Methylobacterium organophilum (DSM 18172, ATCC 27886, CIP 101049)

Name: Methylobacterium organophilum (DSM 18172, ATCC 27886, CIP 101049)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7151

Type strain

Strain Designation XX

Culture col. no. DSM 18172 ATCC 27886 CIP 101049 IFO 15689 JCM 2833 3 more

NCBI tax ID(s) 410

Special Collections DSMZ JCM CIP

History DSMZ <- NCIMB <- ATCC <- R. S. Hanson. NOt identical to DSM 760, a strain directly obtained from the laboratory where the strain was isolated CIP <- 1984, NCIB <- ATCC <- R.S. Hanson: strain XX

Links

Methylobacterium organophilum XX is a Gram-negative, rod-shaped bacterium that was isolated from sediment.

Gram-negative rod-shaped 16S sequence Bacteria

Name and taxonomic classification

SI-ID 3561

LMG 6083,ATCC 27886,DSM 760,JCM 2833,NCIB 11278,CIP 101049,CCRC 10934,IFO 15689,NCIMB 11278,CCM 4460,VKM B-2066,NBRC 15689,NCCB 78041,LMD 78.41,HAMBI 2263,BCRC 10934,DSM 18172,CCUG 55902

@ref 20215

Last LPSN update 2026-05-29 08:26:35

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Hyphomicrobiales

Family Methylobacteriaceae

Genus Methylobacterium

Species Methylobacterium organophilum

Full scientific name Methylobacterium organophilum Patt et al. 1976 (Approved Lists 1980)

BacDive ID	Other strains from **Methylobacterium organophilum** (4)	Type strain
7150	M. organophilum XX, DSM 760
136034	M. organophilum 998-85, CIP 102028
138029	M. organophilum 535-86, CIP 102545
149309	M. organophilum CCUG 37468

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
121432	negative	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
7410	METHYLOBACTERIUM MEDIUM (DSMZ Medium 125)		Medium recipe at MediaDive	Name: METHYLOBACTERIUM MEDIUM (DSMZ Medium 125) Composition: Agar 12.0 g/l KNO3 1.0 g/l Na2HPO4 0.23 g/l MgSO4 x 7 H2O 0.2 g/l NaH2PO4 0.07 g/l CaCl2 x 2 H2O 0.02 g/l FeSO4 x 7 H2O 0.001 g/l ZnSO4 x 7 H2O 7e-05 g/l H3BO3 1e-05 g/l MnSO4 x 5 H2O 1e-05 g/l MoO3 1e-05 g/l CuSO4 x 5 H2O 5e-06 g/l Distilled water
7410	NUTRIENT AGAR (DSMZ Medium 1)		Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1; with strain-specific modifications) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Methanol Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
7410	positive	growth	30

Physiology and metabolism

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Sediment
#Environmental	#Terrestrial	#Sediment

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
7410	sediment	Wisconsin, Madison, Lake Mendota	USA	USA	North America
121432	Environment, Sediment	Mendota lake, Madison	United States of America	USA	North America

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
7410	1	Risk group (German classification) According to TRBA 466
121432	1	Risk group (French classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Methylobacterium organophilum 16S ribosomal RNA gene, partial sequence; tRNA-Ile and tRNA-Ala genes, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF338181	1163	410
20218	Methylobacterium organophilum partial 16S rRNA gene, strain JCM 2833	AJ400920	697	410
7410	Methylobacterium organophilum partial 16S rRNA gene, type strain DSM 18172T	LN681562	1435	410

