Name: Microbacterium natoriense TNJL143-2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7425

Type strain:

Species: Microbacterium natoriense

Strain Designation: TNJL143-2

Culture col. no.: DSM 17277, ATCC BAA 1032, CIP 108753, JCM 12611

Strain history: T. Nakayama TNJL143-2.

NCBI tax ID(s): 284570 (species)

Section

Microbacterium natoriense TNJL143-2 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from soil.

Gram-positive
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Microbacteriaceae*
	Genus	*Microbacterium*
	Species	**Microbacterium natoriense**
	Full Scientific Name (LPSN)	Microbacterium natoriense Liu et al. 2005

Information on morphological and physiological properties Morphology

[Ref.: #31304]	Gram stain	positive
[Ref.: #31304]	Cell length	1.5 µm
[Ref.: #31304]	Cell width	0.55 µm
[Ref.: #31304]	Cell shape	rod-shaped
[Ref.: #31304]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6869]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #6869]

Culture medium growth

[Ref.: #6869]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6869]

Culture medium composition

expand / minimize

[Ref.: #6869]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #6869]

Culture medium growth

[Ref.: #6869]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6869]

Culture medium composition

expand / minimize

[Ref.: #37641]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37641]

Culture medium growth

[Ref.: #37641]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #6869]	growth	28 °C
[Ref.: #31304]	growth	10-50 °C
[Ref.: #31304]	optimum	30 °C
[Ref.: #37641]	growth	30 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #6869]	Temperature range	mesophilic
[Ref.: #31304]	Temperature range	mesophilic
[Ref.: #37641]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31304]			optimum	6
[Ref.: #31304]			growth	05-07

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31304]

Oxygen tolerance

aerobe

[Ref.: #31304]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31304]		NaCl		growth	<7	%
[Ref.: #31304]		NaCl		optimum	3.5	%

[Ref.: #67770]

Observation

quinones: MK-9, MK-10, MK-11, MK-12

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31304]	glycerol ChEBI	+	carbon source
[Ref.: #31304]	glycogen ChEBI	+	carbon source
[Ref.: #31304]	melibiose ChEBI	+	carbon source
[Ref.: #31304]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #31304]	raffinose ChEBI	+	carbon source

	Enzyme	Enzyme activity	EC number
[Ref.: #31304]	alkaline phosphatase	+	3.1.3.1
[Ref.: #31304]	catalase	+	1.11.1.6
[Ref.: #67770]	D-aminoacylase		3.5.1.14
[Ref.: #31304]	gelatinase	+
[Ref.: #31304]	pyrazinamidase	+	3.5.1.B15
[Ref.: #31304]	urease	+	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6869]	Sample type/isolated from	soil
[Ref.: #6869]	Geographic location (country and/or sea, region)	Natori, Miyagi
[Ref.: #6869]	Country	Japan
[Ref.: #6869]	Country ISO 3 Code	JPN
[Ref.: #6869]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	Natori, Miyagi Pref.
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6869]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6869]	Microbacterium natoriense strain TNJL143-2 16S ribosomal RNA gene, partial sequence	AY566291	1429	ENA	284570 tax ID

[Ref.: #6869]	GC-content	69.1 mol%
[Ref.: #67770]	GC-content	69.1 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #6869]

Culture collection no.

DSM 17277, ATCC BAA 1032, CIP 108753, JCM 12611

Literature:

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Purification, characterization, and primary structure of a novel N-acyl-D-amino acid amidohydrolase from Microbacterium natoriense TNJL143-2.	Liu J, Asano Y, Ikoma K, Yamashita S, Hirose Y, Shimoyama T, Takahashi S, Nakayama T, Nishino T	J Biosci Bioeng	10.1016/j.jbiosc.2012.05.015	2012	*
Phylogeny	Microbacterium ginsengiterrae sp. nov., a beta-glucosidase-producing bacterium isolated from soil of a ginseng field.	Kim YJ, Kim MK, Bui TPN, Kim HB, Srinivasan S, Yang DC	Int J Syst Evol Microbiol	10.1099/ijs.0.015784-0	2010	*
Phylogeny	Microbacterium natoriense sp. nov., a novel D-aminoacylase-producing bacterium isolated from soil in Natori, Japan.	Liu J, Nakayama T, Hemmi H, Asano Y, Tsuruoka N, Shimomura K, Nishijima M, Nishino T	Int J Syst Evol Microbiol	10.1099/ijs.0.63265-0	2005	*

References

#6869

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17277

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#31304

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27621 (see below)

#37641 ; Curators of the CIP;
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#27621

IJSEM 661 2005 ( DOI 10.1099/ijs.0.63265-0 , PubMed 15774640 )

* These data were automatically processed and therefore are not curated

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