Rothia kristinae (DSM 20032, ATCC 27570, CCM 2690)

Name: Rothia kristinae (DSM 20032, ATCC 27570, CCM 2690)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7637

Type strain

Strain Designation PM 129

Culture col. no. DSM 20032 ATCC 27570 CCM 2690 CCUG 33026 PM 129 12 more

NCBI tax ID(s) 1349768 37923

Special Collections DSMZ CCUG JCM CIP Wink compendium

Links

Rothia kristinae PM 129 is a facultative anaerobe, mesophilic, Gram-positive prokaryote that was isolated from human skin.

Gram-positive coccus-shaped facultative anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 14405

LMG 14215,ATCC 27570,CCM 2690,DSM 20032,IAM 13590,IFO 15354,NCTC 11038,CCUG 33026,JCM 7237,VKM B-1811,CIP 81.69,IMET 11367,NBRC 15354,NRRL B-14835,CIP 81,VKM Ac-2190,VTT E-97856

@ref 20215

Last LPSN update 2025-12-16 09:48:38

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Micrococcaceae

Genus Rothia

Species Rothia kristinae

Full scientific name Rothia kristinae (Kloos et al. 1974) Nouioui et al. 2018

Synonyms (2)

Micrococcus kristinae
Kocuria kristinae

BacDive ID	Other strains from **Rothia kristinae** (16)	Type strain
7636	R. kristinae DSM 20027, Back 228c
7638	R. kristinae AW 204, DSM 20321, ATCC 27572, CCM 2692, ...
103063	R. kristinae STI09591(IMET), N3704, IMET 9591
135300	R. kristinae KH 189, CIP 82.53, ATCC 27571, CCM 2691
146264	R. kristinae CCUG 30744 B
147237	R. kristinae CCUG 33141
150701	R. kristinae CCUG 41986 B
151722	R. kristinae CCUG 44709
152031	R. kristinae CCUG 45603
152102	R. kristinae CCUG 45788
152714	R. kristinae CCUG 47458
154149	R. kristinae CCUG 52569
154278	R. kristinae CCUG 52954
154876	R. kristinae CCUG 55514
154961	R. kristinae CCUG 55878, ATCC BAA-752
158996	R. kristinae Fh040319-C18, DSM 109732

Morphology

Cell morphology

@ref	Gram stain	Cell shape
120502	positive	coccus-shaped
125438
125438	positive
125439	positive

Colony morphology

@ref	Colony color	Incubation period	Medium used
18416	Cream (9001)	10-14 days	5006
18416	Cream (9001)	10-14 days	5425
18416	Cream (9001)	10-14 days	5428
18416	Cream (9001)	10-14 days	5530
18416	Cream (9001)	10-14 days	ISP 2
120502

Multicellular morphology

@ref	Forms multicellular complex	Medium name
18416		ISP 2
18416		5006
18416		5425
18416		5428
18416		5530

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8440	CORYNEBACTERIUM AGAR (DSMZ Medium 53)	Medium recipe at MediaDive	Name: CORYNEBACTERIUM AGAR (DSMZ Medium 53) Composition: Agar 15.0 g/l Casein peptone 10.0 g/l NaCl 5.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled water
18416	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18416	5006		Medium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes
18416	5425		Name: Brain Heart Infusion (BHI) Agar; 5425 Composition: Calf brain infusion 200.0 g/l Beef heart infusion 250.0 g/l Casein (meat) peptone 10.0 g/l Glucose 2.0 g/l NaCl 5.0 g/l Na2HPO4 2.5 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.4 Usage: Maintenance and revitalization Organisms: Dermatophilus congolensis, Nocardia brasieliensis, N. asteroides, Rhodococcus equi, R. fascians, R. rhodochrous, Streptomyces thermoviol aceus subsp. apingens, Coryneform species
18416	5428
18416	5530
40056	MEDIUM 29- Brain heart agar		Distilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
120502	CIP Medium 29	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
8440	positive	growth	37	mesophilic
18416	positive	optimum	28	mesophilic
40056	positive	growth	30	mesophilic
67770	positive	growth	28	mesophilic
120502	positive	growth	22-41
120502	negative	growth	10
120502	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
120502	facultative anaerobe
125439	obligate aerobe

