Pseudoclavibacter helvolus DSM 20419 is an aerobe, Gram-positive, rod-shaped bacterium that was isolated from butter.
Gram-positive rod-shaped aerobe genome sequence 16S sequence Bacteria| @ref 20215 |
|
|
| Domain Bacteria |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Micrococcales |
| Family Microbacteriaceae |
| Genus Pseudoclavibacter |
| Species Pseudoclavibacter helvolus |
| Full scientific name Pseudoclavibacter helvolus Manaia et al. 2004 |
| Synonyms (1) |
| BacDive ID | Other strains from Pseudoclavibacter helvolus (1) | Type strain |
|---|---|---|
| 154433 | P. helvolus CCUG 53577 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 8802 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | Medium recipe at MediaDive | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water | ||
| 18745 | ISP 2 | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |||
| 18745 | ISP 3 | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |||
| 18745 | ISP 4 | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |||
| 18745 | ISP 5 | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |||
| 18745 | ISP 6 | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 18745 | ISP 7 | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 38283 | MEDIUM 72- for trypto casein soja agar | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |||
| 119870 | CIP Medium 111 | Medium recipe at CIP | |||
| 119870 | CIP Medium 72 | Medium recipe at CIP |
| @ref | Ability | Type | PH | PH range | |
|---|---|---|---|---|---|
| 30029 | positive | growth | 06-10 | alkaliphile |
| 30029 | Spore formationno |
| @ref | Murein short key | Type | |
|---|---|---|---|
| 8802 | B07 | B2gamma {Gly} [L-Dab] D-Glu-D-Dab |
| 67770 | Observationquinones: MK-9 |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 30029 | 62064 ChEBI | 2,3-butanediol | + | carbon source | |
| 30029 | 16449 ChEBI | alanine | + | carbon source | |
| 68371 | 27613 ChEBI | amygdalin | - | builds acid from | from API 50CH acid |
| 18745 | 22599 ChEBI | arabinose | + | ||
| 68371 | 18305 ChEBI | arbutin | - | builds acid from | from API 50CH acid |
| 18745 | 62968 ChEBI | cellulose | - | ||
| 119870 | 16947 ChEBI | citrate | - | carbon source | |
| 68371 | 17108 ChEBI | D-arabinose | - | builds acid from | from API 50CH acid |
| 68371 | 18333 ChEBI | D-arabitol | - | builds acid from | from API 50CH acid |
| 68371 | 28847 ChEBI | D-fucose | - | builds acid from | from API 50CH acid |
| 68371 | 12936 ChEBI | D-galactose | - | builds acid from | from API 50CH acid |
| 68371 | 62318 ChEBI | D-lyxose | - | builds acid from | from API 50CH acid |
| 68371 | 16988 ChEBI | D-ribose | - | builds acid from | from API 50CH acid |
| 68371 | 16443 ChEBI | D-tagatose | - | builds acid from | from API 50CH acid |
| 68371 | 65327 ChEBI | D-xylose | - | builds acid from | from API 50CH acid |
| 68371 | 17113 ChEBI | erythritol | - | builds acid from | from API 50CH acid |
| 30029 | 4853 ChEBI | esculin | + | hydrolysis | |
| 68379 | 4853 ChEBI | esculin | - | hydrolysis | from API Coryne |
| 119870 | 4853 ChEBI | esculin | - | hydrolysis | |
| 68371 | 4853 ChEBI | esculin | - | builds acid from | from API 50CH acid |
| 18745 | 28757 ChEBI | fructose | + | ||
| 30029 | 28757 ChEBI | fructose | + | carbon source | |
| 68371 | 16813 ChEBI | galactitol | - | builds acid from | from API 50CH acid |
| 68379 | 5291 ChEBI | gelatin | - | hydrolysis | from API Coryne |
