Name: Leifsonia poae CCUG 47140
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7342

Type strain:

Species: Leifsonia poae

Culture col. no.: CCUG 47140, DSM 15202, CIP 106654, VKM Ac-1401, JCM 11952, IAM 15136, KCTC 19442, NBRC 103069

Strain history: CIP <- 2000, L. Evtushenko, VKM, Moscow, Russia: strain Ac-1401

NCBI tax ID(s): 110933 (species)

SI-ID 72045

VKM Ac-1401, CIP 106654, DSM 15202, JCM 11952, CCUG 47140, IAM 15136, NBRC 103069

Other strains from Leifsonia poae

L. poae CCUG 42970

Section

Leifsonia poae CCUG 47140 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from nematode gall on Poa annua root.

Gram-positive
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Microbacteriaceae*
	Genus	*Leifsonia*
	Species	**Leifsonia poae**
	Full Scientific Name (LPSN)	Leifsonia poae Evtushenko et al. 2000

Information on morphological and physiological properties Morphology

[Ref.: #121916]	Gram stain	positive

[Ref.: #121916]	Cell shape	rod-shaped

[Ref.: #121916]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5755]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #5755]

Culture medium growth

[Ref.: #5755]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5755]

Culture medium composition

expand / minimize

[Ref.: #35974]

Culture medium

MEDIUM 111 - for Clavibacter

[Ref.: #35974]

Culture medium growth

[Ref.: #35974]

Culture medium composition

expand / minimize

[Ref.: #121916]

Culture medium

CIP Medium 111

[Ref.: #121916]

Culture medium growth

[Ref.: #121916]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #5755]		growth	28 °C
[Ref.: #35974]		growth	20 °C
[Ref.: #57533]		growth	30 °C
[Ref.: #67770]		growth	28 °C
[Ref.: #121916]		growth	15-30 °C
[Ref.: #121916]	no	growth	10 °C
[Ref.: #121916]	no	growth	37 °C
[Ref.: #121916]	no	growth	41 °C
[Ref.: #121916]	no	growth	45 °C

[Ref.: #5755]	Temperature range	mesophilic
[Ref.: #35974]	Temperature range	psychrophilic
[Ref.: #57533]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #121916]	Temperature range	psychrophilic
[Ref.: #121916]	Temperature range	mesophilic
[Ref.: #121916]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #57533]	Oxygen tolerance	aerobe
[Ref.: #121916]	Oxygen tolerance	obligate aerobe

	Salt	Tested relation		Salt conc.
[Ref.: #121916]	NaCl		growth	0-2	%
[Ref.: #121916]	NaCl	no	growth	4	%
[Ref.: #121916]	NaCl	no	growth	6	%
[Ref.: #121916]	NaCl	no	growth	8	%
[Ref.: #121916]	NaCl	no	growth	10	%

[Ref.: #5755]	Murein short key	B07
[Ref.: #5755]	Murein types	B2gamma {Gly} [L-Dab] D-Glu-D-Dab

[Ref.: #67770]

Observation

quinones: MK-11, MK-10, MK-12

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #121916]	citrate ChEBI	-	carbon source
[Ref.: #121916]	esculin ChEBI	+	hydrolysis
[Ref.: #121916]	hippurate ChEBI	-	hydrolysis
[Ref.: #121916]	nitrate ChEBI	-	reduction
[Ref.: #121916]	nitrate ChEBI	-	respiration
[Ref.: #121916]	nitrite ChEBI	-	reduction

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #121916]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite production	Metabolite	Production
[Ref.: #121916]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #121916]		acetoin ChEBI	-
[Ref.: #121916]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #121916]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	+	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121916]	amylase	-
[Ref.: #121916]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121916]	caseinase	-	3.4.21.50
[Ref.: #121916]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #121916]	DNase	+
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #121916]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #121916]	gelatinase	-
[Ref.: #121916]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #121916]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121916]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121916]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #121916]	oxidase	+
[Ref.: #121916]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #121916]	protease	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #121916]	tryptophan deaminase	-
[Ref.: #121916]	tween esterase	-
[Ref.: #121916]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #121916]	1762	not determinedn.d.	+	+	+	+	+	-	-	+	-	+	+	-	+	-	-	+	+	+	+	+	+	+	+	+	-	+	-	-	-	-	-	+	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+	-	-	-	+	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121916]	9703	-	-	+	+	-	+	+	-	-	-	+	+	+	+	-	+	+	+	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5755]	Sample type/isolated from	nematode gall on Poa annua root
[Ref.: #5755]	Host species	Poa annua
[Ref.: #5755]	Country	Russia
[Ref.: #5755]	Country ISO 3 Code	RUS

