Name: Microbacterium kribbense DSM 19265
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7442

Type strain:

Species: Microbacterium kribbense

Culture col. no.: DSM 19265, KCTC 19269, MSL 04, JCM 16950

Strain history: <- CJ Kim, KRIBB

NCBI tax ID(s): 433645 (species)

SI-ID 398019

DSM 19265

Section

Microbacterium kribbense DSM 19265 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from soil.

Gram-positive
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Microbacteriaceae*
	Genus	*Microbacterium*
	Species	**Microbacterium kribbense**
	Full Scientific Name (LPSN)	Microbacterium kribbense Dastager et al. 2008

Information on morphological and physiological properties Morphology

[Ref.: #31204]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive

[Ref.: #31204]	Cell length	0.5-0.8 µm

[Ref.: #31204]	Cell width	0.4-0.6 µm

[Ref.: #31204]	Cell shape	rod-shaped

[Ref.: #31204]	Motility	no

[Ref.: #18332]	Colony color	Colourless
[Ref.: #18332]	Incubation period	10-14 days
[Ref.: #18332]	Cultivation medium used	ISP 2

[Ref.: #18332]	Incubation period	10-14 days
[Ref.: #18332]	Cultivation medium used	ISP 3

[Ref.: #18332]	Incubation period	10-14 days
[Ref.: #18332]	Cultivation medium used	ISP 7

[Ref.: #31204]

Pigment production

yes

[Ref.: #18332]	Multicellular complex forming ability	no
[Ref.: #18332]	Medium Name (multicellularity)	ISP 2

[Ref.: #18332]	Multicellular complex forming ability	no
[Ref.: #18332]	Medium Name (multicellularity)	ISP 3

[Ref.: #18332]	Multicellular complex forming ability	no
[Ref.: #18332]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7914]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #7914]

Culture medium growth

[Ref.: #7914]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7914]

Culture medium composition

expand / minimize

[Ref.: #18332]

Culture medium

ISP 2

[Ref.: #18332]

Culture medium growth

[Ref.: #18332]

Culture medium composition

expand / minimize

[Ref.: #18332]

Culture medium

ISP 3

[Ref.: #18332]

Culture medium growth

[Ref.: #18332]

Culture medium composition

expand / minimize

[Ref.: #18332]

Culture medium

ISP 7

[Ref.: #18332]

Culture medium growth

[Ref.: #18332]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #7914]	growth	28 °C
[Ref.: #18332]	optimum	28 °C
[Ref.: #31204]	growth	20-37 °C
[Ref.: #31204]	optimum	28 °C
[Ref.: #67770]	growth	28 °C
[Ref.: #67771]	growth	30 °C

[Ref.: #7914]	Temperature range	mesophilic
[Ref.: #18332]	Temperature range	mesophilic
[Ref.: #31204]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31204]			optimum	7.2-7.4
[Ref.: #31204]			growth	07-11

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31204]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31204]		NaCl		growth	0-3	%
[Ref.: #31204]		NaCl		optimum	0-3	%

[Ref.: #7914]	Murein short key	B06
[Ref.: #7914]	Murein types	B2ß {Gly} [L-Hsr] D-Glu(Hyg)-Gly-D-Orn

[Ref.: #31204]	Observation	aggregates in chains
[Ref.: #67770]	Observation	quinones: MK-11, MK-12

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31204]	arabinose ChEBI	+	carbon source
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #31204]	esculin ChEBI	+	hydrolysis
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #31204]	galactose ChEBI	+	carbon source
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #31204]	glucose ChEBI	+	carbon source
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #31204]	maltose ChEBI	+	carbon source
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #31204]	mannitol ChEBI	+	carbon source
[Ref.: #31204]	mannose ChEBI	+	carbon source
[Ref.: #31204]	melibiose ChEBI	+	carbon source
[Ref.: #31204]	nitrate ChEBI	+	reduction
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #31204]	raffinose ChEBI	+	carbon source
[Ref.: #31204]	rhamnose ChEBI	+	carbon source
[Ref.: #31204]	sucrose ChEBI	+	carbon source
[Ref.: #68379]	sucrose ChEBI	+	fermentation	* from API Coryne
[Ref.: #31204]	trehalose ChEBI	+	carbon source
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #31204]		hydrogen sulfide ChEBI	yes

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym, API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym, API Coryne
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym, API Coryne
[Ref.: #68382]	beta-glucuronidase	+	3.2.1.31	* from API zym, API Coryne
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym, API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #18332]	25	-	-	-	-	+	-	+	+	+	-	-	not determinedn.d.	-	-	-	-	-	-	+	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18332]	42	not determinedn.d.	-	+	+	-	+	+	-	+	-	+	-	+	-	+	+	+	+	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7914]	Sample type/isolated from	soil
[Ref.: #7914]	Geographic location (country and/or sea, region)	Bigeum Island
[Ref.: #7914]	Country	Republic of Korea
[Ref.: #7914]	Country ISO 3 Code	KOR
[Ref.: #7914]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Soil from Bigeum Island
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From farming field
[Ref.: #67771]	Geographic location (country and/or sea, region)	Bikeum island
[Ref.: #67771]	Country	Republic of Korea
[Ref.: #67771]	Country ISO 3 Code	KOR
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7914]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7914]	Microbacterium kribbense strain MSL 04 16S ribosomal RNA gene, partial sequence	EF466125	1428	ENA	433645 tax ID

[Ref.: #7914]	GC-content	71.0 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #31204]	GC-content	71 mol%
[Ref.: #67770]	GC-content	71 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #7914]

Culture collection no.

DSM 19265, KCTC 19269, MSL 04, JCM 16950

[Ref.: #76890]

SI-ID 398019

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Microbacterium kribbense sp. nov., isolated from soil.	Dastager SG, Lee JC, Ju YJ, Park DJ, Kim CJ	Int J Syst Evol Microbiol	10.1099/ijs.0.2008/001222-0	2008	*

References

#7914

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19265

#18332

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31204

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27531 (see below)

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#76890

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID398019.1 )

#27531

IJSEM 2536 2008 ( DOI 10.1099/ijs.0.2008/001222-0 , PubMed 18984689 )

* These data were automatically processed and therefore are not curated

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