Paenarthrobacter nicotinovorans (DSM 420, ATCC 49919, JCM 3874)

Name: Paenarthrobacter nicotinovorans (DSM 420, ATCC 49919, JCM 3874)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7576

Type strain

Culture col. no. DSM 420 ATCC 49919 JCM 3874 VKM Ac-1988 NCAIM B.02288 8 more

NCBI tax ID(s) 29320

Special Collections DSMZ JCM KCTC CIP Wink compendium

History DSMZ <- H.-J. Knackmuss <- K. Decker JCM <-- DSM 420 <-- H. J. Knackmuss <-- K. Decker. CIP <- 2001, JCM <- DSMZ <- H.J. Knackmuss <- K. Decker

Links

Paenarthrobacter nicotinovorans DSM 420 is an aerobe, Gram-positive, rod-shaped bacterium of the family Micrococcaceae.

Gram-positive rod-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 10200

LMG 16253,ATCC 49919,DSM 420,JCM 3874,IFO 15511,VKM Ac-1988,CIP 106990,KCTC 9902,NBRC 15511,IAM 14587,CGMCC 1.1933

@ref 20215

Last LPSN update 2026-05-29 08:26:41

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Micrococcaceae

Genus Paenarthrobacter

Species Paenarthrobacter nicotinovorans

Full scientific name Paenarthrobacter nicotinovorans (Kodama et al. 1992) Busse 2016

Synonyms (1)

Arthrobacter nicotinovorans

BacDive ID	Other strains from **Paenarthrobacter nicotinovorans** (1)	Type strain
176692	P. nicotinovorans CT11-39, DSM 117385

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
121294	positive	rod-shaped
125438			90.5
125438	positive		90.972

Colony morphology

@ref	Colony color	Incubation period	Medium used	Hemolysis ability
19535	Light ivory	10-14 days	ISP 2
19535	Zinc yellow	10-14 days	ISP 3
19535	Ivory	10-14 days	ISP 4
19535		10-14 days	ISP 5
19535	Golden yellow	10-14 days	ISP 6
19535	Sand yellow	10-14 days	ISP 7
121294				1

Multicellular morphology

@ref	Forms multicellular complex	Medium name
19535		ISP 2
19535		ISP 3
19535		ISP 4
19535		ISP 5
19535		ISP 6
19535		ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
191	CORYNEBACTERIUM AGAR (DSMZ Medium 53)	Medium recipe at MediaDive	Name: CORYNEBACTERIUM AGAR (DSMZ Medium 53) Composition: Agar 15.0 g/l Casein peptone 10.0 g/l NaCl 5.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled water
19535	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19535	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19535	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19535	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19535	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19535	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
41902	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
121294	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
191	positive	growth	30
19535	positive	optimum	28
41902	positive	growth	30
67770	positive	growth	30
121294	positive	growth	25-45
121294	negative	growth	10
121294	negative	growth	15

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
121294	aerobe
125438	aerobe	92.162
125439	facultative anaerobe	91.2

Spore formation

@ref	Spore formation	Confidence
125439		91.44

Compound production

191

Compoundnicotine blue

Halophily

@ref	Salt	Growth	Tested relation	Concentration
19535	NaCl	positive	maximum	5 %
121294	NaCl	positive	growth	0-10 %

Murein

@ref	Murein short key	Type
191	A11.17	A3alpha L-Lys-L-Ala-L-Thr-L-Ala

Observation

67770

Observationquinones: MK-9(H₂)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
19535	22599 ChEBI	arabinose	+
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
19535	62968 ChEBI	cellulose	-
121294	16947 ChEBI	citrate	-	carbon source
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
68379	17634 ChEBI	D-glucose	+	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	+	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	+	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	-	hydrolysis	from API Coryne
121294	4853 ChEBI	esculin	-	hydrolysis
19535	28757 ChEBI	fructose	+
68379	5291 ChEBI	gelatin	+	hydrolysis	from API Coryne
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
19535	17234 ChEBI	glucose	+
68379	28087 ChEBI	glycogen	+	fermentation	from API Coryne
121294	606565 ChEBI	hippurate	+	hydrolysis
68379	17716 ChEBI	lactose	+	fermentation	from API Coryne
68368	25094 ChEBI	lysine	-	degradation	from API 20E
68379	17306 ChEBI	maltose	+	fermentation	from API Coryne
19535	29864 ChEBI	mannitol	+
19535	17268 ChEBI	myo-inositol	+
68379	17632 ChEBI	nitrate	+	reduction	from API Coryne
121294	17632 ChEBI	nitrate	+	reduction
121294	17632 ChEBI	nitrate	+	respiration
121294	16301 ChEBI	nitrite	-	reduction
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
19535	16634 ChEBI	raffinose	+
19535	26546 ChEBI	rhamnose	+
19535	17992 ChEBI	sucrose	+
68379	17992 ChEBI	sucrose	+	fermentation	from API Coryne
121294	35020 ChEBI	tributyrin	+	hydrolysis
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68379	16199 ChEBI	urea	+	hydrolysis	from API Coryne
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E
19535	18222 ChEBI	xylose	-

