Curtobacterium pusillum 100 is a bacterium that was isolated from oil brine.
genome sequence 16S sequence Bacteria
SI-ID 10472
| @ref 20215 |
|
Last LPSN update
2026-02-09 11:19:26 |
| Domain Bacteria |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Micrococcales |
| Family Microbacteriaceae |
| Genus Curtobacterium |
| Species Curtobacterium pusillum |
| Full scientific name Curtobacterium pusillum (Iizuka and Komagata 1965) Yamada and Komagata 1972 (Approved Lists 1980) |
| Synonyms (1) |
| BacDive ID | Other strains from Curtobacterium pusillum (5) | Type strain |
|---|---|---|
| 7318 | C. pusillum 106, DSM 20529, ATCC 19097, IAM 1489, JCM ... | |
| 153944 | C. pusillum CCUG 51725 | |
| 153948 | C. pusillum CCUG 51729 | |
| 164864 | C. pusillum JCM 3881 | |
| 176420 | C. pusillum NiuAA3, ZK5344, AA3, DSM 114566 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 18446 | ISP 2 | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |||
| 18446 | ISP 3 | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |||
| 18446 | ISP 4 | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |||
| 18446 | ISP 5 | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |||
| 18446 | ISP 6 | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 18446 | ISP 7 | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 8894 | CORYNEBACTERIUM AGAR (DSMZ Medium 53) | Medium recipe at MediaDive  | Name: CORYNEBACTERIUM AGAR (DSMZ Medium 53) Composition: Agar 15.0 g/l Casein peptone 10.0 g/l NaCl 5.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled water | ||
| 37719 | MEDIUM 3 - Columbia agar | Columbia agar (39.000 g);distilled water (1000.000 ml) | |||
| 122066 | CIP Medium 3 | Medium recipe at CIP |
| @ref | Oxygen tolerance | Confidence | |
|---|---|---|---|
| 125439 | obligate aerobe | 94.4 |
| @ref | Salt | Growth | Tested relation | Concentration | |
|---|---|---|---|---|---|
| 18446 | NaCl | positive | maximum | 7.5 % |
| @ref | Murein short key | Type | |
|---|---|---|---|
| 8894 | B05 | B2ß {Gly} [L-Hsr] D-Glu-D-Orn |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 18446 | 22599 ChEBI | arabinose | + | ||
| 68368 | 29016 ChEBI | arginine | - | hydrolysis | from API 20E |
| 18446 | 62968 ChEBI | cellulose | - | ||
| 68368 | 16947 ChEBI | citrate | - | assimilation | from API 20E |
| 68379 | 17634 ChEBI | D-glucose | - | fermentation | from API Coryne |
| 68379 | 16899 ChEBI | D-mannitol | - | fermentation | from API Coryne |
| 68379 | 16988 ChEBI | D-ribose | - | fermentation | from API Coryne |
| 68379 | 65327 ChEBI | D-xylose | - | fermentation | from API Coryne |
| 68379 | 4853 ChEBI | esculin | + | hydrolysis | from API Coryne |
| 18446 | 28757 ChEBI | fructose | - | ||
| 68379 | 5291 ChEBI | gelatin | - | hydrolysis | from API Coryne |
| 68368 | 5291 ChEBI | gelatin | - | hydrolysis | from API 20E |
| 18446 | 17234 ChEBI | glucose | + | ||
| 68379 | 28087 ChEBI | glycogen | - | fermentation | from API Coryne |
| 68379 | 17716 ChEBI | lactose | - | fermentation | from API Coryne |
| 68368 | 25094 ChEBI | lysine | - | degradation | from API 20E |
| 68379 | 17306 ChEBI | maltose | - | fermentation | from API Coryne |
| 18446 | 29864 ChEBI | mannitol | - | ||
| 18446 | 17268 ChEBI | myo-inositol | + | ||
| 68379 | 17632 ChEBI | nitrate | - | reduction | from API Coryne |
| 68368 | 18257 ChEBI | ornithine | - | degradation | from API 20E |
| 18446 | 16634 ChEBI | raffinose | + | ||
| 18446 | 26546 ChEBI | rhamnose | + | ||
| 18446 | 17992 ChEBI | sucrose | + | ||
| 68379 | 17992 ChEBI | sucrose | - | fermentation | from API Coryne |
| 68368 | 27897 ChEBI | tryptophan | - | energy source | from API 20E |
| 68379 | 16199 ChEBI | urea | - | hydrolysis | from API Coryne |
