Name: Microcella frigidaquae CW1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7506

Type strain:

Species: Microcella frigidaquae

Strain Designation: CW1

Culture col. no.: DSM 23889, JCM 14730, KCTC 13142

Strain history: <- CN Seong, Sunchon Natl. Univ.

NCBI tax ID(s): 424758 (species)

SI-ID 402936

DSM 23889

Section

Microcella frigidaquae CW1 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from cooling water.

Gram-positive
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Microbacteriaceae*
	Genus	*Microcella*
	Species	**Microcella frigidaquae**
	Full Scientific Name (LPSN)	Microcella frigidaquae (Baik et al. 2010) Xie et al. 2022

Synonym

Chryseoglobus frigidaquae

Information on morphological and physiological properties Morphology

[Ref.: #29577]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #29577]	Cell shape	rod-shaped

[Ref.: #29577]	Motility	yes

[Ref.: #21377]	Colony color	Sand yellow (1002)
[Ref.: #21377]	Incubation period	10-14 days
[Ref.: #21377]	Cultivation medium used	ISP 2

[Ref.: #21377]	Colony color	Sand yellow (1002)
[Ref.: #21377]	Incubation period	10-14 days
[Ref.: #21377]	Cultivation medium used	ISP 3

[Ref.: #21377]	Colony color	Broom yellow (1032)
[Ref.: #21377]	Incubation period	10-14 days
[Ref.: #21377]	Cultivation medium used	ISP 4

[Ref.: #21377]	Incubation period	10-14 days
[Ref.: #21377]	Cultivation medium used	ISP 5

[Ref.: #21377]	Incubation period	10-14 days
[Ref.: #21377]	Cultivation medium used	ISP 7

[Ref.: #21377]	Incubation period	10-14 days
[Ref.: #21377]	Cultivation medium used	Suter with tyrosine

[Ref.: #21377]	Incubation period	10-14 days
[Ref.: #21377]	Cultivation medium used	Suter without tyrosine

[Ref.: #29577]

Pigment production

yes

[Ref.: #21377]	Multicellular complex forming ability	no
[Ref.: #21377]	Medium Name (multicellularity)	ISP 2

[Ref.: #21377]	Multicellular complex forming ability	no
[Ref.: #21377]	Medium Name (multicellularity)	ISP 3

[Ref.: #21377]	Multicellular complex forming ability	no
[Ref.: #21377]	Medium Name (multicellularity)	ISP 4

[Ref.: #21377]	Multicellular complex forming ability	no
[Ref.: #21377]	Medium Name (multicellularity)	ISP 5

[Ref.: #21377]	Multicellular complex forming ability	no
[Ref.: #21377]	Medium Name (multicellularity)	ISP 7

[Ref.: #21377]	Multicellular complex forming ability	no
[Ref.: #21377]	Medium Name (multicellularity)	Suter with tyrosine

[Ref.: #21377]	Multicellular complex forming ability	no
[Ref.: #21377]	Medium Name (multicellularity)	Suter without tyrosine

Information on culture and growth conditions Culture and growth conditions

[Ref.: #17424]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #17424]

Culture medium growth

[Ref.: #17424]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17424]

Culture medium composition

expand / minimize

[Ref.: #21377]

Culture medium

ISP 2

[Ref.: #21377]

Culture medium growth

[Ref.: #21377]

Culture medium composition

expand / minimize

[Ref.: #21377]

Culture medium

ISP 3

[Ref.: #21377]

Culture medium growth

[Ref.: #21377]

Culture medium composition

expand / minimize

[Ref.: #21377]

Culture medium

ISP 4

[Ref.: #21377]

Culture medium growth

[Ref.: #21377]

Culture medium composition

expand / minimize

[Ref.: #21377]

Culture medium

ISP 5

[Ref.: #21377]

Culture medium growth

[Ref.: #21377]

Culture medium composition

expand / minimize

[Ref.: #21377]

Culture medium

ISP 7

[Ref.: #21377]

Culture medium growth

[Ref.: #21377]

Culture medium composition

expand / minimize

[Ref.: #21377]

Culture medium

Suter with tyrosine

[Ref.: #21377]

Culture medium growth

[Ref.: #21377]

Culture medium composition

expand / minimize

[Ref.: #21377]

Culture medium

Suter without tyrosine

[Ref.: #21377]

Culture medium growth

[Ref.: #21377]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #17424]	growth	28 °C
[Ref.: #29577]	growth	20-40 °C
[Ref.: #29577]	optimum	30 °C
[Ref.: #67770]	growth	28 °C
[Ref.: #67771]	growth	30 °C

[Ref.: #17424]	Temperature range	mesophilic
[Ref.: #29577]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29577]			optimum	7.5
[Ref.: #29577]			growth	06-11

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29577]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #29577]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.998

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #21377]		NaCl		maximum	10	%
[Ref.: #29577]		NaCl		growth	0-3	%

[Ref.: #67770]

