Name: Methylobacterium hispanicum GP34
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7175

Type strain:

Species: Methylobacterium hispanicum

Strain Designation: GP34

Culture col. no.: DSM 16372, CCM 7219, CECT 5997, CIP 108332

Strain history: CIP <- 2004, A. Ventosa, Sevilla Univ., Sevilla, Spain: strain GP34

NCBI tax ID(s): 270350 (species)

SI-ID 135121

CCM 7219, CECT 5997, CIP 108332, DSM 16372

Section

Methylobacterium hispanicum GP34 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from drinking water.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Methylobacteriaceae*
	Genus	*Methylobacterium*
	Species	**Methylobacterium hispanicum**
	Full Scientific Name (LPSN)	Methylobacterium hispanicum Gallego et al. 2005

Information on morphological and physiological properties Morphology

[Ref.: #31326]	Gram stain	negative
[Ref.: #119920]	Gram stain	negative

[Ref.: #31326]	Cell length	2.25 µm

[Ref.: #31326]	Cell width	1.25 µm

[Ref.: #31326]	Cell shape	rod-shaped
[Ref.: #119920]	Cell shape	rod-shaped

[Ref.: #31326]	Motility	yes
[Ref.: #119920]	Motility	yes

[Ref.: #31326]	Pigment production	yes
[Ref.: #119920]	Pigment production	no
[Ref.: #119920]	Pigment name	Pyocyanin

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6409]

Culture medium

PLATE COUNT AGAR (DSMZ Medium 464)

[Ref.: #6409]

Culture medium growth

[Ref.: #6409]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6409]

Culture medium composition

expand / minimize

[Ref.: #33944]

Culture medium

MEDIUM 229 - for Methylobacterium aminovorans

[Ref.: #33944]

Culture medium growth

[Ref.: #33944]

Culture medium composition

expand / minimize

[Ref.: #119920]

Culture medium

CIP Medium 229

[Ref.: #119920]

Culture medium growth

[Ref.: #119920]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #6409]		growth	28 °C
[Ref.: #31326]		growth	15-30 °C
[Ref.: #31326]		optimum	28 °C
[Ref.: #33944]		growth	25 °C
[Ref.: #119920]		growth	25-30 °C
[Ref.: #119920]	no	growth	5 °C
[Ref.: #119920]	no	growth	10 °C
[Ref.: #119920]	no	growth	37 °C
[Ref.: #119920]	no	growth	41 °C

[Ref.: #6409]	Temperature range	mesophilic
[Ref.: #31326]	Temperature range	mesophilic
[Ref.: #33944]	Temperature range	mesophilic
[Ref.: #119920]	Temperature range	mesophilic
[Ref.: #119920]	Temperature range	psychrophilic
[Ref.: #119920]	Temperature range	mesophilic
[Ref.: #119920]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #31326]			optimum	6.5
[Ref.: #31326]			growth	5.0-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31326]

Oxygen tolerance

aerobe

[Ref.: #31326]

