Name: Methylosoma difficile LC 2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7212

Type strain:

Species: Methylosoma difficile

Strain Designation: LC 2

Culture col. no.: DSM 18750, JCM 14076

Strain history: M. Rahalkar LC2.

NCBI tax ID(s): 392711 (species)

SI-ID 308620

DSM 18750, JCM 14076

Section

Methylosoma difficile LC 2 is a microaerophile, psychrophilic, Gram-negative bacterium that was isolated from littoral sediment.

coccus-shaped
Gram-negative
microaerophile
psychrophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Methylococcales*
	Family	*Methylococcaceae*
	Genus	*Methylosoma*
	Species	**Methylosoma difficile**
	Full Scientific Name (LPSN)	Methylosoma difficile Rahalkar et al. 2007

Information on morphological and physiological properties Morphology

[Ref.: #31873]	Gram stain	negative

[Ref.: #31873]	Cell length	1.75 µm

[Ref.: #31873]	Cell width	1 µm

[Ref.: #31873]	Cell shape	coccus-shaped

[Ref.: #31873]	Motility	no

[Ref.: #31873]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7683]

Culture medium

LC 2 (DSMZ Medium 1132)

[Ref.: #7683]

Culture medium growth

[Ref.: #7683]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7683]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #7683]	growth	20 °C
[Ref.: #31873]	growth	10-30 °C
[Ref.: #31873]	optimum	25 °C
[Ref.: #67770]	growth	20 °C

[Ref.: #7683]	Temperature range	psychrophilic
[Ref.: #31873]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	psychrophilic

	pH	Kind of pH		pH
[Ref.: #31873]			optimum	7
[Ref.: #31873]			growth	05-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31873]

Oxygen tolerance

microaerophile

[Ref.: #31873]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31873]		NaCl		growth	0.5-2.0	%
[Ref.: #31873]		NaCl		optimum	1.25	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31873]	asparagine ChEBI	+	carbon source
[Ref.: #31873]	aspartate ChEBI	+	carbon source
[Ref.: #31873]	glutamate ChEBI	+	carbon source

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7683]	Sample type/isolated from	littoral sediment
[Ref.: #7683]	Geographic location (country and/or sea, region)	Lake Constance, littoral zone
[Ref.: #7683]	Country	Germany
[Ref.: #7683]	Country ISO 3 Code	DEU
[Ref.: #7683]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Littoral sediment of Lake Constance
[Ref.: #67770]	Country	Germany
[Ref.: #67770]	Country ISO 3 Code	DEU
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Sediment

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence DQ119050 (>99% sequence identity) for Methylosoma difficile subclade from Microbeatlas

Aquatic Samples	583
Soil Samples	65
Animal Samples	28
Plant Samples	11
Total Samples	687

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7683]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7683]	Methylosoma difficile strain LC 2 16S ribosomal RNA gene, partial sequence	DQ119050	1491	ENA	392711 tax ID

[Ref.: #7683]	GC-content	49.9 mol%	high performance liquid chromatography (HPLC)
[Ref.: #31873]	GC-content	49.9 mol%

Availability in culture collections External links

[Ref.: #7683]

Culture collection no.

DSM 18750, JCM 14076

[Ref.: #76665]

SI-ID 308620

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methylovulum miyakonense gen. nov., sp. nov., a type I methanotroph isolated from forest soil.	Iguchi H, Yurimoto H, Sakai Y	Int J Syst Evol Microbiol	10.1099/ijs.0.019604-0	2010	*
Phylogeny	Methylosoma difficile gen. nov., sp. nov., a novel methanotroph enriched by gradient cultivation from littoral sediment of Lake Constance.	Rahalkar M, Bussmann I, Schink B	Int J Syst Evol Microbiol	10.1099/ijs.0.64574-0	2007	*

References

#7683

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18750

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31873

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28133 (see below)

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#76665

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID308620.1 )

#28133

IJSEM 1073 2007 ( DOI 10.1099/ijs.0.64574-0 , PubMed 17473262 )

* These data were automatically processed and therefore are not curated

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