Name: Mycetocola reblochoni BRB-1L41
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7471

Type strain:

Species: Mycetocola reblochoni

Strain Designation: BRB-1L41, R-20377

Culture col. no.: DSM 18580, JCM 30549, LMG 22367

Strain history: DSM 18580 <-- N. Bora and M. Goodfellow; Newcastle Univ., UK; R-20377 (=BRB-1L41).

NCBI tax ID(s): 331618 (species)

SI-ID 93763

LMG 22367, DSM 18580

Section

Mycetocola reblochoni BRB-1L41 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from surface of Reblochon cheese .

Gram-positive
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Microbacteriaceae*
	Genus	*Mycetocola*
	Species	**Mycetocola reblochoni**
	Full Scientific Name (LPSN)	Mycetocola reblochoni Bora et al. 2008

Information on morphological and physiological properties Morphology

[Ref.: #31759]	Gram stain	positive
[Ref.: #31759]	Cell length	1.1-1.5 µm
[Ref.: #31759]	Cell width	0.3 µm
[Ref.: #31759]	Cell shape	rod-shaped
[Ref.: #31759]	Motility	no

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69480]	Motility	no Confidence in %94.311

[Ref.: #18413]	Colony color	Zinc yellow (1018)
[Ref.: #18413]	Incubation period	10-14 days
[Ref.: #18413]	Cultivation medium used	ISP 2

[Ref.: #18413]	Colony color	Beige (1001)
[Ref.: #18413]	Incubation period	10-14 days
[Ref.: #18413]	Cultivation medium used	ISP 3

[Ref.: #18413]	Colony color	Beige (1001)
[Ref.: #18413]	Incubation period	10-14 days
[Ref.: #18413]	Cultivation medium used	ISP 4

[Ref.: #18413]	Colony color	Zinc yellow (1018)
[Ref.: #18413]	Incubation period	10-14 days
[Ref.: #18413]	Cultivation medium used	ISP 5

[Ref.: #18413]	Colony color	Zinc yellow (1018)
[Ref.: #18413]	Incubation period	10-14 days
[Ref.: #18413]	Cultivation medium used	ISP 6

[Ref.: #18413]	Colony color	Beige (1001)
[Ref.: #18413]	Incubation period	10-14 days
[Ref.: #18413]	Cultivation medium used	ISP 7

[Ref.: #31759]

Pigment production

yes

[Ref.: #18413]	Multicellular complex forming ability	no
[Ref.: #18413]	Medium Name (multicellularity)	ISP 2

[Ref.: #18413]	Multicellular complex forming ability	no
[Ref.: #18413]	Medium Name (multicellularity)	ISP 3

[Ref.: #18413]	Multicellular complex forming ability	no
[Ref.: #18413]	Medium Name (multicellularity)	ISP 4

[Ref.: #18413]	Multicellular complex forming ability	no
[Ref.: #18413]	Medium Name (multicellularity)	ISP 5

[Ref.: #18413]	Multicellular complex forming ability	no
[Ref.: #18413]	Medium Name (multicellularity)	ISP 6

[Ref.: #18413]	Multicellular complex forming ability	no
[Ref.: #18413]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7538]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #7538]

Culture medium growth

[Ref.: #7538]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7538]

Culture medium composition

expand / minimize

[Ref.: #18413]

Culture medium

ISP 2

[Ref.: #18413]

Culture medium growth

[Ref.: #18413]

Culture medium composition

expand / minimize

[Ref.: #18413]

Culture medium

ISP 3

[Ref.: #18413]

Culture medium growth

[Ref.: #18413]

Culture medium composition

expand / minimize

[Ref.: #18413]

Culture medium

ISP 4

[Ref.: #18413]

Culture medium growth

[Ref.: #18413]

Culture medium composition

expand / minimize

[Ref.: #18413]

Culture medium

ISP 5

[Ref.: #18413]

Culture medium growth

[Ref.: #18413]

Culture medium composition

expand / minimize

[Ref.: #18413]

Culture medium

ISP 6

[Ref.: #18413]

Culture medium growth

[Ref.: #18413]

Culture medium composition

expand / minimize

[Ref.: #18413]

Culture medium

ISP 7

[Ref.: #18413]

