Methylophilus flavus (DSM 23073, Ship, VKM B-2547)

Name: Methylophilus flavus (DSM 23073, Ship, VKM B-2547)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7242

Type strain

Strain Designation Ship

Culture col. no. DSM 23073 Ship VKM B-2547

NCBI tax ID(s) 640084

Special Collections DSMZ Literature strains

History DSMZ <- Y. A. Trotsenko, Inst. Biochem. Physiol. Microorg., RAS, Pushchino, Russia; Ship <- N. V. Doronina

Links

Methylophilus flavus Ship is an aerobe, spore-forming, Gram-negative bacterium that was isolated from phyllosphere of dog rose Rosa cinnamomea L..

spore-forming Gram-negative rod-shaped aerobe 16S sequence Bacteria

Name and taxonomic classification

SI-ID 403749

VKM B-2548,VKM B-2547,DSM 22949,CCUG 58412,CCUG 58411T

@ref 20215

Last LPSN update 2026-05-29 08:26:36

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Spirillales

Family Methylophilaceae

Genus Methylophilus

Species Methylophilus flavus

Full scientific name Methylophilus flavus Gogleva et al. 2010

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
29722	negative	1.9-2 µm	0.2-0.3 µm	rod-shaped

Pigmentation

29722

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
16691	MEDIUM K (DSMZ Medium 1122)		Medium recipe at MediaDive	Name: MEDIUM K (DSMZ Medium 1122) Composition: Agar 20.0 g/l KH2PO4 2.0 g/l (NH4)2SO4 2.0 g/l NaCl 0.5 g/l MgSO4 x 7 H2O 0.125 g/l FeSO4 x 7 H2O 0.002 g/l Methanol Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
16691	positive	growth	28
29722	positive	growth	19-24
29722	positive	optimum	19-24

Culture pH

@ref	Ability	Type	PH
29722	positive	optimum	7.2-7.8
29722	positive	growth	7.2-7.8

Physiology and metabolism

Oxygen tolerance

29722

Oxygen toleranceaerobe

Spore formation

29722

Spore formationyes

Halophily

@ref	Salt	Growth	Tested relation	Concentration
29722	NaCl	positive	growth	0.05 %
29722	NaCl	positive	optimum	0.05 %

Observation

29722

Observationaggregates in chains

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
29722	29987 ChEBI	glutamate	+	carbon source

Enzymes

@ref	Value	Activity	Ec
29722	catalase	+	1.11.1.6
29722	cytochrome oxidase	+	1.9.3.1

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Shrub (Scrub)
#Host Body-Site	#Plant	#Leaf (Phyllosphere)

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent
16691	phyllosphere of dog rose Rosa cinnamomea L.	Rosa cinnamomea	Moscow, Pushchino	Russia	RUS	Europe

Taxonmaps

69479

Global distribution of 16S sequence FJ872108 (>99% sequence identity) for Methylophilus from Microbeatlas

Aquatic Samples	478
Soil Samples	122
Animal Samples	350
Plant Samples	330
Total Samples	1280

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
16691	1	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
16691	Methylophilus flavus strain Ship 16S ribosomal RNA gene, partial sequence	FJ872108	1430		640084

GC content

@ref	GC-content (mol%)	Method
16691	50.7	thermal denaturation, midpoint method (Tm)
29722	50.7

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Prerequisites for amplicon pyrosequencing of microbial methanol utilizers in the environment.	Kolb S, Stacheter A.	Front Microbiol	10.3389/fmicb.2013.00268	2013
	Bioprospects of Endophytic Bacteria in Plant Growth Promotion and Ag-Nanoparticle Biosynthesis.	Singh M, Qureshi KA, Jaremko M, Rajput M, Singh SK, Kaushalendra, Pandey KD, Ferreira LFR, Kumar A.	Plants (Basel)	10.3390/plants11141787	2022
Metabolism	[Enhanced Resistance of Pea Plants to Oxidative: Stress Caused by Paraquat during Colonization by Aerobic Methylobacteria].	Agafonova NV, Doronina NY, Trotsenko YA.	Prikl Biokhim Mikrobiol	10.1134/s0003683816020022	2016
Phylogeny	Methylophilus flavus sp. nov. and Methylophilus luteus sp. nov., aerobic, methylotrophic bacteria associated with plants.	Gogleva AA, Kaparullina EN, Doronina NV, Trotsenko YA	Int J Syst Evol Microbiol	10.1099/ijs.0.019455-0	2009

References

#16691	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23073
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#26105	IJSEM 2623 2010 ( DOI 10.1099/ijs.0.019455-0 , PubMed 20023062 )
#29722	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26105
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methylophilus flavus (DSM 23073, Ship, VKM B-2547)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

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