Leifsonia aquatica (DSM 20146, ATCC 14665, NCIB 9460)

Name: Leifsonia aquatica (DSM 20146, ATCC 14665, NCIB 9460)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7339

Type strain

Strain Designation 150

Culture col. no. DSM 20146 ATCC 14665 NCIB 9460 CCUG 27700 CCTM La 2040 15 more

NCBI tax ID(s) 1358026 144185

Special Collections DSMZ CCUG JCM KCTC CIP Wink compendium

History <- ATCC <- E. Leifson, 150 (Corynebacterium aquaticum) 1964, E. Leifson: strain 150 K. Suzuki CNF 038 <-- AJ 1413 <-- ATCC 14665 <-- E. Leifson 150. CIP <- 1964, E. Leifson: strain 150

Links

Leifsonia aquatica 150 is a bacterium that was isolated from distilled water.

genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 389265

ATCC 14665,JCM 1368,DSM 20146,NCIB 9460,VKM Ac-1400,CCUG 27700,CCRC 11574,IAM 12441,NCIMB 9460,IFO 15710,IFO 12154,CECT 535,KCTC 9098,KCTC 9233,NBRC 12154,NBRC 15710,BCRC 11574,CCT 3023,CIP 64.13

@ref 20215

Last LPSN update 2026-02-09 11:19:27

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Microbacteriaceae

Genus Leifsonia

Species Leifsonia aquatica

Full scientific name Leifsonia aquatica (ex Leifson 1962) Evtushenko et al. 2000

Synonyms (2)

"Leifsonia aquatica"
"Corynebacterium aquaticum"

BacDive ID	Other strains from **Leifsonia aquatica** (31)	Type strain
103259	L. aquatica ST033330(HKI),
137980	L. aquatica 46.87, CIP 102793
143992	L. aquatica CCUG 22294, ATCC 51722
145394	L. aquatica CCUG 28357
145396	L. aquatica CCUG 28359
145397	L. aquatica CCUG 28360
145398	L. aquatica CCUG 28361
145408	L. aquatica CCUG 28381
145827	L. aquatica CCUG 29729, ATCC 51721
146058	L. aquatica CCUG 30288
146168	L. aquatica CCUG 30492
146482	L. aquatica CCUG 31268, ATCC 51723
146531	L. aquatica CCUG 31381
146652	L. aquatica CCUG 31729
146887	L. aquatica CCUG 32370
147006	L. aquatica CCUG 32634
147048	L. aquatica CCUG 32739
147135	L. aquatica CCUG 32923
147138	L. aquatica CCUG 32928
147139	L. aquatica CCUG 32929
147243	L. aquatica CCUG 33173
147572	L. aquatica CCUG 33823
147616	L. aquatica CCUG 33903
147756	L. aquatica CCUG 34181
147808	L. aquatica CCUG 34265
147809	L. aquatica CCUG 34266
148446	L. aquatica CCUG 35456 A
149443	L. aquatica CCUG 37759
150257	L. aquatica CCUG 39403
150423	L. aquatica CCUG 39879
150425	L. aquatica CCUG 39881

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	positive	99.5

Colony morphology

@ref	Colony color	Incubation period	Medium used
18306	Cream (9001)	10-14 days	ISP 2
18306	Lemon yellow (1012)	10-14 days	ISP 3
18306	Lemon yellow (1012)	10-14 days	ISP 4
18306		10-14 days	ISP 5
18306	Lemon yellow (1012)	10-14 days	ISP 6
18306		10-14 days	ISP 7

Multicellular morphology

@ref	Forms multicellular complex	Medium name
18306		ISP 2
18306		ISP 3
18306		ISP 4
18306		ISP 5
18306		ISP 6
18306		ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8570	CORYNEBACTERIUM AGAR (DSMZ Medium 53)	Medium recipe at MediaDive	Name: CORYNEBACTERIUM AGAR (DSMZ Medium 53) Composition: Agar 15.0 g/l Casein peptone 10.0 g/l NaCl 5.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled water
18306	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18306	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18306	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18306	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18306	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18306	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
37611	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
116431	CIP Medium 3	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
8570	positive	growth	30
18306	positive	optimum	28
37611	positive	growth	30
67770	positive	growth	30

