Name: Methylobacterium cerastii C15
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 7197

Type strain:

Species: Methylobacterium cerastii

Strain Designation: C15, C20oh1-15

Culture col. no.: DSM 23679, CCM 7788, CCUG 60040

Strain history: <- P. Kämpfer, Justus-Liebig-Universität; C20oh1-15 <- S.A. Wellner

NCBI tax ID(s): 932741 (species)

SI-ID 379629

CCM 7788, CCUG 60040, DSM 23679, LMG 28249, NCIMB 14837

Special Collections

Other strains from Methylobacterium cerastii

Section

Methylobacterium cerastii C15 is an aerobe, Gram-negative, rod-shaped bacterium that was isolated from leaf surface of Cerastium holosteoides.

Gram-negative
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Methylobacteriaceae*
	Genus	*Methylobacterium*
	Species	**Methylobacterium cerastii**
	Full Scientific Name (LPSN)	Methylobacterium cerastii Wellner et al. 2012

Information on morphological and physiological properties Morphology

[Ref.: #30207]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %97.802

[Ref.: #30207]	Cell length	1.6-5.4 µm

[Ref.: #30207]	Cell width	0.8-1.1 µm

[Ref.: #30207]	Cell shape	rod-shaped

[Ref.: #30207]	Motility	no

[Ref.: #62192]

Incubation period

4 days

[Ref.: #30207]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #17341]

Culture medium

METHYLOBACTERIUM MEDIUM (DSMZ Medium 125)

[Ref.: #17341]

Culture medium growth

[Ref.: #17341]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17341]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #17341]	growth	28 °C
[Ref.: #30207]	growth	10-28 °C
[Ref.: #30207]	optimum	20 °C
[Ref.: #62192]	growth	25 °C

	pH	Kind of pH		pH
[Ref.: #30207]			optimum	05-06
[Ref.: #30207]			growth	04-06

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30207]	Oxygen tolerance	aerobe
[Ref.: #62192]	Oxygen tolerance	aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30207]		NaCl		growth	0.50	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30207]	glutamate ChEBI	+	carbon source
[Ref.: #30207]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #30207]	putrescine ChEBI	+	carbon source

	Enzyme	Enzyme activity	EC number
[Ref.: #30207]	catalase	+	1.11.1.6
[Ref.: #30207]	cytochrome oxidase	+	1.9.3.1
[Ref.: #30207]	urease	+	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17341]	Sample type/isolated from	leaf surface of Cerastium holosteoides
[Ref.: #17341]	Host species	Cerastium holosteoides
[Ref.: #17341]	Geographic location (country and/or sea, region)	Thüringen, exploratory Hainich-Dün
[Ref.: #17341]	Country	Germany
[Ref.: #17341]	Country ISO 3 Code	DEU
[Ref.: #17341]	Continent	Europe

[Ref.: #62192]	Sample type/isolated from	Leaf phyllosphere of Cerastium holosteoides
[Ref.: #62192]	Sampling date	2008
[Ref.: #62192]	Geographic location (country and/or sea, region)	Thüringen
[Ref.: #62192]	Country	Germany
[Ref.: #62192]	Country ISO 3 Code	DEU
[Ref.: #62192]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Leaf (Phyllosphere)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FR733885 (>99% sequence identity) for Methylobacterium from Microbeatlas

Aquatic Samples	353
Soil Samples	698
Animal Samples	1785
Plant Samples	1121
Total Samples	3957

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17341]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #17341]	Methylobacterium cerastii partial 16S rRNA gene, isolate C15	FR733885	1353	GenBank ENA	932741 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methylobacterium cerastii DSM 23679	GCA_022179125	contig	GenBank ENA	932741 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methylobacterium cerastii DSM 23679 NCBI: GCA_022179125.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	97.802	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	92.302	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	87.097	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	86.931	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	97.999	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	71.68	no

Availability in culture collections External links

[Ref.: #17341]

Culture collection no.

DSM 23679, CCM 7788, CCUG 60040

[Ref.: #76650]

SI-ID 379629

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methylobacterium cerastii sp. nov., isolated from the leaf surface of Cerastium holosteoides.	Wellner S, Lodders N, Kampfer P	Int J Syst Evol Microbiol	10.1099/ijs.0.030767-0	2011	*

References

#17341

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23679

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30207

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26552 (see below)

#62192 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 60040

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76650

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID379629.1 )

#26552

IJSEM 917 2012 ( DOI 10.1099/ijs.0.030767-0 , PubMed 21669927 )

* These data were automatically processed and therefore are not curated

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Methylobacterium cerastii DSM 23679

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