Subtercola boreus (DSM 13056, ATCC BAA-168, CCUG 43135)

Name: Subtercola boreus (DSM 13056, ATCC BAA-168, CCUG 43135)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7490

Type strain

Strain Designation K300

Culture col. no. DSM 13056 ATCC BAA-168 CCUG 43135 JCM 11267 K300 5 more

NCBI tax ID(s) 120213

Special Collections DSMZ CCUG JCM CIP Wink compendium

History <- M.K. Männistö; K300 2001, M. Männistö, Tampere Univ. Technol., Finland: strain K300 DSM 13056 <-- M. K. Männistö K300. CIP <- 2001, M. Männistö, Tampere Univ. Technol., Tampere, Finland: strain K300

Links

Subtercola boreus K300 is an aerobe, Gram-positive, rod-shaped bacterium that was isolated from groundwater.

Gram-positive rod-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 44310

ATCC BAA-168,DSM 13056,CCUG 43135,JCM 11267,NCIMB 13774,CIP 106947,IAM 15123,NBRC 103085,VKM Ac-2508

@ref 20215

Last LPSN update 2026-02-09 11:19:29

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Microbacteriaceae

Genus Subtercola

Species Subtercola boreus

Full scientific name Subtercola boreus Männistö et al. 2000

BacDive ID	Other strains from **Subtercola boreus** (1)	Type strain
103267	S. boreus ST033414(HKI),

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
116844	positive	rod-shaped
125438			90.5
125439	positive		98.4

Colony morphology

@ref	Colony color	Incubation period	Medium used
18504	Golden yellow (1004)	10-14 days	ISP 2
18504	Golden yellow (1004)	10-14 days	ISP 3
18504	Golden yellow (1004)	10-14 days	ISP 4
18504	Golden yellow (1004)	10-14 days	ISP 5
18504	Golden yellow (1004)	10-14 days	ISP 6
18504	Golden yellow (1004)	10-14 days	ISP 7
116844

Multicellular morphology

@ref	Forms multicellular complex	Medium name
18504		ISP 2
18504		ISP 3
18504		ISP 4
18504		ISP 5
18504		ISP 6
18504		ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
4889	R2A MEDIUM (DSMZ Medium 830)	Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
18504	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18504	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18504	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18504	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18504	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18504	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
40666	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
116844	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
4889	positive	growth	24
18504	positive	optimum	22
40666	positive	growth	20
55684	positive	growth	20
67770	positive	growth	17
116844	positive	growth	5-18

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
55684	aerobe
116844	obligate aerobe
125439	obligate aerobe	92.5

Halophily

@ref	Salt	Growth	Tested relation	Concentration
116844	NaCl	positive	growth	0-2 %
116844	NaCl		growth	4 %
116844	NaCl		growth	6 %
116844	NaCl		growth	8 %
116844	NaCl		growth	10 %

Murein

@ref	Murein short key	Type
4889	B07	B2gamma {Gly} [L-Dab] D-Glu-D-Dab

Observation

67770

Observationquinones: MK-10, MK-9, MK-11

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
18504	22599 ChEBI	arabinose	+
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
18504	62968 ChEBI	cellulose	+
116844	16947 ChEBI	citrate	-	carbon source
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	+	hydrolysis	from API Coryne
116844	4853 ChEBI	esculin	-	hydrolysis
18504	28757 ChEBI	fructose	+
68379	5291 ChEBI	gelatin	-	hydrolysis	from API Coryne
68368	5291 ChEBI	gelatin	-	hydrolysis	from API 20E
18504	17234 ChEBI	glucose	+
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
116844	606565 ChEBI	hippurate	+	hydrolysis
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68368	25094 ChEBI	lysine	-	degradation	from API 20E
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
18504	29864 ChEBI	mannitol	+
18504	17268 ChEBI	myo-inositol	+
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
116844	17632 ChEBI	nitrate	-	reduction
116844	17632 ChEBI	nitrate	-	respiration
116844	16301 ChEBI	nitrite	-	reduction
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
18504	16634 ChEBI	raffinose	+
18504	26546 ChEBI	rhamnose	+
18504	17992 ChEBI	sucrose	+
68379	17992 ChEBI	sucrose	-	fermentation	from API Coryne
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68379	16199 ChEBI	urea	-	hydrolysis	from API Coryne
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E
18504	18222 ChEBI	xylose	+

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
116844	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
116844	35581 ChEBI	indole
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test	Indole test
116844	15688 ChEBI	acetoin	-
68368	15688 ChEBI	acetoin	-			from API 20E
116844	17234 ChEBI	glucose		-
68368	35581 ChEBI	indole			-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
116844	alcohol dehydrogenase	-	1.1.1.1
68379	alkaline phosphatase	-	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
116844	amylase	-
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68382	beta-galactosidase	-	3.2.1.23	from API zym
116844	beta-galactosidase	+	3.2.1.23
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68382	beta-glucosidase	+	3.2.1.21	from API zym
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	+	3.2.1.31	from API Coryne
116844	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
116844	gamma-glutamyltransferase	-	2.3.2.2
116844	gelatinase	-
68379	gelatinase	-		from API Coryne
68368	gelatinase	-		from API 20E
116844	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
116844	lipase	-
68382	lipase (C 14)	-		from API zym
116844	lysine decarboxylase	-	4.1.1.18
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
116844	ornithine decarboxylase	-	4.1.1.17
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
116844	oxidase	-
116844	phenylalanine ammonia-lyase	-	4.3.1.24
68379	pyrazinamidase	+	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
68382	trypsin	-	3.4.21.4	from API zym
116844	tryptophan deaminase	-
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
116844	urease	-	3.5.1.5
68379	urease	-	3.5.1.5	from API Coryne
68368	urease	-	3.5.1.5	from API 20E

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
18504	+	-	-	-	+	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
18504	-	+	-	-	+	-	+	-	+	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
18504	not determinedn.d.	+	-	-	-	+	+	-	-	-	+	-	-	-	-	+	+	-	-	-
116844	-	-	+	+	-	+	-	-	-	-	+	+	-	-	-	+	+	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Groundwater

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
4889	groundwater		Finland	FIN	Europe
55684	Groundwater of a shallow aquifer (18m depth)	South,glacial gravel ridge	Finland	FIN	Europe
67770	Groundwater of a shallow aquifer under a glacial gravel ridge	Southern Finland	Finland	FIN	Europe
116844	Environment, Groundwater	Kärkölä	Finland	FIN	Europe	1996

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
4889	1	Risk group (German classification) According to TRBA 466
18504	1	German classification According to TRBA 466
116844	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM671611v1 assembly for Subtercola boreus DSM 13056	contig	GCA_006716115	120213.9	2814123445	120213	77.48
67770	ASM339968v1 assembly for Subtercola boreus K300	contig	GCA_003399685	120213.8	2832552210	120213	77.12

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Subtercola boreus partial 16S rRNA gene, type strain DSM 13056	AM410674	1467		120213
67770	Subtercola boreus 16S ribosomal RNA gene, partial sequence	AF224722	1467		120213

GC content

@ref	GC-content (mol%)	Method
4889	68	high performance liquid chromatography (HPLC)
67770	67.8	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Subtercola boreus K300
NCBI: GCA_003399685

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	92.50	no
125439	gram_stain	BacteriaNetⓘ	positive	98.40	no
125439	motility	BacteriaNetⓘ	no	86.60	no
125439	spore_formation	BacteriaNetⓘ	no	61.50	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Subtercola boreus strain DSM 13056
PATRIC: 120213.9

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	89.50	no
125438	anaerobic	anaerobicⓘ	no	96.14	no
125438	spore-forming	spore-formingⓘ	no	79.32	no
125438	aerobic	aerobicⓘ	yes	86.75	no
125438	thermophilic	thermophileⓘ	no	97.50	no
125438	flagellated	motile2+ⓘ	no	90.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from Abies koreana.	Jiang L, Peng Y, Seo J, Jeon D, Jo MG, Lee JH, Jeong JC, Kim CY, Park HC, Lee J.	Sci Rep	10.1038/s41598-022-16116-3	2022
Phylogeny	Subtercola vilae sp. nov., a novel actinobacterium from an extremely high-altitude cold volcano lake in Chile.	Villalobos AS, Wiese J, Aguilar P, Dorador C, Imhoff JF	Antonie Van Leeuwenhoek	10.1007/s10482-017-0994-4	2017
Phylogeny	Subtercola boreus gen. nov., sp. nov. and Subtercola frigoramans sp. nov., two new psychrophilic actinobacteria isolated from boreal groundwater.	Mannisto MK, Schumann P, Rainey FA, Kampfer P, Tsitko I, Tiirola MA, Salkinoja-Salonen MS	Int J Syst Evol Microbiol	10.1099/00207713-50-5-1731	2000

References

#4889	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13056
#18504	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#40666	; Curators of the CIP;
#55684	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 43135
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#116844	Collection of Institut Pasteur ; Curators of the CIP; CIP 106947
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Subtercola boreus (DSM 13056, ATCC BAA-168, CCUG 43135)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Murein

Observation

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API 20E

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_006716115

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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