Name: Microbacterium lindanitolerans MNA2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7453

Type strain:

Species: Microbacterium lindanitolerans

Strain Designation: MNA2

Culture col. no.: DSM 22422, CCM 7585, JCM 30493

Strain history: CCM 7585 <-- R. Lal; Univ. of Delhi, India; MNA2.

NCBI tax ID(s): 550448 (species)

SI-ID 371118

DSM22422, CCM 7585

Section

Microbacterium lindanitolerans MNA2 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from hexachlorocyclohexane contaminated soil.

Gram-positive
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Microbacteriaceae*
	Genus	*Microbacterium*
	Species	**Microbacterium lindanitolerans**
	Full Scientific Name (LPSN)	Microbacterium lindanitolerans Lal et al. 2010

Information on morphological and physiological properties Morphology

[Ref.: #29641]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #29641]	Cell shape	rod-shaped

[Ref.: #29641]	Motility	no

[Ref.: #21414]	Colony color	Rape yellow (1021)
[Ref.: #21414]	Incubation period	10-14 days
[Ref.: #21414]	Cultivation medium used	ISP 2

[Ref.: #21414]	Colony color	Zinc yellow (1018)
[Ref.: #21414]	Incubation period	10-14 days
[Ref.: #21414]	Cultivation medium used	ISP 3

[Ref.: #21414]	Colony color	Rape yellow (1021)
[Ref.: #21414]	Incubation period	10-14 days
[Ref.: #21414]	Cultivation medium used	ISP 4

[Ref.: #21414]	Colony color	Zinc yellow (1018)
[Ref.: #21414]	Incubation period	10-14 days
[Ref.: #21414]	Cultivation medium used	ISP 5

[Ref.: #21414]	Colony color	Zinc yellow (1018)
[Ref.: #21414]	Incubation period	10-14 days
[Ref.: #21414]	Cultivation medium used	ISP 7

[Ref.: #21414]	Colony color	Ziny yellow (1018)
[Ref.: #21414]	Incubation period	10-14 days
[Ref.: #21414]	Cultivation medium used	Suter with tyrosine

[Ref.: #21414]	Colony color	Zinc yellow (1018)
[Ref.: #21414]	Incubation period	10-14 days
[Ref.: #21414]	Cultivation medium used	Suter without tyrosine

[Ref.: #29641]

Pigment production

yes

[Ref.: #21414]	Multicellular complex forming ability	no
[Ref.: #21414]	Medium Name (multicellularity)	ISP 2

[Ref.: #21414]	Multicellular complex forming ability	no
[Ref.: #21414]	Medium Name (multicellularity)	ISP 3

[Ref.: #21414]	Multicellular complex forming ability	no
[Ref.: #21414]	Medium Name (multicellularity)	ISP 4

[Ref.: #21414]	Multicellular complex forming ability	no
[Ref.: #21414]	Medium Name (multicellularity)	ISP 5

[Ref.: #21414]	Multicellular complex forming ability	no
[Ref.: #21414]	Medium Name (multicellularity)	ISP 7

[Ref.: #21414]	Multicellular complex forming ability	no
[Ref.: #21414]	Medium Name (multicellularity)	Suter with tyrosine

[Ref.: #21414]	Multicellular complex forming ability	no
[Ref.: #21414]	Medium Name (multicellularity)	Suter without tyrosine

Information on culture and growth conditions Culture and growth conditions

[Ref.: #21414]

Culture medium

ISP 2

[Ref.: #21414]

Culture medium growth

[Ref.: #21414]

Culture medium composition

expand / minimize

[Ref.: #21414]

Culture medium

ISP 3

[Ref.: #21414]

Culture medium growth

[Ref.: #21414]

Culture medium composition

expand / minimize

[Ref.: #21414]

Culture medium

ISP 4

[Ref.: #21414]

Culture medium growth

[Ref.: #21414]

Culture medium composition

expand / minimize

[Ref.: #21414]

Culture medium

ISP 5

[Ref.: #21414]

Culture medium growth

[Ref.: #21414]

Culture medium composition

expand / minimize

[Ref.: #21414]

Culture medium

ISP 7

[Ref.: #21414]

Culture medium growth

[Ref.: #21414]

Culture medium composition

expand / minimize

[Ref.: #21414]

Culture medium

Suter with tyrosine

[Ref.: #21414]

Culture medium growth

[Ref.: #21414]

Culture medium composition

expand / minimize

[Ref.: #21414]

Culture medium

Suter without tyrosine

[Ref.: #21414]

Culture medium growth

[Ref.: #21414]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #29641]	growth	25-37 °C
[Ref.: #29641]	optimum	28-37 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #29641]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29641]			optimum	07-08
[Ref.: #29641]			growth	06-11

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29641]

Oxygen tolerance

aerobe

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.994

	Salt		Salt conc.
[Ref.: #21414]	NaCl	maximum	10	%
[Ref.: #29641]	NaCl	growth	0-2	%
[Ref.: #29641]	NaCl	optimum	0-2	%

