Leucobacter iarius (DSM 17402, CIP 108831, 40)

Name: Leucobacter iarius (DSM 17402, CIP 108831, 40)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7351

Type strain

Strain Designation 40

Culture col. no. DSM 17402 CIP 108831 40 JCM 14736

NCBI tax ID(s) 333963

Special Collections DSMZ JCM CIP Wink compendium Literature strains

History DSMZ <- Vishal Somvanshi, strain 40 JCM <-- CIP 108831 <-- DSM 17402 <-- V. S. Somvanshi 40. CIP <- 2005, DSMZ <- V.S. Somvanshi, India: strain 40

Links

Leucobacter iarius 40 is an obligate aerobe, Gram-positive, rod-shaped bacterium that was isolated from crushed infective juveniles of Steinernema thermophilum.

Gram-positive rod-shaped obligate aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 302762

CIP 108831,DSM 17402,JCM 14736

@ref 20215

Last LPSN update 2026-05-29 08:26:37

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Microbacteriaceae

Genus Leucobacter

Species Leucobacter iarius

Full scientific name Leucobacter iarius Somvanshi et al. 2007

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
31940	positive	1.66-2.91 µm	0.37-0.49 µm	rod-shaped
120036	positive			rod-shaped
125439	positive				95.493

Colony morphology

@ref	Colony color	Incubation period	Medium used	Hemolysis ability
19748	Cream (9001)	10-14 days	ISP 2
19748	Cream (9001)	10-14 days	ISP 3
19748	Cream (9001)	10-14 days	ISP 4
19748	Cream (9001)	10-14 days	ISP 5
19748	Cream (9001)	10-14 days	ISP 6
19748	Cream (9001)	10-14 days	ISP 7
120036				0

Multicellular morphology

@ref	Forms multicellular complex	Medium name
19748		ISP 2
19748		ISP 3
19748		ISP 4
19748		ISP 5
19748		ISP 6
19748		ISP 7

Pigmentation

31940

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Composition	Medium link
19748	ISP 2	Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19748	ISP 3	Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19748	ISP 4	Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19748	ISP 5	Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19748	ISP 6	Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19748	ISP 7	Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
34612	MEDIUM 72- for trypto casein soja agar	Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120036	CIP Medium 72		Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
19748	positive	optimum	28
31940	positive	growth	05-37
31940	positive	optimum	30
34612	positive	growth	30
67770	positive	growth	28
120036	positive	growth	15-41
120036	negative	growth	10

Culture pH

@ref	Ability	Type	PH	PH range
31940	positive	optimum	7
31940	positive	growth	05-09	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
120036	obligate aerobe
125439	obligate aerobe	97.759

Spore formation

31940

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
31940	NaCl	positive	growth	05-07 %
31940	NaCl	positive	optimum	6 %
120036	NaCl	positive	growth	0-10 %

Observation

67770

Observationquinones: MK-11, MK-10, MK-9

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
31940	58143 ChEBI	5-dehydro-D-gluconate	+	carbon source
31940	16449 ChEBI	alanine	+	carbon source
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
120036	16947 ChEBI	citrate	+	carbon source
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68371	17634 ChEBI	D-glucose	-	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68379	4853 ChEBI	esculin	+	hydrolysis	from API Coryne
120036	4853 ChEBI	esculin	+	hydrolysis
31940	28757 ChEBI	fructose	+	carbon source
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68379	5291 ChEBI	gelatin	-	hydrolysis	from API Coryne
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
31940	29987 ChEBI	glutamate	+	carbon source
31940	17754 ChEBI	glycerol	+	carbon source
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
120036	606565 ChEBI	hippurate	+	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
31940	21217 ChEBI	L-alaninamide	+	carbon source
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
31940	506227 ChEBI	N-acetylglucosamine	+	carbon source
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
120036	17632 ChEBI	nitrate	-	reduction
120036	17632 ChEBI	nitrate	-	respiration
120036	16301 ChEBI	nitrite	-	reduction
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
31940	17148 ChEBI	putrescine	+	carbon source
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
31940	33942 ChEBI	ribose	+	carbon source
31940	17814 ChEBI	salicin	+	carbon source
31940	17822 ChEBI	serine	+	carbon source
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68379	17992 ChEBI	sucrose	-	fermentation	from API Coryne
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
31940	17748 ChEBI	thymidine	+	carbon source
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
31940	53423 ChEBI	tween 40	+	carbon source
31940	53426 ChEBI	tween 80	+	carbon source
68379	16199 ChEBI	urea	+	hydrolysis	from API Coryne
31940	16704 ChEBI	uridine	+	carbon source
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
120036	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
120036	15688 ChEBI	acetoin	+
120036	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
120036	alcohol dehydrogenase	-	1.1.1.1
68379	alkaline phosphatase	+	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68379	alpha-glucosidase	-	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
120036	amylase	-
68382	beta-galactosidase	-	3.2.1.23	from API zym
120036	beta-galactosidase	-	3.2.1.23
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68382	beta-glucosidase	+	3.2.1.21	from API zym
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
120036	caseinase	-	3.4.21.50
120036	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
120036	DNase	-
68382	esterase (C 4)	-		from API zym
120036	gamma-glutamyltransferase	-	2.3.2.2
120036	gelatinase	-
68379	gelatinase	-		from API Coryne
120036	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
120036	lipase	-
68382	lipase (C 14)	-		from API zym
120036	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
120036	ornithine decarboxylase	-	4.1.1.17
120036	oxidase	-
120036	phenylalanine ammonia-lyase	-	4.3.1.24
68379	pyrazinamidase	+	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
68382	trypsin	-	3.4.21.4	from API zym
120036	tryptophan deaminase	-
120036	tween esterase	+
120036	urease	-	3.5.1.5
68379	urease	+	3.5.1.5	from API Coryne

