Name: Microcella putealis CV-2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7492

Type strain:

Species: Microcella putealis

Strain Designation: CV-2

Culture col. no.: DSM 19627, CIP 108471, LMG 22692, KCTC 19625

Strain history: CIP <- 2004, I. Tiago, Coimbra Univ., Coimbra, Portugal: strain CV-2

NCBI tax ID(s): 337005 (species)

SI-ID 132329

LMG 22692, CIP 108471, DSM 19627, KCTC 19625

Other strains from Microcella putealis

M. putealis CV-40, CIP 108474, LMG 22691
M. putealis AC-30, CIP 108472, LMG 22689

Section

Microcella putealis CV-2 is an obligate aerobe, mesophilic, rod-shaped bacterium that was isolated from alkaline groundwater.

rod-shaped
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Microbacteriaceae*
	Genus	*Microcella*
	Species	**Microcella putealis**
	Full Scientific Name (LPSN)	Microcella putealis Tiago et al. 2005

Information on morphological and physiological properties Morphology

[Ref.: #122067]	Cell shape	rod-shaped

[Ref.: #122067]	Motility	no

[Ref.: #122067]

Hemolysis ability

[Ref.: #122067]	Pigment production	no
[Ref.: #122067]	Pigment name	Flexirubin

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8219]

Culture medium

CASTENHOLZ MEDIUM (DSMZ Medium 86)

[Ref.: #8219]

Culture medium growth

[Ref.: #8219]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8219]

Culture medium composition

expand / minimize

[Ref.: #34321]

Culture medium

MEDIUM 591 - for No name

[Ref.: #34321]

Culture medium growth

[Ref.: #34321]

Culture medium composition

expand / minimize

[Ref.: #122067]

Culture medium

CIP Medium 591

[Ref.: #122067]

Culture medium growth

[Ref.: #122067]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #8219]		growth	35 °C
[Ref.: #34321]		growth	37 °C
[Ref.: #122067]		growth	25-37 °C
[Ref.: #122067]	no	growth	5 °C
[Ref.: #122067]	no	growth	15 °C
[Ref.: #122067]	no	growth	41 °C
[Ref.: #122067]	no	growth	45 °C

[Ref.: #8219]	Temperature range	mesophilic
[Ref.: #34321]	Temperature range	mesophilic
[Ref.: #122067]	Temperature range	mesophilic
[Ref.: #122067]	Temperature range	psychrophilic
[Ref.: #122067]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #122067]

Oxygen tolerance

obligate aerobe

	Salt	Tested relation		Salt conc.
[Ref.: #122067]	NaCl	no	growth	0	%
[Ref.: #122067]	NaCl	no	growth	2	%
[Ref.: #122067]	NaCl	no	growth	4	%
[Ref.: #122067]	NaCl	no	growth	6	%
[Ref.: #122067]	NaCl	no	growth	8	%
[Ref.: #122067]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #122067]	citrate ChEBI	-	carbon source
[Ref.: #122067]	esculin ChEBI	+	hydrolysis
[Ref.: #122067]	hippurate ChEBI	-	hydrolysis
[Ref.: #122067]	malonate ChEBI	-	assimilation
[Ref.: #122067]	nitrate ChEBI	-	builds gas from
[Ref.: #122067]	nitrate ChEBI	-	reduction
[Ref.: #122067]	nitrite ChEBI	-	builds gas from
[Ref.: #122067]	nitrite ChEBI	-	reduction

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #122067]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #122067]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #122067]		acetoin ChEBI	-
[Ref.: #122067]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #122067]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #122067]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #122067]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #122067]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #122067]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #122067]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #122067]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #122067]	oxidase	-
[Ref.: #122067]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #122067]	tryptophan deaminase	-
[Ref.: #122067]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #122067]	2762	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122067]	11209	-	-	+	+	-	+	+	-	-	-	-	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8219]	Sample type/isolated from	alkaline groundwater
[Ref.: #8219]	Geographic location (country and/or sea, region)	Cabeco de Vide
[Ref.: #8219]	Country	Portugal
[Ref.: #8219]	Country ISO 3 Code	PRT
[Ref.: #8219]	Continent	Europe

[Ref.: #122067]	Sample type/isolated from	Environment, Alkaline ground water
[Ref.: #122067]	Geographic location (country and/or sea, region)	Cabeco de Vide
[Ref.: #122067]	Country	Portugal
[Ref.: #122067]	Country ISO 3 Code	PRT
[Ref.: #122067]	Continent	Europe
[Ref.: #122067]	Isolation date	2000
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Groundwater
#Condition	#Alkaline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ717388 (>99% sequence identity) for Microcella from Microbeatlas

Aquatic Samples	882
Soil Samples	530
Animal Samples	298
Plant Samples	271
Total Samples	1981

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8219]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122067]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8219]	Microcella putealis 16S rRNA gene, type strain CV2T	AJ717388	1497	ENA	337005 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Microcella putealis DSM 19627	GCA_006716545	contig	GenBank	337005 tax ID	*
[Ref.: #66792]	Microcella putealis strain DSM 19627	337005.6	wgs	PATRIC	337005 tax ID	*
[Ref.: #66792]	Microcella putealis DSM 19627	2818991222	draft	JGI IMG/M	337005 tax ID	*

[Ref.: #8219]

GC-content

68.8 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Microcella putealis strain DSM 19627 PATRIC: 337005.6
[Ref.: #69480]	gram-positive	yes	93.674	no
[Ref.: #69480]	anaerobic	no	98.678	no
[Ref.: #69480]	halophile	no	73.325	no
[Ref.: #69480]	spore-forming	no	93.144	no
[Ref.: #69480]	glucose-util	yes	86.7	no
[Ref.: #69480]	motile	no	91.231	no
[Ref.: #69480]	flagellated	no	97.335	no
[Ref.: #69480]	thermophile	no	96.937	yes
[Ref.: #69480]	aerobic	yes	90.211	no
[Ref.: #69480]	glucose-ferment	no	87.431	no

Availability in culture collections External links

[Ref.: #8219]

Culture collection no.

DSM 19627, CIP 108471, LMG 22692, KCTC 19625

[Ref.: #76938]

SI-ID 132329

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Microcella flavibacter sp. nov., isolated from marine sediment, and reclassification of Chryseoglobus frigidaquae, Chryseoglobus indicus, and Yonghaparkia alkaliphila as Microcella frigidaquae comb. nov., Microcella indica nom. nov., and Microcella alkalica nom. nov.	Xie F, Pei S, Huang X, Wang L, Kou J, Zhang G	Antonie Van Leeuwenhoek	10.1007/s10482-021-01668-7	2021	*

References

#8219

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19627

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#34321 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76938

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID132329.1 )

#122067 Collection of Institut Pasteur ; Curators of the CIP; CIP 108471
* These data were automatically processed and therefore are not curated

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Microcella putealis strain DSM 19627

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