Methylovorus mays (DSM 16018, NCIMB 13992, VKM B-2221)

Name: Methylovorus mays (DSM 16018, NCIMB 13992, VKM B-2221)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7245

Type strain

Strain Designation BV, C, KUK

Culture col. no. DSM 16018 NCIMB 13992 VKM B-2221 BV C 1 more

NCBI tax ID(s) 184077

Special Collections DSMZ

History DSMZ <- Y. A. Trotsenko, Inst. Biochem. Physiol. Microorg., RAS, Pushchino, Russia; KUK <- N. V. Doronina

Links

Methylovorus mays BV is a bacterium that was isolated from phyllosphere of Zea mays L..

genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 100772

VKM B-2221,NCIMB 13992,DSM 16018

@ref 20215

Last LPSN update 2026-05-29 08:26:36

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Spirillales

Family Methylophilaceae

Genus Methylovorus

Species Methylovorus mays

Full scientific name Methylovorus mays Doronina et al. 2001

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	99.225

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
6143	MEDIUM K (DSMZ Medium 1122)		Medium recipe at MediaDive	Name: MEDIUM K (DSMZ Medium 1122) Composition: Agar 20.0 g/l KH2PO4 2.0 g/l (NH4)2SO4 2.0 g/l NaCl 0.5 g/l MgSO4 x 7 H2O 0.125 g/l FeSO4 x 7 H2O 0.002 g/l Methanol Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
6143	positive	growth	28

Physiology and metabolism

Spore formation

@ref	Spore formation	Confidence
125439		99.961

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Leaf (Phyllosphere)

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent
6143	phyllosphere of Zea mays L.	Zea mays	Moscow region	Russia	RUS	Europe

Taxonmaps

69479

Global distribution of 16S sequence AY486132 (>99% sequence identity) for Methylovorus from Microbeatlas

Aquatic Samples	3002
Soil Samples	3399
Animal Samples	792
Plant Samples	2670
Total Samples	9863

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6143	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM2053191v1 assembly for Methylovorus mays VKM B-2221	scaffold	GCA_020531915	184077.4	8063245465	184077	73.48

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
6143	Methylovorus mays strain C 16S ribosomal RNA gene, partial sequence	AY486132	1440		184077

GC content

@ref	GC-content (mol%)	Method
6143	57.2	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM2053191v1 assembly for Methylovorus mays VKM B-2221
NCBI: GCA_020531915