Observation

quinones: MK-13, MK-14, MK-12

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #21377]	arabinose ChEBI	-
[Ref.: #29577]	arabinose ChEBI	+	carbon source
[Ref.: #21377]	cellulose ChEBI	+
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #29577]	esculin ChEBI	+	hydrolysis
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #21377]	fructose ChEBI	-
[Ref.: #68379]	gelatin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #21377]	glucose ChEBI	-
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #21377]	mannose ChEBI	-
[Ref.: #21377]	myo-inositol ChEBI	-
[Ref.: #21377]	raffinose ChEBI	-
[Ref.: #21377]	rhamnose ChEBI	-
[Ref.: #29577]	ribose ChEBI	+	carbon source
[Ref.: #21377]	sucrose ChEBI	-
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #21377]	xylose ChEBI	-
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym, API Coryne
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API Coryne
[Ref.: #29577]	catalase	+	1.11.1.6
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	+		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #68379]	urease	+	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #21377]	506	+/-	-	-	-	-	-	+	-	+	+	+	not determinedn.d.	+/-	-	-	+/-	+/-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #21377]	1018	not determinedn.d.	+/-	+/-	+	-	+	+	+/-	+	-	+	-	-	-	-	+	+/-	+/-	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17424]	Sample type/isolated from	cooling water
[Ref.: #17424]	Geographic location (country and/or sea, region)	Gwangyang
[Ref.: #17424]	Country	Republic of Korea
[Ref.: #17424]	Country ISO 3 Code	KOR
[Ref.: #17424]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Water-cooling system from an oxygen-producing plant
[Ref.: #67770]	Geographic location (country and/or sea, region)	Gwangyang
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From cooling water
[Ref.: #67771]	Geographic location (country and/or sea, region)	Gwangyang
[Ref.: #67771]	Country	Republic of Korea
[Ref.: #67771]	Country ISO 3 Code	KOR
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EF373534 (>99% sequence identity) for Chryseoglobus frigidaquae subclade from Microbeatlas

Aquatic Samples	417
Soil Samples	52
Animal Samples	139
Plant Samples	8
Total Samples	616

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17424]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #17424]	Chryseoglobus frigidaquae strain CW1 16S ribosomal RNA gene, partial sequence	EF373534	1420	ENA	424758 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Microcella frigidaquae DSM 23889	GCA_014200395	contig	GenBank	424758 tax ID	*
[Ref.: #66792]	Microcella frigidaquae DSM 23889	GCA_009664385	scaffold	GenBank	424758 tax ID	*
[Ref.: #66792]	Chryseoglobus frigidaquae strain DSM 23889	424758.4	wgs	PATRIC	424758 tax ID	*
[Ref.: #66792]	Chryseoglobus frigidaquae strain DSM 23889	424758.5	wgs	PATRIC	424758 tax ID	*
[Ref.: #66792]	Chryseoglobus frigidaquae DSM 23889	2845007952	draft	JGI IMG/M	424758 tax ID	*

[Ref.: #17424]	GC-content	68.3 mol%
[Ref.: #67770]	GC-content	68.3 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Chryseoglobus frigidaquae DSM 23889 NCBI: GCA_009664385.2
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Chryseoglobus frigidaquae DSM 23889 NCBI: GCA_009664385.1
[Ref.: #69480]	motile	no	87.84	yes
[Ref.: #69480]	flagellated	no	97.395	no
[Ref.: #69480]	gram-positive	yes	94.678	yes
[Ref.: #69480]	anaerobic	no	98.924	yes
[Ref.: #69480]	aerobic	yes	90.477	no
[Ref.: #69480]	halophile	no	92.913	no
[Ref.: #69480]	spore-forming	no	93.944	yes
[Ref.: #69480]	glucose-util	yes	84.015	no
[Ref.: #69480]	thermophile	no	98.011	yes
[Ref.: #69480]	glucose-ferment	no	87.777	no
[Ref.: #69480]	motile	no	87.84	yes
[Ref.: #69480]	flagellated	no	97.395	no
[Ref.: #69480]	gram-positive	yes	94.678	yes
[Ref.: #69480]	anaerobic	no	98.924	yes
[Ref.: #69480]	aerobic	yes	90.477	no
[Ref.: #69480]	halophile	no	92.913	no
[Ref.: #69480]	spore-forming	no	93.944	yes
[Ref.: #69480]	glucose-util	yes	84.015	no
[Ref.: #69480]	thermophile	no	98.011	yes
[Ref.: #69480]	glucose-ferment	no	87.777	no

Availability in culture collections External links

[Ref.: #17424]

Culture collection no.

DSM 23889, JCM 14730, KCTC 13142

[Ref.: #76952]

SI-ID 402936

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Microcella flavibacter sp. nov., isolated from marine sediment, and reclassification of Chryseoglobus frigidaquae, Chryseoglobus indicus, and Yonghaparkia alkaliphila as Microcella frigidaquae comb. nov., Microcella indica nom. nov., and Microcella alkalica nom. nov.	Xie F, Pei S, Huang X, Wang L, Kou J, Zhang G	Antonie Van Leeuwenhoek	10.1007/s10482-021-01668-7	2021	*
Phylogeny	Chryseoglobus indicus sp. nov., isolated from deep sea water.	Pei S, Xie F, Wang W, Zhang S, Zhang G	Int J Syst Evol Microbiol	10.1099/ijsem.0.004564	2020	*
Phylogeny	Chryseoglobus frigidaquae gen. nov., sp. nov., a novel member of the family Microbacteriaceae.	Baik KS, Park SC, Kim HJ, Lee KH, Seong CN	Int J Syst Evol Microbiol	10.1099/ijs.0.016212-0	2009	*

References

#17424

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23889

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21377

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#29577

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25970 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76952

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402936.1 )

#25970

IJSEM 1311 2010 ( DOI 10.1099/ijs.0.016212-0 , PubMed 19667371 )

* These data were automatically processed and therefore are not curated

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Chryseoglobus frigidaquae DSM 23889

Chryseoglobus frigidaquae DSM 23889

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