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
121294	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite
68368	16136 ChEBI	hydrogen sulfide	from API 20E
121294	35581 ChEBI	indole
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test	Voges-proskauer-test	Methylred-test
121294	15688 ChEBI	acetoin		+
121294	17234 ChEBI	glucose			+
68368	35581 ChEBI	indole	-			from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
121294	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68379	alkaline phosphatase	-	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
121294	amylase	-
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
121294	beta-galactosidase	+	3.2.1.23
68379	beta-galactosidase	+	3.2.1.23	from API Coryne
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68379	beta-glucosidase	-	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	+	3.2.1.31	from API Coryne
121294	caseinase	-	3.4.21.50
121294	catalase	+	1.11.1.6
121294	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
121294	gamma-glutamyltransferase	-	2.3.2.2
121294	gelatinase	+
68379	gelatinase	+		from API Coryne
68368	gelatinase	+		from API 20E
121294	lecithinase	+
121294	lipase	+
68382	lipase (C 14)	-		from API zym
121294	lysine decarboxylase	-	4.1.1.18
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68379	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API Coryne
121294	ornithine decarboxylase	-	4.1.1.17
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
121294	oxidase	-
121294	phenylalanine ammonia-lyase	+	4.3.1.24
121294	protease	+
68379	pyrazinamidase	+	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	+	3.4.19.3	from API Coryne
121294	tryptophan deaminase	-
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
121294	tween esterase	-
121294	urease	-	3.5.1.5
68379	urease	+	3.5.1.5	from API Coryne
68368	urease	+	3.5.1.5	from API 20E

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
19535	+	-	-	-	+	-	+	-	-	not determinedn.d.	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
19535	+	+	+	-	+	+	+	+	-	+	+	not determinedn.d.	+	+	-	+	+	+	+	+	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
19535	not determinedn.d.	+	+	+	-	+	+	+	+	-	+	+	+	+	-	+	+	+	+	+
121294	-	+	+	+	-	-	-	-	-	-	+	-	-	-	-	+	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
121294	not determinedn.d.	+	+	+	+	-	-	-	+	-	+	+	+	+	+	-	-	+	-	-	-	-	-	-	-	-	+	-	-	-	-	+	+	-	+	-	+	-	-	-	+	+	+	-	+	-	+	-	-	-	-	-	-	+	-	-	-	-	-	-	+	+	+	-	+	+	-	-	+	+	+	-	-	+	+	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	+	-	+	-

Isolation, sampling and environmental information

Taxonmaps

69479

Global distribution of 16S sequence X80743 (>99% sequence identity) for Paenarthrobacter from Microbeatlas

Aquatic Samples	27
Soil Samples	296
Animal Samples	173
Plant Samples	74
Total Samples	570

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
191	1	Risk group (German classification) According to TRBA 466
19535	1	Hazard group According to TRBA 466
121294	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM2191934v1 assembly for Paenarthrobacter nicotinovorans ATCC 49919	complete	GCA_021919345	29320.60	8046637244	29320	90.86
66792	ASM1787644v1 assembly for Paenarthrobacter nicotinovorans DSM 420	contig	GCA_017876445	29320.24	2918346339	29320	71.02
66792	ASM1464873v1 assembly for Paenarthrobacter nicotinovorans JCM 3874	scaffold	GCA_014648735	29320.11	8112945059	29320	63.41

