Name: Methylophilus methylotrophus DSM 5691
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7239

Type strain:

Species: Methylophilus methylotrophus

Culture col. no.: DSM 5691, ATCC 53528, CCUG 58724, DSM 46235, NCIMB 10515, LMG 6787, BCRC 15784, KCTC 1759, AS 1

Strain history: <- NCIMB <- T. R. Owen, ICI <- D. Byrom, ICI; AS1

NCBI tax ID(s): 1122236 (strain), 17 (species)

SI-ID 3574

LMG 6787, IMET 10786, LMG 6081, NCIB 10515, NCIMB 10515, ATCC 53528, DSM 46235, DSM 5691, VKM B-1623, KCTC 1759, BCRC 15784, CCRC 15784, CCUG 58724

Other strains from Methylophilus methylotrophus

M. methylotrophus DSM 6330, ATCC 31226
M. methylotrophus

Section

Methylophilus methylotrophus DSM 5691 is an aerobe, mesophilic, motile bacterium that was isolated from activated sludge.

motile
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Nitrosomonadales*
	Family	*Methylophilaceae*
	Genus	*Methylophilus*
	Species	**Methylophilus methylotrophus**
	Full Scientific Name (LPSN)	Methylophilus methylotrophus Jenkins et al. 1987

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.999

[Ref.: #69480]	Motility	yes Confidence in %96.21

[Ref.: #61745]

Incubation period

4 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2288]

Culture medium

COLBY AND ZATHMAN MEDIUM (DSMZ Medium 606)

[Ref.: #2288]

Culture medium growth

[Ref.: #2288]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2288]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #2288]			growth	30 °C
[Ref.: #61745]			growth	30 °C

[Ref.: #2288]	Temperature range	mesophilic
[Ref.: #61745]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #61745]

Oxygen tolerance

aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %100

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #2288]	Sample type/isolated from	activated sludge
[Ref.: #2288]	Continent	Europe

[Ref.: #61745]	Sample type/isolated from	Activated sludge
[Ref.: #61745]	Country	United Kingdom
[Ref.: #61745]	Country ISO 3 Code	GBR
[Ref.: #61745]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered

#Waste

#Activated sludge

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB193724 (>99% sequence identity) for Methylophilus from Microbeatlas

Aquatic Samples	1592
Soil Samples	376
Animal Samples	2109
Plant Samples	383
Total Samples	4460

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2288]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Methylophilus methylotrophus (ATCC 53528) 16S ribosomal RNA	L15475	1486	ENA	17 tax ID	*
[Ref.: #20218]	Methylophilus methylotrophus gene for 16S rRNA	AB193724	1498	ENA	17 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methylophilus methylotrophus DSM 46235 = ATCC 53528	GCA_000378225	scaffold	GenBank	1122236 tax ID	*
[Ref.: #66792]	Methylophilus methylotrophus DSM 46235 = ATCC 53528	1122236.3	wgs	PATRIC	1122236 tax ID	*
[Ref.: #66792]	Methylophilus methylotrophus ATCC 53528	2518645588	draft	JGI IMG/M	1122236 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Methylophilus methylotrophus DSM 46235 = ATCC 53528 ATCC 53528 NCBI: GCA_000378225.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methylophilus methylotrophus DSM 46235 = ATCC 53528 ATCC 53528 NCBI: GCA_000378225.1
[Ref.: #69480]	motile	yes	89.41	no
[Ref.: #69480]	flagellated	yes	62.574	no
[Ref.: #69480]	gram-positive	no	99.019	no
[Ref.: #69480]	anaerobic	no	98.72	yes
[Ref.: #69480]	aerobic	yes	84.03	yes
[Ref.: #69480]	halophile	no	94.128	no
[Ref.: #69480]	spore-forming	no	95.252	no
[Ref.: #69480]	thermophile	no	97.281	yes
[Ref.: #69480]	glucose-util	yes	60.025	no
[Ref.: #69480]	glucose-ferment	no	91.393	no

Availability in culture collections External links

[Ref.: #2288]

Culture collection no.

DSM 5691, ATCC 53528, CCUG 58724, DSM 46235, NCIMB 10515, LMG 6787, BCRC 15784, KCTC 1759, AS 1

[Ref.: #76691]

SI-ID 3574

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Genetics	Aromatic amino acid auxotrophs constructed by recombinant marker exchange in Methylophilus methylotrophus AS1 cells expressing the aroP-encoded transporter of Escherichia coli.	Yomantas YA, Tokmakova IL, Gorshkova NV, Abalakina EG, Kazakova SM, Gak ER, Mashko SV	Appl Environ Microbiol	10.1128/AEM.02217-09	2009	*
Metabolism	Disruption of metF increased L-lysine production by Methylophilus methylotrophus from methanol.	Ishikawa K, Asahara T, Gunji Y, Yasueda H, Asano K	Biosci Biotechnol Biochem	10.1271/bbb.70818	2008	*
Metabolism	Enhancement of L-lysine production in methylotroph Methylophilus methylotrophus by introducing a mutant LysE exporter.	Gunji Y, Yasueda H	J Biotechnol	10.1016/j.jbiotec.2006.06.003	2006	*
Enzymology	L-Lysine biosynthetic pathway of Methylophilus methylotrophus and construction of an L-lysine producer.	Tsujimoto N, Gunji Y, Ogawa-Miyata Y, Shimaoka M, Yasueda H	J Biotechnol	10.1016/j.jbiotec.2005.12.026	2006	*
Metabolism	Characterization of the L-lysine biosynthetic pathway in the obligate methylotroph Methylophilus methylotrophus.	Gunji Y, Tsujimoto N, Shimaoka M, Ogawa-Miyata Y, Sugimoto S, Yasueda H	Biosci Biotechnol Biochem	10.1271/bbb.68.1449	2004	*
Enzymology	Identification of methanol-regulated promoter sequences from the facultative methylotrophic bacterium Methylobacterium organophilum XX.	Xu HH, Viebahn M, Hanson RS	J Gen Microbiol	10.1099/00221287-139-4-743	1993	*
Phylogeny	16S ribosomal RNA sequence analysis for determination of phylogenetic relationship among methylotrophs.	Tsuji K, Tsien HC, Hanson RS, DePalma SR, Scholtz R, LaRoche S	J Gen Microbiol	10.1099/00221287-136-1-1	1990	*
Phylogeny	Genetic mapping in Methylophilus methylotrophus AS1.	Moore AT, Nayudu M, Holloway BW	J Gen Microbiol	10.1099/00221287-129-3-785	1983	*
	Creating auxotrophic mutants in Methylophilus methylotrophus AS1 by combining electroporation and chemical mutagenesis.	Kim CS, Wood TK	Appl Microbiol Biotechnol	10.1007/s002530051023	1997	*

References

#2288

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 5691

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#61745 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 58724

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76691

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID3574.1 )

* These data were automatically processed and therefore are not curated

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Methylophilus methylotrophus DSM 46235 = ATCC 53528 ATCC 53528

Methylophilus methylotrophus DSM 46235 = ATCC 53528 ATCC 53528

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