Name: Microbacterium paraoxydans CF36
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7414

Type strain:

Species: Microbacterium paraoxydans

Strain Designation: CF36

Culture col. no.: DSM 15019, CCUG 46601, CIP 108082, NRRL B-24275, JCM 12372, IAM 15196, IFM 10517, LMG 23172, NBRC 103076, VTT E-072721

Strain history: CIP <- 2003, DSMZ <- G. Wauters: strain CF36 <- J. Gigi

NCBI tax ID(s): 1223529 (strain), 199592 (species)

SI-ID 113575

DSM 15019, CCUG 46601, CIP 108082, NRRL B-24275, JCM 12372, LMG 23172, DSM 15109, IAM 15196, NBRC 103076, VTT E-072721

Other strains from Microbacterium paraoxydans

M. paraoxydans CF7, DSM 15020, CCUG 46602
M. paraoxydans CF40, DSM 15021, CCUG 46603
M. paraoxydans C57-33, DSM 15461
M. paraoxydans KO_PS37, DSM 30839
M. paraoxydans CCUG 60603 A
M. paraoxydans T_C3, DSM 110318
M. paraoxydans DH1b, DSM 103224

Section

Microbacterium paraoxydans CF36 is an aerobe, mesophilic, Gram-positive human pathogen that was isolated from blood of a child with acute lymphoblastic leukemia.

Gram-positive
motile
rod-shaped
aerobe
human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Microbacteriaceae*
	Genus	*Microbacterium*
	Species	**Microbacterium paraoxydans**
	Full Scientific Name (LPSN)	Microbacterium paraoxydans Laffineur et al. 2003

Information on morphological and physiological properties Morphology

[Ref.: #121572]	Gram stain	positive

[Ref.: #121572]	Cell shape	rod-shaped

[Ref.: #121572]	Motility	yes

[Ref.: #57279]

Incubation period

1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5670]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #5670]

Culture medium growth

[Ref.: #5670]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5670]

Culture medium composition

expand / minimize

[Ref.: #5670]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #5670]

Culture medium growth

[Ref.: #5670]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5670]

Culture medium composition

expand / minimize

[Ref.: #40483]

Culture medium

MEDIUM 45 - for Columbia agar with sheep blood

[Ref.: #40483]

Culture medium growth

[Ref.: #40483]

Culture medium composition

expand / minimize

[Ref.: #121572]

Culture medium

CIP Medium 45

[Ref.: #121572]

Culture medium growth

[Ref.: #121572]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #121572]

Culture medium

CIP Medium 72

[Ref.: #121572]

Culture medium growth

[Ref.: #121572]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #5670]		growth	37 °C
[Ref.: #40483]		growth	37 °C
[Ref.: #57279]		growth	37 °C
[Ref.: #67770]		growth	37 °C
[Ref.: #121572]		growth	15-37 °C
[Ref.: #121572]	no	growth	10 °C
[Ref.: #121572]	no	growth	41 °C
[Ref.: #121572]	no	growth	45 °C

[Ref.: #5670]	Temperature range	mesophilic
[Ref.: #40483]	Temperature range	mesophilic
[Ref.: #57279]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #121572]	Temperature range	psychrophilic
[Ref.: #121572]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #57279]	Oxygen tolerance	aerobe
[Ref.: #121572]	Oxygen tolerance	obligate aerobe

	Salt	Tested relation		Salt conc.
[Ref.: #121572]	NaCl		growth	0-6	%
[Ref.: #121572]	NaCl	no	growth	8	%
[Ref.: #121572]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121572]	citrate ChEBI	-	carbon source
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121572]	esculin ChEBI	+	hydrolysis
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121572]	hippurate ChEBI	-	hydrolysis
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121572]	nitrate ChEBI	-	reduction
[Ref.: #121572]	nitrate ChEBI	-	respiration
[Ref.: #121572]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #121572]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite production	Metabolite	Production
[Ref.: #121572]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #121572]		acetoin ChEBI	-
[Ref.: #121572]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #121572]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	+	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #121572]	amylase	-
[Ref.: #121572]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121572]	caseinase	+	3.4.21.50
[Ref.: #121572]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #121572]	DNase	+
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #121572]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #121572]	gelatinase	+
[Ref.: #121572]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #121572]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121572]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121572]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #121572]	oxidase	-
[Ref.: #121572]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #121572]	protease	+
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #121572]	tryptophan deaminase	-
[Ref.: #121572]	tween esterase	-
[Ref.: #121572]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #121572]	11191	not determinedn.d.	+/-	-	+/-	-	-	-	-	-	-	+/-	+/-	+/-	+/-	-	-	-	-	+/-	-	-	-	-	-	-	+/-	-	+/-	+/-	-	-	+/-	+/-	-	+/-	-	-	-	-	+/-	+/-	-	-	-	+/-	-	-	+/-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121572]	10870	-	-	-	-	-	+	-	-	+	-	+	+	-	+	-	+	+	+	+	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5670]	Sample type/isolated from	blood of a child with acute lymphoblastic leukemia
[Ref.: #5670]	Country	Belgium
[Ref.: #5670]	Country ISO 3 Code	BEL
[Ref.: #5670]	Continent	Europe

