Name: Rhizobium viscosum B-1973
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7573

Type strain:

Species: Rhizobium viscosum

Strain Designation: B-1973

Culture col. no.: DSM 7307, ATCC 19584, CECT 908, LMG 16473, NCIMB 9729, NRRL B-1973, JCM 11566, BCRC 14858, CIP 82.105, IAM 14871, KCTC 3388, LMG 17310, MTCC 22, NCFB 2326, PCM 2469, NCDO 2326

Strain history: CIP <- 1982, ATCC <- NRRL: strain B-1973

NCBI tax ID(s): 1673 (species)

SI-ID 8194

LMG 16473, ATCC 19584, LMG 17310, NCFB 2326, NCIMB 9729, NRRL B-1973, JCM 11566, NCDO 2326, CECT 908, KCTC 3388, BCRC 14858, CCRC 14858, IAM 14871, CDBB 20, CIP 82.105, DSM 7307

Other strains from Rhizobium viscosum

R. viscosum DSM 7287, ATCC 19583, NCIMB 9728, ...
R. viscosum CIP 102695, NCDO 2327, NCIMB 10617

Section

Rhizobium viscosum B-1973 is a mesophilic, Gram-negative bacterium that produces polysaccharides and was isolated from soil.

polysaccharide production
Gram-negative
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Rhizobiaceae*
	Genus	*Rhizobium*
	Species	**Rhizobium viscosum**
	Full Scientific Name (LPSN)	Rhizobium viscosum (Gasdorf et al. 1965) Flores-Félix et al. 2017

Synonym

Arthrobacter viscosus

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.978

[Ref.: #20200]	Colony color	Ochre brown (8001)
[Ref.: #20200]	Incubation period	10-14 days
[Ref.: #20200]	Cultivation medium used	ISP 2

[Ref.: #20200]	Colony color	Cream (9001)
[Ref.: #20200]	Incubation period	10-14 days
[Ref.: #20200]	Cultivation medium used	ISP 3

[Ref.: #20200]	Colony color	Light ivory (1015)
[Ref.: #20200]	Incubation period	10-14 days
[Ref.: #20200]	Cultivation medium used	ISP 5

[Ref.: #20200]	Colony color	Colorless
[Ref.: #20200]	Incubation period	10-14 days
[Ref.: #20200]	Cultivation medium used	ISP 6

[Ref.: #20200]	Colony color	Ivory (1014)
[Ref.: #20200]	Incubation period	10-14 days
[Ref.: #20200]	Cultivation medium used	ISP 7

[Ref.: #3109]	Incubation period	3-7 days

[Ref.: #20200]	Multicellular complex forming ability	no
[Ref.: #20200]	Medium Name (multicellularity)	ISP 2

[Ref.: #20200]	Multicellular complex forming ability	no
[Ref.: #20200]	Medium Name (multicellularity)	ISP 3

[Ref.: #20200]	Multicellular complex forming ability	no
[Ref.: #20200]	Medium Name (multicellularity)	ISP 5

[Ref.: #20200]	Multicellular complex forming ability	no
[Ref.: #20200]	Medium Name (multicellularity)	ISP 6

[Ref.: #20200]	Multicellular complex forming ability	no
[Ref.: #20200]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #20200]

Culture medium

ISP 2

[Ref.: #20200]

Culture medium growth

[Ref.: #20200]

Culture medium composition

expand / minimize

[Ref.: #20200]

Culture medium

ISP 3

[Ref.: #20200]

Culture medium growth

[Ref.: #20200]

Culture medium composition

expand / minimize

[Ref.: #20200]

Culture medium

ISP 5

[Ref.: #20200]

Culture medium growth

[Ref.: #20200]

Culture medium composition

expand / minimize

[Ref.: #20200]

Culture medium

ISP 6

[Ref.: #20200]

Culture medium growth

[Ref.: #20200]

Culture medium composition

expand / minimize

[Ref.: #20200]

Culture medium

ISP 7

[Ref.: #20200]

Culture medium growth

[Ref.: #20200]

Culture medium composition

expand / minimize

[Ref.: #39712]

Culture medium

MEDIUM 112 - for Janibacter

[Ref.: #39712]