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	A.nicotinovorans 16S rDNA	X80743	1468	29320
124043	Paenarthrobacter nicotinovorans strain DSM 420 16S ribosomal RNA gene, partial sequence.	OR660285	1153	29320
124043	Paenarthrobacter nicotinovorans strain DSM 420 (T) 16S ribosomal RNA gene, partial sequence.	MK424298	1440	29320
124043	Paenarthrobacter nicotinovorans strain JCM 3874 16S ribosomal RNA gene, partial sequence.	MT760469	1343	29320

GC content

@ref	GC-content (mol%)	Method
191	62.0	high performance liquid chromatography (HPLC)
67770	62.4	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM1787644v1 assembly for Paenarthrobacter nicotinovorans DSM 420
BV-BRC: 29320.24

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	91.20	no
125439	gram_stain	BacteriaNetⓘ	positive	82.86	no
125439	motility	BacteriaNetⓘ	no	89.02	no
125439	spore_formation	BacteriaNetⓘ	no	91.44	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Paenarthrobacter nicotinovorans ATCC 49919
NCBI: GCA_021919345.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	90.97	no
125438	anaerobic	anaerobicⓘ	no	98.41	no
125438	aerobic	aerobicⓘ	yes	92.16	no
125438	spore-forming	spore-formingⓘ	no	60.45	no
125438	thermophilic	thermophileⓘ	no	99.00	yes
125438	flagellated	motile2+ⓘ	no	90.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Characterisation of the Paenarthrobacter nicotinovorans ATCC 49919 genome and identification of several strains harbouring a highly syntenic nic-genes cluster.	El-Sabeh A, Mlesnita AM, Munteanu IT, Honceriu I, Kallabi F, Boiangiu RS, Mihasan M.	BMC Genomics	10.1186/s12864-023-09644-3	2023
Phylogeny	Biosynthetic gene clusters with biotechnological applications in novel Antarctic isolates from Actinomycetota.	Bruna P, Nunez-Montero K, Contreras MJ, Leal K, Garcia M, Abanto M, Barrientos L.	Appl Microbiol Biotechnol	10.1007/s00253-024-13154-x	2024
Genetics	A comparative genomic study of a hydrocarbon-degrading marine bacterial consortium.	Rojas-Vargas J, Rebollar EA, Sanchez-Flores A, Pardo-Lopez L.	PLoS One	10.1371/journal.pone.0303363	2024
	Time-Dependent Analysis of Paenarthrobacter nicotinovorans pAO1 Nicotine-Related Proteome.	Mihasan M, Boiangiu RS, Guzun D, Babii C, Aslebagh R, Channaveerappa D, Dupree E, Darie CC.	ACS Omega	10.1021/acsomega.1c01020	2021
Metabolism	Proteomics based analysis of the nicotine catabolism in Paenarthrobacter nicotinovorans pAO1.	Mihasan M, Babii C, Aslebagh R, Channaveerappa D, Dupree E, Darie CC.	Sci Rep	10.1038/s41598-018-34687-y	2018
	An efficient direct screening system for microorganisms that activate plant immune responses based on plant-microbe interactions using cultured plant cells.	Kurokawa M, Nakano M, Kitahata N, Kuchitsu K, Furuya T.	Sci Rep	10.1038/s41598-021-86560-0	2021
Metabolism	Corynebacterium glutamicum CrtR and Its Orthologs in Actinobacteria: Conserved Function and Application as Genetically Encoded Biosensor for Detection of Geranylgeranyl Pyrophosphate.	Henke NA, Austermeier S, Grothaus IL, Gotker S, Persicke M, Peters-Wendisch P, Wendisch VF.	Int J Mol Sci	10.3390/ijms21155482	2020
	Microbial occurrence and antibiotic resistance in ready-to-go food items.	Cole ML, Singh OV.	J Food Sci Technol	10.1007/s13197-018-3180-4	2018
	Insights into Pharmacological Activities of Nicotine and 6-Hydroxy-L-nicotine, a Bacterial Nicotine Derivative: A Systematic Review.	Boiangiu RS, Brinza I, Honceriu I, Mihasan M, Hritcu L.	Biomolecules	10.3390/biom14010023	2023
