Name: Roseomonas aquatica TR53
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 72

Type strain:

Species: Roseomonas aquatica

Strain Designation: TR53

Culture col. no.: DSM 19438, CECT 7131, JCM 13556, CCM 7358, NCIMB 14503, CIP 109393

Strain history: CIP <- 2006, CECT <- A. Ventosa: strain TR53

NCBI tax ID(s): 373043 (species)

SI-ID 297309

CECT 7131, JCM 13556, CCM 7358, CIP 109393, DSM 19438, KCTC 22105

Section

Roseomonas aquatica TR53 is an obligate aerobe, mesophilic, Gram-negative bacterium that forms irregular colonies and was isolated from drinking water distribution system of Seville.

colony-forming
Gram-negative
mesophilic
obligate aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodospirillales*
	Family	*Acetobacteraceae*
	Genus	*Roseomonas*
	Species	**Roseomonas aquatica**
	Full Scientific Name (LPSN)	Roseomonas aquatica Gallego et al. 2006

Synonym

Paeniroseomonas aquatica

Information on morphological and physiological properties Morphology

[Ref.: #22959]	Gram stain	negative
[Ref.: #116433]	Gram stain	negative

[Ref.: #22959]	Cell length	1.2-2.0 µm

[Ref.: #22959]	Cell width	1.0 µm

[Ref.: #22959]	Motility	no
[Ref.: #116433]	Motility	no

[Ref.: #116433]	Cell shape	oval-shaped

[Ref.: #22959]	Colony size	0.5-1.0 mm
[Ref.: #22959]	Colony color	pale-pink-pigmented
[Ref.: #22959]	Colony shape	irregular
[Ref.: #22959]	Incubation period	4 days
[Ref.: #22959]	Cultivation medium used	R2A Agar

[Ref.: #22959]	Colony size	<0.5 mm
[Ref.: #22959]	Cultivation medium used	nutrient agar medium

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8017]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #8017]

Culture medium growth

[Ref.: #8017]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8017]

Culture medium composition

expand / minimize

[Ref.: #22959]

Culture medium

nutrient agar medium

[Ref.: #22959]

Culture medium growth

[Ref.: #22959]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #22959]

Culture medium growth

[Ref.: #37542]

Culture medium

MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromati

[Ref.: #37542]

Culture medium growth

[Ref.: #37542]

Culture medium composition

expand / minimize

[Ref.: #116433]

Culture medium

CIP Medium 566

[Ref.: #116433]

Culture medium growth

[Ref.: #116433]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #8017]	growth	28 °C
[Ref.: #22959]	growth	15.0-35.0 °C
[Ref.: #22959]	optimum	25.0-28.0 °C
[Ref.: #37542]	growth	30 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #8017]	Temperature range	mesophilic
[Ref.: #22959]	Temperature range	mesophilic
[Ref.: #37542]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #22959]			optimum	7.0
[Ref.: #22959]			growth	5.0-9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #22959]

Oxygen tolerance

obligate aerobe

[Ref.: #22959]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #22959]		NaCl	no	growth	>2.0	%

[Ref.: #67770]