Culture medium growth

[Ref.: #39712]

Culture medium composition

expand / minimize

[Ref.: #3109]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #3109]

Culture medium growth

[Ref.: #3109]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3109]

Culture medium composition

expand / minimize

[Ref.: #3109]

Culture medium

UNIVERSAL MEDIUM FOR YEASTS (YM) (DSMZ Medium 186)

[Ref.: #3109]

Culture medium growth

[Ref.: #3109]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3109]

Culture medium composition

expand / minimize

[Ref.: #123874]

Culture medium

CIP Medium 112

[Ref.: #123874]

Culture medium growth

[Ref.: #123874]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #3109]	growth	28 °C
[Ref.: #20200]	optimum	28 °C
[Ref.: #39712]	growth	30 °C
[Ref.: #43259]	growth	37 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #3109]	Temperature range	mesophilic
[Ref.: #20200]	Temperature range	mesophilic
[Ref.: #39712]	Temperature range	mesophilic
[Ref.: #43259]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43259]			growth	5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.983

[Ref.: #3109]

Name of produced compound

polysaccharide

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #20200]		NaCl		maximum	7.5	%
[Ref.: #43259]		NaCl		growth	0.5-1.0	%(w/v)

[Ref.: #67770]	Observation	Production of Viscous extracellular polysaccharide
[Ref.: #67770]	Observation	quinones: Q-10

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43259]	2-oxogluconate	-	assimilation
[Ref.: #43259]	3-hydroxybenzoate ChEBI	-	assimilation
[Ref.: #43259]	3-hydroxybutyrate ChEBI	-	assimilation
[Ref.: #43259]	4-hydroxybenzoate ChEBI	-	assimilation
[Ref.: #43259]	5-dehydro-D-gluconate ChEBI	-	assimilation
[Ref.: #43259]	acetate ChEBI	-	assimilation
[Ref.: #43259]	adipate ChEBI	-	assimilation
[Ref.: #20200]	arabinose ChEBI	+
[Ref.: #20200]	cellulose ChEBI	+
[Ref.: #43259]	citrate ChEBI	-	assimilation
[Ref.: #43259]	D-ribose ChEBI	+	assimilation
[Ref.: #43259]	decanoate ChEBI	-	assimilation
[Ref.: #43259]	esculin ChEBI	+	hydrolysis
[Ref.: #20200]	fructose ChEBI	+
[Ref.: #43259]	gluconate ChEBI	-	assimilation
[Ref.: #20200]	glucose ChEBI	+
[Ref.: #43259]	glucose ChEBI	+	assimilation
[Ref.: #43259]	glycogen ChEBI	-	assimilation
[Ref.: #43259]	itaconate ChEBI	-	assimilation
[Ref.: #43259]	L-alanine ChEBI	+	assimilation
[Ref.: #43259]	L-arabinose ChEBI	+	assimilation
[Ref.: #43259]	L-fucose ChEBI	+	assimilation
[Ref.: #43259]	L-histidine ChEBI	+	assimilation
[Ref.: #43259]	L-proline ChEBI	+	assimilation
[Ref.: #43259]	L-rhamnose ChEBI	+	assimilation
[Ref.: #43259]	L-serine ChEBI	-	assimilation
[Ref.: #43259]	L-sorbose ChEBI	+	assimilation
[Ref.: #43259]	lactate ChEBI	+	assimilation
[Ref.: #43259]	malate ChEBI	+	assimilation
[Ref.: #43259]	malonate ChEBI	-	assimilation
[Ref.: #43259]	maltose ChEBI	+	assimilation
[Ref.: #20200]	mannitol ChEBI	+
[Ref.: #43259]	mannitol ChEBI	+	assimilation
[Ref.: #43259]	mannose ChEBI	+	assimilation
[Ref.: #43259]	melibiose ChEBI	+	assimilation
[Ref.: #20200]	myo-inositol ChEBI	+
[Ref.: #43259]	myo-inositol ChEBI	+	assimilation
[Ref.: #43259]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #43259]	nitrate ChEBI	+	reduction
[Ref.: #43259]	phenylacetate ChEBI	-	assimilation
[Ref.: #43259]	propionate ChEBI	-	assimilation
[Ref.: #20200]	raffinose ChEBI	+
[Ref.: #20200]	rhamnose ChEBI	+
[Ref.: #43259]	salicin ChEBI	+	assimilation
[Ref.: #43259]	suberic acid ChEBI	-	assimilation
[Ref.: #20200]	sucrose ChEBI	+
[Ref.: #43259]	sucrose ChEBI	+	assimilation
[Ref.: #43259]	urea ChEBI	+	hydrolysis
[Ref.: #43259]	valerate ChEBI	-	assimilation
[Ref.: #20200]	xylose ChEBI	+
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43259]	ampicillin ChEBI				yes	2	µg
[Ref.: #43259]	cefuroxime ChEBI	yes	30	µg
[Ref.: #43259]	ciprofloxacin ChEBI	yes	5	µg
[Ref.: #43259]	cloxacillin ChEBI				yes	1	µg
[Ref.: #43259]	erythromycin ChEBI				yes	2	µg
[Ref.: #43259]	gentamicin ChEBI	yes	10	µg
[Ref.: #43259]	neomycin ChEBI	yes	5	µg
[Ref.: #43259]	oxytetracycline ChEBI	yes	30	µg
[Ref.: #43259]	penicillin ChEBI				yes	11	Unit
[Ref.: #43259]	polymyxin b ChEBI				yes	300	Unit