| 68371 | 28066 ChEBI | gentiobiose | - | builds acid from | from API 50CH acid |
| 68371 | 24265 ChEBI | gluconate | - | builds acid from | from API 50CH acid |
| 18745 | 17234 ChEBI | glucose | + | ||
| 30029 | 17234 ChEBI | glucose | + | carbon source | |
| 30029 | 29987 ChEBI | glutamate | + | carbon source | |
| 30029 | 17754 ChEBI | glycerol | + | carbon source | |
| 68371 | 28087 ChEBI | glycogen | - | builds acid from | from API 50CH acid |
| 119870 | 606565 ChEBI | hippurate | + | hydrolysis | |
| 30029 | 17596 ChEBI | inosine | + | carbon source | |
| 68371 | 15443 ChEBI | inulin | - | builds acid from | from API 50CH acid |
| 68371 | 30849 ChEBI | L-arabinose | - | builds acid from | from API 50CH acid |
| 68371 | 18403 ChEBI | L-arabitol | - | builds acid from | from API 50CH acid |
| 68371 | 18287 ChEBI | L-fucose | - | builds acid from | from API 50CH acid |
| 68371 | 62345 ChEBI | L-rhamnose | - | builds acid from | from API 50CH acid |
| 68371 | 17266 ChEBI | L-sorbose | - | builds acid from | from API 50CH acid |
| 68371 | 65328 ChEBI | L-xylose | - | builds acid from | from API 50CH acid |
| 30029 | 17716 ChEBI | lactose | + | carbon source | |
| 30029 | 17306 ChEBI | maltose | + | carbon source | |
| 18745 | 29864 ChEBI | mannitol | + | ||
| 30029 | 29864 ChEBI | mannitol | + | carbon source | |
| 68371 | 6731 ChEBI | melezitose | - | builds acid from | from API 50CH acid |
| 68371 | 28053 ChEBI | melibiose | - | builds acid from | from API 50CH acid |
| 68371 | 320061 ChEBI | methyl alpha-D-glucopyranoside | - | builds acid from | from API 50CH acid |
| 68371 | 43943 ChEBI | methyl alpha-D-mannoside | - | builds acid from | from API 50CH acid |
| 68371 | 74863 ChEBI | methyl beta-D-xylopyranoside | - | builds acid from | from API 50CH acid |
| 30029 | 51850 ChEBI | methyl pyruvate | + | carbon source | |
| 18745 | 17268 ChEBI | myo-inositol | + | ||
| 30029 | 506227 ChEBI | N-acetylglucosamine | + | carbon source | |
| 119870 | 17632 ChEBI | nitrate | - | reduction | |
| 119870 | 17632 ChEBI | nitrate | - | respiration | |
| 119870 | 16301 ChEBI | nitrite | - | reduction | |
| 68371 | Potassium 2-ketogluconate | - | builds acid from | from API 50CH acid | |
| 30029 | 15361 ChEBI | pyruvate | + | carbon source | |
| 18745 | 16634 ChEBI | raffinose | - | ||
| 30029 | 16634 ChEBI | raffinose | + | carbon source | |
| 18745 | 26546 ChEBI | rhamnose | + | ||
| 68371 | 15963 ChEBI | ribitol | - | builds acid from | from API 50CH acid |
| 68371 | 17814 ChEBI | salicin | - | builds acid from | from API 50CH acid |
| 68371 | 28017 ChEBI | starch | - | builds acid from | from API 50CH acid |
| 18745 | 17992 ChEBI | sucrose | + | ||
| 30029 | 17992 ChEBI | sucrose | + | carbon source | |
| 68379 | 17992 ChEBI | sucrose | - | fermentation | from API Coryne |
| 30029 | 17748 ChEBI | thymidine | + | carbon source | |
| 68371 | 32528 ChEBI | turanose | - | builds acid from | from API 50CH acid |
| 30029 | 53426 ChEBI | tween 80 | + | carbon source | |
| 68379 | 16199 ChEBI | urea | - | hydrolysis | from API Coryne |
| 30029 | 16704 ChEBI | uridine | + | carbon source | |
| 68371 | 17151 ChEBI | xylitol | - | builds acid from | from API 50CH acid |
| 18745 | 18222 ChEBI | xylose | - |
| @ref | Metabolite | Is sensitive | Is resistant | |
|---|---|---|---|---|
| 119870 | 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate) |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 30029 | acid phosphatase | + | 3.1.3.2 | |