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	96.8

Halophily

@ref	Salt	Growth	Tested relation	Concentration
18306	NaCl	positive	maximum	5 %

Murein

@ref	Murein short key	Type
8570	B07	B2gamma {Gly} [L-Dab] D-Glu-D-Dab

Observation

67770

Observationquinones: MK-11, MK-10

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
18306	22599 ChEBI	arabinose	+
18306	62968 ChEBI	cellulose	+
68368	16947 ChEBI	citrate	-	assimilation	from API 20E
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	+	hydrolysis	from API Coryne
18306	28757 ChEBI	fructose	+
68379	5291 ChEBI	gelatin	-	hydrolysis	from API Coryne
68368	5291 ChEBI	gelatin	-	hydrolysis	from API 20E
18306	17234 ChEBI	glucose	+
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
18306	29864 ChEBI	mannitol	+
18306	17268 ChEBI	myo-inositol	-
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
18306	16634 ChEBI	raffinose	-
18306	26546 ChEBI	rhamnose	+
18306	17992 ChEBI	sucrose	+
68379	17992 ChEBI	sucrose	+	fermentation	from API Coryne
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68379	16199 ChEBI	urea	+	hydrolysis	from API Coryne
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E
18306	18222 ChEBI	xylose	+

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	+		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68379	alkaline phosphatase	+	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	+	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68379	beta-galactosidase	+	3.2.1.23	from API Coryne
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68382	beta-glucosidase	+	3.2.1.21	from API zym
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
68379	gelatinase	-		from API Coryne
68368	gelatinase	-		from API 20E
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	+		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
68379	pyrazinamidase	+	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
68382	trypsin	-	3.4.21.4	from API zym
68379	urease	+	3.5.1.5	from API Coryne
68368	urease	+	3.5.1.5	from API 20E
68382	valine arylamidase	+		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	cellulose degradation	100	5 of 5
66794	teichoic acid biosynthesis	100	1 of 1
66794	palmitate biosynthesis	100	22 of 22
66794	starch degradation	100	10 of 10
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	cardiolipin biosynthesis	100	7 of 7
66794	coenzyme A metabolism	100	4 of 4
66794	formaldehyde oxidation	100	3 of 3
66794	pentose phosphate pathway	100	11 of 11
66794	adipate degradation	100	2 of 2
66794	glycogen metabolism	100	5 of 5
66794	phenylacetate degradation (aerobic)	100	5 of 5
66794	vitamin K metabolism	100	5 of 5
66794	metabolism of amino sugars and derivatives	100	5 of 5
66794	phenylmercury acetate degradation	100	2 of 2
66794	ppGpp biosynthesis	100	4 of 4
66794	acetate fermentation	100	4 of 4
66794	tetrahydrofolate metabolism	100	14 of 14
66794	folate polyglutamylation	100	1 of 1
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	methylglyoxal degradation	100	5 of 5
66794	ketogluconate metabolism	100	8 of 8
66794	threonine metabolism	100	10 of 10
66794	suberin monomers biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	lipoate biosynthesis	100	5 of 5
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	ribulose monophosphate pathway	100	2 of 2
66794	phenylalanine metabolism	92.31	12 of 13
66794	valine metabolism	88.89	8 of 9
66794	d-mannose degradation	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	serine metabolism	88.89	8 of 9
66794	peptidoglycan biosynthesis	86.67	13 of 15
66794	leucine metabolism	84.62	11 of 13
66794	NAD metabolism	83.33	15 of 18
66794	pyrimidine metabolism	82.22	37 of 45
66794	glutamate and glutamine metabolism	82.14	23 of 28
66794	metabolism of disaccharids	81.82	9 of 11
66794	purine metabolism	80.85	76 of 94
66794	Entner Doudoroff pathway	80	8 of 10
66794	flavin biosynthesis	80	12 of 15
66794	alanine metabolism	79.31	23 of 29
66794	tryptophan metabolism	78.95	30 of 38
66794	photosynthesis	78.57	11 of 14
66794	citric acid cycle	78.57	11 of 14
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	aspartate and asparagine metabolism	77.78	7 of 9
66794	glycolysis	76.47	13 of 17
66794	sulfopterin metabolism	75	3 of 4
66794	gluconeogenesis	75	6 of 8
66794	butanoate fermentation	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	isoleucine metabolism	75	6 of 8
66794	dTDPLrhamnose biosynthesis	75	6 of 8
66794	heme metabolism	71.43	10 of 14
66794	propionate fermentation	70	7 of 10
66794	4-hydroxyphenylacetate degradation	70	7 of 10
66794	myo-inositol biosynthesis	70	7 of 10
66794	vitamin B1 metabolism	69.23	9 of 13
66794	histidine metabolism	68.97	20 of 29
66794	degradation of sugar alcohols	68.75	11 of 16
66794	non-pathway related	68.42	26 of 38
66794	degradation of pentoses	67.86	19 of 28
66794	arginine metabolism	66.67	16 of 24
66794	cyanate degradation	66.67	2 of 3
66794	molybdenum cofactor biosynthesis	66.67	6 of 9
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	degradation of hexoses	66.67	12 of 18
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	enterobactin biosynthesis	66.67	2 of 3
66794	lipid metabolism	64.52	20 of 31
66794	d-xylose degradation	63.64	7 of 11
66794	proline metabolism	63.64	7 of 11
66794	C4 and CAM-carbon fixation	62.5	5 of 8
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	oxidative phosphorylation	61.54	56 of 91
66794	phenol degradation	60	12 of 20
66794	gallate degradation	60	3 of 5
66794	methionine metabolism	57.69	15 of 26
66794	ubiquinone biosynthesis	57.14	4 of 7
66794	propanol degradation	57.14	4 of 7
66794	lysine metabolism	57.14	24 of 42
66794	polyamine pathway	56.52	13 of 23
66794	degradation of sugar acids	56	14 of 25
66794	cysteine metabolism	55.56	10 of 18
66794	isoprenoid biosynthesis	53.85	14 of 26
66794	urea cycle	53.85	7 of 13
66794	3-phenylpropionate degradation	53.33	8 of 15
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	glycine metabolism	50	5 of 10
66794	pantothenate biosynthesis	50	3 of 6
66794	CMP-KDO biosynthesis	50	2 of 4
66794	androgen and estrogen metabolism	50	8 of 16
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	tyrosine metabolism	50	7 of 14
66794	lactate fermentation	50	2 of 4
66794	cyclohexanol degradation	50	2 of 4
66794	carotenoid biosynthesis	50	11 of 22
66794	quinate degradation	50	1 of 2
66794	ethanol fermentation	50	1 of 2
66794	kanosamine biosynthesis II	50	1 of 2
66794	phosphatidylethanolamine bioynthesis	46.15	6 of 13
66794	vitamin B6 metabolism	45.45	5 of 11
66794	4-hydroxymandelate degradation	44.44	4 of 9
66794	reductive acetyl coenzyme A pathway	42.86	3 of 7
66794	glutathione metabolism	42.86	6 of 14
66794	arachidonate biosynthesis	40	2 of 5
66794	O-antigen biosynthesis	40	2 of 5
66794	ethylmalonyl-CoA pathway	40	2 of 5
66794	D-cycloserine biosynthesis	40	2 of 5
66794	bile acid biosynthesis, neutral pathway	35.29	6 of 17
66794	IAA biosynthesis	33.33	1 of 3
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	sphingosine metabolism	33.33	2 of 6
66794	lipid A biosynthesis	33.33	3 of 9
66794	arachidonic acid metabolism	33.33	6 of 18
66794	ascorbate metabolism	31.82	7 of 22
66794	benzoyl-CoA degradation	28.57	2 of 7
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	biotin biosynthesis	25	1 of 4
66794	toluene degradation	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	catecholamine biosynthesis	25	1 of 4
66794	phenylpropanoid biosynthesis	23.08	3 of 13
66794	chlorophyll metabolism	22.22	4 of 18
66794	nitrate assimilation	22.22	2 of 9
66794	daunorubicin biosynthesis	22.22	2 of 9

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
18306	-	not determinedn.d.	not determinedn.d.	-	-	-	+	not determinedn.d.	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
18306	-	+	-	+	-	+	+	+	+	+	-	not determinedn.d.	-	-	-	-	-	-	+	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
18306	not determinedn.d.	-	+	-	+	+	+	+	-	-	+	+	+	+	-	+	+	+	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	-
#Engineered	#Industrial	#Engineered product
#Engineered	#Treatment	#Sterilized (Desinfected)

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent	Isolation date
8570	distilled water
48900	Distilled water
67770	Distilled water
116431	Environment, Distilled water	France	FRA	Europe	1962

Taxonmaps

69479

Global distribution of 16S sequence X77450 (>99% sequence identity) for Leifsonia from Microbeatlas

Aquatic Samples	123
Soil Samples	509
Animal Samples	333
Plant Samples	123
Total Samples	1088

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8570	1	Risk group (German classification) According to TRBA 466
18306	1	Risk group (German classification) According to TRBA 466
116431	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1419077v1 assembly for Leifsonia aquatica DSM 20146	contig	GCA_014190775	144185.6	2821241899	144185	68.64
67770	ASM46948v1 assembly for Leifsonia aquatica ATCC 14665	scaffold	GCA_000469485	1358026.3	2602041580	1358026

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Leifsonia aquatica 16S ribosomal RNA gene, partial sequence	AF299364	435	144185
8570	C.aquaticum (DSM 20146) 16S rRNA gene	X77450	1475	144185
67770	Leifsonia aquatica gene for 16S ribosomal RNA	D45057	1468	144185
67770	Leifsonia aquatica strain VKM Ac-1400 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	DQ232618	2111	144185
124043	Leifsonia aquatica strain ATCC 14665 16S ribosomal RNA gene, partial sequence.	MW228036	1328	144185

GC content

@ref	GC-content (mol%)	Method
8570	69.3
67770	73.2	thermal denaturation, midpoint method (Tm)
67770	69.3	thermal denaturation, midpoint method (Tm)
67770	70.3	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Leifsonia aquatica ATCC 14665
NCBI: GCA_000469485

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	52.90	no
125439	motility	BacteriaNetⓘ	no	78.40	no
125439	gram_stain	BacteriaNetⓘ	positive	99.50	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	96.80	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Leifsonia aquatica ATCC 14665
PATRIC: 1358026.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	85.31	no
125438	anaerobic	anaerobicⓘ	no	96.05	no
125438	spore-forming	spore-formingⓘ	no	69.45	no
125438	aerobic	aerobicⓘ	yes	86.63	no
125438	thermophilic	thermophileⓘ	no	96.30	yes
125438	flagellated	motile2+ⓘ	no	73.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Precision Probiotics in Agroecosystems: Multiple Strategies of Native Soil Microbiotas for Conquering the Competitor Ralstonia solanacearum.	Yin J, Zhang Z, Guo Y, Chen Y, Xu Y, Chen W, Shao Y, Yu Y, Zhu L, Chen L, Ruan L.	mSystems	10.1128/msystems.01159-21	2022
	Bacteraemia due to Microbacterium paraoxydans in a patient with chronic kidney disease, refractory hypertension and sarcoidosis.	Chorost MS, Smith NC, Hutter JN, Ong AC, Stam JA, McGann PT, Hinkle MK, Schaecher KE, Kamau E.	JMM Case Rep	10.1099/jmmcr.0.005169	2018
Enzymology	Stabilization of Multimeric Proteins via Intersubunit Cyclization.	Zhu L, Wang S, Tian W, Zhang Y, Song Y, Zhang J, Mu B, Peng C, Deng Z, Ma H, Qu X.	Appl Environ Microbiol	10.1128/aem.01239-17	2017
Genetics	Microbial isolation and characterization from two flex lines from the urine processor assembly onboard the international space station.	Nguyen HN, Sharp GM, Stahl-Rommel S, Velez Justiniano YA, Castro CL, Nelman-Gonzalez M, O'Rourke A, Lee MD, Williamson J, McCool C, Crucian B, Clark KW, Jain M, Castro-Wallace SL.	Biofilm	10.1016/j.bioflm.2023.100108	2023
Phylogeny	Identification of clinical coryneform bacterial isolates: comparison of biochemical methods and sequence analysis of 16S rRNA and rpoB genes.	Adderson EE, Boudreaux JW, Cummings JR, Pounds S, Wilson DA, Procop GW, Hayden RT.	J Clin Microbiol	10.1128/jcm.01849-07	2008
Enzymology	Use of tuf sequences for genus-specific PCR detection and phylogenetic analysis of 28 streptococcal species.	Picard FJ, Ke D, Boudreau DK, Boissinot M, Huletsky A, Richard D, Ouellette M, Roy PH, Bergeron MG.	J Clin Microbiol	10.1128/jcm.42.8.3686-3695.2004	2004
Enzymology	Rapid concentration and molecular enrichment approach for sensitive detection of Escherichia coli and Shigella species in potable water samples.	Maheux AF, Bissonnette L, Boissinot M, Bernier JL, Huppe V, Picard FJ, Berube E, Bergeron MG.	Appl Environ Microbiol	10.1128/aem.02337-10	2011
Pathogenicity	New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci.	Huletsky A, Giroux R, Rossbach V, Gagnon M, Vaillancourt M, Bernier M, Gagnon F, Truchon K, Bastien M, Picard FJ, van Belkum A, Ouellette M, Roy PH, Bergeron MG.	J Clin Microbiol	10.1128/jcm.42.5.1875-1884.2004	2004
	Antibiotic-resistant bacteria aerosol in a Caribbean coastal city: Pre- and post- COVID-19 lockdown.	Morgado-Gamero WB, Hernandez L, Medina J, De Moya I, Gallego-Cartagena E, Parody A, Agudelo-Castaneda D.	Sci Total Environ	10.1016/j.scitotenv.2024.178158	2025
	A Rare Surgical Site Infection Caused by Leifsonia aquatica (Non-diphtheric Corynebacterium) in an Immunocompetent Patient: A Case Report From North India.	Goyal A, Singhal S, Kumar V, Shakya P, Goyal S.	Cureus	10.7759/cureus.70295	2024
	Polyhexamethylene guanidine hydrochloride as promising active ingredient for oral antiseptic products to eliminate microorganisms threatening the health of endangered wild cats: a comparative study with chlorhexidine digluconate.	de Barros RA, Rodrigues MA, Ambrosio SR, SolaVeneziani RC, Junior DP, Parreira RLT, Ambrosio MALV, de Souza Silva T, de Freitas Pereira L, Pessinato MG, da Silva Cardoso de Brito VJ, Branco CH, Dias FGG.	Braz J Microbiol	10.1007/s42770-023-01107-x	2023
Genetics	Metagenomic Insight into the Associated Microbiome in Plasmodia of Myxomycetes.	Peng X, Li S, Dou W, Li M, Gontcharov AA, Peng Z, Qi B, Wang Q, Li Y.	Microorganisms	10.3390/microorganisms12122540	2024
	Leifsonia aquatica: Case report and literature review.	De Carvalho RV, Santos CS, Dos Santos Sant'Anna LS, Lima FF, Junior RH, Grabois MF, Mattos-Guaraldi AL.	Mol Clin Oncol	10.3892/mco.2021.2387	2021
	Leifsonia Species Bacteremia in a Hemodialysis Patient: A Difficult-to-Identify Organism.	Al-Sardi M, Radwan H, Itbaileh AB, AlMusa Z.	Cureus	10.7759/cureus.17994	2021
Genetics	Genomic epidemiology of a novel Pandoraea pneumonica group caused severe bloodstream infection in Hainan, China, 2021-2024.	Chen C, Wang M, Huang T, Huang DL, Yu S, Zhao HM, Fu XX, Li XX, Wu H.	Front Cell Infect Microbiol	10.3389/fcimb.2025.1560634	2025
Enzymology	Crystal structure of a putative short-chain dehydrogenase/reductase from Paraburkholderia xenovorans.	Davidson J, Nicholas K, Young J, Conrady DG, Mayclin S, Subramanian S, Staker BL, Myler PJ, Asojo OA.	Acta Crystallogr F Struct Biol Commun	10.1107/s2053230x21012632	2022
Metabolism	Fusion of an alcohol dehydrogenase with an aminotransferase using a PAS linker to improve coupled enzymatic alcohol-to-amine conversion.	Lerchner A, Daake M, Jarasch A, Skerra A.	Protein Eng Des Sel	10.1093/protein/gzw039	2016
Phylogeny	Selection and evaluation of Malaysian Bacillus spp. strains as potential probiotics in cultured tiger grouper (Epinephelus fuscoguttatus).	Yasin IM, Razak NF, Natrah FMI, Harmin SA.	J Environ Biol		2016
	Peritonitis caused by Haemophilus parainfluenzae, Leifsonia aquatica, and Gordonia spp. in a patient undergoing continuous ambulatory peritoneal dialysis.	Gardenier JC, Sawyer RG, Sifri CD, Brayman K, Wispelway B, Bonatti H.	Surg Infect (Larchmt)	10.1089/sur.2011.009	2012
Enzymology	Catheter-associated bloodstream infection caused by Leifsonia aquatica in a haemodialysis patient: a case report.	Porte L, Soto A, Andrighetti D, Dabanch J, Braun S, Saldivia A, Flores JC, Wozniak A, Garcia P, Weitzel T.	J Med Microbiol	10.1099/jmm.0.037457-0	2012
Enzymology	Septicemia caused by Leifsonia aquatica in a healthy patient after retinal reattachment surgery.	Han L, Lei JE, Wang X, Guo LT, Kang QY, He L, Xu JR.	J Clin Microbiol	10.1128/jcm.01339-13	2013
Genetics	The dynamics of the midgut microbiome in Aedes aegypti during digestion reveal putative symbionts.	Salgado JFM, Premkrishnan BNV, Oliveira EL, Vettath VK, Goh FG, Hou X, Drautz-Moses DI, Cai Y, Schuster SC, Junqueira ACM.	PNAS Nexus	10.1093/pnasnexus/pgae317	2024
Enzymology	Central venous catheter-associated Leifsonia aquatica bacteremia in a hemodialysis-dependent patient.	Sulpher J, Desjardins M, Lee BC.	Diagn Microbiol Infect Dis	10.1016/j.diagmicrobio.2007.12.012	2008
	Crystal structures of bacterial small multidrug resistance transporter EmrE in complex with structurally diverse substrates.	Kermani AA, Burata OE, Koff BB, Koide A, Koide S, Stockbridge RB.	Elife	10.7554/elife.76766	2022
	[From the Mailing List SIN: Epidemic of infections caused by 'aquatic' bacteria in patients undergoing hemodialysis via central venous catheters].	D'Amico M, Mangano S, Spinelli M, Sala E, Vigano' EF, Grilli R, Fraticelli M, Grillo C, Limido A.	G Ital Nefrol		2005
Pathogenicity	Antibiotic Resistance of Airborne Viable Bacteria and Size Distribution in Neonatal Intensive Care Units.	Morgado-Gamero WB, Mendoza Hernandez M, Castillo Ramirez M, Medina-Altahona J, De La Hoz S, Posso Mendoza H, Parody A, Teixeira EC, Agudelo-Castaneda DM.	Int J Environ Res Public Health	10.3390/ijerph16183340	2019
Enzymology	Specific and functional diversity of endophytic bacteria from pine wood nematode Bursaphelenchus xylophilus with different virulence.	Wu XQ, Yuan WM, Tian XJ, Fan B, Fang X, Ye JR, Ding XL.	Int J Biol Sci	10.7150/ijbs.5071	2013
Pathogenicity	High-resolution taxonomic examination of the oral microbiome after oil pulling with standardized sunflower seed oil and healthy participants: a pilot study.	Griessl T, Zechel-Gran S, Olejniczak S, Weigel M, Hain T, Domann E.	Clin Oral Investig	10.1007/s00784-020-03582-0	2021
Metabolism	Coupled reactions by coupled enzymes: alcohol to lactone cascade with alcohol dehydrogenase-cyclohexanone monooxygenase fusions.	Aalbers FS, Fraaije MW.	Appl Microbiol Biotechnol	10.1007/s00253-017-8501-4	2017
Enzymology	Molecular identification of clinical "difficult-to-identify" microbes from sequencing 16S ribosomal DNA and internal transcribed spacer 2.	Cheng C, Sun J, Zheng F, Wu K, Rui Y.	Ann Clin Microbiol Antimicrob	10.1186/1476-0711-13-1	2014
Transcriptome	Pitchers of Nepenthes khasiana express several digestive-enzyme encoding genes, harbor mostly fungi and probably evolved through changes in the expression of leaf polarity genes.	Dkhar J, Bhaskar YK, Lynn A, Pareek A.	BMC Plant Biol	10.1186/s12870-020-02663-2	2020
Enzymology	Corynebacterium prosthetic joint infection.	Cazanave C, Greenwood-Quaintance KE, Hanssen AD, Patel R.	J Clin Microbiol	10.1128/jcm.06439-11	2012
	Assessment of microbial diversity in biofilms recovered from endotracheal tubes using culture dependent and independent approaches.	Vandecandelaere I, Matthijs N, Van Nieuwerburgh F, Deforce D, Vosters P, De Bus L, Nelis HJ, Depuydt P, Coenye T.	PLoS One	10.1371/journal.pone.0038401	2012
Enzymology	Identification of bacteria on the surface of clinically infected and non-infected prosthetic hip joints removed during revision arthroplasties by 16S rRNA gene sequencing and by microbiological culture.	Dempsey KE, Riggio MP, Lennon A, Hannah VE, Ramage G, Allan D, Bagg J.	Arthritis Res Ther	10.1186/ar2201	2007
	Phase I/II Trial of StemRegenin-1 Expanded Umbilical Cord Blood Hematopoietic Stem Cells Supports Testing as a Stand-Alone Graft.	Wagner JE, Brunstein CG, Boitano AE, DeFor TE, McKenna D, Sumstad D, Blazar BR, Tolar J, Le C, Jones J, Cooke MP, Bleul CC.	Cell Stem Cell	10.1016/j.stem.2015.10.004	2016
Metabolism	Purification and characterization of a novel alcohol dehydrogenase from Leifsonia sp. strain S749: a promising biocatalyst for an asymmetric hydrogen transfer bioreduction.	Inoue K, Makino Y, Itoh N.	Appl Environ Microbiol	10.1128/aem.71.7.3633-3641.2005	2005
	Genomic Characterization of Piscicolin CM22 Produced by Carnobacterium maltaromaticum CM22 Strain Isolated from Salmon (Salmo salar).	Gonzalez-Gragera E, Garcia-Lopez JD, Teso-Perez C, Jimenez-Hernandez I, Peralta-Sanchez JM, Valdivia E, Montalban-Lopez M, Martin-Platero AM, Banos A, Martinez-Bueno M.	Probiotics Antimicrob Proteins	10.1007/s12602-024-10316-1	2025
	Leifsonia gen. nov., a genus for 2,4-diaminobutyric acid-containing actinomycetes to accommodate "Corynebacterium aquaticum" Leifson 1962 and Clavibacter xyli subsp. cynodontis Davis et al. 1984.	Suzuki KI, Suzuki M, Sasaki J, Park YH, Komagata KK	J Gen Appl Microbiol	10.2323/jgam.45.253	1999
Phylogeny	Humibacter albus gen. nov., sp. nov., isolated from sewage sludge compost.	Vaz-Moreira I, Nobre MF, Ferreira AC, Schumann P, Nunes OC, Manaia CM.	Int J Syst Evol Microbiol	10.1099/ijs.0.65266-0	2008
Phylogeny	Rudaibacter terrae gen. nov., sp. nov., isolated from greenhouse soil.	Kim SJ, Moon JY, Hamada M, Tamura T, Weon HY, Suzuki KI, Kwon SW	Int J Syst Evol Microbiol	10.1099/ijs.0.049817-0	2013
Phylogeny	Leifsonia kribbensis sp. nov., isolated from soil.	Dastager SG, Lee JC, Ju YJ, Park DJ, Kim CJ	Int J Syst Evol Microbiol	10.1099/ijs.0.001925-0	2009
Phylogeny	Leifsonia poae gen. nov., sp. nov., isolated from nematode galls on Poa annua, and reclassification of 'Corynebacterium aquaticum' Leifson 1962 as Leifsonia aquatica (ex Leifson 1962) gen. nov., nom. rev., comb. nov. and Clavibacter xyli Davis et al. 1984 with two subspecies as Leifsonia xyli (Davis et al. 1984) gen. nov., comb. nov.	Evtushenko LI, Dorofeeva LV, Subbotin SA, Cole JR, Tiedje JM	Int J Syst Evol Microbiol	10.1099/00207713-50-1-371	2000

References

#8570	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20146
#18306	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#37611	; Curators of the CIP;
#48900	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 27700
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#116431	Collection of Institut Pasteur ; Curators of the CIP; CIP 64.13
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Leifsonia aquatica (DSM 20146, ATCC 14665, NCIB 9460)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

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Physiology and metabolism

Oxygen tolerance

Halophily

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API 20E

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API zym

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