	Insight Into the Diversity and Possible Role of Plasmids in the Adaptation of Psychrotolerant and Metalotolerant Arthrobacter spp. to Extreme Antarctic Environments.	Romaniuk K, Golec P, Dziewit L.	Front Microbiol	10.3389/fmicb.2018.03144	2018
	Paenarthrobacter sp. GOM3 Is a Novel Marine Species With Monoaromatic Degradation Relevance.	Rosas-Diaz J, Escobar-Zepeda A, Adaya L, Rojas-Vargas J, Cuervo-Amaya DH, Sanchez-Reyes A, Pardo-Lopez L.	Front Microbiol	10.3389/fmicb.2021.713702	2021
Enzymology	Role of glutamate 292 and lysine 331 in catalysis for the flavoenzyme (S)-6-hydroxynicotine oxidase from Shinella sp. HZN7.	Zhang Z, Freeland K, Stull F.	Arch Biochem Biophys	10.1016/j.abb.2025.110492	2025
	Synergistic Treatment of Reverse Osmosis Membrane Biofouling with Quorum Quenching Bacteria and Hitchhiking Phages.	Wang X, Ruan C, Shen C, Liao J, Wang D, Alvarez PJJ, Yu P.	Environ Sci Technol	10.1021/acs.est.4c12852	2025
	Toxicity of secondary metabolites of Paenarthrobacter nicotinovorans.	Kakol J, Vang M, Sausen D, Steeno T, Kolokithas A.	MicroPubl Biol	10.17912/micropub.biology.000923	2023
	Rapid arsenite oxidation by Paenarthrobacter nicotinovorans strain SSBW5: unravelling the role of GlpF, aioAB and aioE genes.	Mujawar SY, Shamim K, Vaigankar DC, Naik MM, Dubey SK.	Arch Microbiol	10.1007/s00203-023-03673-y	2023
	Antimicrobial activity of Paenarthrobacter nicotinovorans.	Kakol J, Vang M, Sausen D, Steeno T, Kolokithas A.	MicroPubl Biol	10.17912/micropub.biology.000921	2023
	Time course study of Paenarthrobacter nicotinovorans secondary metabolite toxicity profile.	Kakol J, Vang M, Sausen D, Steeno T, Kolokithas A.	MicroPubl Biol	10.17912/micropub.biology.000922	2023
	Extracellular Synthesis of Bioactive Silver Nanoparticles Using Brevibacillus sp. MAHUQ-41 and Their Potential Application Against Drug-Resistant Bacterial Pathogens Listeria monocytogenes and Yersinia enterocolitica.	Huq MA.	J Funct Biomater	10.3390/jfb16070241	2025
Metabolism	Exploration of Nicotine Metabolism in Paenarthrobacter nicotinovorans pAO1 by Microbial Proteomics.	Mihasan M, Babii C, Aslebagh R, Channaveerappa D, Dupree EJ, Darie CC.	Adv Exp Med Biol	10.1007/978-3-030-15950-4_30	2019
Transcriptome	Macrotranscriptomics analysis for decoding the role of Klebsiella variicola H8 in aroma compound biosynthesis during fermentation of reconstituted tobacco leaf concentrate.	Feng Y, Qi W, Yang J, Liu W, Yang Z, Wang K, Mao D, Huang S, Zhang T.	Front Bioeng Biotechnol	10.3389/fbioe.2025.1635651	2025
Enzymology	A soil bacterial catabolic pathway on the move: Transfer of nicotine catabolic genes between Arthrobacter genus megaplasmids and invasion by mobile elements.	Brandsch R, Mihasan M.	J Biosci	10.1007/s12038-020-00030-9	2020
	Bacterial Mediated Rapid and Facile Synthesis of Silver Nanoparticles and Their Antimicrobial Efficacy against Pathogenic Microorganisms.	Huq MA, Akter S.	Materials (Basel)	10.3390/ma14102615	2021
	Culture-dependent and -independent analyses reveal unique community structure and function in the external mycelial cortices of Ophiocordyceps sinensis.	Li N, Li J, Feng Z, Wu Z, Gao Q, Wang J, Zhang Y, Chen SL, Xing R.	BMC Microbiol	10.1186/s12866-025-03793-z	2025
Genetics	Genomic and Metagenomic Insights into the Distribution of Nicotine-degrading Enzymes in Human Microbiota.	Guan Y, Zhu Z, Peng Q, Li M, Li X, Yang JW, Lu YH, Wang M, Xie BB.	Curr Genomics	10.2174/0113892029302230240319042208	2024
