Name: Methylophilus luteus Mim
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7243

Type strain:

Species: Methylophilus luteus

Strain Designation: Mim

Culture col. no.: DSM 22949, CCUG 58412, VKM B-2548

Strain history: <- Y. A. Trotsenko, Inst. Biochem. Physiol. Microorg., RAS, Pushchino, Russia; Mim <- N. V. Doronina

NCBI tax ID(s): 640108 (species)

SI-ID 403749

DSM 22949, CCUG 58412

Section

Methylophilus luteus Mim is an aerobe, spore-forming, mesophilic bacterium that was isolated from phyllosphere of coltsfoot Tussilago farfara L..

Gram-negative
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Nitrosomonadales*
	Family	*Methylophilaceae*
	Genus	*Methylophilus*
	Species	**Methylophilus luteus**
	Full Scientific Name (LPSN)	Methylophilus luteus Gogleva et al. 2010

Information on morphological and physiological properties Morphology

[Ref.: #29722]	Gram stain	negative

[Ref.: #29722]	Cell length	1.9-2 µm

[Ref.: #29722]	Cell width	0.2-0.3 µm

[Ref.: #29722]	Cell shape	rod-shaped

[Ref.: #29722]	Motility	no

[Ref.: #29722]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16627]

Culture medium

MEDIUM K (DSMZ Medium 1122)

[Ref.: #16627]

Culture medium growth

[Ref.: #16627]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16627]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #16627]	growth	28 °C
[Ref.: #29722]	growth	24-26 °C
[Ref.: #29722]	optimum	24-26 °C

[Ref.: #16627]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #29722]			optimum	7.2-7.8
[Ref.: #29722]			growth	7.2-7.8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29722]

Oxygen tolerance

aerobe

[Ref.: #29722]

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29722]		NaCl		growth	0.05	%
[Ref.: #29722]		NaCl		optimum	0.05	%

[Ref.: #29722]

Observation

aggregates in chains

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29722]		glutamate ChEBI	+	carbon source

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #29722]		catalase	+	1.11.1.6
[Ref.: #29722]		cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16627]	Sample type/isolated from	phyllosphere of coltsfoot Tussilago farfara L.
[Ref.: #16627]	Host species	Tussilago farfara
[Ref.: #16627]	Geographic location (country and/or sea, region)	Moscow, Pushchino
[Ref.: #16627]	Country	Russia
[Ref.: #16627]	Country ISO 3 Code	RUS
[Ref.: #16627]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Leaf (Phyllosphere)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FJ872109 (>99% sequence identity) for Methylophilus from Microbeatlas

Aquatic Samples	478
Soil Samples	122
Animal Samples	350
Plant Samples	330
Total Samples	1280

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16627]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16627]	Methylophilus luteus strain Mim 16S ribosomal RNA gene, partial sequence	FJ872109	1418	ENA	640108 tax ID

[Ref.: #16627]	GC-content	54.5 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #29722]	GC-content	54.5 mol%

Availability in culture collections External links

[Ref.: #16627]

Culture collection no.

DSM 22949, CCUG 58412, VKM B-2548

[Ref.: #76695]

SI-ID 403749

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methylophilus flavus sp. nov. and Methylophilus luteus sp. nov., aerobic, methylotrophic bacteria associated with plants.	Gogleva AA, Kaparullina EN, Doronina NV, Trotsenko YA	Int J Syst Evol Microbiol	10.1099/ijs.0.019455-0	2009	*

References

#16627

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22949

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29722

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26105 (see below)

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#76695

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403749.1 )

#26105

IJSEM 2623 2010 ( DOI 10.1099/ijs.0.019455-0 , PubMed 20023062 )

* These data were automatically processed and therefore are not curated

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