| 68368 | 16199 ChEBI | urea | - | hydrolysis | from API 20E |
| 18446 | 18222 ChEBI | xylose | + |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68382 | acid phosphatase | + | 3.1.3.2 | from API zym |
| 68382 | alkaline phosphatase | + | 3.1.3.1 | from API zym |
| 68379 | alkaline phosphatase | + | 3.1.3.1 | from API Coryne |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | + | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | + | 3.2.1.22 | from API zym |
| 68382 | alpha-glucosidase | + | 3.2.1.20 | from API zym |
| 68379 | alpha-glucosidase | + | 3.2.1.20 | from API Coryne |
| 68382 | alpha-mannosidase | + | 3.2.1.24 | from API zym |
| 68368 | arginine dihydrolase | - | 3.5.3.6 | from API 20E |
| 68382 | beta-galactosidase | + | 3.2.1.23 | from API zym |
| 68379 | beta-galactosidase | + | 3.2.1.23 | from API Coryne |
| 68368 | beta-galactosidase | + | 3.2.1.23 | from API 20E |
| 68382 | beta-glucosidase | + | 3.2.1.21 | from API zym |
| 68379 | beta-glucosidase | + | 3.2.1.21 | from API Coryne |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 68379 | beta-glucuronidase | + | 3.2.1.31 | from API Coryne |
| 68382 | cystine arylamidase | + | 3.4.11.3 | from API zym |
| 68382 | esterase (C 4) | + | from API zym | |
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 68379 | gelatinase | - | from API Coryne | |
| 68368 | gelatinase | - | from API 20E | |
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 68382 | lipase (C 14) | - | from API zym | |
| 68368 | lysine decarboxylase | - | 4.1.1.18 | from API 20E |
| 68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 | from API zym |
| 68379 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 | from API Coryne |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | from API zym | |
| 68368 | ornithine decarboxylase | - | 4.1.1.17 | from API 20E |
| 68379 | pyrazinamidase | + | 3.5.1.B15 | from API Coryne |
| 68379 | pyrrolidonyl arylamidase | + | 3.4.19.3 | from API Coryne |
| 68382 | trypsin | + | 3.4.21.4 | from API zym |
| 68368 | tryptophan deaminase | - | 4.1.99.1 | from API 20E |
| 68379 | urease | - | 3.5.1.5 | from API Coryne |
| 68368 | urease | - | 3.5.1.5 | from API 20E |
| 68382 | valine arylamidase | + | from API zym |
| Cat1 | Cat2 | Cat3 | |
|---|---|---|---|
| #Engineered | #Contamination | #Oil (Fuel) | |
| #Condition | #Saline | - | |
| #Engineered | #Food production | #Oil (Food) |
Global distribution of 16S sequence LN681569 (>99% sequence identity) for Curtobacterium from Microbeatlas 
 Aquatic Samples |
6793 |
 Soil Samples |
11124 |
 Animal Samples |
21247 |
 Plant Samples |
9659 |
| Total Samples | 48823 |
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 124043 | ASM3953515v1 assembly for Curtobacterium pusillum JCM 1350 | contig | GCA_039535155   | 69373 | 62.63 | | ||
| 124043 | ASM4242894v1 assembly for Curtobacterium pusillum JCM 1350 | contig | GCA_042428945 | 69373 | 60.37 | | ||
| 67770 | ASM1335986v1 assembly for Curtobacterium pusillum ATCC 19096 | contig | GCA_013359865 | 69373.17  | 8068727991  | 69373 | 56.21 | |
| 124043 | ASM2792210v1 assembly for Curtobacterium pusillum | scaffold | GCA_027922105 | 69373.23 | 69373 | |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 20218 | Curtobacterium pusillum 16S-23S ribosomal RNA intergenic spacer region, partial sequence | AY191510 | 634 | | 69373 | |
| 20218 | Curtobacterium pusillum 16S rRNA gene, type strain DSM 20527 | AJ784400 | 1509 |  | 69373 | |
| 20218 | Curtobacterium pusillum gene for 16S rRNA, partial sequence | AB046364 | 1506 | | 133417 | |
| 8894 | Curtobacterium pusillum partial 16S rRNA gene, type strain DSM 20527T | LN681569 | 1474 | | 69373 | |