	Metabolite production	Metabolite	Production
[Ref.: #43259]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43259]	acid phosphatase	+/-	3.1.3.2
[Ref.: #43259]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43259]	alpha-arabinosidase	+	3.2.1.55
[Ref.: #43259]	alpha-fucosidase	+	3.2.1.51
[Ref.: #43259]	alpha-galactosidase	+	3.2.1.22
[Ref.: #43259]	alpha-glucosidase	+	3.2.1.20
[Ref.: #43259]	alpha-L-rhamnosidase	+	3.2.1.40
[Ref.: #43259]	alpha-maltosidase	-
[Ref.: #43259]	alpha-mannosidase	+	3.2.1.24
[Ref.: #43259]	alpha-xylosidase	-
[Ref.: #43259]	arginine dihydrolase	-	3.5.3.6
[Ref.: #43259]	beta-cellobiase	+/-
[Ref.: #43259]	beta-D-fucosidase	+	3.2.1.38
[Ref.: #43259]	beta-galactosaminidase	+
[Ref.: #43259]	beta-galactosidase	+	3.2.1.23
[Ref.: #43259]	beta-glucosaminidase	+
[Ref.: #43259]	beta-glucosidase	+	3.2.1.21
[Ref.: #43259]	beta-L-arabinosidase	+	3.2.1.88
[Ref.: #43259]	beta-maltosidase	+/-
[Ref.: #43259]	beta-mannosidase	+	3.2.1.25
[Ref.: #43259]	beta-xylosidase	+
[Ref.: #3109]	catalase	+	1.11.1.6
[Ref.: #3109]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #43259]	galacturonidase	-
[Ref.: #43259]	gelatinase	-
[Ref.: #43259]	glucuronidase	-
[Ref.: #43259]	lactosidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3109]	Sample type/isolated from	soil
[Ref.: #3109]	Geographic location (country and/or sea, region)	City Airport
[Ref.: #3109]	Country	Guatemala
[Ref.: #3109]	Country ISO 3 Code	GTM
[Ref.: #3109]	Continent	Middle and South America

[Ref.: #43259]	Sample type/isolated from	soil samples
[Ref.: #43259]	Geographic location (country and/or sea, region)	Guatemala
[Ref.: #43259]	Country	Guatemala
[Ref.: #43259]	Country ISO 3 Code	GTM
[Ref.: #43259]	Continent	Middle and South America

[Ref.: #67770]	Sample type/isolated from	Soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	Guatemala City
[Ref.: #67770]	Country	Guatemala
[Ref.: #67770]	Country ISO 3 Code	GTM
[Ref.: #67770]	Continent	North America