[Ref.: #29641]	Observation	aggregates in chains
[Ref.: #67770]	Observation	quinones: MK-12, MK-11, MK-13

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #21414]	arabinose ChEBI	+
[Ref.: #29641]	cellobiose ChEBI	+	carbon source
[Ref.: #21414]	cellulose ChEBI	-
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #29641]	esculin ChEBI	+	hydrolysis
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #21414]	fructose ChEBI	-
[Ref.: #29641]	fructose ChEBI	+	carbon source
[Ref.: #29641]	galactose ChEBI	+	carbon source
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #21414]	glucose ChEBI	+
[Ref.: #29641]	glucose ChEBI	+	carbon source
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #29641]	lactose ChEBI	+	carbon source
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #21414]	mannose ChEBI	+
[Ref.: #21414]	myo-inositol ChEBI	-
[Ref.: #29641]	nitrate ChEBI	+	reduction
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #21414]	raffinose ChEBI	-
[Ref.: #21414]	rhamnose ChEBI	+
[Ref.: #29641]	ribose ChEBI	+	carbon source
[Ref.: #29641]	sorbitol ChEBI	+	carbon source
[Ref.: #21414]	sucrose ChEBI	+
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #21414]	xylose ChEBI	+
[Ref.: #29641]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym, API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym, API Coryne
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne
[Ref.: #29641]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	+	3.5.1.5	* from API Coryne
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #21414]	531	-	-	-	-	-	+	+	-	-	+	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #21414]	1051	not determinedn.d.	-	+/-	+/-	-	+	+/-	-	-	-	+/-	+/-	-	+	+/-	+	+	+	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16328]	Sample type/isolated from	hexachlorocyclohexane contaminated soil
[Ref.: #16328]	Country	India
[Ref.: #16328]	Country ISO 3 Code	IND
[Ref.: #16328]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Hexachlorocyclohexane-contaminated soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	North India
[Ref.: #67770]	Country	India
[Ref.: #67770]	Country ISO 3 Code	IND
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Contamination	-
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EU873539 (>99% sequence identity) for Microbacterium from Microbeatlas

Aquatic Samples	24
Soil Samples	138
Animal Samples	30
Plant Samples	32
Total Samples	224

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16328]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #21414]	Biosafety level	L1	German classification

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16328]	Microbacterium lindanitolerans strain MNA2 16S ribosomal RNA gene, partial sequence	EU873539	1289	ENA	550448 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Microbacterium lindanitolerans DSM 22422	GCA_006788875	contig	GenBank	550448 tax ID
[Ref.: #66792]	Microbacterium lindanitolerans strain DSM 22422	550448.3	wgs	PATRIC	550448 tax ID	*
[Ref.: #66792]	Microbacterium lindanitolerans DSM 22422	2811994985	draft	JGI IMG/M	550448 tax ID	*

[Ref.: #16328]	GC-content	65.3 mol%	high performance liquid chromatography (HPLC)
[Ref.: #29641]	GC-content	65.3 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Microbacterium lindanitolerans strain DSM 22422 PATRIC: 550448.3
[Ref.: #69481]	spore-forming	no	88	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Microbacterium lindanitolerans strain DSM 22422 PATRIC: 550448.3
[Ref.: #69480]	gram-positive	yes	91.118	yes
[Ref.: #69480]	anaerobic	no	99.504	yes
[Ref.: #69480]	halophile	no	65.558	no
[Ref.: #69480]	spore-forming	no	92.204	no
[Ref.: #69480]	glucose-util	yes	89.117	yes
[Ref.: #69480]	thermophile	no	96.883	yes
[Ref.: #69480]	aerobic	yes	92.302	yes
[Ref.: #69480]	motile	yes	55.205	yes
[Ref.: #69480]	flagellated	no	93.85	yes
[Ref.: #69480]	glucose-ferment	no	86.799	no

Availability in culture collections External links

[Ref.: #16328]

Culture collection no.

DSM 22422, CCM 7585, JCM 30493

[Ref.: #76900]

SI-ID 371118

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Microbacterium chengjingii sp. nov. and Microbacterium fandaimingii sp. nov., isolated from bat faeces of Hipposideros and Rousettus species.	Zhou J, Huang Y, Yang J, Lai XH, Jin D, Lu S, Pu J, Zhang S, Zhu W, Xu M, Huang Y, Liang J, Zheng H, Xu J	Int J Syst Evol Microbiol	10.1099/ijsem.0.004858	2021	*
Phylogeny	Microbacterium lindanitolerans sp. nov., isolated from hexachlorocyclohexane-contaminated soil.	Lal D, Gupta SK, Schumann P, Lal R	Int J Syst Evol Microbiol	10.1099/ijs.0.017699-0	2009	*

References

#16328

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22422

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21414

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#29641

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26030 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76900

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID371118.1 )

#26030

IJSEM 2634 2010 ( DOI 10.1099/ijs.0.017699-0 , PubMed 20023059 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Microbacterium lindanitolerans in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Microbacterium lindanitolerans strain DSM 22422

Microbacterium lindanitolerans strain DSM 22422

Change proposal

Change proposal