Glaciibacter superstes | Type strain | DSM 21135, NBRC 104264, AHU 1791

Caption:

electron microscopic image

Intellectual property rights:

Caption:

electron microscopic image

Intellectual property rights:

Caption:

electron microscopic image

Intellectual property rights:

Caption:

electron microscopic image

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

Culture medium growth

Medium recipe at MediaDive

Culture medium link

Culture medium composition

Culture medium

ISP 2

Culture medium growth

Culture medium composition

Culture medium

ISP 3

Culture medium growth

Culture medium composition

Culture medium

ISP 4

Culture medium growth

Culture medium composition

Culture medium

ISP 5

Culture medium growth

Culture medium composition

Culture medium

ISP 6

Culture medium growth

Culture medium composition

Culture medium

ISP 7

Culture medium growth

Culture medium composition

What are isolation sources categories?

		Temperature
[Ref.: #15488]	growth	15 °C
[Ref.: #18342]	optimum	28 °C
[Ref.: #28909]	optimum	20 °C

[Ref.: #15488]	Temperature range	psychrophilic
[Ref.: #18342]	Temperature range	mesophilic
[Ref.: #28909]	Temperature range	psychrophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #28909]

Oxygen tolerance

aerobe

[Ref.: #28909]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.78

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #28909]	arabinose ChEBI	+	carbon source
[Ref.: #28909]	cellobiose ChEBI	+	carbon source
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #28909]	fructose ChEBI	+	carbon source
[Ref.: #28909]	galactose ChEBI	+	carbon source
[Ref.: #68379]	gelatin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #28909]	glucose ChEBI	+	carbon source
[Ref.: #28909]	glycerol ChEBI	+	carbon source
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #28909]	maltose ChEBI	+	carbon source
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #28909]	mannitol ChEBI	+	carbon source
[Ref.: #28909]	mannose ChEBI	+	carbon source
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #28909]	rhamnose ChEBI	+	carbon source
[Ref.: #28909]	ribose ChEBI	+	carbon source
[Ref.: #28909]	sucrose ChEBI	+	carbon source
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #28909]	xylitol ChEBI	+	carbon source
[Ref.: #28909]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym, API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym, API Coryne
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym, API Coryne
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API Coryne
[Ref.: #28909]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	+		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym, API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68379]	pyrazinamidase	+	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	+	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #18342]	32	-	+	+	+	-	-	+	-	-	+	+	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18342]	52	not determinedn.d.	+	+	+	+	+	+	-	-	-	+	-	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15488]	Sample type/isolated from	ice wedge
[Ref.: #15488]	Geographic location (country and/or sea, region)	Alaska, Fox permafrost tunnel
[Ref.: #15488]	Country	USA
[Ref.: #15488]	Country ISO 3 Code	USA
[Ref.: #15488]	Continent	North America
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Ice

[Ref.: #69479]

Global distribution of 16S sequence AB378301 (>99% sequence identity) for Glaciibacter superstes subclade from Microbeatlas

Aquatic Samples	163
Soil Samples	217
Animal Samples	118
Plant Samples	109
Total Samples	607

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15488]	Glaciibacter superstes gene for 16S rRNA, partial sequence, strain: AHU1791	AB378301	1498	ENA	501023 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Glaciibacter superstes DSM 21135	GCA_000421145	scaffold	GenBank	1121924 tax ID	*
[Ref.: #66792]	Glaciibacter superstes DSM 21135	1121924.6	wgs	PATRIC	1121924 tax ID	*
[Ref.: #66792]	Glaciibacter superstes DSM 21135	2524614536	draft	JGI IMG/M	1121924 tax ID	*

GC-content

65 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Glaciibacter superstes DSM 21135 DSM 21135 NCBI: GCA_000421145.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Glaciibacter superstes DSM 21135 DSM 21135 NCBI: GCA_000421145.1
[Ref.: #69480]	motile	yes	63.55	no
[Ref.: #69480]	flagellated	no	93.884	no
[Ref.: #69480]	gram-positive	yes	90.174	yes
[Ref.: #69480]	anaerobic	no	99.453	yes
[Ref.: #69480]	aerobic	yes	93.737	yes
[Ref.: #69480]	halophile	no	91.585	no
[Ref.: #69480]	spore-forming	no	88.832	yes
[Ref.: #69480]	thermophile	no	99.015	no
[Ref.: #69480]	glucose-util	yes	86.71	yes
[Ref.: #69480]	glucose-ferment	no	88.162	yes

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