	Modulation in Biofertilization and Biofortification of Wheat Crop by Inoculation of Zinc-Solubilizing Rhizobacteria.	Yadav RC, Sharma SK, Varma A, Rajawat MVS, Khan MS, Sharma PK, Malviya D, Singh UB, Rai JP, Saxena AK.	Front Plant Sci	10.3389/fpls.2022.777771	2022
	Isolation and identification of mycorrhizal helper bacteria of Vaccinium uliginosum and their interaction with mycorrhizal fungi.	Yang Z, Dong H, Zhang S, Jiang J, Zhu H, Yang H, Li L.	Front Microbiol	10.3389/fmicb.2023.1180319	2023
	Neuroprotective Potential of Guiera senegalensis (Combretaceae) Leaf Hydroethanolic Extract against Cholinergic System Dysfunctions and Oxidative Stress in Scopolamine-Induced Cognitive Impairment in Zebrafish (Danio rerio).	Damo JLK, Boiangiu RS, Brinza I, Kenko Djoumessi LB, Rebe RN, Kamleu BN, Guedang SDN, Camdi GW, Bouvourne P, Keugong EW, Ngatanko HHA, Cioanca O, Hancianu M, Foyet HS, Hritcu L.	Plants (Basel)	10.3390/plants11091149	2022
	Angelica purpurascens (Avé-Lall.) Gilli. Essential Oil Improved Brain Function via Cholinergic Modulation and Antioxidant Effects in the Scopolamine-Induced Zebrafish (Danio rerio) Model.	Boiangiu RS, Bagci E, Dumitru G, Hritcu L, Todirascu-Ciornea E.	Plants (Basel)	10.3390/plants11081096	2022
	Endophytic PGPR from Tomato Roots: Isolation, In Vitro Characterization and In Vivo Evaluation of Treated Tomatoes (Solanum lycopersicum L.).	Cochard B, Giroud B, Crovadore J, Chablais R, Arminjon L, Lefort F.	Microorganisms	10.3390/microorganisms10040765	2022
	Cultivable microbial diversity in speleothems using MALDI-TOF spectrometry and DNA sequencing from Krem Soitan, Krem Lawbah, Krem Mawpun, Khasi Hills, Meghalaya, India.	Mudgil D, Paul D, Baskar S, Baskar R, Shouche YS.	Arch Microbiol	10.1007/s00203-022-02916-8	2022
Metabolism	Cotinine Hydroxylase CotA Initiates Biodegradation of Wastewater Micropollutant Cotinine in Nocardioides sp. Strain JQ2195.	Zhao L, Zhao Z, Zhang K, Zhang X, Xu S, Liu J, Liu B, Hong Q, Qiu J, He J.	Appl Environ Microbiol	10.1128/aem.00923-21	2021
Transcriptome	Comprehensive insights into the mechanism of keratin degradation and exploitation of keratinase to enhance the bioaccessibility of soybean protein.	Zhou B, Guo Y, Xue Y, Ji X, Huang Y.	Biotechnol Biofuels Bioprod	10.1186/s13068-023-02426-9	2023
	Bacteriogenic synthesis of morphologically diverse silver nanoparticles and their assessment for methyl orange dye removal and antimicrobial activity.	Patel B, Yadav VK, Desai R, Patel S, Amari A, Choudhary N, Osman H, Patel R, Balram D, Lian KY, Sahoo DK, Patel A.	PeerJ	10.7717/peerj.17328	2024
	Silver Nanoparticles of Artemisia sieberi Extracts: Chemical Composition and Antimicrobial Activities.	Al-Otibi F, Alshammry NA, Alharbi RI, Bin-Jumah MN, AlSubaie MM.	Plants (Basel)	10.3390/plants12112093	2023
Metabolism	Conversion of levoglucosan into glucose by the coordination of four enzymes through oxidation, elimination, hydration, and reduction.	Kuritani Y, Sato K, Dohra H, Umemura S, Kitaoka M, Fushinobu S, Yoshida N.	Sci Rep	10.1038/s41598-020-77133-8	2020
	Green Extracellular Synthesis of Silver Nanoparticles by Pseudomonas alloputida, Their Growth and Biofilm-Formation Inhibitory Activities and Synergic Behavior with Three Classical Antibiotics.	Pernas-Pleite C, Conejo-Martinez AM, Marin I, Abad JP.	Molecules	10.3390/molecules27217589	2022
	Biochemical and Genetic Analysis of 4-Hydroxypyridine Catabolism in Arthrobacter sp. Strain IN13.	Vaitekunas J, Gasparaviciute R, Stankeviciute J, Urbelis G, Meskys R.	Microorganisms	10.3390/microorganisms8060888	2020
