Microbacterium insulae (DSM 23024, CCUG 54523, KCTC 19247)

Name: Microbacterium insulae (DSM 23024, CCUG 54523, KCTC 19247)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7447

Type strain

Strain Designation DS-66

Culture col. no. DSM 23024 CCUG 54523 KCTC 19247 JCM 19115 DS-66

NCBI tax ID(s) 483014

Special Collections DSMZ CCUG JCM KCTC Literature strains

Links

Microbacterium insulae DS-66 is an aerobe, mesophilic, Gram-positive prokaryote that was isolated from soil.

Gram-positive coccus-shaped aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 407518

KCTC 19247,JCM 19115,DSM 23024,CCUG 54523

@ref 20215

Last LPSN update 2025-12-16 09:48:35

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Microbacteriaceae

Genus Microbacterium

Species Microbacterium insulae

Full scientific name Microbacterium insulae Yoon et al. 2009

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape
29157	positive	1.15 µm	0.35 µm	coccus-shaped
67771	positive
125438
125438	positive
125439	positive

Colony morphology

60213

Incubation period1-2 days

Pigmentation

29157

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
16654	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)		Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
16654	positive	growth	28	mesophilic
29157	positive	growth	20-38
29157	positive	optimum	30	mesophilic
60213	positive	growth	30-37	mesophilic
67770	positive	growth	28	mesophilic
67771	positive	growth	30	mesophilic

Culture pH

@ref	Ability	Type	PH
29157	positive	optimum	6.75
29157	positive	growth	5.5-8.0

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
60213	aerobe
67771	aerobe
125439	obligate aerobe

Spore formation

29157

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
29157	NaCl	positive	growth	0-5 %
29157	NaCl	positive	optimum	0.75 %

Murein

@ref	Murein short key	Type
16654	B06	B2ß {Gly} [L-Hsr] D-Glu(Hyg)-Gly-D-Orn

Observation

67770

Observationquinones: MK-13, MK-12, MK-14

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
29157	22599 ChEBI	arabinose	+	carbon source
29157	17057 ChEBI	cellobiose	+	carbon source
29157	4853 ChEBI	esculin	+	hydrolysis
29157	28757 ChEBI	fructose	+	carbon source
29157	28260 ChEBI	galactose	+	carbon source
29157	5291 ChEBI	gelatin	+	carbon source
29157	17234 ChEBI	glucose	+	carbon source
29157	17754 ChEBI	glycerol	+	carbon source
29157	17716 ChEBI	lactose	+	carbon source
29157	17306 ChEBI	maltose	+	carbon source
29157	29864 ChEBI	mannitol	+	carbon source
29157	37684 ChEBI	mannose	+	carbon source
29157	37657 ChEBI	methyl D-glucoside	+	carbon source
29157	26546 ChEBI	rhamnose	+	carbon source
29157	17814 ChEBI	salicin	+	carbon source
29157	17992 ChEBI	sucrose	+	carbon source
29157	27082 ChEBI	trehalose	+	carbon source
29157	53424 ChEBI	tween 20	+	carbon source
29157	53423 ChEBI	tween 40	+	carbon source
29157	53425 ChEBI	tween 60	+	carbon source
29157	53426 ChEBI	tween 80	+	carbon source
29157	18222 ChEBI	xylose	+	carbon source

Enzymes

@ref	Value	Activity	Ec
29157	cytochrome oxidase	+	1.9.3.1

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
16654	soil	Dokdo	Republic of Korea	KOR	Asia
60213	Soil	Dokdo	Republic of Korea	KOR	Asia
67770	Soil
67771	From soil	Dokdo	Republic of Korea	KOR	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
16654	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	IMG accession	NCBI tax ID	Score
66792	Microbacterium insulae DSM 23024	complete	2893599951	483014	99.79
66792	Microbacterium insulae strain DSM 23024	contig		483014	79.8
124043	ASM4267706v1 assembly for Microbacterium insulae CCUG 54523	contig		483014	77.28

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
16654	Microbacterium insulae strain DS-66 16S ribosomal RNA gene, partial sequence	EU239498	1447		483014

GC content

@ref	GC-content (mol%)	Method
16654	69.9	high performance liquid chromatography (HPLC)
29157	69.9

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Microbacterium insulae DSM 23024
IMG: 2893599951

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	76.50	no
125439	motility	BacteriaNetⓘ	no	71.40	no
125439	gram_stain	BacteriaNetⓘ	positive	99.30	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.50	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Microbacterium insulae strain DSM 23024
PATRIC: 483014.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	91.34	no
125438	anaerobic	anaerobicⓘ	no	97.67	yes
125438	spore-forming	spore-formingⓘ	no	72.37	yes
125438	aerobic	aerobicⓘ	yes	89.26	yes
125438	thermophilic	thermophileⓘ	no	96.43	yes
125438	flagellated	motile2+ⓘ	no	93.00	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Microbacterium aureliae sp. nov., a novel actinobacterium isolated from Aurelia aurita, the moon jellyfish.	Kaur G, Mual P, Kumar N, Verma A, Kumar A, Krishnamurthi S, Mayilraj S	Int J Syst Evol Microbiol	10.1099/ijsem.0.001407	2016
Phylogeny	Microbacterium marinum sp. nov., isolated from deep-sea water.	Zhang L, Xi L, Ruan J, Huang Y	Syst Appl Microbiol	10.1016/j.syapm.2011.11.004	2012
Phylogeny	Microbacterium arthrosphaerae sp. nov., isolated from the faeces of the pill millipede Arthrosphaera magna Attems.	Kampfer P, Rekha PD, Schumann P, Arun AB, Young CC, Chen WM, Sridhar KR	Int J Syst Evol Microbiol	10.1099/ijs.0.026401-0	2010
Phylogeny	Microbacterium insulae sp. nov., isolated from soil.	Yoon JH, Schumann P, Kang SJ, Lee CS, Lee SY, Oh TK	Int J Syst Evol Microbiol	10.1099/ijs.0.007591-0	2009

References

#16654	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23024
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#29157	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25581 (see below)
#60213	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 54523
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Microbacterium insulae (DSM 23024, CCUG 54523, KCTC 19247)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Murein

Observation

Metabolite utilization

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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