Ability of spore formation

	Salt	Tested relation		Salt conc.
[Ref.: #31326]	NaCl		growth	0.0-1.0	%
[Ref.: #31326]	NaCl		optimum	0.5	%
[Ref.: #119920]	NaCl		growth	0-4	%
[Ref.: #119920]	NaCl	no	growth	6	%
[Ref.: #119920]	NaCl	no	growth	8	%
[Ref.: #119920]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31326]	acetate ChEBI	+	carbon source
[Ref.: #31326]	arabinose ChEBI	+	carbon source
[Ref.: #31326]	asparagine ChEBI	+	carbon source
[Ref.: #31326]	citrate ChEBI	+	carbon source
[Ref.: #119920]	citrate ChEBI	+	carbon source
[Ref.: #119920]	esculin ChEBI	-	hydrolysis
[Ref.: #31326]	formate ChEBI	+	carbon source
[Ref.: #31326]	fructose ChEBI	+	carbon source
[Ref.: #31326]	glutamate ChEBI	+	carbon source
[Ref.: #31326]	glycerol ChEBI	+	carbon source
[Ref.: #31326]	lactate ChEBI	+	carbon source
[Ref.: #31326]	malate ChEBI	+	carbon source
[Ref.: #31326]	methyl pyruvate ChEBI	+	carbon source
[Ref.: #31326]	nitrate ChEBI	+	reduction
[Ref.: #119920]	nitrate ChEBI	-	reduction
[Ref.: #119920]	nitrate ChEBI	-	respiration
[Ref.: #119920]	nitrite ChEBI	-	reduction
[Ref.: #31326]	propionate ChEBI	+	carbon source
[Ref.: #31326]	pyruvate ChEBI	+	carbon source
[Ref.: #31326]	succinate ChEBI	+	carbon source
[Ref.: #31326]	tween 40 ChEBI	+	carbon source
[Ref.: #31326]	tween 80 ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #119920]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #119920]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #119920]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #119920]	amylase	-
[Ref.: #119920]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #119920]	caseinase	-	3.4.21.50
[Ref.: #31326]	catalase	+	1.11.1.6
[Ref.: #119920]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #31326]	cytochrome oxidase	+	1.9.3.1
[Ref.: #119920]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #119920]	gelatinase	-
[Ref.: #119920]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #119920]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #119920]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119920]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #119920]	oxidase	+
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #119920]	tryptophan deaminase	-
[Ref.: #119920]	tween esterase	-
[Ref.: #31326]	urease	+	3.5.1.5
[Ref.: #119920]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #119920]	2708	not determinedn.d.	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	-	-	-	+	+	+	-	+	+	-	-	+	-	+	-	+	-	+	-	-	+	-	-	-	-	-	-	+	+	-	-	-	-	+	-	-	-	+	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	+	+	-	+	-	-	-	+	+	+	+	+	-	-	-	-	-	-	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119920]	11097	-	-	+	+	-	+	+	-	+	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6409]	Sample type/isolated from	drinking water
[Ref.: #6409]	Geographic location (country and/or sea, region)	Seville
[Ref.: #6409]	Country	Spain
[Ref.: #6409]	Country ISO 3 Code	ESP
[Ref.: #6409]	Continent	Europe

[Ref.: #119920]	Sample type/isolated from	Environment, Drinking water
[Ref.: #119920]	Geographic location (country and/or sea, region)	Sevilla
[Ref.: #119920]	Country	Spain
[Ref.: #119920]	Country ISO 3 Code	ESP
[Ref.: #119920]	Continent	Europe
[Ref.: #119920]	Isolation date	2003
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Freshwater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ635304 (>99% sequence identity) for Methylobacterium hispanicum subclade from Microbeatlas

Aquatic Samples	3860
Soil Samples	2118
Animal Samples	7029
Plant Samples	2987
Total Samples	15994

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6409]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119920]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6409]	Methylobacterium hispanicum 16S rRNA gene, strain type strain GP34T	AJ635304	1408	ENA	270350 tax ID
[Ref.: #20218]	Methylobacterium hispanicum gene for 16S rRNA, partial sequence, strain: DSM 16372	AB252198	1433	ENA	270350 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methylobacterium hispanicum DSM 16372	GCA_022179285	contig	GenBank	270350 tax ID	*

[Ref.: #6409]	GC-content	67.5 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #31326]	GC-content	67.5 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methylobacterium hispanicum DSM 16372 NCBI: GCA_022179285.1
[Ref.: #69480]	flagellated	no	73.688	no
[Ref.: #69480]	gram-positive	no	97.145	no
[Ref.: #69480]	anaerobic	no	97.147	yes
[Ref.: #69480]	aerobic	yes	88.758	yes
[Ref.: #69480]	halophile	no	91.515	no
[Ref.: #69480]	spore-forming	no	92.771	yes
[Ref.: #69480]	motile	yes	87.008	yes
[Ref.: #69480]	glucose-ferment	no	90.233	no
[Ref.: #69480]	thermophile	no	98.701	no
[Ref.: #69480]	glucose-util	yes	81.486	no

Availability in culture collections External links

[Ref.: #6409]

Culture collection no.

DSM 16372, CCM 7219, CECT 5997, CIP 108332

[Ref.: #76628]

SI-ID 135121

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methylobacterium hispanicum sp. nov. and Methylobacterium aquaticum sp. nov., isolated from drinking water.	Gallego V, Garcia MT, Ventosa A	Int J Syst Evol Microbiol	10.1099/ijs.0.63319-0	2005	*

References

#6409

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16372

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#31326

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27640 (see below)

#33944 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76628

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID135121.1 )

#119920 Collection of Institut Pasteur ; Curators of the CIP; CIP 108332

#27640

IJSEM 281 2005 ( DOI 10.1099/ijs.0.63319-0 , PubMed 15653888 )

* These data were automatically processed and therefore are not curated

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Methylobacterium hispanicum DSM 16372

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