Methylosoma difficile (DSM 18750, JCM 14076, LC 2)

Name: Methylosoma difficile (DSM 18750, JCM 14076, LC 2)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7212

Type strain

Strain Designation LC 2

Culture col. no. DSM 18750 JCM 14076 LC 2

NCBI tax ID(s) 392711

Special Collections DSMZ JCM Literature strains

Links

Methylosoma difficile LC 2 is a microaerophile, psychrophilic, Gram-negative prokaryote that was isolated from littoral sediment.

Gram-negative coccus-shaped microaerophile psychrophilic 16S sequence

Name and taxonomic classification

SI-ID 308620

DSM 18750,JCM 14076

@ref 20215

Last LPSN update 2025-12-16 09:48:31

Domain Pseudomonadati

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Methylococcales

Family Methylococcaceae

Genus Methylosoma

Species Methylosoma difficile

Full scientific name Methylosoma difficile Rahalkar et al. 2007

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
31873	negative	1.75 µm	1 µm	coccus-shaped

Pigmentation

31873

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
7683	LC 2 (DSMZ Medium 1132)		Medium recipe at MediaDive	Name: LC 2 (DSMZ Medium 1132) Composition: MgSO4 x 7 H2O 1.0 g/l HEPES buffer 0.4766 g/l Methanol 0.2376 g/l CaCl2 x 2 H2O 0.2 g/l Na2HPO4 0.072 g/l KNO3 0.05 g/l KH2PO4 0.028 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Fe(III)NH4-EDTA Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
7683	positive	growth	20	psychrophilic
31873	positive	growth	10-30
31873	positive	optimum	25	mesophilic
67770	positive	growth	20	psychrophilic

Culture pH

@ref	Ability	Type	PH
31873	positive	optimum	7
31873	positive	growth	05-09

Physiology and metabolism

Oxygen tolerance

31873

Oxygen tolerancemicroaerophile

Spore formation

31873

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
31873	NaCl	positive	growth	0.5-2.0 %
31873	NaCl	positive	optimum	1.25 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
31873	22653 ChEBI	asparagine	+	carbon source
31873	35391 ChEBI	aspartate	+	carbon source
31873	29987 ChEBI	glutamate	+	carbon source

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Sediment

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
7683	littoral sediment	Lake Constance, littoral zone	Germany	DEU	Europe
67770	Littoral sediment of Lake Constance		Germany	DEU	Europe

Taxonmaps

69479

Global distribution of 16S sequence DQ119050 (>99% sequence identity) for Methylosoma difficile subclade from Microbeatlas

Aquatic Samples	583
Soil Samples	65
Animal Samples	28
Plant Samples	11
Total Samples	687

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
7683	1	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
7683	Methylosoma difficile strain LC 2 16S ribosomal RNA gene, partial sequence	DQ119050	1491		392711

GC content

@ref	GC-content (mol%)	Method
7683	49.9	high performance liquid chromatography (HPLC)
31873	49.9

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Diversity and Habitat Preferences of Cultivated and Uncultivated Aerobic Methanotrophic Bacteria Evaluated Based on pmoA as Molecular Marker.	Knief C.	Front Microbiol	10.3389/fmicb.2015.01346	2015
Phylogeny	Methylovulum miyakonense gen. nov., sp. nov., a type I methanotroph isolated from forest soil.	Iguchi H, Yurimoto H, Sakai Y	Int J Syst Evol Microbiol	10.1099/ijs.0.019604-0	2010
Phylogeny	Methylosoma difficile gen. nov., sp. nov., a novel methanotroph enriched by gradient cultivation from littoral sediment of Lake Constance.	Rahalkar M, Bussmann I, Schink B	Int J Syst Evol Microbiol	10.1099/ijs.0.64574-0	2007

References

#7683	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18750
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#31873	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28133 (see below)
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methylosoma difficile (DSM 18750, JCM 14076, LC 2)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

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