Culture medium growth

[Ref.: #18413]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #7538]	growth	28 °C
[Ref.: #18413]	optimum	28 °C
[Ref.: #31759]	growth	20-30 °C
[Ref.: #31759]	optimum	20-30 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #7538]	Temperature range	mesophilic
[Ref.: #18413]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31759]			optimum	07-10
[Ref.: #31759]			growth	07-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31759]

Oxygen tolerance

aerobe

[Ref.: #31759]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.999

[Ref.: #31759]	Observation	aggregates in chains
[Ref.: #67770]	Observation	quinones: MK-10

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #31759]	mannose ChEBI	+	carbon source
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #31759]	salicin ChEBI	+	carbon source
[Ref.: #31759]	sucrose ChEBI	+	carbon source
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #31759]	trehalose ChEBI	+	carbon source
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym, API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	+	3.2.1.23	* from API Coryne
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym, API Coryne
[Ref.: #68382]	beta-glucuronidase	+	3.2.1.31	* from API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne
[Ref.: #31759]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym, API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68379]	pyrazinamidase	+	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #18413]	95	-	+	-	-	-	+	+	-	+	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18413]	123	not determinedn.d.	-	+	+	-	+	+	-	-	-	+	-	-	-	+	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7538]	Sample type/isolated from	surface of Reblochon cheese (late stage of ripening)

[Ref.: #67770]	Sample type/isolated from	Surface of Reblochon cheese at the late stage of ripening
* marker position based on {}

Isolation sources categories

#Engineered

#Food production

#Dairy product

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7538]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7538]	Mycetocola reblochoni strain LMG 22367 16S ribosomal RNA gene, partial sequence	DQ062097	1400	ENA	331618 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Mycetocola reblochoni JCM 30549	GCA_003667525	contig	GenBank	331618 tax ID
[Ref.: #66792]	Mycetocola reblochoni strain JCM 30549	331618.3	wgs	PATRIC	331618 tax ID	*
[Ref.: #66792]	Mycetocola reblochoni JCM 30549	2866450942	draft	JGI IMG/M	331618 tax ID	*

[Ref.: #7538]	GC-content	70.0 mol%
[Ref.: #31759]	GC-content	70 mol%
[Ref.: #67770]	GC-content	70 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Mycetocola reblochoni strain JCM 30549 PATRIC: 331618.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Mycetocola reblochoni strain JCM 30549 PATRIC: 331618.3
[Ref.: #69480]	gram-positive	yes	94.779	no
[Ref.: #69480]	anaerobic	no	99.386	no
[Ref.: #69480]	halophile	no	61.558	no
[Ref.: #69480]	spore-forming	no	94.29	no
[Ref.: #69480]	glucose-util	yes	87.618	no
[Ref.: #69480]	motile	no	95.652	no
[Ref.: #69480]	flagellated	no	97.018	yes
[Ref.: #69480]	thermophile	no	99.63	yes
[Ref.: #69480]	aerobic	yes	91.057	no
[Ref.: #69480]	glucose-ferment	no	87.406	yes

Availability in culture collections External links

[Ref.: #7538]

Culture collection no.

DSM 18580, JCM 30549, LMG 22367

[Ref.: #76916]

SI-ID 93763

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Mycetocola miduiensis sp. nov., a psychrotolerant bacterium isolated from Midui glacier.	Zhu L, Liu Q, Liu H, Zhou Y, Xin Y, Dong X	Int J Syst Evol Microbiol	10.1099/ijs.0.047985-0	2013	*
Phylogeny	Mycetocola reblochoni sp. nov., isolated from the surface microbial flora of Reblochon cheese.	Bora N, Vancanneyt M, Gelsomino R, Snauwaert C, Swings J, Jones AL, Ward AC, Chamba JF, Kroppenstedt RM, Schumann P, Goodfellow M	Int J Syst Evol Microbiol	10.1099/ijs.0.64201-0	2008	*

References

#7538

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18580

#18413

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31759

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28032 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76916

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID93763.1 )

#28032

IJSEM 2687 2008 ( DOI 10.1099/ijs.0.64201-0 , PubMed 19060041 )

* These data were automatically processed and therefore are not curated

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Mycetocola reblochoni strain JCM 30549

Mycetocola reblochoni strain JCM 30549

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