| 124043 | Curtobacterium pusillum strain JCM 1350 16S ribosomal RNA gene, partial sequence. | MT760355 | 1300 | | 69373 | |
| Topic | Title | Authors | Journal | DOI | Year | |
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| Native Cultivable Bacteria from the Blueberry Microbiome as Novel Potential Biocontrol Agents. | Chacon FI, Sineli PE, Mansilla FI, Pereyra MM, Diaz MA, Volentini SI, Poehlein A, Meinhardt F, Daniel R, Dib JR. | Microorganisms | 10.3390/microorganisms10050969 | 2022 | | |
| Bacterial Isolates Derived from Nest Soil Affect the Attraction and Digging Behavior of Workers of the Red Imported Fire Ant, Solenopsis invicta Buren. | Travanty NV, Vargo EL, Schal C, Apperson CS, Ponnusamy L. | Insects | 10.3390/insects13050444 | 2022 | | |
| Genetics | Prophage-Derived Regions in Curtobacterium Genomes: Good Things, Small Packages. | Evseev P, Lukianova A, Tarakanov R, Tokmakova A, Popova A, Kulikov E, Shneider M, Ignatov A, Miroshnikov K. | Int J Mol Sci | 10.3390/ijms24021586 | 2023 | |
| Phylogeny | First description of Curtobacterium spp. isolated from human clinical specimens. | Funke G, Aravena-Roman M, Frodl R. | J Clin Microbiol | 10.1128/jcm.43.3.1032-1036.2005 | 2005 | |
| Growth-Promoting Effects and Mechanisms of Synthetic Plant Growth-Promoting Rhizobacteria on Maize Seedlings. | Yu S, Mao M, Zhang H, Song H, Sun Y. | Microorganisms | 10.3390/microorganisms13112460 | 2025 | | |
| Insights into Endophytic and Rhizospheric Bacteria of Five Sugar Beet Hybrids in Terms of Their Diversity, Plant-Growth Promoting, and Biocontrol Properties. | Petrovic M, Janakiev T, Grbic ML, Unkovic N, Stevic T, Vukicevic S, Dimkic I. | Microb Ecol | 10.1007/s00248-023-02329-0 | 2023 | | |
| Evaluation of ready-to-use freezer stocks of a synthetic microbial community for maize root colonization. | Parnell JJ, Vintila S, Tang C, Wagner MR, Kleiner M. | Microbiol Spectr | 10.1128/spectrum.02401-23 | 2024 | | |
| Uncovering microbiomes of the rice phyllosphere using long-read metagenomic sequencing. | Masuda S, Gan P, Kiguchi Y, Anda M, Sasaki K, Shibata A, Iwasaki W, Suda W, Shirasu K. | Commun Biol | 10.1038/s42003-024-05998-w | 2024 | | |
| Quantification of the Composition Dynamics of a Maize Root-associated Simplified Bacterial Community and Evaluation of Its Biological Control Effect. | Niu B, Kolter R. | Bio Protoc | 10.21769/bioprotoc.2885 | 2018 | | |
| Expanded Substrate Specificity in D-Amino Acid Transaminases: A Case Study of Transaminase from Blastococcus saxobsidens | Shilova S, Matyuta I, Petrova E, Nikolaeva A, Rakitina T, Minyaev M, Boyko K, Popov V, Bezsudnova E. | Int J Mol Sci | 2023 | | ||
| Enzymology | Discovery and structural characterisation of new fold type IV-transaminases exemplify the diversity of this enzyme fold. | Pavkov-Keller T, Strohmeier GA, Diepold M, Peeters W, Smeets N, Schurmann M, Gruber K, Schwab H, Steiner K. | Sci Rep | 10.1038/srep38183 | 2016 | |
| Phylogeny | Exploring the diversity and dynamic of bacterial community vertically distributed in Tongguling National Nature Reserve in Hainan Island, China. | Li P, Wu H, Jin Y, Huang X, Chen Y, Yang X, Wang R, Zhang W. | Braz J Microbiol | 10.1007/s42770-019-00078-2 | 2019 | |
| Microbial Interactions Within Multiple-Strain Biological Control Agents Impact Soil-Borne Plant Disease. | Niu B, Wang W, Yuan Z, Sederoff RR, Sederoff H, Chiang VL, Borriss R. | Front Microbiol | 10.3389/fmicb.2020.585404 | 2020 | | |
| Occupational exposure to organic dust, microorganisms, endotoxin and peptidoglycan among plants processing workers in Poland. | Gora A, Mackiewicz B, Krawczyk P, Golec M, Skorska C, Sitkowska J, Cholewa G, Larsson L, Jarosz M, Wojcik-Fatla A, Dutkiewicz J. | Ann Agric Environ Med | 2009 | | ||