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate anaerobe	65.30	no
125439	gram_stain	BacteriaNetⓘ	negative	99.23	no
125439	motility	BacteriaNetⓘ	yes	80.71	no
125439	spore_formation	BacteriaNetⓘ	no	99.96	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Enhanced L-theanine production through semi-rational design of gamma-glutamylmethylamide synthetase from Methylovorus mays.	Fan C, Qi J, Cong Y, Zhang C.	Enzyme Microb Technol	10.1016/j.enzmictec.2024.110481	2024
Enzymology	Efficient synthesis of gamma-glutamyl compounds by co-expression of gamma-glutamylmethylamide synthetase and polyphosphate kinase in engineered Escherichia coli.	Pan X, Yu J, Du Q, Zeng S, Liu J, Jiao Q, Zhang H.	J Ind Microbiol Biotechnol	10.1007/s10295-020-02305-4	2020
Metabolism	Production of L-Theanine Using Escherichia coli Whole-Cell Overexpressing gamma-Glutamylmethylamide Synthetase with Bakers Yeast.	Yang SY, Han YH, Park YL, Park JY, No SY, Jeong D, Park S, Park HY, Kim W, Seo SO, Yang YH.	J Microbiol Biotechnol	10.4014/jmb.1910.10044	2020
	[Construction of recombinant strains co-expressing PPK and GMAS for the synthesis of L-theanine].	Li Y, Liu S, Zhu J.	Sheng Wu Gong Cheng Xue Bao	10.13345/j.cjb.160218	2016
Metabolism	[The obtainment and characteristics of Kalanchoe pinnata L. plants expressing the artificial gene of the cecropin P1 antimicrobial peptide].	Zakharchenko NS, Rukavtsova EB, Shevchuk TV, Furs OV, Pigoleva SV, Lebedeva AA, Chulina IA, Baidakova LK, Bur'yanov YI.	Prikl Biokhim Mikrobiol	10.1134/s0003683816040189	2016
	A high-throughput dual system to screen polyphosphate kinase mutants for efficient ATP regeneration in L-theanine biocatalysis.	Gao H, Li M, Wang Q, Liu T, Zhang X, Yang T, Xu M, Rao Z.	Biotechnol Biofuels Bioprod	10.1186/s13068-023-02361-9	2023
Metabolism	[Effect of plant-associated methanolic bacteria on methane and methanol concentration dynamics in atmosphere of pressurized chamber].	Berkovich IuA, Doronina NV, Fedorov DN, Mukhamedieva LN, Mikos KN, Krivobock NM, Smolianin VG, Smolianina SO, Shanturin NA.	Aviakosm Ekolog Med		2010
Metabolism	Specialized Metabolites from Methylotrophic Proteobacteria.	Puri AW.	Curr Issues Mol Biol	10.21775/cimb.033.211	2019
	Pathway engineering of Escherichia coli for one-step fermentative production of L-theanine from sugars and ethylamine.	Fan X, Zhang T, Ji Y, Li J, Long K, Yuan Y, Li Y, Xu Q, Chen N, Xie X.	Metab Eng Commun	10.1016/j.mec.2020.e00151	2020
Enzymology	Theanine production by coupled fermentation with energy transfer using gamma-glutamylmethylamide synthetase of Methylovorus mays No. 9.	Yamamoto S, Morihara Y, Wakayama M, Tachiki T.	Biosci Biotechnol Biochem	10.1271/bbb.70663	2008
Metabolism	Cloning and expression of Methylovorus mays No. 9 gene encoding gamma-glutamylmethylamide synthetase: an enzyme usable in theanine formation by coupling with the alcoholic fermentation system of baker's yeast.	Yamamoto S, Wakayama M, Tachiki T.	Biosci Biotechnol Biochem	10.1271/bbb.70462	2008
	[The influence of colonizing methylobacteria on morphogenesis and resistance of sugar beet and white cabbage plants to Erwinia carotovora].	Pigoleva SV, Zakharchenko NS, Pigolev AV, Trotsenko IuA, Bur'ianov IaI.	Prikl Biokhim Mikrobiol	10.1134/s0003683809060052	2009
Enzymology	Characterization of theanine-forming enzyme from Methylovorus mays no. 9 in respect to utilization of theanine production.	Yamamoto S, Wakayama M, Tachiki T.	Biosci Biotechnol Biochem	10.1271/bbb.60590	2007
	Structure and evolution of alanine/serine decarboxylases and the engineering of theanine production.	Wang H, Zhu B, Qiao S, Dong C, Wan X, Gong W, Zhang Z.	Elife	10.7554/elife.91046	2024
Phylogeny	Phylogenetic position and emended description of the genus Methylovorus.	Doronina NV, Ivanova EG, Trotsenko YA.	Int J Syst Evol Microbiol	10.1099/ijs.0.63111-0	2005
Enzymology	[Methylovorus mays--novel species of aerobic, obligatory methylotrophic bacteria associated with plants].	Doronina NV, Kudinova LV, Trotsenko IuA.	Mikrobiologiia	10.1007/bf02756815	2000
Metabolism	{gamma}-Glutamylmethylamide is an essential intermediate in the metabolism of methylamine by Methylocella silvestris.	Chen Y, Scanlan J, Song L, Crombie A, Rahman MT, Schafer H, Murrell JC.	Appl Environ Microbiol	10.1128/aem.00739-10	2010
	Fermentative N-Methylanthranilate Production by Engineered Corynebacterium glutamicum.	Walter T, Al Medani N, Burgardt A, Cankar K, Ferrer L, Kerbs A, Lee JH, Mindt M, Risse JM, Wendisch VF.	Microorganisms	10.3390/microorganisms8060866	2020
Metabolism	Carbaryl as a Carbon and Nitrogen Source: an Inducible Methylamine Metabolic Pathway at the Biochemical and Molecular Levels in Pseudomonas sp. Strain C5pp.	Kamini, Sharma R, Punekar NS, Phale PS.	Appl Environ Microbiol	10.1128/aem.01866-18	2018
Metabolism	Crystal structures of gamma-glutamylmethylamide synthetase provide insight into bacterial metabolism of oceanic monomethylamine.	Wang N, Chen XL, Gao C, Peng M, Wang P, Zhang N, Li F, Yang GP, Shen QT, Li S, Chen Y, Zhang YZ, Li CY.	J Biol Chem	10.1074/jbc.ra120.015952	2021
Cultivation	[Facultative and obligate aerobic methylobacteria synthesize cytokinins].	Ivanova EG, Doronina NV, Shepeliakovskaia AO, Laman AG, Brovko FA, Trotsenko IuA	Mikrobiologiia		2000

References

#6143	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16018
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methylovorus mays (DSM 16018, NCIMB 13992, VKM B-2221)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_020531915

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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