| 119870 | alcohol dehydrogenase | - | 1.1.1.1 | |
| 30029 | alkaline phosphatase | + | 3.1.3.1 | |
| 68382 | alkaline phosphatase | - | 3.1.3.1 | from API zym |
| 68379 | alkaline phosphatase | - | 3.1.3.1 | from API Coryne |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | - | 3.2.1.22 | from API zym |
| 68379 | alpha-glucosidase | + | 3.2.1.20 | from API Coryne |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 119870 | amylase | + | ||
| 68382 | beta-galactosidase | + | 3.2.1.23 | from API zym |
| 119870 | beta-galactosidase | + | 3.2.1.23 | |
| 68379 | beta-galactosidase | + | 3.2.1.23 | from API Coryne |
| 68382 | beta-glucosidase | - | 3.2.1.21 | from API zym |
| 68379 | beta-glucosidase | - | 3.2.1.21 | from API Coryne |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 68379 | beta-glucuronidase | - | 3.2.1.31 | from API Coryne |
| 119870 | caseinase | - | 3.4.21.50 | |
| 119870 | catalase | + | 1.11.1.6 | |
| 68382 | cystine arylamidase | - | 3.4.11.3 | from API zym |
| 30029 | cytochrome oxidase | + | 1.9.3.1 | |
| 119870 | DNase | + | ||
| 68382 | esterase (C 4) | + | from API zym | |
| 119870 | gamma-glutamyltransferase | - | 2.3.2.2 | |
| 119870 | gelatinase | +/- | ||
| 68379 | gelatinase | - | from API Coryne | |
| 119870 | lecithinase | + | ||
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 119870 | lipase | - | ||
| 68382 | lipase (C 14) | - | from API zym | |
| 119870 | lysine decarboxylase | - | 4.1.1.18 | |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API Coryne |
| 119870 | ornithine decarboxylase | - | 4.1.1.17 | |
| 119870 | oxidase | - | ||
| 119870 | phenylalanine ammonia-lyase | - | 4.3.1.24 | |
| 119870 | protease | - | ||
| 68379 | pyrazinamidase | - | 3.5.1.B15 | from API Coryne |
| 68379 | pyrrolidonyl arylamidase | + | 3.4.19.3 | from API Coryne |
| 68382 | trypsin | - | 3.4.21.4 | from API zym |
| 119870 | tryptophan deaminase | - | ||
| 119870 | tween esterase | + | ||
| 119870 | urease | - | 3.5.1.5 | |
| 68379 | urease | - | 3.5.1.5 | from API Coryne |
| @ref | ControlQ | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 119870 | not determinedn.d. | +/- | - | - | - | - | - | - | - | - | - | +/- | +/- | +/- | - | - | - | +/- | +/- | +/- | - | - | +/- | - | - | - | - | +/- | +/- | +/- | - | +/- | +/- | - | - | +/- | - | - | - | - | - | - | - | - | - | - | - | - | - | +/- |
Global distribution of 16S sequence X77440 (>99% sequence identity) for Pseudoclavibacter from Microbeatlas ![]()
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 66792 | ASM1419130v1 assembly for Pseudoclavibacter helvolus DSM 20419 | contig | 255205 | 59.34 | ||||
| 124043 | ASM4243138v1 assembly for Pseudoclavibacter helvolus JCM 9491 | scaffold | 255205 | 17.42 | ||||
| 124043 | ASM4265254v1 assembly for Pseudoclavibacter helvolus JCM 9491 | contig | 255205 | 6.57 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 8802 | Pseudoclavibacter helvolus 16S rRNA gene, type strain DSM 20419 | X77440 | 1462 | 255205 |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125439 | spore_formation | BacteriaNetⓘ | no | 77.40 | no |
| 125439 | motility | BacteriaNetⓘ | no | 81.40 | no |
| 125439 | gram_stain | BacteriaNetⓘ | positive | 99.10 | no |
| 125439 | oxygen_tolerance | BacteriaNetⓘ | obligate aerobe | 76.80 | no |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positiveⓘ | yes | 89.83 | yes |
| 125438 | anaerobic | anaerobicⓘ | no | 95.93 | yes |
