryne
[Ref.: #68375]	D-mannose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	D-ribose ChEBI	-	fermentation	* from API ID32STA, API Coryne
[Ref.: #68379]	D-xylose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #68375]	esculin ChEBI	-	hydrolysis	* from API ID32STA
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #68379]	glycogen ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68375]	lactose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68379]	maltose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68375]	maltose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	N-acetylglucosamine ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	nitrate ChEBI	+	reduction	* from API ID32STA
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #68375]	ornithine ChEBI	-	degradation	* from API ID32STA
[Ref.: #68375]	raffinose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68379]	sucrose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68375]	sucrose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	trehalose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	turanose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68379]	urea ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #68375]	urea ChEBI	-	hydrolysis	* from API ID32STA
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #68375]		novobiocin ChEBI	yes	1.8	µg	* from API ID32STA

	Metabolite production	Metabolite
[Ref.: #68375]		acetoin ChEBI	* from API ID32STA

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #68375]		acetoin ChEBI	-	* from API ID32STA

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym, API ID32STA, API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68375]	arginine dihydrolase	-	3.5.3.6	* from API ID32STA
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym, API ID32STA, API Coryne
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym, API Coryne
[Ref.: #68375]	beta-glucosidase	-	3.2.1.21	* from API ID32STA
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API ID32STA, API Coryne
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68375]	L-arginine arylamidase	-		* from API ID32STA
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym, API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68375]	ornithine decarboxylase	-	4.1.1.17	* from API ID32STA
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #68375]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API ID32STA
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	+	3.5.1.5	* from API Coryne
[Ref.: #68375]	urease	-	3.5.1.5	* from API ID32STA
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #18619]	171	-	-	+	-	-	+	+	+	+	+	-	not determinedn.d.	+	-	+	-	+	+	+	+	not determinedn.d.

API® ID32STA:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	Ornithine decarboxylaseODC	Esculin hydrolysisESC	Fermentation of D-glucoseGLU	Fermentation of D-fructoseFRU	Fermentation of D-mannoseMNE	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of trehaloseTRE	Fermentation of D-mannitolMAN	Fermentation of raffinoseRAF	Fermentation of D-riboseRIB	Fermentation of cellobioseCEL	Reduction of nitrateNIT	Acetoin production (Voges Proskauer test)VP	beta-Galactosidasebeta GAL	L-arginine arylamidaseArgA	Alkaline phosphatasePAL	Pyrrolidonyl arylamidasePyrA	Novobiocin resistanceNOVO	Fermentation of sucroseSAC	Fermentation of N-acetyl-glucosamineNAG	Fermentation of turanoseTUR	Fermentation of arabinoseARA	beta-Glucuronidasebeta GUR
[Ref.: #8441]	5530	-	-	-	-	+	+	-	-	-	-	-	-	-	-	+	-	+	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18619]	258	not determinedn.d.	-	-	+	-	+	-	-	-	-	+	-	+	+	-	+	+	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8441]	Sample type/isolated from	milk

[Ref.: #67770]	Sample type/isolated from	Milk
* marker position based on {}

Isolation sources categories

#Host Body Product	#Fluids	#Milk
#Engineered	#Food production	#Dairy product

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence X87754 (>99% sequence identity) for Kocuria varians from Microbeatlas

Aquatic Samples	81
Soil Samples	44
Animal Samples	516
Plant Samples	10
Total Samples	651

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18619]	Biosafety level	1	Hazard group
[Ref.: #8441]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8441]	M.varians 16S rRNA gene	X87754	1481	ENA	1272 tax ID
[Ref.: #20218]	Kocuria varians 16S ribosomal RNA gene, partial sequence	AF542074	1333	ENA	1272 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Kocuria varians NBRC 15358	GCA_006539885	contig	GenBank	1272 tax ID
[Ref.: #66792]	Kocuria varians strain NBRC 15358	1272.7	wgs	PATRIC	1272 tax ID	*

[Ref.: #8441]	GC-content	69.0 mol%
[Ref.: #67770]	GC-content	69 mol%

Availability in culture collections External links

[Ref.: #8441]

Culture collection no.

DSM 20033, ATCC 15306, CCM 884, NCDO 777, NCTC 7564, JCM 7238, BCRC 12152, CCUG 35392, CCUG 3705, CECT 230, CIP 81.73, GIFU 9844, HAMBI 1951, HAMBI 40, IAM 13594, IFO 15358, LMG 14231, NBRC 15358, NCFB 624, NCFB 777, NCIMB 11697, VKM Ac-2215, VKM B-1232, VKM B-1827, VTT E-97857

[Ref.: #20218]

Associated Passport(s) in StrainInfo

54882, 54878, 54879, 163976, 54890

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Kocuria tytonis sp. nov., isolated from the uropygial gland of an American barn owl (Tyto furcata).	Braun MS, Wang E, Zimmermann S, Wagner H, Wink M	Int J Syst Evol Microbiol	10.1099/ijsem.0.003170	2019	*
Phylogeny	Kocuria polaris sp. nov., an orange-pigmented psychrophilic bacterium isolated from an Antarctic cyanobacterial mat sample.	Reddy GSN, Prakash JSS, Prabahar V, Matsumoto GI, Stackebrandt E, Shivaji S	Int J Syst Evol Microbiol	10.1099/ijs.0.02336-0	2003	*

References

#8441

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20033

#18619

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#37627 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68375

Automatically annotated from API ID32STA .

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Kocuria varians in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF