Leifsonella bigeumensis (DSM 19322, KCTC 19268, MSL 27)

Name: Leifsonella bigeumensis (DSM 19322, KCTC 19268, MSL 27)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7343

Type strain

Strain Designation MSL-27

Culture col. no. DSM 19322 KCTC 19268 MSL 27 JCM 16949 CIP 109937 1 more

NCBI tax ID(s) 433643

Special Collections DSMZ JCM KCTC CIP Wink compendium Literature strains

History <- Chang-Jin Kim; MSL 27 KCTC 19268 <-- C.-J. Kim MSL-27. <- CJ Kim, KRIBB CIP <- 2009, KCTC <- C.-J. Kim, Functional Metabolomics Research Center, KRIBB, Daejeon 305-806, Republic of Korea: strain MSL-27

Links

Leifsonella bigeumensis MSL-27 is an aerobe, mesophilic, Gram-positive prokaryote that was isolated from soil, farming field.

Gram-positive motile ovoid-shaped aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 396569

KCTC 19268,JCM 16949,DSM 19322

@ref 20215

Last LPSN update 2026-02-09 11:19:27

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Microbacteriaceae

Genus Leifsonella

Species Leifsonella bigeumensis

Full scientific name Leifsonella bigeumensis (Dastager et al. 2008) Gtari et al. 2025

Synonyms (1)

Leifsonia bigeumensis

Morphology

Cell morphology

@ref	Gram stain	Cell shape
32489	positive	ovoid-shaped
67771	positive
116815	positive	rod-shaped

Colony morphology

@ref	Colony color	Incubation period	Medium used
19781	Rapeseed yellow (1021)	10-14 days	ISP 2
19781	Rapeseed yellow (1021)	10-14 days	ISP 3
19781		10-14 days	ISP 4
19781		10-14 days	ISP 5
19781	Rapeseed yellow (1021)	10-14 days	ISP 6
19781	Rapeseed yellow (1021)	10-14 days	ISP 7

Multicellular morphology

@ref	Forms multicellular complex	Medium name
19781		ISP 2
19781		ISP 3
19781		ISP 4
19781		ISP 5
19781		ISP 6
19781		ISP 7

Pigmentation

32489

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8037	R2A MEDIUM (DSMZ Medium 830)	Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
19781	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19781	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19781	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19781	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19781	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19781	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
116815	CIP Medium 566	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
8037	positive	growth	28	mesophilic
19781	positive	optimum	28	mesophilic
32489	positive	growth	20-37
32489	positive	optimum	28	mesophilic
67770	positive	growth	28	mesophilic
67771	positive	growth	30	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
32489	positive	optimum	8
32489	positive	growth	06-12	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
32489	aerobe
67771	aerobe

Spore formation

32489

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
32489	NaCl	positive	growth	01-03 %
32489	NaCl	positive	optimum	01-03 %

Observation

@ref	Observation
32489	aggregates in chains
67770	quinones: MK-11

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
32489	17057 ChEBI	cellobiose	+	carbon source
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	-	hydrolysis	from API Coryne
68379	5291 ChEBI	gelatin	+	hydrolysis	from API Coryne
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
32489	29864 ChEBI	mannitol	+	carbon source
32489	28053 ChEBI	melibiose	+	carbon source
32489	17632 ChEBI	nitrate	+	reduction
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
116815	17632 ChEBI	nitrate	+	reduction
116815	16301 ChEBI	nitrite	-	reduction
32489	16634 ChEBI	raffinose	+	carbon source
32489	26546 ChEBI	rhamnose	+	carbon source
68379	17992 ChEBI	sucrose	+	fermentation	from API Coryne
32489	27082 ChEBI	trehalose	+	carbon source
68379	16199 ChEBI	urea	+	hydrolysis	from API Coryne

Metabolite production

@ref	Chebi-ID	Metabolite	Production
116815	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68379	alkaline phosphatase	+	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68382	beta-glucosidase	-	3.2.1.21	from API zym
68379	beta-glucosidase	-	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
116815	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68379	gelatinase	+		from API Coryne
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
116815	oxidase	-
68379	pyrazinamidase	+	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	+	3.4.19.3	from API Coryne
68382	trypsin	-	3.4.21.4	from API zym
116815	urease	-	3.5.1.5
68379	urease	+	3.5.1.5	from API Coryne

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
19781	-	+	+	+	-	-	+	-	-	+	+	not determinedn.d.	-	-	-	-	-	-	+	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
19781	not determinedn.d.	+	+	+	-	+	+	-	-	-	+	-	-	-	-	+	-	+	-	-
116815	-	-	-	-	-	+	-	-	-	-	+	+	-	-	-	+	-	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Agriculture	#Field
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
8037	soil, farming field	Bigeum Island	Republic of Korea	KOR	Asia
67770	Soil from Bigeum Island		Republic of Korea	KOR	Asia
67771	From farming field	Bikeum island	Republic of Korea	KOR	Asia
116815	Environment, Soil, farming field	Bigeum Island	Republic of Korea	KOR	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8037	1	Risk group (German classification) According to TRBA 466
19781	1	Risk group (German classification) According to TRBA 466
116815	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM3953726v1 assembly for Leifsonella bigeumensis JCM 16949	scaffold	GCA_039537265	433643	44.74

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
8037	Leifsonia bigeumensis strain MSL-27 16S ribosomal RNA gene, partial sequence	EF466124	1491		433643

GC content

@ref	GC-content (mol%)	Method
8037	69
67770	69	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Taxonomic rearrangement of Salinibacterium, Leifsonia, Diaminobutyricibacter, Antiquaquibacter, Homoserinimonas and Glaciibacter: refining genus boundaries and proposal of two new genera - Orlajensenia gen. nov. and Leifsonella gen. nov.	Gtari M, Boussoufa D, Ghodhbane-Gtari F, Sbissi I.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006809	2025
Phylogeny	Homoserinimonas aerilata gen. nov., sp. nov., a novel member of the family Microbacteriaceae isolated from an air sample in Korea.	Kim SJ, Jang YH, Hamada M, Tamura T, Ahn JH, Weon HY, Suzuki K, Kwon SW.	J Microbiol	10.1007/s12275-012-2096-3	2012
Phylogeny	Leifsonia bigeumensis sp. nov., isolated from soil on Bigeum Island, Korea.	Dastager SG, Lee JC, Ju YJ, Park DJ, Kim CJ	Int J Syst Evol Microbiol	10.1099/ijs.0.65572-0	2008

References

#8037	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19322
#19781	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#28709	IJSEM 1935 2008 ( DOI 10.1099/ijs.0.65572-0 , PubMed 18676484 )
#32489	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28709
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#116815	Collection of Institut Pasteur ; Curators of the CIP; CIP 109937
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Leifsonella bigeumensis (DSM 19322, KCTC 19268, MSL 27)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Enzymes

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_039537265

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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