| 125438 | spore-forming | spore-formingⓘ | no | 76.06 | no |
| 125438 | aerobic | aerobicⓘ | yes | 82.81 | yes |
| 125438 | thermophilic | thermophileⓘ | no | 95.91 | no |
| 125438 | flagellated | motile2+ⓘ | no | 89.50 | no |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Draft Genome Assembly of Two Pseudoclavibacter helvolus Strains, G8 and W3, Isolated from Slaughterhouse Environments. | Raghupathi PK, Herschend J, Roder HL, Sorensen SJ, Burmolle M. | Genome Announc | 10.1128/genomea.00077-16 | 2016 | ||
| Enzymology | Pseudoclavibacter otitis media in a 3-year-old boy with pulmonary and spinal tuberculosis. | Ayuthaya SIN, Leelaporn A, Kiratisin P, Oberdorfer P. | Medicine (Baltimore) | 10.1097/md.0000000000000709 | 2015 | |
| Phylogeny | Pseudoclavibacter endophyticus sp. nov., isolated from roots of Glycyrrhiza uralensis. | Li YQ, Li L, Fu YS, Cui ZQ, Duan YQ, Salam N, Guo JW, Chen W, Li WJ | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000876 | 2016 | |
| Phylogeny | Pseudoclavibacter chungangensis sp. nov., isolated from activated sludge. | Cho SL, Jung MY, Park MH, Chang YH, Yoon JH, Myung SC, Kim W | Int J Syst Evol Microbiol | 10.1099/ijs.0.015552-0 | 2009 | |
| Phylogeny | Pseudoclavibacter soli sp. nov., a {beta}-glucosidase-producing bacterium. | Kim MK, Jung HY | Int J Syst Evol Microbiol | 10.1099/ijs.0.65627-0 | 2009 | |
| Phylogeny | Zimmermannella helvola gen. nov., sp. nov., Zimmermannella alba sp. nov., Zimmermannella bifida sp. nov., Zimmermannella faecalis sp. nov. and Leucobacter albus sp. nov., novel members of the family Microbacteriaceae. | Lin YC, Uemori K, de Briel DA, Arunpairojana V, Yokota A | Int J Syst Evol Microbiol | 10.1099/ijs.0.02741-0 | 2004 | |
| Phylogeny | Gulosibacter molinativorax gen. nov., sp. nov., a molinate-degrading bacterium, and classification of 'Brevibacterium helvolum' DSM 20419 as Pseudoclavibacter helvolus gen. nov., sp. nov. | Manaia CM, Nogales B, Weiss N, Nunes OC | Int J Syst Evol Microbiol | 10.1099/ijs.0.02851-0 | 2004 |
| #8802 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20419 |
| #18745 | Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig . |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #26393 | IJSEM 783 2004 ( DOI 10.1099/ijs.0.02851-0 , PubMed 15143025 ) |
| #30029 | Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26393 |
| #38283 | ; Curators of the CIP; |
| #55779 | Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 43303 |
| #66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) . |
| #67770 | Japan Collection of Microorganism (JCM) ; Curators of the JCM; |
| #68371 | Automatically annotated from API 50CH acid . |
| #68379 | Automatically annotated from API Coryne . |
| #68382 | Automatically annotated from API zym . |
| #69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta . |
| #119870 | Collection of Institut Pasteur ; Curators of the CIP; CIP 108565 |
| #124043 | Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy . |
| #125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 ) |
| #125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 . |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
You found an error in BacDive? Please tell us about it!
Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.
Successfully sent
If you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive7475.20251217.10
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain data