Name: Curtobacterium pusillum 100
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7317

Type strain:

Species: Curtobacterium pusillum

Strain Designation: 100

Culture col. no.: DSM 20527, ATCC 19096, IAM 1479, NCIB 10354, JCM 1350, BCRC 10376, CGMCC 1.1905, CIP 81.24, IFO 12674, IMET 11077, KCCM 12168, KCTC 3492, LMG 8788, NBRC 12674, NCIMB 10354, VKM Ac-1413, VKM Ac-2099

Strain history: CIP <- 1981, IAM <- H. Iizuka: strain 100, Brevibacterium pusillum

NCBI tax ID(s): 69373 (species)

SI-ID 10472

LMG 8788, ATCC 19096, DSM 20527, IAM 1479, IFO 12674, JCM 1350, NCIB 10354, IMET 11077, CCRC 10376, NCIMB 10354, VKM Ac-1413, VKM Ac-2099, CIP 81.24, KCTC 3492, NBRC 12674, BCRC 10376, CGMCC 1.1905, NCPPB 4113

Other strains from Curtobacterium pusillum

C. pusillum 106, DSM 20529, ATCC 19097, IAM ...
C. pusillum CCUG 51725
C. pusillum CCUG 51729
C. pusillum JCM 3881

Section

Curtobacterium pusillum 100 is a mesophilic, Gram-positive bacterium that was isolated from oil brine.

Gram-positive
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Microbacteriaceae*
	Genus	*Curtobacterium*
	Species	**Curtobacterium pusillum**
	Full Scientific Name (LPSN)	Curtobacterium pusillum (Iizuka and Komagata 1965) Yamada and Komagata 1972 (Approved Lists 1980)

Synonym

Brevibacterium pusillum

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #18446]	Colony color	Ivory (1014)
[Ref.: #18446]	Incubation period	10-14 days
[Ref.: #18446]	Cultivation medium used	ISP 2

[Ref.: #18446]	Colony color	Light ivory (1015)
[Ref.: #18446]	Incubation period	10-14 days
[Ref.: #18446]	Cultivation medium used	ISP 3

[Ref.: #18446]	Colony color	Colorless
[Ref.: #18446]	Incubation period	10-14 days
[Ref.: #18446]	Cultivation medium used	ISP 4

[Ref.: #18446]	Colony color	Colorless
[Ref.: #18446]	Incubation period	10-14 days
[Ref.: #18446]	Cultivation medium used	ISP 5

[Ref.: #18446]	Colony color	Colorless
[Ref.: #18446]	Incubation period	10-14 days
[Ref.: #18446]	Cultivation medium used	ISP 6

[Ref.: #18446]	Colony color	Light ivory (1015)
[Ref.: #18446]	Incubation period	10-14 days
[Ref.: #18446]	Cultivation medium used	ISP 7

[Ref.: #18446]	Multicellular complex forming ability	no
[Ref.: #18446]	Medium Name (multicellularity)	ISP 2

[Ref.: #18446]	Multicellular complex forming ability	no
[Ref.: #18446]	Medium Name (multicellularity)	ISP 3

[Ref.: #18446]	Multicellular complex forming ability	no
[Ref.: #18446]	Medium Name (multicellularity)	ISP 4

[Ref.: #18446]	Multicellular complex forming ability	no
[Ref.: #18446]	Medium Name (multicellularity)	ISP 5

[Ref.: #18446]	Multicellular complex forming ability	no
[Ref.: #18446]	Medium Name (multicellularity)	ISP 6

[Ref.: #18446]	Multicellular complex forming ability	no
[Ref.: #18446]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #18446]

Culture medium

ISP 2

[Ref.: #18446]

Culture medium growth

[Ref.: #18446]

Culture medium composition

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[Ref.: #18446]

Culture medium

ISP 3

[Ref.: #18446]

Culture medium growth

[Ref.: #18446]

Culture medium composition

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[Ref.: #18446]

Culture medium

ISP 4

[Ref.: #18446]

Culture medium growth

[Ref.: #18446]

Culture medium composition

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[Ref.: #18446]

Culture medium

ISP 5

[Ref.: #18446]

Culture medium growth

[Ref.: #18446]

Culture medium composition

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[Ref.: #18446]

Culture medium

ISP 6

[Ref.: #18446]

Culture medium growth

[Ref.: #18446]

Culture medium composition

expand / minimize

[Ref.: #18446]

Culture medium

ISP 7

[Ref.: #18446]

Culture medium growth

[Ref.: #18446]

Culture medium composition

expand / minimize

[Ref.: #8894]

Culture medium

CORYNEBACTERIUM AGAR (DSMZ Medium 53)

[Ref.: #8894]

Culture medium growth

[Ref.: #8894]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8894]

Culture medium composition

expand / minimize

[Ref.: #37719]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #37719]

Culture medium growth

[Ref.: #37719]

Culture medium composition

expand / minimize

[Ref.: #122066]

Culture medium

CIP Medium 3

[Ref.: #122066]

Culture medium growth

[Ref.: #122066]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #8894]	growth	30 °C
[Ref.: #18446]	optimum	28 °C
[Ref.: #37719]	growth	30 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #8894]	Temperature range	mesophilic
[Ref.: #18446]	Temperature range	mesophilic
[Ref.: #37719]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	no Confidence in %94
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.898

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #18446]		NaCl		maximum	7.5	%

[Ref.: #8894]	Murein short key	B05
[Ref.: #8894]	Murein types	B2ß {Gly} [L-Hsr] D-Glu-D-Orn

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #18446]	arabinose ChEBI	+
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #18446]	cellulose ChEBI	-
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #18446]	fructose ChEBI	-
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E, API Coryne
[Ref.: #18446]	glucose ChEBI	+
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #18446]	mannitol ChEBI	-
[Ref.: #18446]	myo-inositol ChEBI	+
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #18446]	raffinose ChEBI	+
[Ref.: #18446]	rhamnose ChEBI	+
[Ref.: #18446]	sucrose ChEBI	+
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E, API Coryne
[Ref.: #18446]	xylose ChEBI	+
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	-		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym, API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	+	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym, API Coryne, API 20E
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym, API Coryne
[Ref.: #68379]	beta-glucuronidase	+	3.2.1.31	* from API Coryne
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne, API 20E
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym, API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #68379]	pyrazinamidase	+	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne, API 20E
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #18446]	36	+	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #18446]	123	-	+	+	+	+	+	+	+	+	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18446]	158	not determinedn.d.	+	+	+	-	+	+	+	+	-	+	+	+	+	-	+	+	+	+	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8894]	Sample type/isolated from	oil brine

[Ref.: #67770]	Sample type/isolated from	Oil-brine in Yabase oil-field in Akita Pref.
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia

[Ref.: #122066]	Sample type/isolated from	Oil brine
[Ref.: #122066]	Geographic location (country and/or sea, region)	Akita
[Ref.: #122066]	Country	Japan
[Ref.: #122066]	Country ISO 3 Code	JPN
[Ref.: #122066]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Contamination	#Oil (Fuel)
#Condition	#Saline	-
#Engineered	#Food production	#Oil (Food)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LN681569 (>99% sequence identity) for Curtobacterium from Microbeatlas

Aquatic Samples	6793
Soil Samples	11124
Animal Samples	21247
Plant Samples	9659
Total Samples	48823

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8894]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #18446]	Biosafety level	1
[Ref.: #122066]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8894]	Curtobacterium pusillum partial 16S rRNA gene, type strain DSM 20527T	LN681569	1474	ENA	69373 tax ID
[Ref.: #20218]	Curtobacterium pusillum 16S-23S ribosomal RNA intergenic spacer region, partial sequence	AY191510	634	ENA	69373 tax ID	*
[Ref.: #20218]	Curtobacterium pusillum 16S rRNA gene, type strain DSM 20527	AJ784400	1509	ENA	69373 tax ID	*
[Ref.: #20218]	Curtobacterium sp. IFO 12674T gene for 16S rRNA, partial sequence	AB046364	1506	ENA	133417 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Curtobacterium pusillum ATCC 19096	GCA_013359865	contig	GenBank	69373 tax ID
[Ref.: #66792]	Curtobacterium pusillum strain ATCC 19096	69373.17	wgs	PATRIC	69373 tax ID	*

[Ref.: #8894]	GC-content	69.0 mol%
[Ref.: #67770]	GC-content	74.2 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	69 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Curtobacterium pusillum strain ATCC 19096 PATRIC: 69373.7
[Ref.: #69481]	spore-forming	no	94	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Curtobacterium pusillum strain ATCC 19096 PATRIC: 69373.17
[Ref.: #69480]	gram-positive	yes	92.662	no
[Ref.: #69480]	anaerobic	no	99.282	no
[Ref.: #69480]	halophile	no	90.833	no
[Ref.: #69480]	spore-forming	no	93.443	no
[Ref.: #69480]	glucose-util	yes	87.56	yes
[Ref.: #69480]	thermophile	no	99.236	yes
[Ref.: #69480]	flagellated	no	93.766	no
[Ref.: #69480]	motile	yes	54.081	no
[Ref.: #69480]	aerobic	yes	91.402	no
[Ref.: #69480]	glucose-ferment	no	87.635	yes

Availability in culture collections External links

[Ref.: #8894]

Culture collection no.

DSM 20527, ATCC 19096, IAM 1479, NCIB 10354, JCM 1350, BCRC 10376, CGMCC 1.1905, CIP 81.24, IFO 12674, IMET 11077, KCCM 12168, KCTC 3492, LMG 8788, NBRC 12674, NCIMB 10354, VKM Ac-1413, VKM Ac-2099

[Ref.: #76767]

SI-ID 10472

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Curtobacterium ginsengisoli sp. nov., isolated from soil of a ginseng field.	Kim MK, Kim YJ, Kim HB, Kim SY, Yi TH, Yang DC	Int J Syst Evol Microbiol	10.1099/ijs.0.65591-0	2008	*
Phylogeny	Nucleic acid hybridization studies on Microbacterium, Curtobacterium, Agromyces and related taxa.	Dopfer H, Stackebrandt E, Fiedler F	J Gen Microbiol	10.1099/00221287-128-8-1697	1982	*

References

#8894

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20527

#18446

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#37719 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76767

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID10472.1 )

#122066 Collection of Institut Pasteur ; Curators of the CIP; CIP 81.24
* These data were automatically processed and therefore are not curated

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Curtobacterium pusillum strain ATCC 19096

Curtobacterium pusillum strain ATCC 19096

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