Compound production

20216

CompoundAldolase

Murein

@ref	Murein short key	Type
8440	A11.07	A3alpha L-Lys-L-Ala₄

Observation

67770

Observationquinones: MK-7(H₂)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	+	builds acid from	from API 50CH acid
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68368	16947 ChEBI	citrate	-	assimilation	from API 20E
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68379	17634 ChEBI	D-glucose	+	fermentation	from API Coryne
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68371	17924 ChEBI	D-sorbitol	+	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68379	4853 ChEBI	esculin	-	hydrolysis	from API Coryne
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68379	5291 ChEBI	gelatin	-	hydrolysis	from API Coryne
68368	5291 ChEBI	gelatin	-	hydrolysis	from API 20E
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	+	builds acid from	from API 50CH acid
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
120502	606565 ChEBI	hippurate	-	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68368	25094 ChEBI	lysine	-	degradation	from API 20E
68379	17306 ChEBI	maltose	+	fermentation	from API Coryne
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	+	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
68379	17632 ChEBI	nitrate	+	reduction	from API Coryne
120502	17632 ChEBI	nitrate	-	reduction
120502	16301 ChEBI	nitrite	-	reduction
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68379	17992 ChEBI	sucrose	-	fermentation	from API Coryne
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68371	32528 ChEBI	turanose	+	builds acid from	from API 50CH acid
68379	16199 ChEBI	urea	-	hydrolysis	from API Coryne
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
120502	35581 ChEBI	indole
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
120502	15688 ChEBI	acetoin	+
68368	15688 ChEBI	acetoin	+	from API 20E
68368	35581 ChEBI	indole		from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
120502	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68379	alkaline phosphatase	-	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
120502	amylase	-
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68382	beta-galactosidase	-	3.2.1.23	from API zym
120502	beta-galactosidase	-	3.2.1.23
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68379	beta-glucosidase	-	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
120502	caseinase	-	3.4.21.50
120502	catalase	+	1.11.1.6
120502	coagulase	-
68382	cystine arylamidase	-	3.4.11.3	from API zym
120502	DNase	-
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
120502	gamma-glutamyltransferase	-	2.3.2.2
120502	gelatinase	+/-
68379	gelatinase	-		from API Coryne
68368	gelatinase	-		from API 20E
120502	lecithinase	-
120502	lipase	-
68382	lipase (C 14)	-		from API zym
120502	lysine decarboxylase	-	4.1.1.18
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
120502	ornithine decarboxylase	-	4.1.1.17
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
120502	oxidase	-
120502	phenylalanine ammonia-lyase	+	4.3.1.24
120502	protease	-
68379	pyrazinamidase	-	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	+	3.4.19.3	from API Coryne
68382	trypsin	-	3.4.21.4	from API zym
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
120502	tween esterase	-
120502	urease	-	3.5.1.5
68379	urease	-	3.5.1.5	from API Coryne
68368	urease	-	3.5.1.5	from API 20E
68382	valine arylamidase	-		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	palmitate biosynthesis	100	22 of 22
66794	lipoate biosynthesis	100	5 of 5
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	vitamin K metabolism	100	5 of 5
66794	formaldehyde oxidation	100	3 of 3
66794	methylglyoxal degradation	100	5 of 5
66794	coenzyme A metabolism	100	4 of 4
66794	acetoin degradation	100	3 of 3
66794	teichoic acid biosynthesis	100	1 of 1
66794	denitrification	100	2 of 2
66794	adipate degradation	100	2 of 2
66794	acetate fermentation	100	4 of 4
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	folate polyglutamylation	100	1 of 1
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	ppGpp biosynthesis	100	4 of 4
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	enterobactin biosynthesis	100	3 of 3
66794	threonine metabolism	90	9 of 10