Metabolism	Additional Role of Nicotinic Acid Hydroxylase for the Transformation of 3-Succinoyl-Pyridine by Pseudomonas sp. Strain JY-Q.	Li J, Li S, Xie L, Chen G, Shen M, Pan F, Shu M, Yang Y, Jiao Y, Zhang F, Linhardt RJ, Zhong W.	Appl Environ Microbiol	10.1128/aem.02740-20	2021
Metabolism	Characterization, cytotoxicity, and genotoxicity properties of novel biomediated nanosized-silver by Egyptian Streptomyces roseolus for safe antimicrobial applications.	Elnady A, Sorour NM, Abbas RN.	World J Microbiol Biotechnol	10.1007/s11274-022-03231-6	2022
	Anxiolytic, Promnesic, Anti-Acetylcholinesterase and Antioxidant Effects of Cotinine and 6-Hydroxy-L-Nicotine in Scopolamine-Induced Zebrafish (Danio rerio) Model of Alzheimer's Disease.	Boiangiu RS, Mihasan M, Gorgan DL, Stache BA, Hritcu L.	Antioxidants (Basel)	10.3390/antiox10020212	2021
	Green Metallic Nanoparticles: Biosynthesis to Applications.	Chopra H, Bibi S, Singh I, Hasan MM, Khan MS, Yousafi Q, Baig AA, Rahman MM, Islam F, Emran TB, Cavalu S.	Front Bioeng Biotechnol	10.3389/fbioe.2022.874742	2022
Pathogenicity	Cotinine and 6-Hydroxy-L-Nicotine Reverses Memory Deficits and Reduces Oxidative Stress in Abeta_25-35-Induced Rat Model of Alzheimer's Disease.	Boiangiu RS, Mihasan M, Gorgan DL, Stache BA, Petre BA, Hritcu L.	Antioxidants (Basel)	10.3390/antiox9080768	2020
Metabolism	Insights into a dual function amide oxidase/macrocyclase from lankacidin biosynthesis.	Dorival J, Risser F, Jacob C, Collin S, Drager G, Paris C, Chagot B, Kirschning A, Gruez A, Weissman KJ.	Nat Commun	10.1038/s41467-018-06323-w	2018
Biotechnology	EstDZ3: A New Esterolytic Enzyme Exhibiting Remarkable Thermostability.	Zarafeta D, Szabo Z, Moschidi D, Phan H, Chrysina ED, Peng X, Ingham CJ, Kolisis FN, Skretas G.	Front Microbiol	10.3389/fmicb.2016.01779	2016
Genetics	Complete Genome Sequences of Two Closely Related Paenarthrobacter nicotinovorans Strains.	El-Sabeh A, Honceriu I, Kallabi F, Boiangiu RS, Mihasan M	Microbiol Resour Announc	10.1128/mra.00133-22	2022
Metabolism	Identification of large linear plasmids in Arthrobacter spp. encoding the degradation of quinaldine to anthranilate.	Overhage J, Sielker S, Homburg S, Parschat K, Fetzner S	Microbiology (Reading)	10.1099/mic.0.27521-0	2005
Phylogeny	Arthrobacter bambusae sp. nov., isolated from soil of a bamboo grove.	Park Y, Kook M, Ngo HTT, Kim KY, Park SY, Mavlonov GT, Yi TH	Int J Syst Evol Microbiol	10.1099/ijs.0.064550-0	2014
Phylogeny	Reclassification of two strains of Arthrobacter oxydans and proposal of Arthrobacter nicotinovorans sp. nov.	Kodama Y, Yamamoto H, Amano N, Amachi T	Int J Syst Bacteriol	10.1099/00207713-42-2-234	1992

References

#191	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 420
#19535	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#41902	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#121294	Collection of Institut Pasteur ; Curators of the CIP; CIP 106990
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Paenarthrobacter nicotinovorans (DSM 420, ATCC 49919, JCM 3874)

Name and taxonomic classification

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Multicellular morphology

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API 20E

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Genome browser: GCA_021919345

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