[Ref.: #57279]	Sample type/isolated from	Human blood,5-yr-old boy,leukemia
[Ref.: #57279]	Sampling date	1997-03-01
[Ref.: #57279]	Country	Belgium
[Ref.: #57279]	Country ISO 3 Code	BEL
[Ref.: #57279]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Blood of a child with acute lymphoblastic leukemia

[Ref.: #121572]	Sample type/isolated from	Human, Blood
[Ref.: #121572]	Country	Belgium
[Ref.: #121572]	Country ISO 3 Code	BEL
[Ref.: #121572]	Continent	Europe
[Ref.: #121572]	Isolation date	1997
* marker position based on {}

Isolation sources categories

#Infection	#Patient	-
#Host	#Human	#Child
#Host Body Product	#Fluids	#Blood

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ491806 (>99% sequence identity) for Microbacterium from Microbeatlas

Aquatic Samples	9581
Soil Samples	10113
Animal Samples	16954
Plant Samples	5605
Total Samples	42253

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5670]	Pathogenicity (human)	yes, in single cases
[Ref.: #5670]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121572]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5670]	Microbacterium paraoxydans partial 16S rRNA gene, strain CF36	AJ491806	1490	ENA	199592 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Microbacterium paraoxydans NBRC 103076	GCA_001552495	contig	GenBank	1223529 tax ID
[Ref.: #66792]	Microbacterium paraoxydans DSM 15019	GCA_900105335	chromosome	GenBank	199592 tax ID	*
[Ref.: #66792]	Microbacterium paraoxydans NBRC 103076	1223529.4	wgs	PATRIC	1223529 tax ID	*
[Ref.: #66792]	Microbacterium paraoxydans strain DSM 15019	199592.7	complete	PATRIC	199592 tax ID	*
[Ref.: #66792]	Microbacterium paraoxydans DSM 15019	2634166897	draft	JGI IMG/M	199592 tax ID	*
[Ref.: #66792]	Microbacterium paraoxydans NBRC 103076	2731957588	draft	JGI IMG/M	1223529 tax ID	*

[Ref.: #5670]

GC-content

69.9 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Microbacterium paraoxydans strain DSM 15019 PATRIC: 199592.7
[Ref.: #69480]	motile	yes	59.447	no
[Ref.: #69480]	gram-positive	yes	90.576	no
[Ref.: #69480]	anaerobic	no	99.06	yes
[Ref.: #69480]	halophile	no	89.094	no
[Ref.: #69480]	spore-forming	no	85.497	no
[Ref.: #69480]	glucose-util	yes	81.267	no
[Ref.: #69480]	flagellated	no	91.432	no
[Ref.: #69480]	aerobic	yes	94.704	yes
[Ref.: #69480]	thermophile	no	99.483	yes
[Ref.: #69480]	glucose-ferment	no	89.826	no

Availability in culture collections External links

[Ref.: #5670]

Culture collection no.

DSM 15019, CCUG 46601, CIP 108082, NRRL B-24275, JCM 12372, IAM 15196, IFM 10517, LMG 23172, NBRC 103076, VTT E-072721

[Ref.: #76863]

SI-ID 113575

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Microbacterium oleivorans sp. nov. and Microbacterium hydrocarbonoxydans sp. nov., novel crude-oil-degrading Gram-positive bacteria.	Schippers A, Bosecker K, Sproer C, Schumann P	Int J Syst Evol Microbiol	10.1099/ijs.0.63305-0	2005	*
Phylogeny	Bacteremia due to a novel Microbacterium species in a patient with leukemia and description of Microbacterium paraoxydans sp. nov.	Laffineur K, Avesani V, Cornu G, Charlier J, Janssens M, Wauters G, Delmee M	J Clin Microbiol	10.1128/JCM.41.5.2242-2246.2003	2003	*

References

#5670

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15019

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#40483 ; Curators of the CIP;
#57279 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 46601

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76863

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID113575.1 )

#121572 Collection of Institut Pasteur ; Curators of the CIP; CIP 108082
* These data were automatically processed and therefore are not curated

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Microbacterium paraoxydans strain DSM 15019

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Change proposal