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
19748	-	+	-	+	-	-	-	-	+	+	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
19748	not determinedn.d.	+	-	+	-	+	+	-	-	-	+	+	-	-	-	-	+	-	-	-
120036	-	-	-	-	-	+	-	-	-	-	+	+	-	-	-	-	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
120036	not determinedn.d.	+/-	-	-	-	+/-	-	-	-	-	-	-	+/-	-	-	-	-	+/-	-	-	-	-	+/-	-	+/-	+/-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Infection	#Disease	-
#Host	#Invertebrates (Other)	#Nematoda

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
6953	crushed infective juveniles of Steinernema thermophilum			India	IND	Asia
67770	Crushed infective juveniles of the entomopathogenic nematode Steinernema thermophilum collected from soil	Steinernema thermophilum	New Delhi	India	IND	Asia
120036	Animal, Entomopathogenic nematode, Steinernema thermophilum	Steinenema thermophilum		India	IND	Asia	2005

Taxonmaps

69479

Global distribution of 16S sequence AM040493 (>99% sequence identity) for Leucobacter iarius from Microbeatlas

Aquatic Samples	71
Soil Samples	22
Animal Samples	211
Plant Samples	11
Total Samples	315

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6953	1	Risk group (German classification) According to TRBA 466
19748	1	Risk group (German classification) According to TRBA 466
120036	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM3953010v1 assembly for Leucobacter iarius JCM 14736	scaffold	GCA_039530105	333963	46.33

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
6953	Leucobacter iarius 40 16S rRNA gene, type strain 40T	AM040493	1508		333963

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM3953010v1 assembly for Leucobacter iarius JCM 14736
NCBI: GCA_039530105

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.76	no
125439	gram_stain	BacteriaNetⓘ	positive	95.49	no
125439	motility	BacteriaNetⓘ	no	74.36	no
125439	spore_formation	BacteriaNetⓘ	no	81.76	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	Genome Sequencing of the Antibiotic-Resistant Leucobacter sp. HNU-1 and Its Developmental Toxicity in Caenorhabditis elegans.	Ju J, Lu X, Gao Z, Yin H, Xu S, Li H.	Int J Mol Sci	10.3390/ijms26083673	2025
Phylogeny	Leucobacter humi sp. nov., Isolated from Forest Soil.	Her J, Lee SS	Curr Microbiol	10.1007/s00284-015-0820-z	2015
Phylogeny	Leucobacter iarius sp. nov., in the family Microbacteriaceae.	Somvanshi VS, Lang E, Schumann P, Pukall R, Kroppenstedt RM, Ganguly S, Stackebrandt E	Int J Syst Evol Microbiol	10.1099/ijs.0.64683-0	2007

References

#6953	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17402
#19748	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#28196	IJSEM 682 2007 ( DOI 10.1099/ijs.0.64683-0 , PubMed 17392186 )
#31940	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28196
#34612	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120036	Collection of Institut Pasteur ; Curators of the CIP; CIP 108831
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Leucobacter iarius (DSM 17402, CIP 108831, 40)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API coryne

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_039530105

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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