Observation

quinones: Q-10

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #22959]	2-dehydro-D-gluconate ChEBI	-	carbon source
[Ref.: #22959]	5-dehydro-D-gluconate ChEBI	-	carbon source
[Ref.: #22959]	adipate ChEBI	-	carbon source
[Ref.: #22959]	amygdalin ChEBI	-	carbon source
[Ref.: #22959]	arbutin ChEBI	-	carbon source
[Ref.: #22959]	casein	-	hydrolysis
[Ref.: #22959]	cellobiose ChEBI	-	carbon source
[Ref.: #22959]	citric acid ChEBI	-	carbon source
[Ref.: #22959]	D-arabinose ChEBI	-	carbon source
[Ref.: #22959]	D-arabitol ChEBI	-	carbon source
[Ref.: #22959]	D-fructose ChEBI	-	builds acid from
[Ref.: #22959]	D-fructose ChEBI	-	carbon source
[Ref.: #22959]	D-fucose ChEBI	-	carbon source
[Ref.: #22959]	D-galactose ChEBI	-	builds acid from
[Ref.: #22959]	D-galactose ChEBI	-	carbon source
[Ref.: #22959]	D-glucose ChEBI	-	builds acid from
[Ref.: #22959]	D-glucose ChEBI	-	carbon source
[Ref.: #22959]	D-lyxose ChEBI	-	carbon source
[Ref.: #22959]	D-mannitol ChEBI	-	builds acid from
[Ref.: #22959]	D-mannitol ChEBI	-	carbon source
[Ref.: #22959]	D-mannose ChEBI	-	builds acid from
[Ref.: #22959]	D-mannose ChEBI	-	carbon source
[Ref.: #22959]	D-ribose ChEBI	-	carbon source
[Ref.: #22959]	D-sorbitol ChEBI	-	carbon source
[Ref.: #22959]	D-tagatose ChEBI	-	carbon source
[Ref.: #22959]	D-trehalose ChEBI	-	builds acid from
[Ref.: #22959]	D-trehalose ChEBI	-	carbon source
[Ref.: #22959]	D-xylose ChEBI	-	builds acid from
[Ref.: #22959]	D-xylose ChEBI	-	carbon source
[Ref.: #22959]	decanoate ChEBI	-	carbon source
[Ref.: #22959]	dna ChEBI	-	hydrolysis
[Ref.: #22959]	erythritol ChEBI	-	carbon source
[Ref.: #22959]	esculin ChEBI	-	carbon source
[Ref.: #22959]	galactitol ChEBI	-	carbon source
[Ref.: #22959]	gelatin ChEBI	-	hydrolysis
[Ref.: #22959]	gentiobiose ChEBI	-	carbon source
[Ref.: #22959]	glucose ChEBI	-	fermentation
[Ref.: #22959]	glycerol ChEBI	-	builds acid from
[Ref.: #22959]	glycerol ChEBI	-	carbon source
[Ref.: #22959]	glycogen ChEBI	-	carbon source
[Ref.: #22959]	inulin ChEBI	-	carbon source
[Ref.: #22959]	L-arabinose ChEBI	-	carbon source
[Ref.: #22959]	L-arabitol ChEBI	-	carbon source
[Ref.: #22959]	L-fucose ChEBI	-	carbon source
[Ref.: #22959]	L-rhamnose ChEBI	-	carbon source
[Ref.: #22959]	L-sorbose ChEBI	-	carbon source
[Ref.: #22959]	L-xylose ChEBI	-	carbon source
[Ref.: #22959]	lactose ChEBI	-	builds acid from
[Ref.: #22959]	lactose ChEBI	-	carbon source
[Ref.: #22959]	malate ChEBI	-	carbon source
[Ref.: #22959]	maltose ChEBI	-	builds acid from
[Ref.: #22959]	maltose ChEBI	-	carbon source
[Ref.: #22959]	melezitose ChEBI	-	carbon source
[Ref.: #22959]	melibiose ChEBI	-	carbon source
[Ref.: #22959]	methyl alpha-D-glucopyranoside ChEBI	-	carbon source
[Ref.: #22959]	methyl alpha-D-mannoside ChEBI	-	carbon source
[Ref.: #22959]	methyl beta-D-xylopyranoside ChEBI	-	carbon source
[Ref.: #22959]	myo-inositol ChEBI	-	carbon source
[Ref.: #22959]	N-acetylglucosamine ChEBI	-	carbon source
[Ref.: #22959]	nitrate ChEBI	+	reduction
[Ref.: #116433]	nitrate ChEBI	+	reduction
[Ref.: #116433]	nitrite ChEBI	-	reduction
[Ref.: #22959]	phenylacetate ChEBI	-	carbon source
[Ref.: #22959]	potassium gluconate ChEBI	-	carbon source
[Ref.: #22959]	raffinose ChEBI	-	carbon source
[Ref.: #22959]	ribitol ChEBI	-	carbon source
[Ref.: #22959]	salicin ChEBI	-	carbon source
[Ref.: #22959]	sodium citrate ChEBI	-	carbon source
[Ref.: #22959]	starch ChEBI	-	carbon source
[Ref.: #22959]	starch ChEBI	-	hydrolysis
[Ref.: #22959]	sucrose ChEBI	-	carbon source
[Ref.: #22959]	tryptophan ChEBI	-	energy source
[Ref.: #22959]	turanose ChEBI	-	carbon source
[Ref.: #22959]	tween 80 ChEBI	-	hydrolysis
[Ref.: #22959]	xylitol ChEBI	-	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic resistance	Concentration
[Ref.: #22959]	bacitracin ChEBI	yes	10	Unit
[Ref.: #22959]	cephalothin ChEBI	yes	30	µg (disc)
[Ref.: #22959]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #22959]	erythromycin ChEBI	yes	15	µg (disc)
[Ref.: #22959]	kanamycin ChEBI	yes	30	µg (disc)
[Ref.: #22959]	nalidixic acid ChEBI	yes	30	µg (disc)
[Ref.: #22959]	neomycin ChEBI	yes	10	µg (disc)
[Ref.: #22959]	novobiocin ChEBI	yes	30	µg (disc)
[Ref.: #22959]	penicillin ChEBI	yes	10	Unit
[Ref.: #22959]	rifampicin ChEBI	yes	30	µg (disc)
[Ref.: #22959]	streptomycin ChEBI	yes	10	µg (disc)
[Ref.: #22959]	tetracycline ChEBI	yes	30	µg (disc)
[Ref.: #22959]	vancomycin ChEBI	yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #22959]	acetoin ChEBI	no
[Ref.: #22959]	hydrogen sulfide ChEBI	no
[Ref.: #22959]	indole ChEBI	no
[Ref.: #116433]	indole ChEBI	no

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test	Citrate test
[Ref.: #22959]	acetoin ChEBI	-
[Ref.: #22959]	citrate ChEBI				-
[Ref.: #22959]	glucose ChEBI		-
[Ref.: #22959]	indole ChEBI			-

	Enzyme	Enzyme activity	EC number
[Ref.: #22959]	acid phosphatase	+	3.1.3.2
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #22959]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #22959]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #22959]	alpha-fucosidase	-	3.2.1.51
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #22959]	alpha-galactosidase	-	3.2.1.22
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #22959]	alpha-glucosidase	-	3.2.1.20
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #22959]	alpha-mannosidase	-	3.2.1.24
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #22959]	arginine dihydrolase	-	3.5.3.6
[Ref.: #22959]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #22959]	beta-glucosidase	-	3.2.1.21
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #22959]	beta-glucuronidase	-	3.2.1.31
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #22959]	catalase	+	1.11.1.6
[Ref.: #116433]	catalase	+	1.11.1.6
[Ref.: #22959]	cystine arylamidase	-	3.4.11.3
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #22959]	cytochrome oxidase	-	1.9.3.1
[Ref.: #22959]	esterase (C 4)	+
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #22959]	esterase lipase (C 8)	+
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #22959]	leucine arylamidase	+	3.4.11.1
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #22959]	lipase (C 14)	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #22959]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #22959]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #116433]	oxidase	-
[Ref.: #22959]	trypsin	-	3.4.21.4
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #22959]	tryptophan deaminase	-	4.1.99.1
[Ref.: #22959]	urease	+	3.5.1.5
[Ref.: #116433]	urease	+	3.5.1.5
[Ref.: #22959]	valine arylamidase	-
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116433]	11836	-	+	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8017]	Sample type/isolated from	drinking water distribution system of Seville
[Ref.: #8017]	Country	Spain
[Ref.: #8017]	Country ISO 3 Code	ESP
[Ref.: #8017]	Continent	Europe

[Ref.: #22959]	Sample type/isolated from	potable water
[Ref.: #22959]	Geographic location (country and/or sea, region)	Seville
[Ref.: #22959]	Country	Spain
[Ref.: #22959]	Country ISO 3 Code	ESP
[Ref.: #22959]	Continent	Europe
[Ref.: #22959]	Geographic location	37.3925°/-5.9925°
[Ref.: #22959]	Enrichment culture	plate count agar and R2A
[Ref.: #22959]	Enrichment culture duration	7 days
[Ref.: #22959]	Enrichment culture temperature	28.0 ̊C

[Ref.: #67770]	Sample type/isolated from	Drinking water distribution system of Seville
[Ref.: #67770]	Country	Spain
[Ref.: #67770]	Country ISO 3 Code	ESP
[Ref.: #67770]	Continent	Europe

[Ref.: #116433]	Sample type/isolated from	Environment, Drinking water
[Ref.: #116433]	Geographic location (country and/or sea, region)	Sevilla
[Ref.: #116433]	Country	Spain
[Ref.: #116433]	Country ISO 3 Code	ESP
[Ref.: #116433]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Freshwater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AM231587 (>99% sequence identity) for Roseomonas aquatica subclade from Microbeatlas

Aquatic Samples	761
Soil Samples	1130
Animal Samples	1125
Plant Samples	849
Total Samples	3865

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8017]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116433]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8017]	Roseomonas aquatica partial 16S rRNA gene, type strain TR53T	AM231587	1387	ENA	373043 tax ID

[Ref.: #8017]

GC-content

68.6 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #8017]

Culture collection no.

DSM 19438, CECT 7131, JCM 13556, CCM 7358, NCIMB 14503, CIP 109393

[Ref.: #69756]

SI-ID 297309

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Roseomonas aquatica sp. nov., isolated from drinking water.	Gallego V, Sanchez-Porro C, Garcia MT, Ventosa A	Int J Syst Evol Microbiol	10.1099/ijs.0.64379-0	2006	*

References

#8017

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19438

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#22959

Virginia Gallego,Cristina Sánchez-Porro,Maria Teresa García,Antonio Ventosa: Roseomonas aquatica sp. nov., isolated from drinking water. IJSEM 56: 2291 - 2295 2006 ( DOI 10.1099/ijs.0.64379-0 , PubMed 17012549 )

#37542 ; Curators of the CIP;
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69756

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID297309.1 )

#116433 Collection of Institut Pasteur ; Curators of the CIP; CIP 109393
* These data were automatically processed and therefore are not curated

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