[Ref.: #123874]	Sample type/isolated from	Environment, Soil
[Ref.: #123874]	Country	Guatemala
[Ref.: #123874]	Country ISO 3 Code	GTM
[Ref.: #123874]	Continent	North America
[Ref.: #123874]	Isolation date	1956
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ639832 (>99% sequence identity) for Rhizobium from Microbeatlas

Aquatic Samples	181
Soil Samples	1145
Animal Samples	198
Plant Samples	366
Total Samples	1890

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #20200]	Biosafety level	1
[Ref.: #3109]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123874]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #3109]	Arthrobacter viscosus partial 16S rRNA gene, type strain LMG 16473T	AJ639832	1424	ENA	1673 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Rhizobium viscosum DSM 7307	GCA_014873945	contig	GenBank	1673 tax ID	*
[Ref.: #66792]	Rhizobium viscosum strain DSM 7307	1673.4	wgs	PATRIC	1673 tax ID	*
[Ref.: #66792]	Rhizobium viscosum DSM 7307	2870861536	draft	JGI IMG/M	1673 tax ID	*

[Ref.: #3109]

GC-content

61.5 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Rhizobium viscosum strain DSM 7307 PATRIC: 1673.4
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Rhizobium viscosum strain DSM 7307 PATRIC: 1673.4
[Ref.: #69480]	motile	yes	86.664	no
[Ref.: #69480]	gram-positive	no	97.986	no
[Ref.: #69480]	anaerobic	no	98.786	no
[Ref.: #69480]	aerobic	yes	94.49	no
[Ref.: #69480]	halophile	no	92.832	yes
[Ref.: #69480]	spore-forming	no	94.405	no
[Ref.: #69480]	thermophile	no	99.434	no
[Ref.: #69480]	glucose-util	yes	90.365	no
[Ref.: #69480]	flagellated	no	77.049	no
[Ref.: #69480]	glucose-ferment	no	88.889	no

Availability in culture collections External links

[Ref.: #3109]

Culture collection no.

DSM 7307, ATCC 19584, CECT 908, LMG 16473, NCIMB 9729, NRRL B-1973, JCM 11566, BCRC 14858, CIP 82.105, IAM 14871, KCTC 3388, LMG 17310, MTCC 22, NCFB 2326, PCM 2469, NCDO 2326

[Ref.: #77018]

SI-ID 8194

Literature:

Topic	Title	Authors	Journal	DOI	Year
Enzymology	The biopolymer produced by Rhizobium viscosum CECT 908 is a promising agent for application in microbial enhanced oil recovery.	Couto MR, Gudina EJ, Ferreira D, Teixeira JA, Rodrigues LR	N Biotechnol	10.1016/j.nbt.2018.11.002	2018	*
Phylogeny	Reclassification of Arthrobacter viscosus as Rhizobium viscosum comb. nov.	Flores-Felix JD, Ramirez-Bahena MH, Salazar S, Peix A, Velazquez E	Int J Syst Evol Microbiol	10.1099/ijsem.0.001864	2017	*
	Heteropolysaccharide Formation by Arthrobacter viscosus Grown on Xylose and Xylose Oligosaccharides.	Novak JS, Tanenbaum SW, Nakas JP	Appl Environ Microbiol	10.1128/aem.58.11.3501-3507.1992	1992	*
	Polymer-Producing Species of Arthrobacter.	Gasdorf HJ, Benedict RG, Cadmus MC, Anderson RF, Jackson RW	J Bacteriol	10.1128/jb.90.1.147-150.1965	1965	*

References

#3109

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7307

#20200

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#39712 ; Curators of the CIP;

#43259

José David Flores-Félix, Martha Helena Ramírez-Bahena, Sergio Salazar, Alvaro Peix and Encarna Velázquez: Reclassification of Arthrobacter viscosus as Rhizobium viscosum comb. nov. IJSEM 67: 1789 - 1792 2017 ( DOI 10.1099/ijsem.0.001864 , PubMed 28598309 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#77018

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID8194.1 )

#123874 Collection of Institut Pasteur ; Curators of the CIP; CIP 82.105
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Rhizobium viscosum in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Rhizobium viscosum strain DSM 7307

Rhizobium viscosum strain DSM 7307

Change proposal

Change proposal