| Metabolism | Metabolic bifunctionality of Rv0812 couples folate and peptidoglycan biosynthesis in Mycobacterium tuberculosis. | Black KA, Duan L, Mandyoli L, Selbach BP, Xu W, Ehrt S, Sacchettini JC, Rhee KY. | J Exp Med | 10.1084/jem.20191957 | 2021 | |
| Enzymology | To the Understanding of Catalysis by D-Amino Acid Transaminases: A Case Study of the Enzyme from Aminobacterium colombiense. | Shilova SA, Khrenova MG, Matyuta IO, Nikolaeva AY, Rakitina TV, Klyachko NL, Minyaev ME, Boyko KM, Popov VO, Bezsudnova EY. | Molecules | 10.3390/molecules28052109 | 2023 | |
| Bacterial Flora of Freshwater Prawn, Macrobrachium rosenbergii(de Man), Cultured in Concrete Tanks in Saudi Arabia | Al-Harbi AH. | J Appl Aquac | 10.1300/j028v14n01_08 | 2003 | | |
| Phylogeny | Specialized metabolic functions of keystone taxa sustain soil microbiome stability. | Xun W, Liu Y, Li W, Ren Y, Xiong W, Xu Z, Zhang N, Miao Y, Shen Q, Zhang R. | Microbiome | 10.1186/s40168-020-00985-9 | 2021 | |
| Metabolism | The Uncommon Active Site of D-Amino Acid Transaminase from Haliscomenobacter hydrossis: Biochemical and Structural Insights into the New Enzyme. | Bakunova AK, Nikolaeva AY, Rakitina TV, Isaikina TY, Khrenova MG, Boyko KM, Popov VO, Bezsudnova EY. | Molecules | 10.3390/molecules26165053 | 2021 | |
| Microbial Community and Function-Based Synthetic Bioinoculants: A Perspective for Sustainable Agriculture. | Suman A, Govindasamy V, Ramakrishnan B, Aswini K, SaiPrasad J, Sharma P, Pathak D, Annapurna K. | Front Microbiol | 10.3389/fmicb.2021.805498 | 2021 | | |
| Oral Osteomicrobiology: The Role of Oral Microbiota in Alveolar Bone Homeostasis. | Cheng X, Zhou X, Liu C, Xu X. | Front Cell Infect Microbiol | 10.3389/fcimb.2021.751503 | 2021 | | |
| Enzymology | Simplified and representative bacterial community of maize roots. | Niu B, Paulson JN, Zheng X, Kolter R. | Proc Natl Acad Sci U S A | 10.1073/pnas.1616148114 | 2017 | |
| Enzymology | Nematicidal bacteria associated to pinewood nematode produce extracellular proteases. | Paiva G, Proenca DN, Francisco R, Verissimo P, Santos SS, Fonseca L, Abrantes IM, Morais PV. | PLoS One | 10.1371/journal.pone.0079705 | 2013 | |
| Enzymology | Characterization of the fatty acid synthetase system of Curtobacterium pusillum. | Kawaguchi A, Uemura N, Okuda S. | J Biochem | 10.1093/oxfordjournals.jbchem.a135650 | 1986 | |
| Phylogeny | Cultivating the Bacterial Microbiota of Populus Roots. | Carper DL, Weston DJ, Barde A, Timm CM, Lu TY, Burdick LH, Jawdy SS, Klingeman DM, Robeson MS, Veach AM, Cregger MA, Kalluri UC, Schadt CW, Podar M, Doktycz MJ, Pelletier DA. | mSystems | 10.1128/msystems.01306-20 | 2021 | |
| Phylogeny | Alicyclobacillus spp.: New Insights on Ecology and Preserving Food Quality through New Approaches. | Ciuffreda E, Bevilacqua A, Sinigaglia M, Corbo MR. | Microorganisms | 10.3390/microorganisms3040625 | 2015 | |
| Distribution and diversity of olefins and olefin-biosynthesis genes in Gram-positive bacteria. | Surger M, Angelov A, Liebl W. | Biotechnol Biofuels | 10.1186/s13068-020-01706-y | 2020 | | |
| Phylogeny | Nucleic acid hybridization studies on Microbacterium, Curtobacterium, Agromyces and related taxa. | Dopfer H, Stackebrandt E, Fiedler F | J Gen Microbiol | 10.1099/00221287-128-8-1697 | 1982 | |
| Phylogeny | Curtobacterium ginsengisoli sp. nov., isolated from soil of a ginseng field. | Kim MK, Kim YJ, Kim HB, Kim SY, Yi TH, Yang DC | Int J Syst Evol Microbiol | 10.1099/ijs.0.65591-0 | 2008 | |
How to citeIf you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive7317.20251217.10
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain data
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