GC content

7410

GC-content (mol%)66.0

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Methylobacterium as a key symbiont in plant-microbe interactions: Its ecological and agricultural significance.	Grossi CEM, Ulloa RM, Sahin N, Tani A.	Plant Biotechnol (Tokyo)	10.5511/plantbiotechnology.25.0309a	2025
	Genetic evidence for algal auxin production in Chlamydomonas and its role in algal-bacterial mutualism	Calatrava V, Hom E, Guan Q, Llamas A, Fernandez E, Galvan A.	iScience		2023
	Construction of broad-host-range cosmid cloning vectors: identification of genes necessary for growth of Methylobacterium organophilum on methanol.	Allen LN, Hanson RS.	J Bacteriol	10.1128/jb.161.3.955-962.1985	1985
	Extension of the model concerning linkage of genes coding for C-1 related functions in Methylobacterium organophilum.	O'Connor ML.	Appl Environ Microbiol	10.1128/aem.41.2.437-441.1981	1981
	Organization of Genes Required for the Oxidation of Methanol to Formaldehyde in Three Type II Methylotrophs.	Bastien C, Machlin S, Zhang Y, Donaldson K, Hanson RS.	Appl Environ Microbiol	10.1128/aem.55.12.3124-3130.1989	1989
Metabolism	Genetic and physical analyses of Methylobacterium organophilum XX genes encoding methanol oxidation.	Machlin SM, Tam PE, Bastien CA, Hanson RS.	J Bacteriol	10.1128/jb.170.1.141-148.1988	1988
	Nucleotide sequence and transcriptional start site of the Methylobacterium organophilum XX methanol dehydrogenase structural gene.	Machlin SM, Hanson RS.	J Bacteriol	10.1128/jb.170.10.4739-4747.1988	1988
	Isolation and nucleotide sequence of the methanol dehydrogenase structural gene from Paracoccus denitrificans.	Harms N, de Vries GE, Maurer K, Hoogendijk J, Stouthamer AH.	J Bacteriol	10.1128/jb.169.9.3969-3975.1987	1987
Enzymology	Alcohol dehydrogenase from Methylobacterium organophilum.	Wolf HJ, Hanson RS.	Appl Environ Microbiol	10.1128/aem.36.1.105-114.1978	1978
Phylogeny	Evaluation of the Biolog automated microbial identification system.	Klingler JM, Stowe RP, Obenhuber DC, Groves TO, Mishra SK, Pierson DL.	Appl Environ Microbiol	10.1128/aem.58.6.2089-2092.1992	1992
Enzymology	Characterization of the methanotrophic bacterial community present in a trichloroethylene-contaminated subsurface groundwater site.	Bowman JP, Jimenez L, Rosario I, Hazen TC, Sayler GS.	Appl Environ Microbiol	10.1128/aem.59.8.2380-2387.1993	1993
	Genetic diversity of rhizobial symbionts isolated from legume species within the genera Astragalus, Oxytropis, and Onobrychis.	Laguerre G, van Berkum P, Amarger N, Prevost D.	Appl Environ Microbiol	10.1128/aem.63.12.4748-4758.1997	1997
Metabolism	A survey of Methylobacterium species and strains reveals widespread production and varying profiles of cytokinin phytohormones.	Palberg D, Kisiala A, Jorge GL, Emery RJN.	BMC Microbiol	10.1186/s12866-022-02454-9	2022
Metabolism	Bacterial community dynamics during start-up of a trickle-bed bioreactor degrading aromatic compounds.	Stoffels M, Amann R, Ludwig W, Hekmat D, Schleifer KH.	Appl Environ Microbiol	10.1128/aem.64.3.930-939.1998	1998
Phylogeny	Methylobacterium terricola sp. nov., a gamma radiation-resistant bacterium isolated from gamma ray-irradiated soil.	Kim J, Chhetri G, Kim I, Lee B, Jang W, Kim MK, Seo T	Int J Syst Evol Microbiol	10.1099/ijsem.0.004054	2020
Phylogeny	Methylobacterium terrae sp. nov., a radiation-resistant bacterium isolated from gamma ray-irradiated soil.	Kim J, Chhetri G, Kim I, Kim H, Kim MK, Seo T	J Microbiol	10.1007/s12275-019-9007-9	2019
Phylogeny	Psychroglaciecola arctica gen. nov., sp. nov., isolated from Arctic glacial foreland soil.	Qu Z, Jiang F, Chang X, Qiu X, Ren L, Fang C, Peng F	Int J Syst Evol Microbiol	10.1099/ijs.0.060913-0	2014
Phylogeny	Methylobacterium haplocladii sp. nov. and Methylobacterium brachythecii sp. nov., isolated from bryophytes.	Tani A, Sahin N	Int J Syst Evol Microbiol	10.1099/ijs.0.048215-0	2013
Phylogeny	Methylobacterium trifolii sp. nov. and Methylobacterium thuringiense sp. nov., methanol-utilizing, pink-pigmented bacteria isolated from leaf surfaces.	Wellner S, Lodders N, Glaeser SP, Kampfer P	Int J Syst Evol Microbiol	10.1099/ijs.0.047787-0	2013
Phylogeny	Methylobacterium gnaphalii sp. nov., isolated from leaves of Gnaphalium spicatum.	Tani A, Sahin N, Kimbara K	Int J Syst Evol Microbiol	10.1099/ijs.0.037713-0	2011
Phylogeny	Methylobacterium jeotgali sp. nov., a non-pigmented, facultatively methylotrophic bacterium isolated from jeotgal, a traditional Korean fermented seafood.	Aslam Z, Lee CS, Kim KH, Im WT, Ten LN, Lee ST	Int J Syst Evol Microbiol	10.1099/ijs.0.64625-0	2007
Phylogeny	Bosea minatitlanensis sp. nov., a strictly aerobic bacterium isolated from an anaerobic digester.	Ouattara AS, Assih EA, Thierry S, Cayol JL, Labat M, Monroy O, Macarie H	Int J Syst Evol Microbiol	10.1099/ijs.0.02540-0	2003

References

#7410	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18172
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#121432	Collection of Institut Pasteur ; Curators of the CIP; CIP 101049
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methylobacterium organophilum (DSM 18172, ATCC 27886, CIP 101049)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

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