66794	chorismate metabolism	88.89	8 of 9
66794	heme metabolism	85.71	12 of 14
66794	pentose phosphate pathway	81.82	9 of 11
66794	cellulose degradation	80	4 of 5
66794	glycine betaine biosynthesis	80	4 of 5
66794	photosynthesis	78.57	11 of 14
66794	citric acid cycle	78.57	11 of 14
66794	serine metabolism	77.78	7 of 9
66794	valine metabolism	77.78	7 of 9
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	vitamin B1 metabolism	76.92	10 of 13
66794	phenylalanine metabolism	76.92	10 of 13
66794	sulfopterin metabolism	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	biotin biosynthesis	75	3 of 4
66794	butanoate fermentation	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	isoleucine metabolism	75	6 of 8
66794	flavin biosynthesis	73.33	11 of 15
66794	peptidoglycan biosynthesis	73.33	11 of 15
66794	proline metabolism	72.73	8 of 11
66794	propanol degradation	71.43	5 of 7
66794	glutamate and glutamine metabolism	71.43	20 of 28
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	glycolysis	70.59	12 of 17
66794	propionate fermentation	70	7 of 10
66794	pyrimidine metabolism	68.89	31 of 45
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	alanine metabolism	65.52	19 of 29
66794	purine metabolism	63.83	60 of 94
66794	C4 and CAM-carbon fixation	62.5	5 of 8
66794	ketogluconate metabolism	62.5	5 of 8
66794	degradation of sugar alcohols	62.5	10 of 16
66794	gluconeogenesis	62.5	5 of 8
66794	lipid metabolism	61.29	19 of 31
66794	4-hydroxyphenylacetate degradation	60	6 of 10
66794	glycogen metabolism	60	3 of 5
66794	methionine metabolism	57.69	15 of 26
66794	ubiquinone biosynthesis	57.14	4 of 7
66794	tetrahydrofolate metabolism	57.14	8 of 14
66794	NAD metabolism	55.56	10 of 18
66794	aspartate and asparagine metabolism	55.56	5 of 9
66794	d-mannose degradation	55.56	5 of 9
66794	cysteine metabolism	55.56	10 of 18
66794	non-pathway related	55.26	21 of 38
66794	histidine metabolism	55.17	16 of 29
66794	lysine metabolism	54.76	23 of 42
66794	arginine metabolism	54.17	13 of 24
66794	leucine metabolism	53.85	7 of 13
66794	urea cycle	53.85	7 of 13
66794	oxidative phosphorylation	51.65	47 of 91
66794	ethanol fermentation	50	1 of 2
66794	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
66794	Entner Doudoroff pathway	50	5 of 10
66794	phenylmercury acetate degradation	50	1 of 2
66794	tryptophan metabolism	50	19 of 38
66794	CMP-KDO biosynthesis	50	2 of 4
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	quinate degradation	50	1 of 2
66794	starch degradation	50	5 of 10
66794	pantothenate biosynthesis	50	3 of 6
66794	isoprenoid biosynthesis	46.15	12 of 26
66794	vitamin B6 metabolism	45.45	5 of 11
66794	metabolism of disaccharids	45.45	5 of 11
66794	phenol degradation	45	9 of 20
66794	4-hydroxymandelate degradation	44.44	4 of 9
66794	glutathione metabolism	42.86	6 of 14
66794	mevalonate metabolism	42.86	3 of 7
66794	tyrosine metabolism	42.86	6 of 14
66794	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
66794	gallate degradation	40	2 of 5
66794	3-phenylpropionate degradation	40	6 of 15
66794	ethylmalonyl-CoA pathway	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	glycine metabolism	40	4 of 10
66794	dTDPLrhamnose biosynthesis	37.5	3 of 8
66794	androgen and estrogen metabolism	37.5	6 of 16
66794	carotenoid biosynthesis	36.36	8 of 22
66794	dolichyl-diphosphooligosaccharide biosynthesis	36.36	4 of 11
66794	d-xylose degradation	36.36	4 of 11
66794	bile acid biosynthesis, neutral pathway	35.29	6 of 17
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	cyanate degradation	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	nitrate assimilation	33.33	3 of 9
66794	lipid A biosynthesis	33.33	3 of 9
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	degradation of pentoses	32.14	9 of 28
66794	ascorbate metabolism	31.82	7 of 22
66794	sulfate reduction	30.77	4 of 13
66794	phenylpropanoid biosynthesis	30.77	4 of 13
66794	myo-inositol biosynthesis	30	3 of 10
66794	reductive acetyl coenzyme A pathway	28.57	2 of 7
66794	degradation of hexoses	27.78	5 of 18
66794	toluene degradation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	lactate fermentation	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	allantoin degradation	22.22	2 of 9

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
18416	-	-	-	-	-	-	-	-	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
18416	+	-	+	-	-	-	+	-	-	-	-	not determinedn.d.	+	-	-	-	+	-	-	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
18416	not determinedn.d.	+	-	-	-	-	-	-	-	-	+	-	-	-	-	+	-	-	-	-
120502	-	+	-	-	-	+	-	-	-	-	+	+	-	-	-	+	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
120502	not determinedn.d.	+	-	-	-	+/-	-	-	-	-	-	+	+	+	-	-	-	-	-	+	-	+/-	-	-	+	+/-	+	-	+	-	-	+	+	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
120502	not determinedn.d.	+	+	-	+	+	-	-	+	-	+	+	-	-	-	-	-	-	+	-	-	-	-	+	-	-	+	-	-	-	-	-	+	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	+	-	-	-	+	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Host	#Human
#Host Body-Site	#Organ

Isolation

@ref	Sample type
8440	human skin
51329	Human skin
67770	Human skin
120502	Human, Skin

Taxonmaps

69479

Global distribution of 16S sequence X80749 (>99% sequence identity) for Rothia kristinae subclade from Microbeatlas

Aquatic Samples	2439
Soil Samples	2017
Animal Samples	15476
Plant Samples	792
Total Samples	20724

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
8440	yes, in single cases	1	Risk group (German classification) According to TRBA 466
18416		1
120502		1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM1602885v1 assembly for Rothia kristinae FDAARGOS_864	complete	GCA_016028855	37923.27	8120149030	37923	92.83
67770	ASM413656v1 assembly for Rothia kristinae ATCC 27570	contig	GCA_004136565	37923.24	8068415451	37923	70.43
67770	ASM157086v1 assembly for Rothia kristinae NBRC 15354	contig	GCA_001570865	1349768.3	2731957608	1349768	65.97
67770	52087_G01 assembly for Rothia kristinae NCTC11038	contig	GCA_900452535	37923.19	2821764000	37923	26.03

Genome browser: GCA_016028855

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	M.kristinae 16S rDNA	X80749	1475		37923
124043	Rothia kristinae DSM 20032 gene for 16S rRNA, partial sequence.	LC752303	569		37923

GC content

@ref	GC-content (mol%)
8440	66.8
67770	66.8
67770	71.9

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Rothia kristinae NBRC 15354
NCBI: GCA_001570865

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	53.60	no
125439	motility	BacteriaNetⓘ	no	83.50	no
125439	gram_stain	BacteriaNetⓘ	positive	99.10	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.10	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Rothia kristinae strain FDAARGOS_864 strain Not applicable
PATRIC: 37923.27

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	91.73	no
125438	anaerobic	anaerobicⓘ	no	96.75	yes
125438	spore-forming	spore-formingⓘ	no	80.22	no
125438	aerobic	aerobicⓘ	yes	66.69	yes
125438	thermophilic	thermophileⓘ	no	96.50	yes
125438	flagellated	motile2+ⓘ	no	94.00	no

