Name: Methylorubrum thiocyanatum ALL/SCN-P
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7172

Type strain:

Species: Methylorubrum thiocyanatum

Strain Designation: ALL/SCN-P

Culture col. no.: DSM 11490, ATCC 700647, JCM 10893, NCIMB 13651, VKM B-2197

Strain history: NCIMB 13651 <-- DSM 11490 <-- D. P. Kelly strain ALL/SCN-P.

NCBI tax ID(s): 1236972 (strain), 47958 (species)

Section

Methylorubrum thiocyanatum ALL/SCN-P is a mesophilic bacterium that was isolated from soil around root balls of Allium aflatunense.

mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Methylobacteriaceae*
	Genus	*Methylorubrum*
	Species	**Methylorubrum thiocyanatum**
	Full Scientific Name (PNU)	Methylorubrum thiocyanatum (Wood et al. 1999) Green and Ardley 2018

Synonym

Methylobacterium thiocyanatum

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %100

[Ref.: #69480]	Motility	yes Confidence in %90.965

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4386]

Culture medium

METHYLOBACTERIUM THIOCYANATUM MEDIUM (DSMZ Medium 805)

[Ref.: #4386]

Culture medium growth

[Ref.: #4386]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #4386]

Culture medium composition

expand / minimize

[Ref.: #4386]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #4386]

Culture medium growth

[Ref.: #4386]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #4386]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #4386]			growth	30 °C
[Ref.: #67770]			growth	30 °C

[Ref.: #4386]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.999

[Ref.: #67770]

Observation

quinones: Q-10

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4386]	Sample type/isolated from	soil around root balls of Allium aflatunense
[Ref.: #4386]	Host species	Allium aflatunense
[Ref.: #4386]	Geographic location (country and/or sea, region)	Warwickshire
[Ref.: #4386]	Country	United Kingdom
[Ref.: #4386]	Country ISO 3 Code	GBR
[Ref.: #4386]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Soil from around the roots of Allium aflatunense
[Ref.: #67770]	Host species	Allium aflatunense
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root (Rhizome)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence U58018 (>99% sequence identity) for Methylorubrum from Microbeatlas

Aquatic Samples	23711
Soil Samples	23280
Animal Samples	47578
Plant Samples	16159
Total Samples	110728

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4386]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Methylobacterium thiocyanatum 16S ribosomal RNA gene, complete sequence	U58018	1447	ENA	47958 tax ID	*
[Ref.: #20218]	Methylobacterium thiocyanatum gene for 16S ribosomal RNA, partial sequence, strain:DSM 11490	AB175646	1435	ENA	47958 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methylorubrum thiocyanatum JCM 10893 JCM 10893	GCA_001310875	contig	GenBank	1236972 tax ID	*
[Ref.: #66792]	Methylobacterium thiocyanatum JCM 10893	1236972.3	wgs	PATRIC	1236972 tax ID	*
[Ref.: #66792]	Methylorubrum thiocyanatum strain DSM 11490	47958.4	wgs	PATRIC	47958 tax ID	*
[Ref.: #66792]	Methylorubrum thiocyanatum JCM 10893	2728369660	draft	JGI IMG/M	1236972 tax ID	*
[Ref.: #66792]	Methylobacterium thiocyanatum DSM 11490	2861691609	draft	JGI IMG/M	47958 tax ID	*
[Ref.: #66792]	Methylorubrum thiocyanatum JCM 10893	GCA_022179785	contig	GenBank	47958 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methylorubrum thiocyanatum strain DSM 11490 PATRIC: 47958.4
[Ref.: #69480]	spore-forming	no	99.999	no
[Ref.: #69480]	anaerobic	no	95.95	no
[Ref.: #69480]	gram-positive	no	100	no
[Ref.: #69480]	thermophile	no	99.876	yes
[Ref.: #69480]	motile	yes	90.965	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Methylorubrum thiocyanatum strain DSM 11490 PATRIC: 47958.4
[Ref.: #69481]	spore-forming	no	100	no

Availability in culture collections External links

[Ref.: #4386]

Culture collection no.

DSM 11490, ATCC 700647, JCM 10893, NCIMB 13651, VKM B-2197

[Ref.: #20218]

Associated Passport(s) in StrainInfo

839949, 122010

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Review of the genus Methylobacterium and closely related organisms: a proposal that some Methylobacterium species be reclassified into a new genus, Methylorubrum gen. nov.	Green PN, Ardley JK	Int J Syst Evol Microbiol	10.1099/ijsem.0.002856	2018	*
Phylogeny	Methylobacterium pseudosasae sp. nov., a pink-pigmented, facultatively methylotrophic bacterium isolated from the bamboo phyllosphere.	Madhaiyan M, Poonguzhali S	Antonie Van Leeuwenhoek	10.1007/s10482-013-0085-0	2013	*
Metabolism	A novel pink-pigmented facultative methylotroph, Methylobacterium thiocyanatum sp. nov., capable of growth on thiocyanate or cyanate as sole nitrogen sources.	Wood AP, Kelly DP, McDonald IR, Jordan SL, Morgan TD, Khan S, Murrell JC, Borodina E	Arch Microbiol	10.1007/s002030050554	1998	*

References

#4386

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 11490

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Methylorubrum thiocyanatum strain DSM 11490

Methylorubrum thiocyanatum strain DSM 11490

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