Citricoccus alkalitolerans (CCUG 51943, CCTCC AA 203008, DSM 15665)

Name: Citricoccus alkalitolerans (CCUG 51943, CCTCC AA 203008, DSM 15665)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7632

Type strain

Culture col. no. CCUG 51943 CCTCC AA 203008 DSM 15665 CCTCC AA 20308 KCTC 19012 3 more

NCBI tax ID(s) 246603

Special Collections DSMZ CCUG JCM KCTC CIP Literature strains

History <- W.-J. Li, YIM 2003, W.J. Li, Yunnan Univ., Kunming, China: strain YIM 70010 KCTC 19012 <-- W.-J. Li YIM 70010. <- W. J. Li CIP <- 2003, W.J. Li, Yunnan Univ., Kunming, China: strain YIM 70010

Links

Citricoccus alkalitolerans CCUG 51943 is an aerobe, Gram-positive, coccus-shaped bacterium that was isolated from soil.

Gram-positive coccus-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 134253

DSM 15665,KCTC 19012,JCM 13012,CIP 107968,CCUG 51943

@ref 20215

Last LPSN update 2026-02-09 11:19:31

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Micrococcaceae

Genus Citricoccus

Species Citricoccus alkalitolerans

Full scientific name Citricoccus alkalitolerans Li et al. 2005

BacDive ID	Other strains from **Citricoccus alkalitolerans** (1)	Type strain
153856	C. alkalitolerans CCUG 51386

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape
31296	positive	0.65 µm	0.65 µm	coccus-shaped
67771	positive
116251	positive			coccus-shaped

Colony morphology

Pigmentation

31296

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
6020	ISP2 MEDIUM (DSMZ Medium 987)	Medium recipe at MediaDive	Name: ISP 2 MEDIUM (DSMZ Medium 987) Composition: Agar 20.0 g/l Malt extract 10.0 g/l Dextrose 4.0 g/l Yeast extract 4.0 g/l Distilled water
40735	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
116251	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
6020	positive	growth	28
31296	positive	growth	10-37
31296	positive	optimum	28
40735	positive	growth	30
59381	positive	growth	30
67770	positive	growth	28
67771	positive	growth	30
116251	positive	growth	10-30
116251	negative	growth	37
116251	negative	growth	41
116251	negative	growth	45

Culture pH

@ref	Ability	Type	PH	PH range
31296	positive	optimum	8.5
31296	positive	growth	5.5-12	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
31296	aerobe
59381	aerobe
67771	aerobe
116251	facultative anaerobe

Spore formation

31296

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
31296	NaCl	positive	growth	0-15 %
31296	NaCl	positive	optimum	2.5 %

Murein

@ref	Murein short key	Type
6020	A11.40	A4alpha L-Lys-Gly-D-Glu

Observation

@ref	Observation
67770	quinones: MK-9(H₂)
67771	quinones: MK-9(H2)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
31296	22599 ChEBI	arabinose	+	carbon source
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
31296	17057 ChEBI	cellobiose	+	carbon source
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	-	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
31296	23652 ChEBI	dextrin	+	carbon source
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
31296	28260 ChEBI	galactose	+	carbon source
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
31296	17234 ChEBI	glucose	+	carbon source
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
31296	15428 ChEBI	glycine	+	carbon source
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
116251	606565 ChEBI	hippurate	+	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
31296	17716 ChEBI	lactose	+	carbon source
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
31296	17306 ChEBI	maltose	+	carbon source
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
31296	29864 ChEBI	mannitol	+	carbon source
31296	37684 ChEBI	mannose	+	carbon source
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
116251	17632 ChEBI	nitrate	-	reduction
116251	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
31296	33942 ChEBI	ribose	+	carbon source
31296	17814 ChEBI	salicin	+	carbon source
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
31296	30911 ChEBI	sorbitol	+	carbon source
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
31296	17992 ChEBI	sucrose	+	carbon source
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid
31296	18222 ChEBI	xylose	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
116251	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
116251	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
116251	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
116251	amylase	+
68382	beta-galactosidase	-	3.2.1.23	from API zym
116251	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
116251	caseinase	-	3.4.21.50
31296	catalase	+	1.11.1.6
116251	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
116251	DNase	+
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
116251	gamma-glutamyltransferase	-	2.3.2.2
116251	gelatinase	+/-
116251	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
116251	lipase	-
68382	lipase (C 14)	-		from API zym
116251	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
116251	ornithine decarboxylase	-	4.1.1.17
116251	oxidase	-
116251	phenylalanine ammonia-lyase	-	4.3.1.24
68382	trypsin	+	3.4.21.4	from API zym
116251	tween esterase	-
116251	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
116251	-	-	+	-	-	+	-	-	+	-	-	+	-	-	-	-	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
116251	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
116251	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
6020	soil	The eastern desert	Egypt	EGY	Africa
67770	Desert soil		Egypt	EGY	Africa
67771	Desert soil		Egypt	EGY	Africa
116251	Environment, Soil	Eastern desert	Egypt	EGY	Africa	2002

Taxonmaps

69479

Global distribution of 16S sequence AY376164 (>99% sequence identity) for Micrococcus from Microbeatlas

Aquatic Samples	94
Soil Samples	425
Animal Samples	352
Plant Samples	78
Total Samples	949

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6020	1	Risk group (German classification) According to TRBA 466
116251	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM4265471v1 assembly for Citricoccus alkalitolerans CCUG 51943	scaffold	GCA_042654715	246603	77.16
124043	ASM3952817v1 assembly for Citricoccus alkalitolerans JCM 13012	scaffold	GCA_039528175	246603	62.5

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
6020	Citricoccus alkalitolerans strain YIM 70010 16S ribosomal RNA gene, partial sequence	AY376164	1480	246603
124043	Citricoccus alkalitolerans strain YIM 70010 16S ribosomal RNA gene, partial sequence.	MW111197	599	246603
124043	Citricoccus alkalitolerans strain YIM 70010 16S ribosomal RNA gene, partial sequence.	PQ524549	600	246603

GC content

@ref	GC-content (mol%)	Method
6020	67.8	high performance liquid chromatography (HPLC)
31296	63.8
67770	67.8
67771	63.8	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Living in a bottle: Bacteria from sediment-associated Mediterranean waste and potential growth on polyethylene terephthalate.	Vidal-Verdu A, Latorre-Perez A, Molina-Menor E, Baixeras J, Pereto J, Porcar M.	Microbiologyopen	10.1002/mbo3.1259	2022
Enzymology	Thermophilic and alkaliphilic Actinobacteria: biology and potential applications.	Shivlata L, Satyanarayana T.	Front Microbiol	10.3389/fmicb.2015.01014	2015
Phylogeny	Citricoccus lacusdiani sp. nov., an actinobacterium promoting Microcystis growth with limited soluble phosphorus.	Zhang BH, Salam N, Cheng J, Xiao M, Li HQ, Yang JY, Zha DM, Li WJ	Antonie Van Leeuwenhoek	10.1007/s10482-016-0745-y	2016
Phylogeny	Citricoccus zhacaiensis sp. nov., isolated from a bioreactor for saline wastewater treatment.	Meng FX, Yang XC, Yu PS, Pan JM, Wang CS, Xu XW, Wu M	Int J Syst Evol Microbiol	10.1099/ijs.0.011635-0	2009
Phylogeny	Citricoccus parietis sp. nov., isolated from a mould-colonized wall and emended description of Citricoccus alkalitolerans Li et al. 2005.	Schafer J, Martin K, Kampfer P	Int J Syst Evol Microbiol	10.1099/ijs.0.012567-0	2009
Phylogeny	Citricoccus alkalitolerans sp. nov., a novel actinobacterium isolated from a desert soil in Egypt.	Li WJ, Chen HH, Zhang YQ, Kim CJ, Park DJ, Lee JC, Xu LH, Jiang CL	Int J Syst Evol Microbiol	10.1099/ijs.0.63237-0	2005

References

#6020	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15665
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#27615	IJSEM 87 2005 ( DOI 10.1099/ijs.0.63237-0 , PubMed 15653858 )
#31296	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27615
#40735	; Curators of the CIP;
#59381	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 51943
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#116251	Collection of Institut Pasteur ; Curators of the CIP; CIP 107968
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Citricoccus alkalitolerans (CCUG 51943, CCTCC AA 203008, DSM 15665)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Murein

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 50CHac

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_042654715

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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