Literature

Title	Authors	Journal	DOI	Year
Fatty Acid Conjugation Leads to Length-Dependent Antimicrobial Activity of a Synthetic Antibacterial Peptide (Pep19-4LF).	Storck P, Umstatter F, Wohlfart S, Domhan C, Kleist C, Werner J, Brandenburg K, Zimmermann S, Haberkorn U, Mier W, Uhl P.	Antibiotics (Basel)	10.3390/antibiotics9120844	2020
Genome-Based Taxonomic Classification of the Phylum Actinobacteria.	Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M.	Front Microbiol	10.3389/fmicb.2018.02007	2018
A Rare Agent in Blood: Rothia kristinae.	Usta O, Copur S, Besli Y, Ergonul O.	Infect Dis Clin Microbiol	10.36519/idcm.2025.436	2025
Healthcare environments in pulmonary rehabilitation units: Effective -infection control through integration of long-term antimicrobial materials.	Chao KY, Chen CY, Liu WL, Wu JS, Huang YT.	Multidiscip Respir Med	10.5826/mrm.2025.1018	2025
Antibiotic Susceptibility Report of Bacteria Isolated From Blood Cultures of Neutropenic Sepsis Patients Undergoing Chemotherapy.	Musbau T, Thinn PP, Paing AMM, Swe TH, Sebastian N, Phyo E, Al-Bubseeree B, Griffith D.	Cureus	10.7759/cureus.85903	2025
Microbial Contamination of Percutaneous Endoscopic Gastrostomy Tube and Nasogastric Tube with Drug-Resistant Bacteria.	Kanamori S, Koya H, Kurata N, Ishino K.	Ann Nutr Metab	10.1159/000543972	2025
Biofilm formation by heat-resistant dairy bacteria: multispecies biofilm model under static and dynamic conditions.	Diarra C, Goetz C, Gagnon M, Roy D, Jean J.	Appl Environ Microbiol	10.1128/aem.00713-23	2023
Pulmonary granulomas and Mendelson syndrome in an immunocompromised patient.	Xu H, Zhang R, Zhang X, Wang H, Xiong Y, Zheng B, Zhang Z, Lin L.	Heliyon	10.1016/j.heliyon.2024.e32256	2024
Bacteriological Identification, Characterization and Changes of Feces Microbiome in Prostate Cancer Patients Undergoing Radiotherapy.	Florkiewicz AB, Fijalkowski P, Fijalkowski P, Zloch M, Ludwiczak A, Gabrys D, Mista W, Mrochem-Kwarciak J, Jedrzejewska A, Telka E, Rabsztyn M, Czeszewska-Rosiak G, Mametov R, Tretyn A, Pomastowski P.	Cancer Manag Res	10.2147/cmar.s517416	2025
Exploring the biosynthetic gene clusters in Brevibacterium: a comparative genomic analysis of diversity and distribution.	Cumsille A, Serna-Cardona N, Gonzalez V, Claverias F, Undabarrena A, Molina V, Salva-Serra F, Moore ERB, Camara B.	BMC Genomics	10.1186/s12864-023-09694-7	2023
New Phage-Derived Antibacterial Enzyme PolaR Targeting Rothia spp.	Miernikiewicz P, Barylski J, Wilczak A, Dragos A, Rybicka I, Baldysz S, Szymczak A, Dogsa I, Rokush K, Harhala MA, Ciekot J, Ferenc S, Gnus J, Witkiewicz W, Dabrowska K.	Cells	10.3390/cells12151997	2023
Extended Reality Head-Mounted Displays Are Likely to Pose a Significant Risk in Medical Settings While Current Classification Remains as Non-Critical.	Goldsworthy A, Olsen M, Koh A, Demaneuf T, Singh G, Almheiri R, Chapman B, Almazrouei S, Ghemrawi R, Senok A, McKirdy S, Alghafri R, Tajouri L.	Microorganisms	10.3390/microorganisms12040815	2024
Microbiological effect of topically applied Weissella cibaria on equine pastern dermatitis.	Stykova E, Valocky I, Kacirova J, Fecskeova LK.	Front Vet Sci	10.3389/fvets.2024.1493756	2024
Isolation and characterization of multiple-stress tolerant bacteria from radon springs.	Timkina E, Kulisova M, Palyzova A, Maresova H, Matatkova O, Rezanka T, Kolouchova IJ.	PLoS One	10.1371/journal.pone.0299532	2024
Presence of Contagious Bacterial Flora in Formalin-Fixed Cadavers: A Potential Health Hazard to Medical Professionals.	Gundreddy P, Gaurkar SS.	Cureus	10.7759/cureus.30684	2022
An optimized culturomics strategy for isolation of human milk microbiota.	Wang F, Yu L, Ren Y, Zhang Q, He S, Zhao M, He Z, Gao Q, Chen J.	Front Microbiol	10.3389/fmicb.2024.1272062	2024