[Ref.: #57533]	Sample type/isolated from	Poa annua root gall
[Ref.: #57533]	Sampling date	1993
[Ref.: #57533]	Geographic location (country and/or sea, region)	Moscow region
[Ref.: #57533]	Country	Russia
[Ref.: #57533]	Country ISO 3 Code	RUS
[Ref.: #57533]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Poa annua root galls induced by the grass root gall nematode Subanguina radicicola
[Ref.: #67770]	Host species	Subanguina radicicola
[Ref.: #67770]	Geographic location (country and/or sea, region)	Moscow Region
[Ref.: #67770]	Country	Russia
[Ref.: #67770]	Country ISO 3 Code	RUS

[Ref.: #121916]	Sample type/isolated from	Plant, Poa annua root gall
[Ref.: #121916]	Geographic location (country and/or sea, region)	Moscow region
[Ref.: #121916]	Country	Russian Federation
[Ref.: #121916]	Country ISO 3 Code	RUS
[Ref.: #121916]	Continent	Europe
[Ref.: #121916]	Isolation date	1993
* marker position based on {}

Isolation sources categories

#Host	#Invertebrates (Other)	#Nematoda
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Infection	#Plant infections	#Gall
#Host Body-Site	#Plant	#Root (Rhizome)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AM410682 (>99% sequence identity) for Leifsonia from Microbeatlas

Aquatic Samples	554
Soil Samples	2218
Animal Samples	672
Plant Samples	396
Total Samples	3840

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5755]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121916]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5755]	Leifsonia poae 16S ribosomal RNA, partial sequence	AF116342	1488	ENA	110933 tax ID
[Ref.: #20218]	Leifsonia poae strain VKM Ac-1401 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	DQ232613	2111	ENA	110933 tax ID	*
[Ref.: #20218]	Leifsonia poae partial 16S rRNA gene, type strain DSM 15202	AM410682	1488	ENA	110933 tax ID	*

Availability in culture collections External links

[Ref.: #5755]

Culture collection no.

CCUG 47140, DSM 15202, CIP 106654, VKM Ac-1401, JCM 11952, IAM 15136, KCTC 19442, NBRC 103069

[Ref.: #76792]

SI-ID 72045

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Rudaibacter terrae gen. nov., sp. nov., isolated from greenhouse soil.	Kim SJ, Moon JY, Hamada M, Tamura T, Weon HY, Suzuki KI, Kwon SW	Int J Syst Evol Microbiol	10.1099/ijs.0.049817-0	2013	*
Phylogeny	Leifsonia kribbensis sp. nov., isolated from soil.	Dastager SG, Lee JC, Ju YJ, Park DJ, Kim CJ	Int J Syst Evol Microbiol	10.1099/ijs.0.001925-0	2009	*
Phylogeny	Leifsonia ginsengi sp. nov., isolated from ginseng root.	Qiu F, Huang Y, Sun L, Zhang X, Liu Z, Song W	Int J Syst Evol Microbiol	10.1099/ijs.0.64487-0	2007	*
Phylogeny	Leifsonia poae gen. nov., sp. nov., isolated from nematode galls on Poa annua, and reclassification of 'Corynebacterium aquaticum' Leifson 1962 as Leifsonia aquatica (ex Leifson 1962) gen. nov., nom. rev., comb. nov. and Clavibacter xyli Davis et al. 1984 with two subspecies as Leifsonia xyli (Davis et al. 1984) gen. nov., comb. nov.	Evtushenko LI, Dorofeeva LV, Subbotin SA, Cole JR, Tiedje JM	Int J Syst Evol Microbiol	10.1099/00207713-50-1-371	2000	*

References

#5755

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15202

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#35974 ; Curators of the CIP;
#57533 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 47140
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#76792

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID72045.1 )

#121916 Collection of Institut Pasteur ; Curators of the CIP; CIP 106654
* These data were automatically processed and therefore are not curated

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