Prebiotic Oligosaccharides in Skin Health: Benefits, Mechanisms, and Cosmetic Applications.	Zeng M, Li Y, Cheng J, Wang J, Liu Q.	Antioxidants (Basel)	10.3390/antiox14060754	2025
High Level of Interaction between Phages and Bacteria in an Artisanal Raw Milk Cheese Microbial Community.	Queiroz LL, Lacorte GA, Isidorio WR, Landgraf M, de Melo Franco BDG, Pinto UM, Hoffmann C.	mSystems	10.1128/msystems.00564-22	2023
Cardiometabolic disease risk in gorillas is associated with altered gut microbial metabolism.	Davison S, Mascellani Bergo A, Ward Z, Sackett A, Strykova A, Jaimes JD, Travis D, Clayton JB, Murphy HW, Danforth MD, Smith BK, Blekhman R, Fuh T, Niatou Singa FS, Havlik J, Petrzelkova K, Gomez A.	NPJ Biofilms Microbiomes	10.1038/s41522-025-00664-3	2025
The Skin Microbiome and Bioactive Compounds: Mechanisms of Modulation, Dysbiosis, and Dermatological Implications.	Wojciechowska K, Dos Santos Szewczyk K.	Molecules	10.3390/molecules30224363	2025
Comparative genomics of Rothia species reveals diversity in novel biosynthetic gene clusters and ecological adaptation to different eukaryotic hosts and host niches.	Oliveira IMF, Ng DYK, van Baarlen P, Stegger M, Andersen PS, Wells JM.	Microb Genom	10.1099/mgen.0.000854	2022
Bacterial microbiota of the contact lens surface and associated care behaviours.	Udomwech L, Karnjana K, Jewboonchu J, Rattanathamma P, Narkkul U, Juhong J, Mordmuang A.	Heliyon	10.1016/j.heliyon.2022.e09038	2022
The Intratumoral Bacterial Metataxonomic Signature of Hepatocellular Carcinoma.	Huang JH, Wang J, Chai XQ, Li ZC, Jiang YH, Li J, Liu X, Fan J, Cai JB, Liu F.	Microbiol Spectr	10.1128/spectrum.00983-22	2022
Marine Actinobacteria a New Source of Antibacterial Metabolites to Treat Acne Vulgaris Disease-A Systematic Literature Review.	De La Hoz-Romo MC, Diaz L, Villamil L.	Antibiotics (Basel)	10.3390/antibiotics11070965	2022
Kocuria koreensis sp. nov., isolated from fermented seafood.	Park EJ, Roh SW, Kim MS, Jung MJ, Shin KS, Bae JW	Int J Syst Evol Microbiol	10.1099/ijs.0.012310-0	2009
Kocuria halotolerans sp. nov., an actinobacterium isolated from a saline soil in China.	Tang SK, Wang Y, Lou K, Mao PH, Xu LH, Jiang CL, Kim CJ, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.006627-0	2009
Kocuria polaris sp. nov., an orange-pigmented psychrophilic bacterium isolated from an Antarctic cyanobacterial mat sample.	Reddy GSN, Prakash JSS, Prabahar V, Matsumoto GI, Stackebrandt E, Shivaji S	Int J Syst Evol Microbiol	10.1099/ijs.0.02336-0	2003
Rothia santali sp. nov., endophytic bacteria isolated from sandalwood (Santalum album L.) seedling.	Tuikhar N, Kirdat K, Nair G, Padma S, Thorat V, Swetha P, Sathe S, Sundararaj R, Yadav A	Arch Microbiol	10.1007/s00203-022-03237-6	2022

References

#8440	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20032
#18416	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the HKI: Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) . Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI):
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#40056	; Curators of the CIP;
#51329	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 33026
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68371	Automatically annotated from API 50CH acid .
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120502	Collection of Institut Pasteur ; Curators of the CIP; CIP 81.69
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Rothia kristinae (DSM 20032, ATCC 27570, CCM 2690)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Compound production

Murein

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Pathway annotation

API 20E

API coryne

API zym

API 50CHac

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_016028855

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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